Ser74
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Phosphorylation Site Page:
Ser74 - dCK (human)

Site Information
EFEELTMsQKNGGNV    SwissProt Entrez-Gene
Predicted information: Scansite
Orthologous residues: dCK (mouse): S74, dCK (rat): S74
Blast this site against: NCBI  SwissProt  PDB 
Available spectra:  1 CST

In vivo Characterization
Methods used to characterize site in vivo: [32P] bio-synthetic labeling (82, 89), immunoprecipitation (21), mass spectrometry (3, 4, 5, 6, 7, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 22, 24, 25, 26, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 57, 58, 59, 60, 61, 62, 63, 64, 65, 68, 69, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101), mass spectrometry (in vitro) (21), mutation of modification site (8, 66, 82, 89), phospho-antibody (21, 28, 70, 82), phosphopeptide mapping (21, 89), western blotting (28, 70)
Disease tissue studied: brain cancer (93, 94, 95, 98, 99, 100, 101), glioblastoma (93, 94, 95, 98, 99, 100, 101), glioma (93, 94, 95, 98, 99, 100, 101), cervical cancer (41), cervical adenocarcinoma (41), leukemia (21, 65, 70), chronic lymphocytic leukemia (21, 70), chronic myelogenous leukemia (65), T cell leukemia (21, 70), multiple myeloma (33)
Relevant cell line - cell type - tissue: 293 (epithelial) (66), 293T (epithelial) (21, 28, 71, 82, 89, 96, 97), B lymphocyte (21, 70), B lymphocyte-blood (33), CCRF-CEM (T lymphocyte) (21, 70, 82), EHEB (B lymphocyte) (21), GM00200 (fibroblast) (74, 75), GM18366 (fibroblast) (76, 77), HCT116 (intestinal) (46), HeLa (cervical) (4, 5, 6, 7, 47, 48, 49, 50, 60, 61, 62, 63, 64), HeLa S3 (cervical) (41, 59), Jurkat (T lymphocyte) (3, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 22, 24, 25, 26, 29, 30, 31, 32, 34, 35, 36, 37, 38, 39, 40, 42, 43, 44, 45, 51, 52, 53, 54, 68, 69, 72, 73), K562 (erythroid) (65), M059J (glial) (86, 88, 94), M059K (glial) (78, 79, 80, 81, 83, 84, 85, 87, 90, 91, 92, 93, 95, 98, 99, 100, 101), MDA-MB-435S (breast cell) (46), MKN-45 (gastric) (57, 58), MV4-11 (myeloid) (46)

Controlled by
Kinases, in vitro: CK1-D (human) (21)
Treatments: aphidicolin (21, 70), CdA (70), CK1-7 (21), etoposide (70), genistein (70, 82), IC261 (21), okadaic acid (21), osmotic stress (70), siRNA (21), sorbitol (21, 82), UV (70, 82)

Downstream Regulation
Effects of modification on dCK: enzymatic activity, induced (21, 28, 66, 82, 89), protein conformation (8)


References

1

Yang C, et al. (2012) Deoxycytidine kinase regulates the G2/M checkpoint through interaction with cyclin-dependent kinase 1 in response to DNA damage. Nucleic Acids Res
22850745   Curated Info

2

Amsailale R, et al. (2012) Phosphorylation of deoxycytidine kinase on Ser-74: Impact on kinetic properties and nucleoside analog activation in cancer cells. Biochem Pharmacol 84, 43-51
22490700   Curated Info

3

(2011) CST Curation Set: 12070; Year: 2011; Biosample: Jurkat; Disease: T cell leukemia; Treatment: calyculin & pervanadate; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(F/Y/M)X(s/t)(L/I/M(phosphorylation)
Curated Info

4

(2011) CST Curation Set: 11707; Year: 2011; Biosample: HeLa; Disease: cervical adenocarcinoma; Treatment: UV; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-(Ser/Thr) ATM/ATR Substrate (S*/T*QG) (P-S/T2-100) Rabbit mAb Cat#: 6966, PTMScan(R) Phospho-ATM/ATR Substrate Motif (S*/T*QG) Immunoaffinity Beads Cat#: 6969
Curated Info

5

(2011) CST Curation Set: 11708; Year: 2011; Biosample: HeLa; Disease: cervical adenocarcinoma; Treatment: UV; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-(Ser/Thr) ATM/ATR Substrate (S*/T*QG) (P-S/T2-100) Rabbit mAb Cat#: 6966, PTMScan(R) Phospho-ATM/ATR Substrate Motif (S*/T*QG) Immunoaffinity Beads Cat#: 6969
Curated Info

6

(2011) CST Curation Set: 11709; Year: 2011; Biosample: HeLa; Disease: cervical adenocarcinoma; Treatment: UV; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-ATM/ATR Substrate (S*Q) (D23H2/D69H5) Rabbit mAb Cat#: 9607, PTMScan(R) Phospho-ATM/ATR Substrate (S*Q) Immunoaffinity Beads Cat#: 9884
Curated Info

7

(2011) CST Curation Set: 11710; Year: 2011; Biosample: HeLa; Disease: cervical adenocarcinoma; Treatment: UV; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-ATM/ATR Substrate (S*Q) (D23H2/D69H5) Rabbit mAb Cat#: 9607, PTMScan(R) Phospho-ATM/ATR Substrate (S*Q) Immunoaffinity Beads Cat#: 9884
Curated Info

8

Hazra S, et al. (2011) Post-translational phosphorylation of serine 74 of human deoxycytidine kinase favors the enzyme adopting the open conformation making it competent for nucleoside binding and release. Biochemistry 50, 2870-80
21351740   Curated Info

9

(2011) CST Curation Set: 11634; Year: 2011; Biosample: Jurkat; Disease: T cell leukemia; Treatment: calyculin & pervanadate; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho(Ser/Thr) ATM/ATR Substrate Antibody (polyAB) Cat#: 3005, PTMScan(R) Phospho-ATM/ATR Substrate Motif (pS/pTQ) Immunoaffinity Beads Cat#: 1980
Curated Info

10

(2011) CST Curation Set: 11523; Year: 2011; Biosample: Jurkat; Disease: T cell leukemia; Treatment: calyculin & pervanadate; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-ATM/ATR Substrate (S*Q) (D23H2/D69H5) Rabbit mAb Cat#: 9607, PTMScan(R) Phospho-ATM/ATR Substrate (S*Q) Immunoaffinity Beads Cat#: 9884
Curated Info

11

(2011) CST Curation Set: 11524; Year: 2011; Biosample: Jurkat; Disease: T cell leukemia; Treatment: calyculin & pervanadate; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-ATM/ATR Substrate (S*Q) (D23H2/D69H5) Rabbit mAb Cat#: 9607, PTMScan(R) Phospho-ATM/ATR Substrate (S*Q) Immunoaffinity Beads Cat#: 9884
Curated Info

12

(2011) CST Curation Set: 11376; Year: 2011; Biosample: Jurkat; Disease: T cell leukemia; Treatment: calyculin & pervanadate; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation)
Curated Info

13

(2011) CST Curation Set: 11378; Year: 2011; Biosample: Jurkat; Disease: T cell leukemia; Treatment: calyculin & pervanadate; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation)
Curated Info

14

(2011) CST Curation Set: 11379; Year: 2011; Biosample: Jurkat; Disease: T cell leukemia; Treatment: calyculin & pervanadate; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho(Ser/Thr) ATM/ATR Substrate Antibody (polyAB) Cat#: 3005, PTMScan(R) Phospho-ATM/ATR Substrate Motif (pS/pTQ) Immunoaffinity Beads Cat#: 1980
Curated Info

15

(2011) CST Curation Set: 11380; Year: 2011; Biosample: Jurkat; Disease: T cell leukemia; Treatment: calyculin & pervanadate; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation)
Curated Info

16

(2011) CST Curation Set: 11381; Year: 2011; Biosample: Jurkat; Disease: T cell leukemia; Treatment: calyculin & pervanadate; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation)
Curated Info

17

(2011) CST Curation Set: 11382; Year: 2011; Biosample: Jurkat; Disease: T cell leukemia; Treatment: calyculin & pervanadate; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation)
Curated Info

18

(2011) CST Curation Set: 11116; Year: 2011; Biosample: Jurkat; Disease: T cell leukemia; Treatment: calyculin & pervanadate; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho(Ser/Thr) ATM/ATR Substrate Antibody (polyAB) Cat#: 3005, PTMScan(R) Phospho-ATM/ATR Substrate Motif (pS/pTQ) Immunoaffinity Beads Cat#: 1980
Curated Info

19

(2011) CST Curation Set: 11115; Year: 2011; Biosample: Jurkat; Disease: T cell leukemia; Treatment: calyculin & pervanadate; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho(Ser/Thr) ATM/ATR Substrate Antibody (polyAB) Cat#: 3005, PTMScan(R) Phospho-ATM/ATR Substrate Motif (pS/pTQ) Immunoaffinity Beads Cat#: 1980
Curated Info

20

(2010) CST Curation Set: 10867; Year: 2010; Biosample: Jurkat; Disease: T cell leukemia; Treatment: pervanadate & calyculin; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(F/Y/M)X(s/t)(L/I/M)(phosphorylation)
Curated Info

21

Smal C, et al. (2010) Casein kinase 1delta activates human recombinant deoxycytidine kinase by Ser-74 phosphorylation, but is not involved in the in vivo regulation of its activity. Arch Biochem Biophys 502, 44-52
20637175   Curated Info

22

(2010) CST Curation Set: 10303; Year: 2010; Biosample: Jurkat; Disease: T cell leukemia; Treatment: pervanadate & calyculin; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho(Ser/Thr) ATM/ATR Substrate Antibody (polyAB) Cat#: 3005, PTMScan(R) Phospho-ATM/ATR Substrate Motif (pS/pTQ) Immunoaffinity Beads Cat#: 1980
Curated Info

23

(2010) CST Curation Set: 10304; Year: 2010; Biosample: Jurkat; Disease: T cell leukemia; Treatment: pervanadate & calyculin; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho(Ser/Thr) ATM/ATR Substrate Antibody (polyAB) Cat#: 3005, PTMScan(R) Phospho-ATM/ATR Substrate Motif (pS/pTQ) Immunoaffinity Beads Cat#: 1980
Curated Info

24

(2010) CST Curation Set: 10270; Year: 2010; Biosample: Jurkat; Disease: T cell leukemia; Treatment: pervanadate & calyculin; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)P(phosphorylation)
Curated Info

25

(2010) CST Curation Set: 9961; Year: 2010; Biosample: Jurkat; Disease: T cell leukemia; Treatment: pervanadate & calyculin; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation)
Curated Info

26

(2010) CST Curation Set: 9960; Year: 2010; Biosample: Jurkat; Disease: T cell leukemia; Treatment: pervanadate & calyculin; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation)
Curated Info

27

(2010) CST Curation Set: 9962; Year: 2010; Biosample: Jurkat; Disease: T cell leukemia; Treatment: pervanadate & calyculin; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation)
Curated Info

28

Smal C, et al. (2010) Influence of phosphorylation of THR-3, SER-11, and SER-15 on deoxycytidine kinase activity and stability. Nucleosides Nucleotides Nucleic Acids 29, 404-7
20544527   Curated Info

29

(2010) CST Curation Set: 9643; Year: 2010; Biosample: Jurkat; Disease: T cell leukemia; Treatment: pervanadate & calyculin; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-sPX(I/L/M/V)(phosphorylation)
Curated Info

30

(2010) CST Curation Set: 9640; Year: 2010; Biosample: Jurkat; Disease: T cell leukemia; Treatment: pervanadate & calyculin; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-sPX(I/L/M/V)(phosphorylation)
Curated Info

31

(2010) CST Curation Set: 9639; Year: 2010; Biosample: Jurkat; Disease: T cell leukemia; Treatment: pervanadate & calyculin; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-sPX(I/L/M/V)(phosphorylation)
Curated Info

32

(2010) CST Curation Set: 9637; Year: 2010; Biosample: Jurkat; Disease: T cell leukemia; Treatment: pervanadate & calyculin; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho(Ser/Thr) ATM/ATR Substrate Antibody (polyAB) Cat#: 3005, PTMScan(R) Phospho-ATM/ATR Substrate Motif (pS/pTQ) Immunoaffinity Beads Cat#: 1980
Curated Info

33

Ge F, et al. (2010) Phosphoproteomic analysis of primary human multiple myeloma cells. J Proteomics 73, 1381-90
20230923   Curated Info

34

(2010) CST Curation Set: 8986; Year: 2010; Biosample: Jurkat; Disease: T cell leukemia; Treatment: pervanadate & calyculin; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho(Ser/Thr) ATM/ATR Substrate Antibody (polyAB) Cat#: 3005, PTMScan(R) Phospho-ATM/ATR Substrate Motif (pS/pTQ) Immunoaffinity Beads Cat#: 1980
Curated Info

35

(2010) CST Curation Set: 8973; Year: 2010; Biosample: Jurkat; Disease: T cell leukemia; Treatment: pervanadate & calyculin; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-tXR(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-Threonine-X-Arginine Antibody Cat#: 2351, PTMScan(R) Phospho-Thr-X-Arg Motif (T*XR) Immunoaffinity Beads Cat#: 1988
Curated Info

36

(2010) CST Curation Set: 8987; Year: 2010; Biosample: Jurkat; Disease: T cell leukemia; Treatment: pervanadate & calyculin; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-tXR(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-Threonine-X-Arginine Antibody Cat#: 2351, PTMScan(R) Phospho-Thr-X-Arg Motif (T*XR) Immunoaffinity Beads Cat#: 1988
Curated Info

37

(2010) CST Curation Set: 8994; Year: 2010; Biosample: Jurkat; Disease: T cell leukemia; Treatment: pervanadate & calyculin; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho(Ser/Thr) ATM/ATR Substrate Antibody (polyAB) Cat#: 3005, PTMScan(R) Phospho-ATM/ATR Substrate Motif (pS/pTQ) Immunoaffinity Beads Cat#: 1980
Curated Info

38

(2010) CST Curation Set: 8970; Year: 2010; Biosample: Jurkat; Disease: T cell leukemia; Treatment: pervanadate & calyculin; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho(Ser/Thr) ATM/ATR Substrate Antibody (polyAB) Cat#: 3005, PTMScan(R) Phospho-ATM/ATR Substrate Motif (pS/pTQ) Immunoaffinity Beads Cat#: 1980
Curated Info

39

(2010) CST Curation Set: 8988; Year: 2010; Biosample: Jurkat; Disease: T cell leukemia; Treatment: pervanadate & calyculin; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-PXsP(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-MAPK/CDK Substrates (PXSP or SPXR/K) (34B2) Rabbit mAb Cat#: 2325, PTMScan(R) Phospho-MAPK/CDK Substrate Motif (PXS*P, S*PXK/R) Immunoaffinity Beads Cat#: 1982
Curated Info

40

(2010) CST Curation Set: 8995; Year: 2010; Biosample: Jurkat; Disease: T cell leukemia; Treatment: pervanadate & calyculin; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-tXR(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-Threonine-X-Arginine Antibody Cat#: 2351, PTMScan(R) Phospho-Thr-X-Arg Motif (T*XR) Immunoaffinity Beads Cat#: 1988
Curated Info

41

Olsen JV, et al. (2010) Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal 3, ra3
20068231   Curated Info

42

(2009) CST Curation Set: 7976; Year: 2009; Biosample: Jurkat; Disease: T cell leukemia; Treatment: pervanadate & calyculin; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho(Ser/Thr) ATM/ATR Substrate Antibody (polyAB) Cat#: 3005, PTMScan(R) Phospho-ATM/ATR Substrate Motif (pS/pTQ) Immunoaffinity Beads Cat#: 1980
Curated Info

43

(2009) CST Curation Set: 7977; Year: 2009; Biosample: Jurkat; Disease: T cell leukemia; Treatment: pervanadate & calyculin; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho(Ser/Thr) ATM/ATR Substrate Antibody (polyAB) Cat#: 3005, PTMScan(R) Phospho-ATM/ATR Substrate Motif (pS/pTQ) Immunoaffinity Beads Cat#: 1980
Curated Info

44

(2009) CST Curation Set: 7978; Year: 2009; Biosample: Jurkat; Disease: T cell leukemia; Treatment: pervanadate & calyculin; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho(Ser/Thr) ATM/ATR Substrate Antibody (polyAB) Cat#: 3005, PTMScan(R) Phospho-ATM/ATR Substrate Motif (pS/pTQ) Immunoaffinity Beads Cat#: 1980
Curated Info

45

(2009) CST Curation Set: 7648; Year: 2009; Biosample: Jurkat; Disease: T cell leukemia; Treatment: pervanadate & calyculin; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation)
Curated Info

46

Oppermann FS, et al. (2009) Large-scale proteomics analysis of the human kinome. Mol Cell Proteomics 8, 1751-64
19369195   Curated Info

47

(2009) CST Curation Set: 6246; Year: 2009; Biosample: HeLa; Disease: cervical adenocarcinoma; Treatment: UV; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho(Ser/Thr) ATM/ATR Substrate Antibody (polyAB) Cat#: 3005, PTMScan(R) Phospho-ATM/ATR Substrate Motif (pS/pTQ) Immunoaffinity Beads Cat#: 1980
Curated Info

48

(2009) CST Curation Set: 6247; Year: 2009; Biosample: HeLa; Disease: cervical adenocarcinoma; Treatment: UV; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho(Ser/Thr) ATM/ATR Substrate Antibody (polyAB) Cat#: 3005, PTMScan(R) Phospho-ATM/ATR Substrate Motif (pS/pTQ) Immunoaffinity Beads Cat#: 1980
Curated Info

49

(2009) CST Curation Set: 6241; Year: 2009; Biosample: HeLa; Disease: cervical adenocarcinoma; Treatment: nocodazole; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho(Ser/Thr) ATM/ATR Substrate Antibody (polyAB) Cat#: 3005, PTMScan(R) Phospho-ATM/ATR Substrate Motif (pS/pTQ) Immunoaffinity Beads Cat#: 1980
Curated Info

50

(2009) CST Curation Set: 6240; Year: 2009; Biosample: HeLa; Disease: cervical adenocarcinoma; Treatment: nocodazole; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho(Ser/Thr) ATM/ATR Substrate Antibody (polyAB) Cat#: 3005, PTMScan(R) Phospho-ATM/ATR Substrate Motif (pS/pTQ) Immunoaffinity Beads Cat#: 1980
Curated Info

51

(2009) CST Curation Set: 6049; Year: 2009; Biosample: Jurkat; Disease: T cell leukemia; Treatment: TPA; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(F/Y/M)X(s/t)(L/I/M)(phosphorylation)
Curated Info

52

(2009) CST Curation Set: 6050; Year: 2009; Biosample: Jurkat; Disease: T cell leukemia; Treatment: TPA; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(F/Y/M)X(s/t)(L/I/M)(phosphorylation)
Curated Info

53

(2009) CST Curation Set: 6048; Year: 2009; Biosample: Jurkat; Disease: T cell leukemia; Treatment: untreated; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(F/Y/M)X(s/t)(L/I/M)(phosphorylation)
Curated Info

54

(2009) CST Curation Set: 6047; Year: 2009; Biosample: Jurkat; Disease: T cell leukemia; Treatment: untreated; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(F/Y/M)X(s/t)(L/I/M)(phosphorylation)
Curated Info

55

(2008) CST Curation Set: 5534; Year: 2008; Biosample: MGH lung 14T; Disease: non-small cell lung cancer; Treatment: untreated; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

56

(2008) CST Curation Set: 5535; Year: 2008; Biosample: MGH lung 14N; Disease: non-small cell lung cancer; Treatment: untreated; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

57

(2008) CST Curation Set: 5568; Year: 2008; Biosample: MKN-45; Disease: gastric carcinoma; Treatment: Su11274; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho(Ser/Thr) ATM/ATR Substrate Antibody (polyAB) Cat#: 3005, PTMScan(R) Phospho-ATM/ATR Substrate Motif (pS/pTQ) Immunoaffinity Beads Cat#: 1980
Curated Info

58

(2008) CST Curation Set: 5569; Year: 2008; Biosample: MKN-45; Disease: gastric carcinoma; Treatment: Su11274; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho(Ser/Thr) ATM/ATR Substrate Antibody (polyAB) Cat#: 3005, PTMScan(R) Phospho-ATM/ATR Substrate Motif (pS/pTQ) Immunoaffinity Beads Cat#: 1980
Curated Info

59

Daub H, et al. (2008) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle. Mol Cell 31, 438-48
18691976   Curated Info

60

Dephoure N, et al. (2008) A quantitative atlas of mitotic phosphorylation. Proc Natl Acad Sci U S A 105, 10762-7
18669648   Curated Info

61

(2008) CST Curation Set: 4842; Year: 2008; Biosample: HeLa; Disease: cervical adenocarcinoma; Treatment: UV; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-ATM/ATR Substrate Motif (pS/pTQG) Antibody Cat#: 5074, PTMScan(R) Phospho-ATM/ATR Substrate Motif (pS/pTQG) Immunoaffinity Beads Cat#: 1993
Curated Info

62

(2008) CST Curation Set: 4778; Year: 2008; Biosample: HeLa; Disease: cervical adenocarcinoma; Treatment: UV; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-ATM/ATR Substrate Motif (pS/pTQG) Antibody Cat#: 5074, PTMScan(R) Phospho-ATM/ATR Substrate Motif (pS/pTQG) Immunoaffinity Beads Cat#: 1993
Curated Info

63

(2008) CST Curation Set: 4706; Year: 2008; Biosample: HeLa; Disease: cervical adenocarcinoma; Treatment: untreated; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho(Ser/Thr) ATM/ATR Substrate Antibody (polyAB) Cat#: 3005, PTMScan(R) Phospho-ATM/ATR Substrate Motif (pS/pTQ) Immunoaffinity Beads Cat#: 1980
Curated Info

64

(2008) CST Curation Set: 4707; Year: 2008; Biosample: HeLa; Disease: cervical adenocarcinoma; Treatment: UV; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho(Ser/Thr) ATM/ATR Substrate Antibody (polyAB) Cat#: 3005, PTMScan(R) Phospho-ATM/ATR Substrate Motif (pS/pTQ) Immunoaffinity Beads Cat#: 1980
Curated Info

65

(2008) CST Curation Set: 4390; Year: 2008; Biosample: K562; Disease: chronic myelogenous leukemia; Treatment: untreated; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-[sty](phosphorylation)
Curated Info

66

McSorley T, et al. (2008) Mimicking phosphorylation of Ser-74 on human deoxycytidine kinase selectively increases catalytic activity for dC and dC analogues. FEBS Lett 582, 720-4
18258203   Curated Info

67

(2008) CST Curation Set: 3873; Year: 2008; Biosample: Jurkat; Disease: T cell leukemia; Treatment: pervanadate & calyculin; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation)
Curated Info

68

(2008) CST Curation Set: 3884; Year: 2008; Biosample: Jurkat; Disease: T cell leukemia; Treatment: pervanadate; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-[sty](phosphorylation)
Curated Info

69

(2007) CST Curation Set: 3235; Year: 2007; Biosample: Jurkat; Disease: T cell leukemia; Treatment: pervanadate & calyculin; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho(Ser/Thr) ATM/ATR Substrate Antibody (polyAB) Cat#: 3005, PTMScan(R) Phospho-ATM/ATR Substrate Motif (pS/pTQ) Immunoaffinity Beads Cat#: 1980
Curated Info

70

Smal C, et al. (2007) Positive regulation of deoxycytidine kinase activity by phosphorylation of Ser-74 in B-cell chronic lymphocytic leukaemia lymphocytes. Cancer Lett 253, 68-73
17350163   Curated Info

71

Matsuoka S, et al. (2007) ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage. Science 316, 1160-6
17525332   Curated Info

72

(2007) CST Curation Set: 2796; Year: 2007; Biosample: Jurkat; Disease: T cell leukemia; Treatment: pervanadate & calyculin; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-ATM/ATR Substrate Motif (pS/pTQG) Antibody Cat#: 5074, PTMScan(R) Phospho-ATM/ATR Substrate Motif (pS/pTQG) Immunoaffinity Beads Cat#: 1993
Curated Info

73

(2007) CST Curation Set: 2339; Year: 2007; Biosample: Jurkat; Disease: T cell leukemia; Treatment: calyculin & pervanadate; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-(Ser/Thr) ATM/ATR Substrate Antibody Cat#: 2851
Curated Info

74

(2006) CST Curation Set: 1772; Year: 2006; Biosample: GM00200; Disease: -; Treatment: UV; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-(Ser/Thr) ATM/ATR Substrate Antibody Cat#: 2851
Curated Info

75

(2006) CST Curation Set: 1773; Year: 2006; Biosample: GM00200; Disease: -; Treatment: UV; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-(Ser/Thr) ATM/ATR Substrate Antibody Cat#: 2851
Curated Info

76

(2006) CST Curation Set: 1774; Year: 2006; Biosample: GM18366; Disease: Seckel syndrome; Treatment: UV; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-(Ser/Thr) ATM/ATR Substrate Antibody Cat#: 2851
Curated Info

77

(2006) CST Curation Set: 1775; Year: 2006; Biosample: GM18366; Disease: Seckel syndrome; Treatment: UV; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-(Ser/Thr) ATM/ATR Substrate Antibody Cat#: 2851
Curated Info

78

(2006) CST Curation Set: 1614; Year: 2006; Biosample: M059K; Disease: glioblastoma; Treatment: normal; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-(Ser/Thr) ATM/ATR Substrate Antibody Cat#: 2851
Curated Info

79

(2006) CST Curation Set: 1615; Year: 2006; Biosample: M059K; Disease: glioblastoma; Treatment: normal; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-(Ser/Thr) ATM/ATR Substrate Antibody Cat#: 2851
Curated Info

80

(2006) CST Curation Set: 1616; Year: 2006; Biosample: M059K; Disease: glioblastoma; Treatment: UV; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-(Ser/Thr) ATM/ATR Substrate Antibody Cat#: 2851
Curated Info

81

(2006) CST Curation Set: 1617; Year: 2006; Biosample: M059K; Disease: glioblastoma; Treatment: UV; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-(Ser/Thr) ATM/ATR Substrate Antibody Cat#: 2851
Curated Info

82

Smal C, et al. (2006) Identification of in vivo phosphorylation sites on human deoxycytidine kinase. Role of Ser-74 in the control of enzyme activity. J Biol Chem 281, 4887-93
16361699   Curated Info

83

(2006) CST Curation Set: 1122; Year: 2006; Biosample: M059K; Disease: glioblastoma; Treatment: UV; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-(Ser/Thr) ATM/ATR Substrate Antibody Cat#: 2851
Curated Info

84

(2006) CST Curation Set: 1123; Year: 2006; Biosample: M059K; Disease: glioblastoma; Treatment: UV; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-(Ser/Thr) ATM/ATR Substrate Antibody Cat#: 2851
Curated Info

85

(2006) CST Curation Set: 1091; Year: 2006; Biosample: M059K; Disease: glioblastoma; Treatment: UV; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-(Ser/Thr) ATM/ATR Substrate Antibody Cat#: 2851
Curated Info

86

(2006) CST Curation Set: 1092; Year: 2006; Biosample: M059J; Disease: glioblastoma; Treatment: UV; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-(Ser/Thr) ATM/ATR Substrate Antibody Cat#: 2851
Curated Info

87

(2006) CST Curation Set: 1093; Year: 2006; Biosample: M059K; Disease: glioblastoma; Treatment: UV; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-[st](phosphorylation)
Curated Info

88

(2006) CST Curation Set: 1094; Year: 2006; Biosample: M059J; Disease: glioblastoma; Treatment: UV; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-[st](phosphorylation)
Curated Info

89

Smal C, et al. (2006) Identification of phosphorylation sites on human deoxycytidine kinase after overexpression in eucaryotic cells. Nucleosides Nucleotides Nucleic Acids 25, 1141-6
17065079   Curated Info

90

(2005) CST Curation Set: 994; Year: 2005; SILAC: Y; Biosample1/Treatment/Isotope: M059K/UV/L; Biosample2/Treatment/Isotope: M059K/UV/H; Disease: glioblastoma; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-(Ser/Thr) ATM/ATR Substrate Antibody Cat#: 2851
Curated Info

91

(2005) CST Curation Set: 995; Year: 2005; SILAC: Y; Biosample1/Treatment/Isotope: M059K/UV/L; Biosample2/Treatment/Isotope: M059K/UV/H; Disease: glioblastoma; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-(Ser/Thr) ATM/ATR Substrate Antibody Cat#: 2851
Curated Info

92

(2005) CST Curation Set: 996; Year: 2005; SILAC: Y; Biosample1/Treatment/Isotope: M059K/UV/L; Biosample2/Treatment/Isotope: M059K/UV/H; Disease: glioblastoma; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-(Ser/Thr) ATM/ATR Substrate Antibody Cat#: 2851
Curated Info

93

(2005) CST Curation Set: 909; Year: 2005; Biosample: M059K; Disease: glioblastoma; Treatment: UV; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-(Ser/Thr) ATM/ATR Substrate Antibody Cat#: 2851
Curated Info

94

(2005) CST Curation Set: 908; Year: 2005; Biosample: M059J; Disease: glioblastoma; Treatment: UV; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-(Ser/Thr) ATM/ATR Substrate Antibody Cat#: 2851
Curated Info

95

(2005) CST Curation Set: 911; Year: 2005; Biosample: M059K; Disease: glioblastoma; Treatment: UV; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-[st](phosphorylation)
Curated Info

96

(2004) CST Curation Set: 432; Year: 2004; Biosample: 293T; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-(Ser/Thr) ATM/ATR Substrate Antibody Cat#: 2851
Curated Info

97

(2004) CST Curation Set: 433; Year: 2004; Biosample: 293T; Disease: -; Treatment: NCS; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-(Ser/Thr) ATM/ATR Substrate Antibody Cat#: 2851
Curated Info

98

(2004) CST Curation Set: 309; Year: 2004; Biosample: M059K; Disease: glioblastoma; Treatment: UV; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-(Ser/Thr) ATM/ATR Substrate Antibody Cat#: 2851
Curated Info

99

(2004) CST Curation Set: 310; Year: 2004; Biosample: M059K; Disease: glioblastoma; Treatment: UV; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-(Ser/Thr) ATM/ATR Substrate Antibody Cat#: 2851
Curated Info

100

(2004) CST Curation Set: 311; Year: 2004; Biosample: M059K; Disease: glioblastoma; Treatment: etoposide; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-(Ser/Thr) ATM/ATR Substrate Antibody Cat#: 2851
Curated Info

101

(2004) CST Curation Set: 312; Year: 2004; Biosample: M059K; Disease: glioblastoma; Treatment: etoposide; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)Q(phosphorylation) Antibody Used to Purify Peptides prior to MS2: Phospho-(Ser/Thr) ATM/ATR Substrate Antibody Cat#: 2851
Curated Info

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