Ser11
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Phosphorylation Site Page:
Ser11 - DCK (human)

Site Information
PPKRsCPsFsAssEG   SwissProt Entrez-Gene
Predicted information:  Scansite
Orthologous residues: DCK (mouse): S11, DCK (rat): S11
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 460585

In vivo Characterization
Methods used to characterize site in vivo: [32P] bio-synthetic labeling (15, 16), mass spectrometry (1, 2, 3, 4, 5, 6, 7, 8, 10, 12, 13, 14, 15, 16), mass spectrometry (in vitro) (9), mutation of modification site (11), phosphopeptide mapping (16)
Disease tissue studied: breast cancer (2), breast ductal carcinoma (2), breast cancer, triple negative (2)
Relevant cell line - cell type - tissue: 293T (epithelial) (11, 15, 16), A498 (renal) (10), breast (2), HCT116 (intestinal) (12), HeLa (cervical) (1), HeLa S3 (cervical) (13), Jurkat (T lymphocyte) (3, 4, 5, 6, 7, 8, 14), MV4-11 (macrophage) (12)

Controlled by
Kinases, in vitro: CK1D (human) (9)
Treatments: ischemia (2)

Downstream Regulation
Effects of modification on DCK: phosphorylation (11)


References

1

Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94
25159151   Curated Info

2

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

3

Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7
23749302   Curated Info

4

Mulhern D (2012) CST Curation Set: 13109; Year: 2012; Biosample/Treatment: cell line, Jurkat/calyculin &'||' pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY]
Curated Info

5

Mulhern D (2011) CST Curation Set: 12713; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin &'||' pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]XP Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser) 14-3-3 Binding Motif Antibody Cat#: 9601
Curated Info

6

Guo A (2011) CST Curation Set: 12074; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin &'||' pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: (F/Y/M)Xp[ST](L/I/M)
Curated Info

7

Guo A (2011) CST Curation Set: 11989; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin &'||' pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]XP Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser) 14-3-3 Binding Motif Antibody Cat#: 9601
Curated Info

8

Guo A (2011) CST Curation Set: 11893; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin &'||' pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY]
Curated Info

9

Smal C, et al. (2010) Casein kinase 1delta activates human recombinant deoxycytidine kinase by Ser-74 phosphorylation, but is not involved in the in vivo regulation of its activity. Arch Biochem Biophys 502, 44-52
20637175   Curated Info

10

Schreiber TB, et al. (2010) An integrated phosphoproteomics work flow reveals extensive network regulation in early lysophosphatidic acid signaling. Mol Cell Proteomics 9, 1047-62
20071362   Curated Info

11

Smal C, et al. (2010) Influence of phosphorylation of THR-3, SER-11, and SER-15 on deoxycytidine kinase activity and stability. Nucleosides Nucleotides Nucleic Acids 29, 404-7
20544527   Curated Info

12

Oppermann FS, et al. (2009) Large-scale proteomics analysis of the human kinome. Mol Cell Proteomics 8, 1751-64
19369195   Curated Info

13

Daub H, et al. (2008) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle. Mol Cell 31, 438-48
18691976   Curated Info

14

Stokes M (2008) CST Curation Set: 3884; Year: 2008; Biosample/Treatment: cell line, Jurkat/pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY])
Curated Info

15

Smal C, et al. (2006) Identification of in vivo phosphorylation sites on human deoxycytidine kinase. Role of Ser-74 in the control of enzyme activity. J Biol Chem 281, 4887-93
16361699   Curated Info

16

Smal C, et al. (2006) Identification of phosphorylation sites on human deoxycytidine kinase after overexpression in eucaryotic cells. Nucleosides Nucleotides Nucleic Acids 25, 1141-6
17065079   Curated Info

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