Tyr1173
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Phosphorylation Site Page:
Tyr1173 - INSR iso2 (human)

Site Information
FGMTRDIyETDYYRK   SwissProt Entrez-Gene
Predicted information:  Scansite
Orthologous residues: INSR (human): Y1185, INSR (rat): Y1186, INSR (mouse): Y1175
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447680

In vivo Characterization
Methods used to characterize site in vivo: [32P] bio-synthetic labeling (6), mass spectrometry (1, 2, 3, 4, 5), peptide sequencing (6), phospho-antibody (6), phosphoamino acid analysis (6)
Disease tissue studied: colorectal cancer (5), colorectal carcinoma (5)
Relevant cell line - cell type - tissue: 3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout] (6), CHO (fibroblast) [EphB1 (human), transfection] (6), HT-29 (intestinal) (5), NCI-H2228 (pulmonary) (1, 2), SW480 (intestinal) (4)

Controlled by
Kinases, in vitro: INSR (human) (6)
Treatments: insulin (6)



References

1

(2008) CST Curation Set: 5327; Year: 2008; Biosample/Treatment: cell line, NCI-H2228/untreated; Disease: non-small cell lung adenocarcinoma; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

2

(2008) CST Curation Set: 5328; Year: 2008; Biosample/Treatment: cell line, NCI-H2228/untreated; Disease: non-small cell lung adenocarcinoma; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

3

(2006) CST Curation Set: 2053; Year: 2006; Biosample/Treatment: tissue, larynx/-; Disease: laryngeal cancer; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

4

(2005) CST Curation Set: 573; Year: 2005; Biosample/Treatment: cell line, SW480/IGF1; Disease: colorectal carcinoma; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

5

(2004) CST Curation Set: 370; Year: 2004; Biosample/Treatment: cell line, HT-29/Serum starved & IGF; Disease: colorectal carcinoma; SILAC: -; Specificity of Antibody Used to Purify Peptides prior to MS2: anti-pTyr Antibody Used to Purify Peptides prior to MS2: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

6

Tennagels N, et al. (2001) Identification of Ser(1275) and Ser(1309) as autophosphorylation sites of the human insulin receptor in intact cells. Biochem Biophys Res Commun 282, 387-93
11401470   Curated Info

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