Tyr1440
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Phosphorylation Site Page:
Tyr1440 - ITGB4 iso2 (human)

Site Information
STTLPRDySTLTSVS   SwissProt Entrez-Gene
Predicted information:  Scansite
Orthologous residues: ITGB4 (human): Y1510, ITGB4 iso3 (mouse): (Y1441), ITGB4 (mouse): (Y1506), ITGB4 iso3 (human): Y1440, ITGB4 iso2 (mouse): (Y1441), ITGB4 (rat): (Y1441)
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 450141

In vivo Characterization
Methods used to characterize site in vivo: immunoprecipitation (1), mass spectrometry (2, 3, 4, 5), mutation of modification site (1, 6), phospho-antibody (1, 6), western blotting (1, 6)
Disease tissue studied: bladder cancer (6), breast cancer (1), pancreatic cancer (5)
Relevant cell line - cell type - tissue: 293T (epithelial) (6), 804G (epithelial) (6), BxPC-3 (pancreatic) (5), HaCaT (keratinocyte) (6), HUVEC (endothelial) (6), MDA-MB231 (breast cell) (1), MDA-MB435 (breast cell) (1), MKN-45 (gastric) (2, 3), NCI-H2228 (pulmonary) (4)

Controlled by
Treatments: vanadate (6)

Downstream Regulation
Effects of modification on ITGB4 iso2: activity, induced (1), molecular association, regulation (6)
Effects of modification on biological processes: cell adhesion, altered (6), cell motility, altered (1)
Induce interaction with: SHC1 (human) (6)


References

1

Yang X, Dutta U, Shaw LM (2010) SHP2 mediates the localized activation of Fyn downstream of the α6β4 integrin to promote carcinoma invasion. Mol Cell Biol 30, 5306-17
20855525   Curated Info

2

(2008) CST Curation Set: 5770; Year: 2008; Biosample/Treatment: cell line, MKN-45/Su11274; Disease: gastric carcinoma; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

3

(2008) CST Curation Set: 5771; Year: 2008; Biosample/Treatment: cell line, MKN-45/Su11274; Disease: gastric carcinoma; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

4

(2008) CST Curation Set: 5327; Year: 2008; Biosample/Treatment: cell line, NCI-H2228/untreated; Disease: non-small cell lung adenocarcinoma; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

5

(2004) CST Curation Set: 411; Year: 2004; Biosample1/Treatment/Isotope: cell line, BxPC-3/EGF & Iressa/L, Biosample2/Treatment/Isotope: cell line, BxPC-3/EGF/H; Disease: pancreatic carcinoma; SILAC: Y; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

6

Dans M, et al. (2001) Tyrosine phosphorylation of the beta 4 integrin cytoplasmic domain mediates Shc signaling to extracellular signal-regulated kinase and antagonizes formation of hemidesmosomes. J Biol Chem 276, 1494-502
11044453   Curated Info

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