Tyr1250

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Phosphorylation Site Page:

Tyr1250 - HER2 (rat)

Site Information

PTAENPEyLGLDVPV SwissProt Entrez-Gene

Predicted information: Scansite

Orthologous residues: HER2 (human): Y1248, HER2 (mouse): Y1249, HER2 iso2 (human): Y638

Blast this site against: NCBI SwissProt PDB

In vivo Characterization

Methods used to characterize site in vivo:	flow cytometry (1), microscopy-colocalization with upstream kinase (7), mutation of modification site (1, 6, 7, 9), phospho-antibody (1, 2, 3, 4, 5, 8), western blotting (1, 2, 3, 7)
Disease tissue studied:	breast cancer (1, 7)
Relevant cell line - cell type - tissue:	'muscle, smooth'-thoracic aorta (5), 3T3 (fibroblast) (9), cholangiocyte (4), COS (fibroblast) (7), G8/DHFR (fibroblast) (8), heart (2), MCF-10A (breast cell) (1), MCF-7 (breast cell) (7), MDCK (epithelial) (1), Rat1 (fibroblast) (6), Schwann (3, 8)

Controlled by

Putative upstream kinases:	HER2 (rat) (1)
Treatments:	AG825 (3), AP1510 (1), betacellulin (5), EGF (7), heart failure (2), laminin-1 (3), nerve damage (8), neuregulin (2, 3), phorbol ester (8)

Downstream Regulation

Effects of modification on HER2:	molecular association, regulation (5, 7)
Effects of modification on biological processes:	cell growth, altered (9)
Induce interaction with:	Chk1 (rat) (7), EGFR (rat) (5)

Disease / Diagnostics Relevance

Relevant diseases:

breast cancer (7)

	References

1	Lucs AV, Muller WJ, Muthuswamy SK (2010) Shc is required for ErbB2-induced inhibition of apoptosis but is dispensable for cell proliferation and disruption of cell polarity. Oncogene 29, 174-87 19826412 Curated Info
2	Doggen K, et al. (2009) Ventricular ErbB2/ErbB4 activation and downstream signaling in pacing-induced heart failure. J Mol Cell Cardiol 46, 33-8 19010331 Curated Info
3	Thaxton C, Lopera J, Bott M, Fernandez-Valle C (2008) Neuregulin and laminin stimulate phosphorylation of the NF2 tumor suppressor in Schwann cells by distinct protein kinase A and p21-activated kinase-dependent pathways. Oncogene 27, 2705-15 17998937 Curated Info
4	Lai GH, et al. (2005) erbB-2/neu transformed rat cholangiocytes recapitulate key cellular and molecular features of human bile duct cancer. Gastroenterology 129, 2047-57 16344070 Curated Info
5	Mifune M, et al. (2004) Signal transduction of betacellulin in growth and migration of vascular smooth muscle cells. Am J Physiol Cell Physiol 287, C807-13 15163624 Curated Info
6	Dankort D, et al. (2001) Multiple ErbB-2/Neu Phosphorylation Sites Mediate Transformation through Distinct Effector Proteins. J Biol Chem 276, 38921-8 11500516 Curated Info
7	Zrihan-Licht S, et al. (1998) Csk homologous kinase, a novel signaling molecule, directly associates with the activated ErbB-2 receptor in breast cancer cells and inhibits their proliferation. J Biol Chem 273, 4065-72 9461599 Curated Info
8	Kwon YK, et al. (1997) Activation of ErbB2 during wallerian degeneration of sciatic nerve. J Neurosci 17, 8293-9 9334404 Curated Info
9	Mikami Y, et al. (1992) Carboxyl-terminal deletion and point mutations decrease the transforming potential of the activated rat neu oncogene product. Proc Natl Acad Sci U S A 89, 7335-9 1354355 Curated Info

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