Tyr823
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Phosphorylation Site Page:
Tyr823 - Kit (human)

Site Information
DIKNDSNyVVKGNAR   SwissProt Entrez-Gene
Predicted information:  Scansite
Orthologous residues: Kit (rat): (Y824), Kit (mouse): Y825
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448018
Additional spectra:  2 CST

In vivo Characterization
Methods used to characterize site in vivo: mass spectrometry (1, 2, 4, 6, 7, 8, 9, 10, 11), mass spectrometry (in vitro) (5), mutation of modification site (12)
Disease tissue studied: gastric cancer (2, 6, 8, 9), gastric carcinoma (9), leukemia (10, 11), acute myelogenous leukemia (10, 11), liver cancer (7), neuroblastoma (1, 4)
Relevant cell line - cell type - tissue: CHRF (megakaryocyte) (10), GIST-T1 (gastrointestinal) (2), IC2 (mast) (12), liver (7), M-07e (myeloid) (11), SKNBE(2) (neural crest) (1, 4), stomach (6, 8, 9)

Controlled by
Kinases, in vitro: Kit (human) (5)
Treatments: imatinib (2)



References

1

Palacios-Moreno J, et al. (2015) Neuroblastoma Tyrosine Kinase Signaling Networks Involve FYN and LYN in Endosomes and Lipid Rafts. PLoS Comput Biol 11, e1004130
25884760   Curated Info

2

Takahashi T, et al. (2013) New findings of kinase switching in gastrointestinal stromal tumor under imatinib using phosphoproteomic analysis. Int J Cancer 133, 2737-43
23716303   Curated Info

3

Agarwal S, Kazi JU, Rönnstrand L (2013) Phosphorylation of the Activation Loop Tyrosine 823 in c-Kit Is Crucial for Cell Survival and Proliferation. J Biol Chem 288, 22460-8
23803604   Curated Info

4

Guo A (2010) CST Curation Set: 10551; Year: 2010; Biosample/Treatment: cell line, SKNBE(2)/NGF; Disease: neuroblastoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

5

DiNitto JP, et al. (2010) Function of activation loop tyrosine phosphorylation in the mechanism of c-Kit auto-activation and its implication in sunitinib resistance. J Biochem 147, 601-9
20147452   Curated Info

6

Ren H (2009) CST Curation Set: 8060; Year: 2009; Biosample/Treatment: tissue, gastrointestinal tract/untreated; Disease: gastric cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

7

Gu T (2009) CST Curation Set: 7526; Year: 2009; Biosample/Treatment: tissue, liver/untreated; Disease: liver cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

8

Ren H (2008) CST Curation Set: 4091; Year: 2008; Biosample/Treatment: tissue, gastrointestinal tract/untreated; Disease: gastric cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

9

Ren H (2008) CST Curation Set: 3988; Year: 2008; xenograft, Biosample/Treatment: tissue, gastrointestinal tract/untreated; Disease: gastric carcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

10

Gu T (2005) CST Curation Set: 842; Year: 2005; Biosample/Treatment: cell line, CHRF/-; Disease: acute myelogenous leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

11

Gu T (2005) CST Curation Set: 723; Year: 2005; Biosample/Treatment: cell line, M-07e/-; Disease: acute myelogenous leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

12

Koma Y, et al. (2005) Distinct role for c-kit receptor tyrosine kinase and SgIGSF adhesion molecule in attachment of mast cells to fibroblasts. Lab Invest 85, 426-35
15654360   Curated Info

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