Lys50
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Ubiquitylation Site Page:
Lys50 - GSTM1 (human)

Site Information
RSQWLNEkFKLGLDF   SwissProt Entrez-Gene
Predicted information:  Scansite
Orthologous residues: GSTM1 (rat): (K50), GSTM1 (mouse): K50, GSTM1 iso2 (human): (K50)
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 10839285

In vivo Characterization
Methods used to characterize site in vivo: mass spectrometry (1, 2, 3, 4, 5, 6)
Disease tissue studied: multiple myeloma (2, 3, 6)
Relevant cell line - cell type - tissue: AMO-1 (plasma cell) (3), U266 (plasma cell) (2, 4, 5, 6)




References

1

Kim W, et al. (2011) Systematic and quantitative assessment of the ubiquitin-modified proteome. Mol Cell 44, 325-40
21906983   Curated Info

2

Guo A (2010) CST Curation Set: 10264; Year: 2010; Biosample/Treatment: cell line, U266/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG) Antibodies Used to Purify Peptides prior to LCMS: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990
Curated Info

3

Guo A (2010) CST Curation Set: 8771; Year: 2009; Biosample/Treatment: cell line, AMO-1/Velcade; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG) Antibodies Used to Purify Peptides prior to LCMS: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990
Curated Info

4

Guo A (2009) CST Curation Set: 8646; Year: 2009; Biosample/Treatment: cell line, U266/Velcade; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG) Antibodies Used to Purify Peptides prior to LCMS: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990
Curated Info

5

Guo A (2009) CST Curation Set: 8645; Year: 2009; Biosample/Treatment: cell line, U266/Velcade; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG) Antibodies Used to Purify Peptides prior to LCMS: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990
Curated Info

6

Possemato A (2009) CST Curation Set: 6588; Year: 2009; Biosample/Treatment: cell line, U266/MG132; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG)
Curated Info

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