Tyr1361
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Phosphorylation Site Page:
Tyr1361 - INSR (human)

Site Information
SYEEHIPyTHMNGGK   SwissProt Entrez-Gene
Predicted information:  Scansite
Orthologous residues: INSR iso2 (human): Y1349, INSR (rat): Y1362, INSR (mouse): Y1351
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447684

In vivo Characterization
Methods used to characterize site in vivo: 2D analysis (19), immunoprecipitation (21), mass spectrometry (1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14), mutation of modification site (17, 19, 20, 21), phospho-antibody (17, 21, 22), phosphoamino acid analysis (19), phosphopeptide mapping (19), western blotting (17)
Disease tissue studied: gastric cancer (3, 4, 8, 12, 13), gastric carcinoma (3, 4, 8, 13), neuroblastoma (1, 7, 14), ovarian cancer (6)
Relevant cell line - cell type - tissue: BEAS-2B (epithelial) [LKB1 (human), no information] (10, 11), CHO (fibroblast) (20), CHO (fibroblast) [EphB1 (human), transfection] (21), COS (fibroblast) (19), HCT116 (intestinal) (2), hepatocyte [INSR (mouse)] (17), HES-3 ('stem, embryonic') (5), IM95 (gastric) (12), KMS-11 (B lymphocyte) (9), LAN-1 (neural crest) (1, 14), MEF (fibroblast) [INSR (human), transfection] (22), MKN-45 (gastric) (3, 4, 8, 13), OVKATE (ovarian) (6), SKNBE(2) (neural crest) (1, 7)

Controlled by
Kinases, in vitro: INSR (human) (15, 16, 18, 21, 23, 24)
Phosphatases, in vitro: PTPRA (rat) (23)
Treatments: insulin (17, 19)

Downstream Regulation
Effects of modification on INSR: enzymatic activity, induced (15, 16), protein conformation (15)
Effects of modification on biological processes: cell cycle regulation (20), cell growth, altered (22)


References

1

Palacios-Moreno J, et al. (2015) Neuroblastoma Tyrosine Kinase Signaling Networks Involve FYN and LYN in Endosomes and Lipid Rafts. PLoS Comput Biol 11, e1004130
25884760   Curated Info

2

Stokes MP, et al. (2012) PTMScan Direct: Identification and Quantification of Peptides from Critical Signaling Proteins by Immunoaffinity Enrichment Coupled with LC-MS/MS. Mol Cell Proteomics 11, 187-201
22322096   Curated Info

3

(2012) CST Curation Set: 13326; Year: 2012; Biosample/Treatment: cell line, MKN-45/untreated; Disease: gastric carcinoma; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

4

(2011) CST Curation Set: 12519; Year: 2011; Biosample/Treatment: cell line, MKN-45/normal; Disease: gastric carcinoma; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

5

Ding VM, et al. (2011) Tyrosine phosphorylation profiling in fgf-2 stimulated human embryonic stem cells. PLoS One 6, e17538
21437283   Curated Info

6

(2010) CST Curation Set: 10743; Year: 2010; Biosample/Treatment: cell line, OVKATE/serum starved; Disease: ovarian cancer; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

7

(2010) CST Curation Set: 10551; Year: 2010; Biosample/Treatment: cell line, SKNBE(2)/NGF; Disease: neuroblastoma; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

8

(2010) CST Curation Set: 9924; Year: 2010; Biosample/Treatment: cell line, MKN-45/DMSO; Disease: gastric carcinoma; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-[sty](phosphorylation)
Curated Info

9

St-Germain JR, et al. (2009) Multiple myeloma phosphotyrosine proteomic profile associated with FGFR3 expression, ligand activation, and drug inhibition. Proc Natl Acad Sci U S A 106, 20127-32
19901323   Curated Info

10

(2009) CST Curation Set: 6681; Year: 2009; Biosample/Treatment: cell line, Dana Farber BEAS LKB/untreated; Disease: -; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

11

(2009) CST Curation Set: 6682; Year: 2009; Biosample/Treatment: cell line, Dana Farber BEAS LKB/untreated; Disease: -; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

12

(2008) CST Curation Set: 3917; Year: 2008; Biosample/Treatment: cell line, IM95/serum starved; Disease: gastric cancer; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

13

Guo A, et al. (2008) Signaling networks assembled by oncogenic EGFR and c-Met. Proc Natl Acad Sci U S A 105, 692-7
18180459   Curated Info

14

(2006) CST Curation Set: 2043; Year: 2006; Biosample/Treatment: cell line, LAN-1/serum starved; Disease: -; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

15

Tennagels N, Bergschneider E, Al-Hasani H, Klein HW (2000) Autophosphorylation of the two C-terminal tyrosine residues Tyr1316 and Tyr1322 modulates the activity of the insulin receptor kinase in vitro. FEBS Lett 479, 67-71
10940390   Curated Info

16

Noelle V, Tennagels N, Klein HW (2000) A single substitution of the insulin receptor kinase inhibits serine autophosphorylation in vitro: evidence for an interaction between the C-terminus and the activation loop. Biochemistry 39, 7170-7
10852715   Curated Info

17

Soni P, et al. (2000) The differential effects of pp120 (Ceacam 1) on the mitogenic action of insulin and insulin-like growth factor 1 are regulated by the nonconserved tyrosine 1316 in the insulin receptor. Mol Cell Biol 20, 3896-905
10805733   Curated Info

18

Al-Hasani H, et al. (1997) Identification of Ser-1275 and Ser-1309 as autophosphorylation sites of the insulin receptor. FEBS Lett 400, 65-70
9000514   Curated Info

19

Lewis RE, Volle DJ, Sanderson SD (1994) Phorbol ester stimulates phosphorylation on serine 1327 of the human insulin receptor. J Biol Chem 269, 26259-66
7929343   Curated Info

20

Ando A, et al. (1992) Enhanced insulin-induced mitogenesis and mitogen-activated protein kinase activities in mutant insulin receptors with substitution of two COOH-terminal tyrosine autophosphorylation sites by phenylalanine. J Biol Chem 267, 12788-96
1618780   Curated Info

21

Murakami MS, Rosen OM (1991) The role of insulin receptor autophosphorylation in signal transduction. J Biol Chem 266, 22653-60
1658004   Curated Info

22

Baron V, et al. (1991) The carboxyl-terminal domain of the insulin receptor: its potential role in growth-promoting effects. Biochemistry 30, 9365-70
1654097   Curated Info

23

King MJ, Sale GJ (1990) Dephosphorylation of insulin-receptor autophosphorylation sites by particulate and soluble phosphotyrosyl-protein phosphatases. Biochem J 266, 251-9
1689998   Curated Info

24

Tavaré JM, Denton RM (1988) Studies on the autophosphorylation of the insulin receptor from human placenta. Analysis of the sites phosphorylated by two-dimensional peptide mapping. Biochem J 252, 607-15
3166375   Curated Info

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