Phosphorylation Site Page:
In vivo Characterization
Methods used to characterize site
mass spectrometry ( 1, 2, 3, 4)
Relevant cell line - cell type - tissue:
brain ( 3, 4), MEF (fibroblast) [p53 (mouse), homozygous knockout] ( 2), PC-12 (chromaffin) ( 1), PC-12 (chromaffin) [TrkA (rat), transfection] ( 1)
Biarc J, Chalkley RJ, Burlingame AL, Bradshaw RA (2012) The induction of serine/threonine protein phosphorylations by a PDGFR/TrkA chimera in stably transfected PC12 cells.
Mol Cell Proteomics 11, 15-30
Hsu PP, et al. (2011) The mTOR-regulated phosphoproteome reveals a mechanism of mTORC1-mediated inhibition of growth factor signaling.
Science 332, 1317-22
Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression.
Cell 143, 1174-89
Wiśniewski JR, et al. (2010) Brain phosphoproteome obtained by a FASP-based method reveals plasma membrane protein topology.
J Proteome Res 9, 3280-9