Phosphorylation Site Page:
In vivo Characterization
Methods used to characterize site
mass spectrometry ( 1, 3, 4, 5)
Relevant cell line - cell type - tissue:
'3T3-L1, differentiated' (adipocyte) ( 1, 3), brain ( 4), MEF (fibroblast) [TSC2 (mouse), homozygous knockout] ( 5)
Parker BL, et al. (2015) Targeted phosphoproteomics of insulin signaling using data-independent acquisition mass spectrometry.
Sci Signal 8, rs6
Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels.
Mol Cell Proteomics 13, 1690-704
Humphrey SJ, et al. (2013) Dynamic Adipocyte Phosphoproteome Reveals that Akt Directly Regulates mTORC2.
Cell Metab 17, 1009-20
Trinidad JC, et al. (2012) Global identification and characterization of both O-GlcNAcylation and phosphorylation at the murine synapse.
Mol Cell Proteomics 11, 215-29
Yu Y, et al. (2011) Phosphoproteomic analysis identifies Grb10 as an mTORC1 substrate that negatively regulates insulin signaling.
Science 332, 1322-6