Ser57
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Phosphorylation Site Page:
Ser57 - PWP1 (human)

Site Information
sDEEEtGsPsEDGMQ   SwissProt Entrez-Gene
Predicted information:  Scansite
Orthologous residues: PWP1 (rat): S56, PWP1 (mouse): (N56)
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 452815

In vivo Characterization
Methods used to characterize site in vivo: mass spectrometry (1, 2, 3, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
Disease tissue studied: breast cancer (1, 3, 7), cervical cancer (11), cervical adenocarcinoma (11), leukemia (17), chronic myelogenous leukemia (17), lung cancer (7), non-small cell lung cancer (7), melanoma skin cancer (2)
Relevant cell line - cell type - tissue: 293 (epithelial) (13), 293 (epithelial) [AT1 (human), transfection, AT1R stable transfected HEK293] (10), 293 (epithelial) [AT1 (human), transfection] (9), BT-20 (breast cell) (7), BT-474 (breast cell) (1), Calu 6 (pulmonary) (7), Flp-In T-Rex-293 (epithelial) (8), Flp-In T-Rex-293 (epithelial) [PRKD1 (human), genetic knockin] (8), H1395 (pulmonary) (7), H2009 (pulmonary) (7), H2077 (pulmonary) (7), H2887 (pulmonary) (7), H322 (pulmonary) (7), H322M (pulmonary) (7), HCC2279 (pulmonary) (7), HCC366 (pulmonary) (7), HCC78 (pulmonary) (7), HeLa (cervical) (6, 15, 18), HeLa S3 (cervical) (11, 12), HMLER ('stem, breast cancer') (3), HMLER ('stem, breast cancer') [CXCR4 (human), knockdown] (3), HOP62 (pulmonary) (7), Jurkat (T lymphocyte) (5, 14), K562 (erythroid) (6, 16, 17), LOU-NH91 (squamous) (7), MCF-7 (breast cell) (1), MDA-MB468 (breast cell) (7), NCI-H1568 (pulmonary) (7), NCI-H1648 (pulmonary) (7), NCI-H2030 (pulmonary) (7), NCI-H2172 (pulmonary) (7), NCI-H460 (pulmonary) (7), NCI-H647 (pulmonary) (7), WM239A (epidermal) (2)

Controlled by
Treatments: nocodazole (11)



References

1

Carrier M, et al. (2016) Phosphoproteome and Transcriptome of RA-Responsive and RA-Resistant Breast Cancer Cell Lines. PLoS One 11, e0157290
27362937   Curated Info

2

Stuart SA, et al. (2015) A Phosphoproteomic Comparison of B-RAFV600E and MKK1/2 Inhibitors in Melanoma Cells. Mol Cell Proteomics 14, 1599-615
25850435   Curated Info

3

Yi T, et al. (2014) Quantitative phosphoproteomic analysis reveals system-wide signaling pathways downstream of SDF-1/CXCR4 in breast cancer stem cells. Proc Natl Acad Sci U S A 111, E2182-90
24782546   Curated Info

4

Schweppe DK, Rigas JR, Gerber SA (2013) Quantitative phosphoproteomic profiling of human non-small cell lung cancer tumors. J Proteomics 91, 286-96
23911959   Curated Info

5

Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7
23749302   Curated Info

6

Zhou H, et al. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res 12, 260-71
23186163   Curated Info

7

Klammer M, et al. (2012) Phosphosignature predicts dasatinib response in non-small cell lung cancer. Mol Cell Proteomics 11, 651-68
22617229   Curated Info

8

Franz-Wachtel M, et al. (2012) Global detection of protein kinase D-dependent phosphorylation events in nocodazole-treated human cells. Mol Cell Proteomics 11, 160-70
22496350   Curated Info

9

Xiao K, et al. (2010) Global phosphorylation analysis of beta-arrestin-mediated signaling downstream of a seven transmembrane receptor (7TMR). Proc Natl Acad Sci U S A 107, 15299-304
20686112   Curated Info

10

Christensen GL, et al. (2010) Quantitative phosphoproteomics dissection of seven-transmembrane receptor signaling using full and biased agonists. Mol Cell Proteomics 9, 1540-53
20363803   Curated Info

11

Olsen JV, et al. (2010) Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal 3, ra3
20068231   Curated Info

12

Malik R, et al. (2009) Quantitative analysis of the human spindle phosphoproteome at distinct mitotic stages. J Proteome Res 8, 4553-63
19691289   Curated Info

13

Gauci S, et al. (2009) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach. Anal Chem 81, 4493-501
19413330   Curated Info

14

Mayya V, et al. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions. Sci Signal 2, ra46
19690332   Curated Info

15

Dephoure N, et al. (2008) A quantitative atlas of mitotic phosphorylation. Proc Natl Acad Sci U S A 105, 10762-7
18669648   Curated Info

16

Stokes M (2008) CST Curation Set: 4609; Year: 2008; Biosample/Treatment: cell line, K562/untreated; Disease: chronic myelogenous leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY])
Curated Info

17

Stokes M (2008) CST Curation Set: 4393; Year: 2008; Biosample/Treatment: cell line, K562/untreated; Disease: chronic myelogenous leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY])
Curated Info

18

Beausoleil SA, et al. (2004) Large-scale characterization of HeLa cell nuclear phosphoproteins. Proc Natl Acad Sci U S A 101, 12130-5
15302935   Curated Info

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