Lys238

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Ubiquitination Site Page:

Lys238 - IkB-alpha (human)

Site Information

DLVSLLLkCGADVNR SwissProt Entrez-Gene

Predicted information: Scansite

Orthologous residues: IkB‑alpha (mouse): K238, IkB‑alpha (rat): K238

Blast this site against: NCBI SwissProt PDB

In vivo Characterization

Methods used to characterize site in vivo:	mass spectrometry (1, 7, 8)
Relevant cell line - cell type - tissue:	U266 (B lymphocyte) (7)

	References

1	Kim W, et al. (2011) Systematic and quantitative assessment of the ubiquitin-modified proteome. Mol Cell 44, 325-40 21906983 Curated Info
2	(2010) CST Curation Set: 10367; Year: 2010; Biosample: L363; Disease: multiple myeloma; Treatment: untreated; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK Antibody Used to Purify Peptides prior to MS2: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990 Curated Info
3	(2010) CST Curation Set: 10366; Year: 2010; Biosample: L363; Disease: multiple myeloma; Treatment: untreated; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK Antibody Used to Purify Peptides prior to MS2: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990 Curated Info
4	(2010) CST Curation Set: 10260; Year: 2010; Biosample: L363; Disease: multiple myeloma; Treatment: untreated; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK Antibody Used to Purify Peptides prior to MS2: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990 Curated Info
5	(2010) CST Curation Set: 10261; Year: 2010; Biosample: L363; Disease: multiple myeloma; Treatment: untreated; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK Antibody Used to Purify Peptides prior to MS2: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990 Curated Info
6	(2010) CST Curation Set: 8771; Year: 2010; Biosample: AMO-1; Disease: multiple myeloma; Treatment: Velcade; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK Antibody Used to Purify Peptides prior to MS2: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990 Curated Info
7	(2009) CST Curation Set: 8645; Year: 2009; Biosample: U266; Disease: multiple myeloma; Treatment: Velcade; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK Antibody Used to Purify Peptides prior to MS2: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990 Curated Info
8	(2009) CST Curation Set: 8633; Year: 2009; Biosample: AMO-1; Disease: multiple myeloma; Treatment: Velcade; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK Antibody Used to Purify Peptides prior to MS2: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990 Curated Info

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