Ser431
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Phosphorylation Site Page:
Ser431 - abLIM (human)

Site Information
GEsPRtLsPtPsAEG   SwissProt Entrez-Gene
Predicted information:  Scansite
Orthologous residues: abLIM iso5 (human): (S143), abLIM (mouse): S475, abLIM (rat): S115, abLIM iso2 (mouse): (S355), abLIM iso3 (mouse): (S355), abLIM iso2 (human): (S371)
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 451847

In vivo Characterization
Methods used to characterize site in vivo: mass spectrometry (1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34)
Disease tissue studied: bone cancer (21), osteosarcoma (21), breast cancer (3, 8, 9), breast ductal carcinoma (3), breast cancer, triple negative (3), cervical cancer (22), cervical adenocarcinoma (22), leukemia (11), acute myelogenous leukemia (11), hepatocellular carcinoma, surrounding tissue (20), lung cancer (4, 9), non-small cell lung cancer (9), non-small cell lung adenocarcinoma (4), ovarian cancer (3)
Relevant cell line - cell type - tissue: 'muscle, skeletal' (12, 23), 'stem, embryonic' (25), 293 (epithelial) (26), 293 (epithelial) [AT1 (human), transfection, AT1R stable transfected HEK293] (19), 293T (epithelial) (1), A549 (pulmonary) (5), breast (3), BT-20 (breast cell) (9), BT-549 (breast cell) (9), Calu 6 (pulmonary) (9), DG75 (B lymphocyte) (18), Flp-In T-Rex-293 (epithelial) (10), Flp-In T-Rex-293 (epithelial) [PRKD1 (human), genetic knockin] (10), H1395 (pulmonary) (9), H2009 (pulmonary) (9), H2077 (pulmonary) (9), H2887 (pulmonary) (9), H322 (pulmonary) (9), H322M (pulmonary) (9), HCC1359 (pulmonary) (9), HCC1937 (breast cell) (9), HCC2279 (pulmonary) (9), HCC366 (pulmonary) (9), HCC4006 (pulmonary) (9), HCC78 (pulmonary) (9), HCC827 (pulmonary) (9), HeLa (cervical) (2, 7, 13, 14, 17, 28, 29, 33), HeLa S3 (cervical) (22), hepatocyte-liver (20), HOP62 (pulmonary) (9), HUES-7 ('stem, embryonic') (24), Jurkat (T lymphocyte) (6, 15, 27, 31, 32, 34), KG-1 (myeloid) (11), LCLC-103H (pulmonary) (9), leukocyte-blood (21), LOU-NH91 (squamous) (9), lung (4), MCF-7 (breast cell) (9), MDA-MB231 (breast cell) (9), MDA-MB468 (breast cell) (9), NCI-H1568 (pulmonary) (9), NCI-H157 (pulmonary) (9), NCI-H1648 (pulmonary) (9), NCI-H1666 (pulmonary) (9), NCI-H2030 (pulmonary) (9), NCI-H2172 (pulmonary) (9), NCI-H460 (pulmonary) (9), NCI-H520 (squamous) (9), NCI-H647 (pulmonary) (9), ovary (3), PC9 (pulmonary) (9), platelet-blood (30), SKBr3 (breast cell) (8), U2OS (bone cell) (21)

Controlled by
Treatments: anti-CD3 (27), BI2536 (17), ischemia (3), nocodazole (22)



References

1

Franchin C, et al. (2015) Quantitative analysis of a phosphoproteome readily altered by the protein kinase CK2 inhibitor quinalizarin in HEK-293T cells. Biochim Biophys Acta 1854, 609-23
25278378   Curated Info

2

Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94
25159151   Curated Info

3

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

4

Schweppe DK, Rigas JR, Gerber SA (2013) Quantitative phosphoproteomic profiling of human non-small cell lung cancer tumors. J Proteomics 91, 286-96
23911959   Curated Info

5

Kim JY, et al. (2013) Dissection of TBK1 signaling via phosphoproteomics in lung cancer cells. Proc Natl Acad Sci U S A 110, 12414-9
23836654   Curated Info

6

Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7
23749302   Curated Info

7

Zhou H, et al. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res 12, 260-71
23186163   Curated Info

8

Imami K, et al. (2012) Temporal profiling of lapatinib-suppressed phosphorylation signals in EGFR/HER2 pathways. Mol Cell Proteomics 11, 1741-57
22964224   Curated Info

9

Klammer M, et al. (2012) Phosphosignature predicts dasatinib response in non-small cell lung cancer. Mol Cell Proteomics 11, 651-68
22617229   Curated Info

10

Franz-Wachtel M, et al. (2012) Global detection of protein kinase D-dependent phosphorylation events in nocodazole-treated human cells. Mol Cell Proteomics 11, 160-70
22496350   Curated Info

11

Weber C, Schreiber TB, Daub H (2012) Dual phosphoproteomics and chemical proteomics analysis of erlotinib and gefitinib interference in acute myeloid leukemia cells. J Proteomics 75, 1343-56
22115753   Curated Info

12

Lundby A, et al. (2012) Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun 3, 876
22673903   Curated Info

13

Nishioka T, Nakayama M, Amano M, Kaibuchi K (2012) Proteomic screening for Rho-kinase substrates by combining kinase and phosphatase inhibitors with 14-3-3ζ affinity chromatography. Cell Struct Funct 37, 39-48
22251793   Curated Info

14

Grosstessner-Hain K, et al. (2011) Quantitative phospho-proteomics to investigate the polo-like kinase 1-dependent phospho-proteome. Mol Cell Proteomics 10, M111.008540
21857030   Curated Info

15

Guo A (2011) CST Curation Set: 11892; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin &'||' pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY]
Curated Info

16

Rigbolt KT, et al. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation. Sci Signal 4, rs3
21406692   Curated Info

17

Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5
21712546   Curated Info

18

Iliuk AB, et al. (2010) In-depth analyses of kinase-dependent tyrosine phosphoproteomes based on metal ion-functionalized soluble nanopolymers. Mol Cell Proteomics 9, 2162-72
20562096   Curated Info

19

Christensen GL, et al. (2010) Quantitative phosphoproteomics dissection of seven-transmembrane receptor signaling using full and biased agonists. Mol Cell Proteomics 9, 1540-53
20363803   Curated Info

20

Han G, et al. (2010) Phosphoproteome analysis of human liver tissue by long-gradient nanoflow LC coupled with multiple stage MS analysis. Electrophoresis 31, 1080-9
20166139   Curated Info

21

Raijmakers R, et al. (2010) Exploring the human leukocyte phosphoproteome using a microfluidic reversed-phase-TiO2-reversed-phase high-performance liquid chromatography phosphochip coupled to a quadrupole time-of-flight mass spectrometer. Anal Chem 82, 824-32
20058876   Curated Info

22

Olsen JV, et al. (2010) Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal 3, ra3
20068231   Curated Info

23

Højlund K, et al. (2009) In vivo phosphoproteome of human skeletal muscle revealed by phosphopeptide enrichment and HPLC-ESI-MS/MS. J Proteome Res 8, 4954-65
19764811   Curated Info

24

Van Hoof D, et al. (2009) Phosphorylation dynamics during early differentiation of human embryonic stem cells. Cell Stem Cell 5, 214-26
19664995   Curated Info

25

Brill LM, et al. (2009) Phosphoproteomic analysis of human embryonic stem cells. Cell Stem Cell 5, 204-13
19664994   Curated Info

26

Gauci S, et al. (2009) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach. Anal Chem 81, 4493-501
19413330   Curated Info

27

Mayya V, et al. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions. Sci Signal 2, ra46
19690332   Curated Info

28

Dephoure N, et al. (2008) A quantitative atlas of mitotic phosphorylation. Proc Natl Acad Sci U S A 105, 10762-7
18669648   Curated Info

29

Ruse CI, et al. (2008) Motif-specific sampling of phosphoproteomes. J Proteome Res 7, 2140-50
18452278   Curated Info

30

Zahedi RP, et al. (2008) Phosphoproteome of resting human platelets. J Proteome Res 7, 526-34
18088087   Curated Info

31

Stokes M (2007) CST Curation Set: 2935; Year: 2007; Biosample/Treatment: cell line, Jurkat/pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

32

Ficarro SB, et al. (2005) Automated immobilized metal affinity chromatography/nano-liquid chromatography/electrospray ionization mass spectrometry platform for profiling protein phosphorylation sites. Rapid Commun Mass Spectrom 19, 57-71
15570572   Curated Info

33

Beausoleil SA, et al. (2004) Large-scale characterization of HeLa cell nuclear phosphoproteins. Proc Natl Acad Sci U S A 101, 12130-5
15302935   Curated Info

34

Brill LM, et al. (2004) Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry. Anal Chem 76, 2763-72
15144186   Curated Info

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