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Phosphorylation Site Page:
Ser283 - IkB-alpha (human)

Site Information
NLQMLPEsEDEESYD   SwissProt Entrez-Gene
Predicted information:  Scansite
Orthologous residues: IkB‑alpha (mouse): (S283), IkB‑alpha (rat): (S283)
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 451523

In vivo Characterization
Methods used to characterize site in vivo: [32P] bio-synthetic labeling (4), mass spectrometry (1), mutation of modification site (3), phosphopeptide mapping (4), western blotting (3)
Relevant cell line - cell type - tissue: 3T3 (fibroblast) (3), Jurkat (T lymphocyte) (4), liver (1)

Controlled by
Putative upstream kinases: CK2A1 (human) (4)
Kinases, in vitro: CK2A1 (human) (2, 3, 4)

Downstream Regulation
Effects of modification on IkB-alpha: protein degradation (3)



Bian Y, et al. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J Proteomics 96, 253-62
24275569   Curated Info


Shen J, Channavajhala P, Seldin DC, Sonenshein GE (2001) Phosphorylation by the protein kinase CK2 promotes calpain-mediated degradation of IkappaBalpha. J Immunol 167, 4919-25
11673497   Curated Info


Lin R, et al. (1996) Phosphorylation of IkappaBalpha in the C-terminal PEST domain by casein kinase II affects intrinsic protein stability. Mol Cell Biol 16, 1401-9
8657113   Curated Info


McElhinny JA, et al. (1996) Casein kinase II phosphorylates I kappa B alpha at S-283, S-289, S-293, and T-291 and is required for its degradation. Mol Cell Biol 16, 899-906
8622692   Curated Info

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