Tyr1178
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Phosphorylation Site Page:
Tyr1178 - InsR iso2 (human)

Site Information
DIYETDYyRKGGKGL    SwissProt Entrez-Gene
Predicted information: Scansite
Orthologous residues: InsR (mouse): Y1180, InsR (human): Y1190, InsR (rat): Y1191
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447682

In vivo Characterization
Methods used to characterize site in vivo: [32P] bio-synthetic labeling (4), mass spectrometry (1, 2, 3), peptide sequencing (4), phospho-antibody (4), phosphoamino acid analysis (4)
Disease tissue studied: colorectal cancer (3), colorectal carcinoma (3)
Relevant cell line - cell type - tissue: 3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout] (4), CHO (fibroblast) [EphB1 (human), transfection] (4), HT-29 (intestinal) (3), NCI-H2228 (pulmonary) (1, 2)

Controlled by
Kinases, in vitro: InsR (human) (4)
Treatments: insulin (4)



References

1

(2008) CST Curation Set: 5327; Year: 2008; Biosample/Treatment: cell line, NCI-H2228/untreated; Disease: non-small cell lung adenocarcinoma; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

2

(2008) CST Curation Set: 5328; Year: 2008; Biosample/Treatment: cell line, NCI-H2228/untreated; Disease: non-small cell lung adenocarcinoma; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

3

(2004) CST Curation Set: 370; Year: 2004; Biosample/Treatment: cell line, HT-29/Serum starved & IGF; Disease: colorectal carcinoma; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

4

Tennagels N, et al. (2001) Identification of Ser(1275) and Ser(1309) as autophosphorylation sites of the human insulin receptor in intact cells. Biochem Biophys Res Commun 282, 387-93
11401470   Curated Info

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