Lys328
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Ubiquitylation Site Page:
Lys328 - ACTA2 (mouse)

Site Information
ALAPstMkIkIIAPP   SwissProt Entrez-Gene
Predicted information:  Scansite
Orthologous residues: ACTA2 (human): K328, ACTA2 (rat): (K328)
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 3945336

In vivo Characterization
Methods used to characterize site in vivo: mass spectrometry (1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 29, 30, 31, 32)
Disease tissue studied: leukemia (18), testicular cancer (4, 5)
Relevant cell line - cell type - tissue: 'brain, embryonic' (31), 'stem, embryonic' (6, 7, 8, 9, 10, 11, 12, 13), brain (1, 32), ES-J1 (stem) (3), F9 (testicular) (4), heart (1, 29), kidney (1), liver (1, 30), muscle (1, 17), P19 (testicular) (5), pooled tissues (1), PYS-2 (epithelial) (2), RAW 264.7 (macrophage) (18), spleen (14), testis (15), thymus (16)




References

1

Wagner SA, et al. (2012) Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues. Mol Cell Proteomics 11, 1578-85
22790023   Curated Info

2

Zhou J (2010) CST Curation Set: 9134; Year: 2010; Biosample/Treatment: cell line, PY2/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG)
Curated Info

3

Zhou J (2010) CST Curation Set: 9129; Year: 2010; Biosample/Treatment: cell line, ES J1/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG)
Curated Info

4

Zhou J (2010) CST Curation Set: 9130; Year: 2010; Biosample/Treatment: cell line, F9/untreated; Disease: testicular cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG)
Curated Info

5

Zhou J (2010) CST Curation Set: 9133; Year: 2010; Biosample/Treatment: cell line, P19/untreated; Disease: testicular cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG)
Curated Info

6

Tucker M (2010) CST Curation Set: 9089; Year: 2010; Biosample/Treatment: cell line, embryonic stem cells (mouse)/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG)
Curated Info

7

Tucker M (2010) CST Curation Set: 9088; Year: 2010; Biosample/Treatment: cell line, embryonic stem cells (mouse)/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG)
Curated Info

8

Tucker M (2010) CST Curation Set: 9085; Year: 2010; Biosample/Treatment: cell line, embryonic stem cells (mouse)/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG)
Curated Info

9

Tucker M (2010) CST Curation Set: 9087; Year: 2010; Biosample/Treatment: cell line, embryonic stem cells (mouse)/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG)
Curated Info

10

Tucker M (2010) CST Curation Set: 9086; Year: 2010; Biosample/Treatment: cell line, embryonic stem cells (mouse)/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG)
Curated Info

11

Tucker M (2010) CST Curation Set: 9084; Year: 2010; Biosample/Treatment: cell line, embryonic stem cells (mouse)/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG)
Curated Info

12

Tucker M (2010) CST Curation Set: 9083; Year: 2010; Biosample/Treatment: cell line, embryonic stem cells (mouse)/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG)
Curated Info

13

Tucker M (2010) CST Curation Set: 9090; Year: 2010; Biosample/Treatment: cell line, embryonic stem cells (mouse)/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG)
Curated Info

14

Guo A (2009) CST Curation Set: 8159; Year: 2009; Biosample/Treatment: tissue, spleen/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG) Antibodies Used to Purify Peptides prior to LCMS: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990
Curated Info

15

Guo A (2009) CST Curation Set: 8160; Year: 2009; Biosample/Treatment: tissue, testis/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG) Antibodies Used to Purify Peptides prior to LCMS: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990
Curated Info

16

Guo A (2009) CST Curation Set: 8161; Year: 2009; Biosample/Treatment: tissue, thymus/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG) Antibodies Used to Purify Peptides prior to LCMS: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990
Curated Info

17

Guo A (2009) CST Curation Set: 8158; Year: 2009; Biosample/Treatment: tissue, muscle/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG) Antibodies Used to Purify Peptides prior to LCMS: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990
Curated Info

18

Possemato A (2009) CST Curation Set: 6916; Year: 2009; Biosample/Treatment: cell line, RAW 264.7/untreated; Disease: leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG)
Curated Info

19

Guo A (2008) CST Curation Set: 4120; Year: 2008; Biosample/Treatment: tissue, brain/untreated; Disease: Alzheimer's disease; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG)
Curated Info

20

Guo A (2008) CST Curation Set: 4121; Year: 2008; Biosample/Treatment: tissue, brain/untreated; Disease: Alzheimer's disease; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG)
Curated Info

21

Guo A (2008) CST Curation Set: 4122; Year: 2008; Biosample/Treatment: tissue, brain/untreated; Disease: Alzheimer's disease; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG)
Curated Info

22

Guo A (2008) CST Curation Set: 4123; Year: 2008; Biosample/Treatment: tissue, brain/untreated; Disease: Alzheimer's disease; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG)
Curated Info

23

Guo A (2008) CST Curation Set: 4124; Year: 2008; Biosample/Treatment: tissue, brain/untreated; Disease: Alzheimer's disease; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG)
Curated Info

24

Guo A (2008) CST Curation Set: 4125; Year: 2008; Biosample/Treatment: tissue, brain/untreated; Disease: Alzheimer's disease; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG)
Curated Info

25

Guo A (2008) CST Curation Set: 4126; Year: 2008; Biosample/Treatment: tissue, brain/untreated; Disease: Alzheimer's disease; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG)
Curated Info

26

Guo A (2008) CST Curation Set: 4127; Year: 2008; Biosample/Treatment: tissue, brain/untreated; Disease: Alzheimer's disease; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG)
Curated Info

27

Guo A (2008) CST Curation Set: 4128; Year: 2008; Biosample/Treatment: tissue, brain/untreated; Disease: Alzheimer's disease; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG)
Curated Info

28

Guo A (2008) CST Curation Set: 4129; Year: 2008; Biosample/Treatment: tissue, brain/untreated; Disease: Alzheimer's disease; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG)
Curated Info

29

Guo A (2007) CST Curation Set: 3114; Year: 2007; Biosample/Treatment: tissue, heart/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG)
Curated Info

30

Guo A (2007) CST Curation Set: 3115; Year: 2007; Biosample/Treatment: tissue, liver/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG)
Curated Info

31

Guo A (2007) CST Curation Set: 3116; Year: 2007; Biosample/Treatment: tissue, brain, embryonic/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG)
Curated Info

32

Guo A (2007) CST Curation Set: 3117; Year: 2007; Biosample/Treatment: tissue, brain/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG)
Curated Info

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