Phosphorylation Site Page:
In vivo Characterization
Methods used to characterize site
mass spectrometry ( 2, 3, 4, 5, 6)
Disease tissue studied:
brain cancer ( 4), neuroendocrine cancer ( 4)
Relevant cell line - cell type - tissue:
32Dcl3 (myeloid) [FLT3 (mouse), transfection, chimera with human FLT3-ITD mutant (corresponding to wild type P36888 ~aa 525-695 ETILLNS...IFEYCC)] ( 6), brain ( 4), liver ( 3), liver [leptin (mouse), homozygous knockout] ( 3), macrophage-peritoneum ( 2), macrophage-peritoneum [RIP3 (mouse), homozygous knockout] ( 2), MEF (fibroblast) [p53 (mouse), homozygous knockout] ( 5)
RIP3 (mouse) ( 2)
Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels.
Mol Cell Proteomics 13, 1690-704
Wu X, et al. (2012) Investigation of receptor interacting protein (RIP3)-dependent protein phosphorylation by quantitative phosphoproteomics.
Mol Cell Proteomics 11, 1640-51
Grimsrud PA, et al. (2012) A quantitative map of the liver mitochondrial phosphoproteome reveals posttranslational control of ketogenesis.
Cell Metab 16, 672-83
CST Curation Set: 12730; Year: 2011; Biosample/Treatment: tissue, brain/untreated; Disease: brain cancer; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-(s/t)P(phosphorylation)
Hsu PP, et al. (2011) The mTOR-regulated phosphoproteome reveals a mechanism of mTORC1-mediated inhibition of growth factor signaling.
Science 332, 1317-22
Choudhary C, et al. (2009) Mislocalized activation of oncogenic RTKs switches downstream signaling outcomes.
Mol Cell 36, 326-39