Tyr272
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Home > Phosphorylation Site Page: > Tyr272  -  SHC1 iso3 (human)

Site Information
ELFDDPSyVNVQNLD   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448511
Associated spectra:  6 CST

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 1 , 2 , 3 ) , mutation of modification site ( 4 ) , phospho-antibody ( 4 ) , western blotting ( 4 )
Relevant cell line - cell type - tissue:
3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout] ( 4 ) , HEK293T (epithelial) ( 2 , 3 , 4 ) , Jurkat (T lymphocyte) ( 1 )

Upstream Regulation
Kinases, in vitro:
EGFR (human) ( 5 ) , Src (human) ( 5 )

Downstream Regulation
Effects of modification on SHC1 iso3:
phosphorylation ( 4 )

References 

1

Possemato A (2009) CST Curation Set: 5935; Year: 2009; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

2

Gu T (2005) CST Curation Set: 5404; Year: 2005; Biosample/Treatment: cell line, 293T/bFGF; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

3

Gu T (2005) CST Curation Set: 5390; Year: 2005; Biosample/Treatment: cell line, 293T/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

4

Schlaepfer DD, Jones KC, Hunter T (1998) Multiple Grb2-mediated integrin-stimulated signaling pathways to ERK2/mitogen-activated protein kinase: summation of both c-Src- and focal adhesion kinase-initiated tyrosine phosphorylation events. Mol Cell Biol 18, 2571-85
9566877   Curated Info

5

Sato K, et al. (1997) Tyrosine residues 239 and 240 of Shc are phosphatidylinositol 4,5-bisphosphate-dependent phosphorylation sites by c-Src. Biochem Biophys Res Commun 240, 399-404
9388490   Curated Info

6

Gotoh N, Toyoda M, Shibuya M (1997) Tyrosine phosphorylation sites at amino acids 239 and 240 of Shc are involved in epidermal growth factor-induced mitogenic signaling that is distinct from Ras/mitogen-activated protein kinase activation. Mol Cell Biol 17, 1824-31
9121430   Curated Info

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