Lys25
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Sumoylation Site Page:
Lys25 - SUMO1 (human)

Site Information
EGEYIkLkVIGQDSs   SwissProt Entrez-Gene
Predicted information:  Scansite
Orthologous residues: SUMO1 (rat): (K25), SUMO1 (mouse): (K25), SUMO1 iso2 (human): (‑)
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 13875704

In vivo Characterization
Methods used to characterize site in vivo: mass spectrometry (1, 2), western blotting (2)
Relevant cell line - cell type - tissue: HeLa (cervical) (2), HeLa (cervical) [SUMO2 (human), transfection, SUMO2 K0 Q87R (lysine-deficient SUMO2, with Q87R to create convenient Trypsin cleavage site)] (1)




References

1

Hendriks IA, et al. (2014) Uncovering global SUMOylation signaling networks in a site-specific manner. Nat Struct Mol Biol 21, 927-36
25218447   Curated Info

2

Blomster HA, et al. (2010) In vivo identification of sumoylation sites by a signature tag and cysteine-targeted affinity purification. J Biol Chem 285, 19324-9
20388717   Curated Info

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