Lys29
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Ubiquitylation Site Page:
Lys29 - galectin-1 (human)

Site Information
GEVAPDAksFVLNLG   SwissProt Entrez-Gene
Predicted information:  Scansite
Orthologous residues: galectin‑1 (mouse): K29, galectin‑1 (rat): (K29)
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 15389025

In vivo Characterization
Methods used to characterize site in vivo: mass spectrometry (1, 2, 3, 4, 5, 6)
Disease tissue studied: multiple myeloma (3, 4, 5)
Relevant cell line - cell type - tissue: 293T (epithelial) (2), AMO-1 (plasma cell) (3, 4, 5), HeLa (cervical) (6), MV4-11 (macrophage) (2)

Controlled by
Treatments: MG132 (2)



References

1

Kim W, et al. (2011) Systematic and quantitative assessment of the ubiquitin-modified proteome. Mol Cell 44, 325-40
21906983   Curated Info

2

Wagner SA, et al. (2011) A Proteome-wide, Quantitative Survey of In Vivo Ubiquitylation Sites Reveals Widespread Regulatory Roles. Mol Cell Proteomics 10, M111.013284
21890473   Curated Info

3

Guo A (2010) CST Curation Set: 9913; Year: 2010; Biosample/Treatment: cell line, AMO-1/Velcade; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG) Antibodies Used to Purify Peptides prior to LCMS: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990
Curated Info

4

Guo A (2010) CST Curation Set: 9914; Year: 2010; Biosample/Treatment: cell line, AMO-1/Velcade; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG) Antibodies Used to Purify Peptides prior to LCMS: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990
Curated Info

5

Guo A (2010) CST Curation Set: 9915; Year: 2010; Biosample/Treatment: cell line, AMO-1/Velcade; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG) Antibodies Used to Purify Peptides prior to LCMS: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990
Curated Info

6

Moritz A (2010) CST Curation Set: 9062; Year: 2010; Biosample/Treatment: cell line, HeLa/untreated; Disease: cervical adenocarcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG)
Curated Info

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