Lys50
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Ubiquitination Site Page:
Lys50 - GSTM1 (human)

Site Information
RSQWLNEkFKLGLDF    SwissProt Entrez-Gene
Predicted information: Scansite
Orthologous residues: GSTM1 (mouse): K50, GSTM1 iso2 (human): K50, GSTM1 (rat): K50
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 10839285

In vivo Characterization
Methods used to characterize site in vivo: mass spectrometry (1, 4, 5, 6)
Disease tissue studied: multiple myeloma (6)
Relevant cell line - cell type - tissue: U266 (B lymphocyte) (4, 5, 6)




References

1

Kim W, et al. (2011) Systematic and quantitative assessment of the ubiquitin-modified proteome. Mol Cell 44, 325-40
21906983   Curated Info

2

(2010) CST Curation Set: 10264; Year: 2010; Biosample/Treatment: cell line, U266/untreated; Disease: -; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-K-e-GG Antibody Used to Purify Peptides prior to MS2: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990
Curated Info

3

(2010) CST Curation Set: 8771; Year: 2009; Biosample/Treatment: cell line, AMO-1/Velcade; Disease: -; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-K-e-GG Antibody Used to Purify Peptides prior to MS2: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990
Curated Info

4

(2009) CST Curation Set: 8646; Year: 2009; Biosample/Treatment: cell line, U266/Velcade; Disease: -; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-K-e-GG Antibody Used to Purify Peptides prior to MS2: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990
Curated Info

5

(2009) CST Curation Set: 8645; Year: 2009; Biosample/Treatment: cell line, U266/Velcade; Disease: -; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-K-e-GG Antibody Used to Purify Peptides prior to MS2: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990
Curated Info

6

(2009) CST Curation Set: 6588; Year: 2009; Biosample/Treatment: cell line, U266/MG132; Disease: -; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-K-e-GG
Curated Info

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