Phosphorylation Site Page:
In vivo Characterization
Methods used to characterize site
mass spectrometry ( 1, 3, 4, 5, 6, 7, 8)
Disease tissue studied:
leukemia ( 5), acute myelogenous leukemia ( 5), neuroblastoma ( 6)
Relevant cell line - cell type - tissue:
'3T3-L1, differentiated' (adipocyte) ( 1, 3), blood ( 5), brain ( 8), macrophage-bone marrow ( 7), macrophage-bone marrow [DUSP1 (mouse), homozygous knockout] ( 7), macrophage-peritoneum [RIP3 (mouse), homozygous knockout] ( 4), N1E-115 (neuron) ( 6)
Parker BL, et al. (2015) Targeted phosphoproteomics of insulin signaling using data-independent acquisition mass spectrometry.
Sci Signal 8, rs6
Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels.
Mol Cell Proteomics 13, 1690-704
Humphrey SJ, et al. (2013) Dynamic Adipocyte Phosphoproteome Reveals that Akt Directly Regulates mTORC2.
Cell Metab 17, 1009-20
Wu X, et al. (2012) Investigation of receptor interacting protein (RIP3)-dependent protein phosphorylation by quantitative phosphoproteomics.
Mol Cell Proteomics 11, 1640-51
Trost M, et al. (2012) Posttranslational regulation of self-renewal capacity: insights from proteome and phosphoproteome analyses of stem cell leukemia.
Blood 120, e17-27
Wang Y, et al. (2011) Spatial phosphoprotein profiling reveals a compartmentalized extracellular signal-regulated kinase switch governing neurite growth and retraction.
J Biol Chem 286, 18190-201
Weintz G, et al. (2010) The phosphoproteome of toll-like receptor-activated macrophages.
Mol Syst Biol 6, 371
Wiśniewski JR, et al. (2010) Brain phosphoproteome obtained by a FASP-based method reveals plasma membrane protein topology.
J Proteome Res 9, 3280-9