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Protein Page:
Src (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitylation
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
Src proto-oncogenic cytoplasmic tyrosine kinase of the SRC family. Highly expressed in certain fully differentiated cells such as neurons, platelets and macrophages. Phosphorylation of an activation loop tyrosine activates the enzyme; phosphorylation of a tyrosine in the C-terminus by Csk inhibits the enzyme. Two alternatively spliced isoforms have been described. Note: This description may include information from UniProtKB.
Protein type: Protein kinase, TK; Protein kinase, tyrosine (non-receptor); Oncoprotein; EC 2.7.10.2; Kinase, protein; TK group; Src family
Chromosomal Location of Human Ortholog: 20q12-q13
Cellular Component: caveola; cytoplasm; cytosol; extrinsic to internal side of plasma membrane; late endosome; lysosome; mitochondrial inner membrane; mitochondrion; plasma membrane
Molecular Function: enzyme binding; ephrin receptor binding; heme binding; hormone receptor binding; integrin binding; kinase activity; kinase binding; non-membrane spanning protein tyrosine kinase activity; phosphoprotein binding; protein binding; protein kinase activity; protein-tyrosine kinase activity; receptor binding; SH2 domain binding; SH3/SH2 adaptor activity
Biological Process: bone resorption; central nervous system development; ephrin receptor signaling pathway; epidermal growth factor receptor signaling pathway; estrogen receptor signaling pathway; innate immune response; integrin-mediated signaling pathway; leukocyte migration; negative regulation of apoptosis; negative regulation of caspase activity; negative regulation of focal adhesion formation; negative regulation of mitochondrial depolarization; negative regulation of protein homooligomerization; negative regulation of telomerase activity; negative regulation of telomere maintenance via telomerase; peptidyl-tyrosine phosphorylation; platelet activation; platelet-derived growth factor receptor signaling pathway; positive regulation of integrin activation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of protein kinase B signaling cascade; progesterone receptor signaling pathway; protein amino acid autophosphorylation; regulation of bone resorption; regulation of cell cycle; regulation of cell proliferation; regulation of cell-cell adhesion; regulation of vascular permeability; signal complex assembly; signal transduction; stimulatory C-type lectin receptor signaling pathway; stress fiber formation; T cell costimulation; transforming growth factor beta receptor signaling pathway; vascular endothelial growth factor receptor signaling pathway
Disease: Colorectal Cancer; Thrombocytopenia 6
Reference #:  P12931 (UniProtKB)
Alt. Names/Synonyms: ASV; c-SRC; p60-Src; pp60c-src; Proto-oncogene c-Src; Proto-oncogene tyrosine-protein kinase Src; protooncogene SRC, Rous sarcoma; SRC; SRC1; tyrosine kinase pp60c-src; tyrosine-protein kinase SRC-1; v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
Gene Symbols: SRC
Molecular weight: 59,835 Da
Basal Isoelectric point: 7.1  Predict pI for various phosphorylation states
CST Pathways:  Actin Dynamics  |  Adherens Junction Dynamics  |  Angiogenesis  |  ErbB/HER Signaling  |  GPCR Signaling to MAPKs  |  Growth And Differentiation Control by MAPKs  |  IL6 Signaling  |  Inhibition of Apoptosis  |  Microtubule Dynamics  |  Tyrosine Kinases & Substrates  |  Wnt/ß-Catenin Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
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Src

Protein Structure Not Found.
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Substrate Sequence Logo
Sequence Logo

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Sites Implicated In
carcinogenesis, altered: Y419‑p
cell adhesion, altered: Y419‑p
cell cycle regulation: Y419‑p
cell growth, altered: S75‑p, Y419‑p
cell growth, induced: Y419‑p
cell motility, altered: Y419‑p
cell motility, induced: Y419‑p
cell motility, inhibited: Y530‑p
cytoskeletal reorganization: S75‑p, Y419‑p
signaling pathway regulation: Y419‑p
transcription, induced: S70‑p
enzymatic activity, induced: S12‑p, S17‑p, S70‑p, S75‑p, Y419‑p
enzymatic activity, inhibited: S97‑p, Y530‑p
molecular association, regulation: S12‑p, Y419‑p, Y530‑p
protein conformation: Y530‑p
protein processing: Y530‑p
receptor internalization, induced: S70‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment



 LTP 

LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 HTP 

HTP: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
5 0 S12‑p KSKPKDAsQRRRsLE
10 48 S17‑p DAsQRRRsLEPAENV
0 7 A21 RRRsLEPAENVHGAG
1 2 S35‑p GGGAFPAsQtPsKPA
3 1 T37‑p GAFPAsQtPsKPAsA
1 1 S39‑p FPAsQtPsKPAsAdG
1 1 S43‑p QtPsKPAsAdGHRGP
0 1 D45‑ca PsKPAsAdGHRGPsA
2 0 G49 AsAdGHRGPsAAFAP
1 1 S51‑p AdGHRGPsAAFAPAA
0 1 K62‑ac APAAAEPkLFGGFNs
0 1 S69‑p kLFGGFNssDtVtsP
1 2 S70‑p LFGGFNssDtVtsPQ
0 2 T72‑p GGFNssDtVtsPQRA
0 2 T74‑p FNssDtVtsPQRAGP
8 24 S75‑p NssDtVtsPQRAGPL
0 2 Y93‑p VTTFVALyDYEsRTE
1 0 S97‑p VALyDYEsRTETDLs
0 1 T99 LyDYEsRTETDLsFK
0 1 T101 DYEsRTETDLsFKKG
0 3 S104‑p sRTETDLsFKKGERL
1 0 Y134 LSTGQTGYIPSNYVA
2 0 Y139 TGYIPSNYVAPSDSI
0 4 T182‑p FLVRESEttKGAyCL
0 15 T183‑p LVRESEttKGAyCLS
0 56 Y187‑p SEttKGAyCLSVSDF
8 22 Y216‑p KLDSGGFyITSRTQF
0 14 Y232 SLQQLVAYYSKHADG
0 1 T246‑p GLCHRLTtVCPtSKP
0 1 T250‑p RLTtVCPtSKPQTQG
0 1 K252 TtVCPtSKPQTQGLA
0 2 T304‑p IKTLKPGtMsPEAFL
0 2 S306‑p TLKPGtMsPEAFLQE
1 2 Y338‑p VVSEEPIyIVTEYMS
0 1 S348‑p TEYMSKGsLLDFLKG
329 3088 Y419‑p RLIEDNEytARQGAK
0 21 T420‑p LIEDNEytARQGAKF
1 534 Y439‑p TAPEAALyGRFTIKS
5 0 Y522‑p LQAFLEDyFTstEPQ
0 1 S525‑p FLEDyFTstEPQyQP
0 2 T526‑p LEDyFTstEPQyQPG
123 969 Y530‑p FTstEPQyQPGENL_
5473 : Phospho-Src (Ser17) Antibody
12432 : Phospho-Src (Ser17) (D7F2Q) Rabbit mAb
2101 : Phospho-Src Family (Tyr416) Antibody
6943 : Phospho-Src Family (Tyr416) (D49G4) Rabbit mAb
2105 : Phospho-Src (Tyr527) Antibody
  Src iso2  
S12 KSKPKDASQRRRSLE
S17 DASQRRRSLEPAENV
A21 RRRSLEPAENVHGAG
S35 GGGAFPASQTPSKPA
T37 GAFPASQTPSKPASA
S39 FPASQTPSKPASADG
S43 QTPSKPASADGHRGP
D45 PSKPASADGHRGPSA
G49 ASADGHRGPSAAFAP
S51 ADGHRGPSAAFAPAA
K62 APAAAEPKLFGGFNS
S69 KLFGGFNSSDTVTSP
S70 LFGGFNSSDTVTSPQ
T72 GGFNSSDTVTSPQRA
T74 FNSSDTVTSPQRAGP
S75 NSSDTVTSPQRAGPL
Y93 VTTFVALYDYESRTE
S97 VALYDYESRTETDLS
T99 LYDYESRTETDLSFK
T101 DYESRTETDLSFKKG
S104 SRTETDLSFKKGERL
Y140 LSTGQTGYIPSNYVA
Y145 TGYIPSNYVAPSDSI
T188 FLVRESETTKGAYCL
T189 LVRESETTKGAYCLS
Y193 SETTKGAYCLSVSDF
Y222 KLDSGGFYITSRTQF
Y238 SLQQLVAYYSKHADG
T252 GLCHRLTTVCPTSKP
T256 RLTTVCPTSKPQTQG
K258 TTVCPTSKPQTQGLA
T310 IKTLKPGTMSPEAFL
S312 TLKPGTMSPEAFLQE
Y344 VVSEEPIYIVTEYMS
S354 TEYMSKGSLLDFLKG
Y425 RLIEDNEYTARQGAK
T426 LIEDNEYTARQGAKF
Y445 TAPEAALYGRFTIKS
Y528 LQAFLEDYFTSTEPQ
S531 FLEDYFTSTEPQYQP
T532 LEDYFTSTEPQYQPG
Y536 FTSTEPQYQPGENL_
  mouse

► Hide Isoforms
 
S12 KSKPKDASQRRRsLE
S17‑p DASQRRRsLEPsENV
S21‑p RRRsLEPsENVHGAG
S34‑p AGGAFPAsQTPSKPA
T36 GAFPAsQTPSKPASA
S38 FPAsQTPSKPASADG
S42 QTPSKPASADGHRGP
D44 PSKPASADGHRGPSA
G48 ASADGHRGPSAAFVP
S50 ADGHRGPSAAFVPPA
K61 VPPAAEPKLFGGFNS
S68 KLFGGFNSSDtVTsP
S69 LFGGFNSSDtVTsPQ
T71‑p GGFNSSDtVTsPQRA
T73 FNSSDtVTsPQRAGP
S74‑p NSSDtVTsPQRAGPL
Y92‑p VTTFVALyDYESRtE
S96 VALyDYESRtEtDLs
T98‑p LyDYESRtEtDLsFK
T100‑p DYESRtEtDLsFKKG
S103‑p SRtEtDLsFKKGERL
Y139 LSTGQTGYIPSNYVA
Y144 TGYIPSNYVAPSDSI
T187 FLVRESETtKGAyCL
T188‑p LVRESETtKGAyCLS
Y192‑p SETtKGAyCLSVSDF
Y221‑p KLDSGGFyITSRTQF
Y237‑p SLQQLVAyYSKHADG
T251 GLCHRLTTVCPTSKP
T255 RLTTVCPTSKPQTQG
K257 TTVCPTSKPQTQGLA
T309 IKTLKPGTMSPEAFL
S311 TLKPGTMSPEAFLQE
Y343‑p VVSEEPIyIVTEYMN
S353 TEYMNKGSLLDFLKG
Y424‑p RLIEDNEytARQGAK
T425‑p LIEDNEytARQGAKF
Y444‑p TAPEAALyGRFTIKS
Y527‑p LQAFLEDyFTSTEPQ
S530 FLEDyFTSTEPQyQP
T531 LEDyFTSTEPQyQPG
Y535‑p FTSTEPQyQPGENL_
12432 : Phospho-Src (Ser17) (D7F2Q) Rabbit mAb
2101 : Phospho-Src Family (Tyr416) Antibody
6943 : Phospho-Src Family (Tyr416) (D49G4) Rabbit mAb
2105 : Phospho-Src (Tyr527) Antibody
  Src iso2  
S12‑p KSKPKDAsQRRRsLE
S17‑p DAsQRRRsLEPSENV
S21 RRRsLEPSENVHGAG
S34 AGGAFPASQTPSKPA
T36 GAFPASQTPSKPASA
S38 FPASQTPSKPASADG
S42 QTPSKPASADGHRGP
D44 PSKPASADGHRGPSA
G48 ASADGHRGPSAAFVP
S50 ADGHRGPSAAFVPPA
K61 VPPAAEPKLFGGFNS
S68 KLFGGFNSSDTVTsP
S69 LFGGFNSSDTVTsPQ
T71 GGFNSSDTVTsPQRA
T73 FNSSDTVTsPQRAGP
S74‑p NSSDTVTsPQRAGPL
Y92‑p VTTFVALyDYESRTE
S96 VALyDYESRTETDLS
T98 LyDYESRTETDLSFK
T100 DYESRTETDLSFKKG
S103 SRTETDLSFKKGERL
Y133‑p LSTGQTGyIPSNyVA
Y138‑p TGyIPSNyVAPSDSI
T181 FLVRESETTKGAYCL
T182 LVRESETTKGAYCLS
Y186 SETTKGAYCLSVSDF
Y215 KLDSGGFYITSRTQF
Y231 SLQQLVAYYSKHADG
T245 GLCHRLTTVCPTSKP
T249 RLTTVCPTSKPQTQG
K251 TTVCPTSKPQTQGLA
T303 IKTLKPGTMSPEAFL
S305 TLKPGTMSPEAFLQE
Y337 VVSEEPIYIVTEYMN
S347 TEYMNKGSLLDFLKG
Y418‑p RLIEDNEyTARQGAK
T419 LIEDNEyTARQGAKF
Y438‑p TAPEAALyGRFTIKS
Y521 LQAFLEDYFTSTEPQ
S524 FLEDYFTSTEPQyQP
T525 LEDYFTSTEPQyQPG
Y529‑p FTSTEPQyQPGENL_
12432 : Phospho-Src (Ser17) (D7F2Q) Rabbit mAb
  rat

 
S12 KSKPKDASQRRRsLE
S17‑p DASQRRRsLEPAENV
A21 RRRsLEPAENVHGAG
S34 AGGAFPASQTPSKPA
T36 GAFPASQTPSKPASA
S38 FPASQTPSKPASADG
S42 QTPSKPASADGHRGP
D44 PSKPASADGHRGPNA
G48 ASADGHRGPNAAFVP
N50 ADGHRGPNAAFVPPA
K62 PPAAAEPKLFGGFNS
S69 KLFGGFNSSDTVTsP
S70 LFGGFNSSDTVTsPQ
T72 GGFNSSDTVTsPQRA
T74 FNSSDTVTsPQRAGP
S75‑p NSSDTVTsPQRAGPL
Y93 VTTFVALYDYESRTE
S97 VALYDYESRTETDLS
T99 LYDYESRTETDLSFK
T101 DYESRTETDLSFKKG
S104 SRTETDLSFKKGERL
Y134 LSTGQTGYIPSNYVA
Y139 TGYIPSNYVAPSDSI
T182 FLVRESETTKGAyCL
T183 LVRESETTKGAyCLS
Y187‑p SETTKGAyCLSVSDF
Y216‑p KLDSGGFyITSRTQF
Y232‑p SLQQLVAyYSKHADG
T246 GLCHRLTTVCPTSkP
T250 RLTTVCPTSkPQTQG
K252‑ub TTVCPTSkPQTQGLA
T304 IKTLKPGTMSPEAFL
S306 TLKPGTMSPEAFLQE
Y338 VVSEEPIYIVTEYMN
S348 TEYMNKGSLLDFLKG
Y419‑p RLIEDNEyTARQGAK
T420 LIEDNEyTARQGAKF
Y439‑p TAPEAALyGRFTIKS
Y522 LQAFLEDYFTSTEPQ
S525 FLEDYFTSTEPQyQP
T526 LEDYFTSTEPQyQPG
Y530‑p FTSTEPQyQPGENL_
5473 : Phospho-Src (Ser17) Antibody
12432 : Phospho-Src (Ser17) (D7F2Q) Rabbit mAb
2101 : Phospho-Src Family (Tyr416) Antibody
6943 : Phospho-Src Family (Tyr416) (D49G4) Rabbit mAb
2105 : Phospho-Src (Tyr527) Antibody
  chicken

 
S12‑p KSKPKDPsQRRRsLE
S17‑p DPsQRRRsLEPPDST
P21 RRRsLEPPDSTHHGG
S32 HHGGFPASQtPNKTA
T34‑p GGFPASQtPNKTAAP
N36 FPASQtPNKTAAPDT
A40 QtPNKTAAPDTHRtP
D42 PNKTAAPDTHRtPsR
T46‑p AAPDTHRtPsRSFGT
S48‑p PDTHRtPsRSFGTVA
K59 GTVATEPKLFGGFNT
T66 KLFGGFNTSDTVTsP
S67 LFGGFNTSDTVTsPQ
T69 GGFNTSDTVTsPQRA
T71 FNTSDTVTsPQRAGA
S72‑p NTSDTVTsPQRAGAL
Y90 VTTFVALYDYESRTE
S94 VALYDYESRTETDLS
T96 LYDYESRTETDLSFK
T98 DYESRTETDLSFKKG
S101 SRTETDLSFKKGERL
Y131 LTTGQTGYIPSNYVA
Y136 TGYIPSNYVAPSDSI
T179 FLVRESETTKGAYCL
T180 LVRESETTKGAYCLS
Y184 SETTKGAYCLSVSDF
Y213 KLDSGGFYITSRTQF
Y229 SLQQLVAYYSKHADG
N243 GLCHRLTNVCPTSKP
T247 RLTNVCPTSKPQTQG
K249 TNVCPTSKPQTQGLA
T301 IKTLKPGTMSPEAFL
S303 TLKPGTMSPEAFLQE
Y335 VVSEEPIYIVTEYMS
S345 TEYMSKGSLLDFLKG
Y416‑p RLIEDNEyTARQGAK
T417 LIEDNEyTARQGAKF
Y436 TAPEAALYGRFTIKS
Y519‑p LQAFLEDyFTSTEPQ
S522 FLEDyFTSTEPQyQP
T523 LEDyFTSTEPQyQPG
Y527‑p FTSTEPQyQPGENL_
2101 : Phospho-Src Family (Tyr416) Antibody
2105 : Phospho-Src (Tyr527) Antibody
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