Ser431
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Phosphorylation Site Page:
Ser431 - abLIM (human)

Site Information
GESPRTLsPTPSAEG    SwissProt Entrez-Gene
Predicted information: Scansite
Orthologous residues: abLIM (mouse): S475, abLIM (rat): S115, abLIM iso3 (mouse): S355, abLIM iso2 (mouse): S355, abLIM iso5 (human): S143
Blast this site against: NCBI  SwissProt  PDB 

In vivo Characterization
Methods used to characterize site in vivo: mass spectrometry (2, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
Disease tissue studied: bone cancer (7), osteosarcoma (7), cervical cancer (8), cervical adenocarcinoma (8), hepatocellular carcinoma, surrounding tissue (6)
Relevant cell line - cell type - tissue: 'muscle, skeletal' (9), 'stem, embryonic' (11), 293 (epithelial) [AT1 (human), transfection, AT1R stable transfected HEK293] (5), DG75 (B lymphocyte) (4), HeLa (cervical) (13, 17), HeLa S3 (cervical) (8), hepatocyte-liver (6), HUES-7 ('stem, embryonic') (10), Jurkat (T lymphocyte) (2, 12, 15, 16, 18), leukocyte-blood (7), platelet-blood (14), U2OS (bone cell) (7)

Controlled by
Treatments: anti-CD3 (12), nocodazole (8)



References

1

Weber C, Schreiber TB, Daub H (2012) Dual phosphoproteomics and chemical proteomics analysis of erlotinib and gefitinib interference in acute myeloid leukemia cells. J Proteomics 75, 1343-56
22115753   Curated Info

2

(2011) CST Curation Set: 11892; Year: 2011; Biosample: Jurkat; Disease: T cell leukemia; Treatment: calyculin & pervanadate; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-[s/t/y](phosphorylation)
Curated Info

3

Rigbolt KT, et al. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation. Sci Signal 4, rs3
21406692   Curated Info

4

Iliuk AB, et al. (2010) In-depth analyses of kinase-dependent tyrosine phosphoproteomes based on metal ion-functionalized soluble nanopolymers. Mol Cell Proteomics 9, 2162-72
20562096   Curated Info

5

Christensen GL, et al. (2010) Quantitative phosphoproteomics dissection of seven-transmembrane receptor signaling using full and biased agonists. Mol Cell Proteomics 9, 1540-53
20363803   Curated Info

6

Han G, et al. (2010) Phosphoproteome analysis of human liver tissue by long-gradient nanoflow LC coupled with multiple stage MS analysis. Electrophoresis 31, 1080-9
20166139   Curated Info

7

Raijmakers R, et al. (2010) Exploring the human leukocyte phosphoproteome using a microfluidic reversed-phase-TiO2-reversed-phase high-performance liquid chromatography phosphochip coupled to a quadrupole time-of-flight mass spectrometer. Anal Chem 82, 824-32
20058876   Curated Info

8

Olsen JV, et al. (2010) Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal 3, ra3
20068231   Curated Info

9

Højlund K, et al. (2009) In vivo phosphoproteome of human skeletal muscle revealed by phosphopeptide enrichment and HPLC-ESI-MS/MS. J Proteome Res 8, 4954-65
19764811   Curated Info

10

Van Hoof D, et al. (2009) Phosphorylation dynamics during early differentiation of human embryonic stem cells. Cell Stem Cell 5, 214-26
19664995   Curated Info

11

Brill LM, et al. (2009) Phosphoproteomic analysis of human embryonic stem cells. Cell Stem Cell 5, 204-13
19664994   Curated Info

12

Mayya V, et al. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions. Sci Signal 2, ra46
19690332   Curated Info

13

Dephoure N, et al. (2008) A quantitative atlas of mitotic phosphorylation. Proc Natl Acad Sci U S A 105, 10762-7
18669648   Curated Info

14

Zahedi RP, et al. (2008) Phosphoproteome of resting human platelets. J Proteome Res 7, 526-34
18088087   Curated Info

15

(2007) CST Curation Set: 2935; Year: 2007; Biosample: Jurkat; Disease: T cell leukemia; Treatment: pervanadate; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

16

Ficarro SB, et al. (2005) Automated immobilized metal affinity chromatography/nano-liquid chromatography/electrospray ionization mass spectrometry platform for profiling protein phosphorylation sites. Rapid Commun Mass Spectrom 19, 57-71
15570572   Curated Info

17

Beausoleil SA, et al. (2004) Large-scale characterization of HeLa cell nuclear phosphoproteins. Proc Natl Acad Sci U S A 101, 12130-5
15302935   Curated Info

18

Brill LM, et al. (2004) Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry. Anal Chem 76, 2763-72
15144186   Curated Info

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