Phosphorylation Site Page:
In vivo Characterization
Methods used to characterize site
mutation of modification site ( 1, 3), phospho-antibody ( 1, 3), western blotting ( 1, 3)
Disease tissue studied:
adrenal cancer ( 3), pheochromocytoma ( 3)
Relevant cell line - cell type - tissue:
293T (epithelial) ( 1, 3), MEF (fibroblast) ( 3), PC-12 (chromaffin) ( 3)
Putative upstream kinases:
Abl (human) ( 3)
Abl (human) ( 3), Arg (human) ( 2)
NGF ( 3)
Effects of modification on CRK:
molecular association, regulation ( 1, 3), protein conformation ( 1)
Effects of modification on biological processes:
cell adhesion, altered ( 3), cell differentiation, altered ( 3)
Inhibit interaction with:
Abl (human) ( 1), Abl iso2 (human) ( 3), PXN (chicken) ( 3)
Sarkar P, et al. (2007) Proline cis-trans isomerization controls autoinhibition of a signaling protein.
Mol Cell 25, 413-26
Hu H, Bliss JM, Wang Y, Colicelli J (2005) RIN1 is an ABL tyrosine kinase activator and a regulator of epithelial-cell adhesion and migration.
Curr Biol 15, 815-23
Escalante M, et al. (2000) Phosphorylation of c-Crk II on the negative regulatory Tyr222 mediates nerve growth factor-induced cell spreading and morphogenesis.
J Biol Chem 275, 24787-97