Lys52
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Ubiquitination Site Page:
Lys52 - ACTA2 (mouse)

Site Information
VMVGMGQkDSYVGDE    SwissProt Entrez-Gene
Predicted information: Scansite
Orthologous residues: ACTA2 (human): K52
Blast this site against: NCBI  SwissProt  PDB 

In vivo Characterization
Methods used to characterize site in vivo: mass spectrometry (1, 8, 9, 10)
Relevant cell line - cell type - tissue: 'brain, embryonic' (9), brain (1, 10), heart (1), kidney (1), liver (1), muscle (1), pooled tissues (1)




References

1

Wagner SA, et al. (2012) Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues. Mol Cell Proteomics 11, 1578-85
22790023   Curated Info

2

(2010) CST Curation Set: 9134; Year: 2010; SILAC: N; Biosample/Treatment: PY2(cell line)/untreated; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK
Curated Info

3

(2010) CST Curation Set: 9086; Year: 2010; SILAC: N; Biosample/Treatment: embryonic stem cells (mouse)(cell line)/untreated; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK
Curated Info

4

(2009) CST Curation Set: 8159; Year: 2009; SILAC: N; Biosample/Treatment: spleen(tissue)/untreated; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK Antibody Used to Purify Peptides prior to MS2: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990
Curated Info

5

(2009) CST Curation Set: 8160; Year: 2009; SILAC: N; Biosample/Treatment: testis(tissue)/untreated; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK Antibody Used to Purify Peptides prior to MS2: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990
Curated Info

6

(2009) CST Curation Set: 8161; Year: 2009; SILAC: N; Biosample/Treatment: thymus(tissue)/untreated; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK Antibody Used to Purify Peptides prior to MS2: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990
Curated Info

7

(2009) CST Curation Set: 8158; Year: 2009; SILAC: N; Biosample/Treatment: muscle(tissue)/untreated; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK Antibody Used to Purify Peptides prior to MS2: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990
Curated Info

8

(2008) CST Curation Set: 4126; Year: 2008; SILAC: N; Biosample/Treatment: brain(tissue)/untreated; Disease: Alzheimer's disease; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK
Curated Info

9

(2007) CST Curation Set: 3116; Year: 2007; SILAC: N; Biosample/Treatment: brain, embryonic(tissue)/-; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK
Curated Info

10

(2007) CST Curation Set: 3117; Year: 2007; SILAC: N; Biosample/Treatment: brain(tissue)/-; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK
Curated Info

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