Lys95
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Ubiquitination Site Page:
Lys95 - LAGE3 (human)

Site Information
PHQRVVGkDLTVSGR    SwissProt Entrez-Gene
Predicted information: Scansite
Orthologous residues: LAGE3 (mouse): K100
Blast this site against: NCBI  SwissProt  PDB 

In vivo Characterization
Methods used to characterize site in vivo: mass spectrometry (1, 2, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45)
Disease tissue studied: multiple myeloma (18)
Relevant cell line - cell type - tissue: 293T (epithelial) (2), KMS-12-BM (B lymphocyte) (18), KMS-27 (B lymphocyte) (14, 17, 19, 20), LNCaP (prostate cell) (41, 42, 43, 44), MV4-11 (macrophage) (2), NCI-H929 (B lymphocyte) (8, 9), PC3 (prostate cell) (34, 35, 36, 37, 38, 39, 40), RPMI-8266 (B lymphocyte) (10, 11), U266 (B lymphocyte) (21, 22, 23, 25, 26, 27, 45)




References

1

Kim W, et al. (2011) Systematic and quantitative assessment of the ubiquitin-modified proteome. Mol Cell 44, 325-40
21906983   Curated Info

2

Wagner SA, et al. (2011) A Proteome-wide, Quantitative Survey of In Vivo Ubiquitylation Sites Reveals Widespread Regulatory Roles. Mol Cell Proteomics 10, M111.013284
21890473   Curated Info

3

(2010) CST Curation Set: 10367; Year: 2010; SILAC: N; Biosample/Treatment: L363(cell line)/untreated; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK Antibody Used to Purify Peptides prior to MS2: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990
Curated Info

4

(2010) CST Curation Set: 10261; Year: 2010; SILAC: N; Biosample/Treatment: L363(cell line)/untreated; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK Antibody Used to Purify Peptides prior to MS2: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990
Curated Info

5

(2010) CST Curation Set: 9192; Year: 2010; SILAC: N; Biosample/Treatment: U266(cell line)/untreated; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK Antibody Used to Purify Peptides prior to MS2: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990
Curated Info

6

(2009) CST Curation Set: 8679; Year: 2009; SILAC: N; Biosample/Treatment: Karpas-620(cell line)/untreated; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK Antibody Used to Purify Peptides prior to MS2: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990
Curated Info

7

(2009) CST Curation Set: 8682; Year: 2009; SILAC: N; Biosample/Treatment: Karpas-620(cell line)/Velcade; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK Antibody Used to Purify Peptides prior to MS2: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990
Curated Info

8

(2009) CST Curation Set: 8675; Year: 2009; SILAC: N; Biosample/Treatment: NCI-H929(cell line)/Velcade; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK Antibody Used to Purify Peptides prior to MS2: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990
Curated Info

9

(2009) CST Curation Set: 8674; Year: 2009; SILAC: N; Biosample/Treatment: NCI-H929(cell line)/untreated; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK Antibody Used to Purify Peptides prior to MS2: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990
Curated Info

10

(2009) CST Curation Set: 8668; Year: 2009; SILAC: N; Biosample/Treatment: RPMI-8266(cell line)/Velcade; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK Antibody Used to Purify Peptides prior to MS2: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990
Curated Info

11

(2009) CST Curation Set: 8667; Year: 2009; SILAC: N; Biosample/Treatment: RPMI-8266(cell line)/Velcade; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK Antibody Used to Purify Peptides prior to MS2: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990
Curated Info

12

(2009) CST Curation Set: 8657; Year: 2009; SILAC: N; Biosample/Treatment: KMS-12-BM(cell line)/Velcade; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK Antibody Used to Purify Peptides prior to MS2: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990
Curated Info

13

(2009) CST Curation Set: 8656; Year: 2009; SILAC: N; Biosample/Treatment: KMS-12-BM(cell line)/Velcade; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK Antibody Used to Purify Peptides prior to MS2: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990
Curated Info

14

(2009) CST Curation Set: 8659; Year: 2009; SILAC: N; Biosample/Treatment: KMS-27(cell line)/untreated; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK Antibody Used to Purify Peptides prior to MS2: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990
Curated Info

15

(2009) CST Curation Set: 8654; Year: 2009; SILAC: N; Biosample/Treatment: KMS-12-BM(cell line)/untreated; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK Antibody Used to Purify Peptides prior to MS2: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990
Curated Info

16

(2009) CST Curation Set: 8655; Year: 2009; SILAC: N; Biosample/Treatment: KMS-12-BM(cell line)/Velcade; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK Antibody Used to Purify Peptides prior to MS2: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990
Curated Info

17

(2009) CST Curation Set: 8664; Year: 2009; SILAC: N; Biosample/Treatment: KMS-27(cell line)/Velcade; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK Antibody Used to Purify Peptides prior to MS2: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990
Curated Info

18

(2009) CST Curation Set: 8658; Year: 2009; SILAC: N; Biosample/Treatment: KMS-12-BM(cell line)/Velcade; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK Antibody Used to Purify Peptides prior to MS2: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990
Curated Info

19

(2009) CST Curation Set: 8660; Year: 2009; SILAC: N; Biosample/Treatment: KMS-27(cell line)/untreated; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK Antibody Used to Purify Peptides prior to MS2: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990
Curated Info

20

(2009) CST Curation Set: 8662; Year: 2009; SILAC: N; Biosample/Treatment: KMS-27(cell line)/Velcade; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK Antibody Used to Purify Peptides prior to MS2: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990
Curated Info

21

(2009) CST Curation Set: 8646; Year: 2009; SILAC: N; Biosample/Treatment: U266(cell line)/Velcade; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK Antibody Used to Purify Peptides prior to MS2: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990
Curated Info

22

(2009) CST Curation Set: 8643; Year: 2009; SILAC: N; Biosample/Treatment: U266(cell line)/Velcade; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK Antibody Used to Purify Peptides prior to MS2: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990
Curated Info

23

(2009) CST Curation Set: 8642; Year: 2009; SILAC: N; Biosample/Treatment: U266(cell line)/untreated; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK Antibody Used to Purify Peptides prior to MS2: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990
Curated Info

24

(2009) CST Curation Set: 8653; Year: 2009; SILAC: N; Biosample/Treatment: KMS-12-BM(cell line)/untreated; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK Antibody Used to Purify Peptides prior to MS2: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990
Curated Info

25

(2009) CST Curation Set: 8645; Year: 2009; SILAC: N; Biosample/Treatment: U266(cell line)/Velcade; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK Antibody Used to Purify Peptides prior to MS2: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990
Curated Info

26

(2009) CST Curation Set: 8641; Year: 2009; SILAC: N; Biosample/Treatment: U266(cell line)/untreated; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK Antibody Used to Purify Peptides prior to MS2: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990
Curated Info

27

(2009) CST Curation Set: 8644; Year: 2009; SILAC: N; Biosample/Treatment: U266(cell line)/Velcade; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK Antibody Used to Purify Peptides prior to MS2: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990
Curated Info

28

(2009) CST Curation Set: 8639; Year: 2009; SILAC: N; Biosample/Treatment: L363(cell line)/Velcade; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK Antibody Used to Purify Peptides prior to MS2: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990
Curated Info

29

(2009) CST Curation Set: 8635; Year: 2009; SILAC: N; Biosample/Treatment: L363(cell line)/untreated; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK Antibody Used to Purify Peptides prior to MS2: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990
Curated Info

30

(2009) CST Curation Set: 8637; Year: 2009; SILAC: N; Biosample/Treatment: L363(cell line)/Velcade; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK Antibody Used to Purify Peptides prior to MS2: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990
Curated Info

31

(2009) CST Curation Set: 8640; Year: 2009; SILAC: N; Biosample/Treatment: L363(cell line)/Velcade; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK Antibody Used to Purify Peptides prior to MS2: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990
Curated Info

32

(2009) CST Curation Set: 8638; Year: 2009; SILAC: N; Biosample/Treatment: L363(cell line)/Velcade; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK Antibody Used to Purify Peptides prior to MS2: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990
Curated Info

33

(2009) CST Curation Set: 8636; Year: 2009; SILAC: N; Biosample/Treatment: L363(cell line)/untreated; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK Antibody Used to Purify Peptides prior to MS2: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990
Curated Info

34

(2009) CST Curation Set: 8587; Year: 2010; SILAC: N; Biosample/Treatment: PC3(cell line)/Velcade; Disease: prostate cancer; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK
Curated Info

35

(2009) CST Curation Set: 8585; Year: 2010; SILAC: N; Biosample/Treatment: PC3(cell line)/Geldanamycin; Disease: prostate cancer; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK
Curated Info

36

(2009) CST Curation Set: 8586; Year: 2010; SILAC: N; Biosample/Treatment: PC3(cell line)/Velcade; Disease: prostate cancer; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK
Curated Info

37

(2009) CST Curation Set: 8583; Year: 2010; SILAC: N; Biosample/Treatment: PC3(cell line)/AAG; Disease: prostate cancer; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK
Curated Info

38

(2009) CST Curation Set: 8581; Year: 2010; SILAC: N; Biosample/Treatment: PC3(cell line)/control; Disease: prostate cancer; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK
Curated Info

39

(2009) CST Curation Set: 8582; Year: 2010; SILAC: N; Biosample/Treatment: PC3(cell line)/AAG; Disease: prostate cancer; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK
Curated Info

40

(2009) CST Curation Set: 8584; Year: 2010; SILAC: N; Biosample/Treatment: PC3(cell line)/Geldanamycin; Disease: prostate cancer; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK
Curated Info

41

(2009) CST Curation Set: 8244; Year: 2010; SILAC: N; Biosample/Treatment: LNCaP(cell line)/Geldanamycin; Disease: prostate cancer; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK
Curated Info

42

(2009) CST Curation Set: 8242; Year: 2010; SILAC: N; Biosample/Treatment: LNCaP(cell line)/AAG; Disease: prostate cancer; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK
Curated Info

43

(2009) CST Curation Set: 8246; Year: 2010; SILAC: N; Biosample/Treatment: LNCaP(cell line)/Velcade; Disease: prostate cancer; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK
Curated Info

44

(2009) CST Curation Set: 8245; Year: 2010; SILAC: N; Biosample/Treatment: LNCaP(cell line)/Geldanamycin; Disease: prostate cancer; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK
Curated Info

45

(2009) CST Curation Set: 6586; Year: 2009; SILAC: N; Biosample/Treatment: U266(cell line)/control; Disease: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-UbK
Curated Info

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