Lys401
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Ubiquitylation Site Page:
Lys401 - PML (human)

Site Information
KDAAVSKkASPEAAS   SwissProt Entrez-Gene
Predicted information:  Scansite
Orthologous residues: PML iso8 (human): (K401), PML (rat): (‑), PML iso2 (mouse): (‑), PML iso14 (human): (K401), PML iso10 (human): (K401), PML iso11 (human): (K401), PML iso2 (human): (K401), PML iso4 (human): (K401), PML (mouse): (‑), PML iso3 (human): (K401)
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 2885407

In vivo Characterization
Methods used to characterize site in vivo: mass spectrometry (1, 2, 3, 4, 5, 6)
Disease tissue studied: multiple myeloma (3, 5)
Relevant cell line - cell type - tissue: Jurkat (T lymphocyte) (1), KMS-12-BM (B lymphocyte) (5), L-363 (plasma cell) (3), U266 (plasma cell) (6)




References

1

Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7
23749302   Curated Info

2

Kim W, et al. (2011) Systematic and quantitative assessment of the ubiquitin-modified proteome. Mol Cell 44, 325-40
21906983   Curated Info

3

(2010) CST Curation Set: 10366; Year: 2010; Biosample/Treatment: cell line, L363/untreated; Disease: -; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-K-e-GG Antibody Used to Purify Peptides prior to MS2: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990
Curated Info

4

(2009) CST Curation Set: 8657; Year: 2009; Biosample/Treatment: cell line, KMS-12-BM/Velcade; Disease: -; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-K-e-GG Antibody Used to Purify Peptides prior to MS2: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990
Curated Info

5

(2009) CST Curation Set: 8658; Year: 2009; Biosample/Treatment: cell line, KMS-12-BM/Velcade; Disease: -; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-K-e-GG Antibody Used to Purify Peptides prior to MS2: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990
Curated Info

6

(2009) CST Curation Set: 8645; Year: 2009; Biosample/Treatment: cell line, U266/Velcade; Disease: -; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-K-e-GG Antibody Used to Purify Peptides prior to MS2: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990
Curated Info

7

Lallemand-Breitenbach V, et al. (2008) Arsenic degrades PML or PML-RARalpha through a SUMO-triggered RNF4/ubiquitin-mediated pathway. Nat Cell Biol 10, 547-55
18408733   Curated Info

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