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Protein Page:
PDK1 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitylation
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
PDK1 an AGC kinase of the PKB family that contains a PH domain. Involved in a wide variety of processes including cell proliferation, differentiation and apoptosis. Autophosphorylation in the activation loop is necessary for activity. Phosphorylates and activates kinases including Akt, PKC isozymes, p70 S6K and RSK. Three alternatively-spliced isoforms have been described. Note: This description may include information from UniProtKB.
Protein type: Nuclear receptor co-regulator; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Kinase, protein; AGC group; PKB family
Chromosomal Location of Human Ortholog: 16p13.3
Cellular Component: cell projection; cytoplasm; cytosol; intracellular; nucleoplasm; plasma membrane
Molecular Function: 3-phosphoinositide-dependent protein kinase activity; kinase activity; phospholipase activator activity; phospholipase binding; protein binding; protein serine/threonine kinase activity
Biological Process: actin cytoskeleton organization and biogenesis; activation of protein kinase B; calcium-mediated signaling; cell migration; cellular response to insulin stimulus; epidermal growth factor receptor signaling pathway; negative regulation of protein kinase activity; negative regulation of transforming growth factor beta receptor signaling pathway; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; phosphoinositide-mediated signaling; platelet activation; positive regulation of release of sequestered calcium ion into cytosol; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of I-kappaB kinase/NF-kappaB cascade; stimulatory C-type lectin receptor signaling pathway; T cell costimulation; T cell receptor signaling pathway
Reference #:  O15530 (UniProtKB)
Alt. Names/Synonyms: 3-phosphoinositide dependent protein kinase-1; 3-phosphoinositide-dependent protein kinase 1; hPDK1; MGC20087; MGC35290; PDK1; PDPK1; PkB kinase like gene 1; PkB-like 1; PRO0461; protein kinase
Gene Symbols: PDPK1
Molecular weight: 63,152 Da
Basal Isoelectric point: 6.95  Predict pI for various phosphorylation states
CST Pathways:  Inhibition of Apoptosis  |  Insulin Receptor Signaling  |  mTOR Signaling  |  NF-kB Signaling  |  PI3K/Akt Signaling  |  Protein Kinase C Signaling  |  T Cell Receptor Signaling  |  Translation: eIF4E and p70S6K
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
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PDK1

Protein Structure Not Found.
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Substrate Sequence Logo
Sequence Logo

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Sites Implicated In
apoptosis, altered: T354‑p, Y373‑p, Y376‑p, S394‑p, S398‑p
cell growth, altered: S394‑p, S398‑p
cell growth, induced: S241‑p
cell growth, inhibited: T354‑p
signaling pathway regulation: T354‑p
transcription, altered: Y9‑p, S396‑p
enzymatic activity, induced: Y9‑p, S241‑p, Y373‑p, Y376‑p, T513‑p
enzymatic activity, inhibited: T354‑p
intracellular localization: S396‑p
molecular association, regulation: Y9‑p, S241‑p, T354‑p, T513‑p
phosphorylation: Y9‑p
protein degradation: Y9‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment



 LTP 

LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 HTP 

HTP: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 1 R3 _____MARTTSQLyD
5 7 Y9‑p ARTTSQLyDAVPIQS
0 1 S22‑p QSSVVLCsCPsPSMV
1 2 S25‑p VVLCsCPsPSMVRTQ
0 2 T33‑p PSMVRTQtESstPPG
0 1 S36‑p VRTQtESstPPGIPG
0 21 T37‑p RTQtESstPPGIPGG
0 2 P38 TQtESstPPGIPGGS
0 1 P49 PGGSRQGPAMDGTAA
0 1 T54 QGPAMDGTAAEPRPG
0 1 A55 GPAMDGTAAEPRPGA
0 1 A62 AAEPRPGAGSLQHAQ
0 1 T95 LGEGSFSTVVLAREL
0 2 K123‑ub RHIIKENkVPYVTRE
0 1 T148‑p FFVKLYFtFQDDEKL
0 1 Y156‑p FQDDEKLyFGLsyAK
1 1 S160‑p EKLyFGLsyAKNGEL
0 1 Y161‑p KLyFGLsyAKNGELL
0 1 S176‑p KYIRKIGsFDEtCTR
0 1 T180‑p KIGsFDEtCTRFYTA
0 2 S231‑p FGTAKVLsPEsKQAR
0 1 S234‑p AKVLsPEsKQARANs
50 318 S241‑p sKQARANsFVGtAQy
0 23 T245‑p RANsFVGtAQyVSPE
0 4 Y248‑p sFVGtAQyVSPELLt
0 1 T255‑p yVSPELLtEKSACKS
0 1 K257 SPELLtEKSACKSSD
0 5 Y288‑p PFRAGNEyLIFQKII
0 1 K296 LIFQKIIKLEYDFPE
0 9 K304‑ac LEYDFPEkFFPKARD
0 1 K304‑ub LEYDFPEkFFPKARD
1 0 T354‑p WENLHQQtPPKLTAY
0 1 S366‑p TAYLPAMsEDDEDCy
9 1 Y373‑p sEDDEDCyGNyDNLL
8 1 Y376‑p DEDCyGNyDNLLSQF
2 1 S393‑p MQVSSSSssHsLsAS
2 0 S394‑p QVSSSSssHsLsASD
4 1 S396‑p SSSSssHsLsASDTG
2 0 S398‑p SSssHsLsASDTGLP
1 1 S410‑p GLPQRSGsNIEQYIH
0 10 K441‑ub EKRLLLEkQAGGNPW
0 1 Y485‑p LTEGPHLyyVDPVNK
0 1 Y486‑p TEGPHLyyVDPVNKV
1 1 S501‑p LKGEIPWsQELRPEA
5 3 T513‑p PEAKNFKtFFVHTPN
1 0 S529 TYYLMDPSGNAHkWC
0 1 K534‑ac DPSGNAHkWCRKIQE
3061 : Phospho-PDK1 (Ser241) Antibody
3438 : Phospho-PDK1 (Ser241) (C49H2) Rabbit mAb
  PDK1 iso2  
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
T4 ____MDGTAAEPRPG
A5 ___MDGTAAEPRPGA
A12 AAEPRPGAGSLQHAQ
T45 LGEGSFSTVVLAREL
K73 RHIIKENKVPYVTRE
T98 FFVKLYFTFQDDEKL
Y106 FQDDEKLYFGLSYAK
S110 EKLYFGLSYAKNGEL
Y111 KLYFGLSYAKNGELL
S126 KYIRKIGSFDETCTR
T130 KIGSFDETCTRFYTA
S181 FGTAKVLSPESKQAR
S184 AKVLSPESKQARANS
S191 SKQARANSFVGTAQY
T195 RANSFVGTAQYVSPE
Y198 SFVGTAQYVSPELLT
T205 YVSPELLTEKSACKS
K207 SPELLTEKSACKSSD
Y238 PFRAGNEYLIFQKII
K246 LIFQKIIKLEYDFPE
K254 LEYDFPEKFFPKARD
K254 LEYDFPEKFFPKARD
T304 WENLHQQTPPKLTAY
S316 TAYLPAMSEDDEDCY
Y323 SEDDEDCYGNYDNLL
Y326 DEDCYGNYDNLLSQF
S343 MQVSSSSSSHSLSAS
S344 QVSSSSSSHSLSASD
S346 SSSSSSHSLSASDTG
S348 SSSSHSLSASDTGLP
S360 GLPQRSGSNIEQYIH
K391 EKRLLLEKQAGGNPW
Y435 LTEGPHLYYVDPVNK
Y436 TEGPHLYYVDPVNKV
S451 LKGEIPWSQELRPEA
T463 PEAKNFKTFFVHTPN
S479 TYYLMDPSGNAHKWC
K484 DPSGNAHKWCRKIQE
  PDK1 iso4  
R3 _____MARTTSQLYD
Y9 ARTTSQLYDAVPIQS
S22 QSSVVLCSCPSPSMV
S25 VVLCSCPSPSMVRTQ
T33 PSMVRTQTESStPPG
S36 VRTQTESStPPGIPG
T37‑p RTQTESStPPGIPGG
P38 TQTESStPPGIPGGS
P49 PGGSRQGPAMDGTAA
T54 QGPAMDGTAAEPRPG
A55 GPAMDGTAAEPRPGA
A62 AAEPRPGAGSLQHAQ
T95 LGEGSFSTVVLAREL
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
S114‑p EYATRANsFVGTAQY
T118 RANsFVGTAQYVSPE
Y121 sFVGTAQYVSPELLT
T128 YVSPELLTEKSACKS
K130 SPELLTEKSACKSSD
Y161 PFRAGNEYLIFQKII
K169 LIFQKIIKLEYDFPE
K177 LEYDFPEKFFPKARD
K177 LEYDFPEKFFPKARD
T227 WENLHQQTPPKLTAY
S239 TAYLPAMSEDDEDCY
Y246 SEDDEDCYGNYDNLL
Y249 DEDCYGNYDNLLSQF
S266 MQVSSSSSSHSLSAS
S267 QVSSSSSSHSLSASD
S269 SSSSSSHSLSASDTG
S271 SSSSHSLSASDTGLP
S283 GLPQRSGSNIEQYIH
K314‑ub EKRLLLEkQAGGNPW
Y358 LTEGPHLYYVDPVNK
Y359 TEGPHLYYVDPVNKV
S374 LKGEIPWSQELRPEA
T386 PEAKNFKTFFVHTPN
S402 TYYLMDPSGNAHkWC
K407‑ac DPSGNAHkWCRKIQE
  PDK1 iso5  
R3 _____MARTTSQLYD
Y9 ARTTSQLYDAVPIQS
S22 QSSVVLCSCPSPSMV
S25 VVLCSCPSPSMVRTQ
T33 PSMVRTQTESSTPPG
S36 VRTQTESSTPPGIPG
T37 RTQTESSTPPGIPGG
P38 TQTESSTPPGIPGGS
P49 PGGSRQGPAMDGTAA
T54 QGPAMDGTAAEPRPG
A55 GPAMDGTAAEPRPGA
A62 AAEPRPGAGSLQHAQ
T95 LGEGSFSTVVLAREL
K123 RHIIKENKVPYVTRE
T148 FFVKLYFTFQDDEKL
Y156 FQDDEKLYFGLSYAK
S160 EKLYFGLSYAKNGEL
Y161 KLYFGLSYAKNGELL
S176 KYIRKIGSFDETCTR
T180 KIGSFDETCTRFYTA
S231 FGTAKVLSPESKQAR
S234 AKVLSPESKQARANS
S241 SKQARANSFVGTAQY
T245 RANSFVGTAQYVSPE
Y248 SFVGTAQYVSPELLT
T255 YVSPELLTEKSACKS
K257 SPELLTEKSACKSSD
Y288 PFRAGNEYLIFQKII
K296 LIFQKIIKLEYDFPE
K304 LEYDFPEKFFPKARD
K304 LEYDFPEKFFPKARD
T354 WENLHQQTPPKLTAY
S366 TAYLPAMSEDDEDCY
Y373 SEDDEDCYGNYDNLL
Y376 DEDCYGNYDNLLSQF
S393 MQVSSSSSSHSLSAS
S394 QVSSSSSSHSLSASD
S396 SSSSSSHSLSASDTG
S398 SSSSHSLSASDTGLP
S410 GLPQRSGSNIEQYIH
K441 EKRLLLEKQAGGNPC
- gap
- gap
- gap
- gap
- gap
- gap
  mouse

 
R3‑m1 _____MArTTSQLYD
Y9 ArTTSQLYDAVPIQS
S22 QSSVVLCSCPSPSMV
S25 VVLCSCPSPSMVRSQ
T33 PSMVRSQTEPGSsPG
G36 VRSQTEPGSsPGIPS
S37 RSQTEPGSsPGIPSG
S38‑p SQTEPGSsPGIPSGV
S50‑p SGVSRQGsTMDGttA
T55‑p QGsTMDGttAEARPS
T56‑p GsTMDGttAEARPSt
T63‑p tAEARPStNPLQQHP
T98‑p LGEGSFStVVLAREL
K126‑ub RHIIKENkVPYVTRE
T151 FFVKLYFTFQDDEKL
Y159 FQDDEKLYFGLsYAK
S163‑p EKLYFGLsYAKNGEL
Y164 KLYFGLsYAKNGELL
S179 KYIRKIGSFDETCTR
T183 KIGSFDETCTRFYTA
S234‑p FGTAKVLsPESKQAR
S237 AKVLsPESKQARANs
S244‑p SKQARANsFVGtAQy
T248‑p RANsFVGtAQyVSPE
Y251‑p sFVGtAQyVSPELLT
T258 yVSPELLTEkSACKS
K260‑ub SPELLTEkSACKSSD
Y291 PFRAGNEYLIFQKII
K299‑ub LIFQKIIkLEYHFPE
K307‑ac LEYHFPEkFFPKARD
K307 LEYHFPEKFFPKARD
T357 WENLHQQTPPKLTAY
S369 TAYLPAMSEDDEDCY
Y376 SEDDEDCYGNYDNLL
Y379 DEDCYGNYDNLLSQF
S396 MQVSSSSSSHsLSTV
S397 QVSSSSSSHsLSTVE
S399‑p SSSSSSHsLSTVETS
S401 SSSSHsLSTVETSLP
S413 SLPQRSGSNIEQYIH
K444 EKRLLLEKQAGGNPW
Y488 LTEGPHLYYVDPVNK
Y489 TEGPHLYYVDPVNKV
S504‑p LKGEIPWsQELRPEA
T516‑p PEAKNFKtFFVHTPN
S532‑p TYYLMDPsGNAHKWC
K537 DPsGNAHKWCRKIQE
3061 : Phospho-PDK1 (Ser241) Antibody
3438 : Phospho-PDK1 (Ser241) (C49H2) Rabbit mAb
  rat

 
R3 _____MARTTSQLyD
Y9‑p ARTTSQLyDAVPIQS
S22 QSSVVLCSCPSPSMV
S25 VVLCSCPSPSMVRSQ
T33 PSMVRSQTEPSSSPG
S36 VRSQTEPSSSPGIPS
S37 RSQTEPSSSPGIPSG
S38 SQTEPSSSPGIPSGV
S50 SGVSRQGSTMDGTTA
T55 QGSTMDGTTAEARPS
T56 GSTMDGTTAEARPST
T63 TAEARPSTNPLQQHP
T98 LGEGSFSTVVLAREL
K126 RHIIKENKVPYVTRE
T151 FFVKLYFTFQDDEKL
Y159 FQDDEKLYFGLSYAK
S163 EKLYFGLSYAKNGEL
Y164 KLYFGLSYAKNGELL
S179 KYIRKIGSFDETCTR
T183 KIGSFDETCTRFYTA
S234 FGTAKVLSPDSKQAR
S237 AKVLSPDSKQARANs
S244‑p SKQARANsFVGTAQY
T248 RANsFVGTAQYVSPE
Y251 sFVGTAQYVSPELLT
T258 YVSPELLTEKSACKS
K260 SPELLTEKSACKSSD
Y291 PFRAGNEYLIFQKII
K299 LIFQKIIKLEYDFPE
K307‑ac LEYDFPEkFFPKARD
K307 LEYDFPEKFFPKARD
T357 WENLHQQTPPKLTAY
S369 TAYLPAMSEDDEDCy
Y376‑p SEDDEDCyGNyDNLL
Y379‑p DEDCyGNyDNLLSQF
S396 MQVSSSSSSHSLSAV
S397 QVSSSSSSHSLSAVD
S399 SSSSSSHSLSAVDAS
S401 SSSSHSLSAVDASLP
S413 SLPQRSGSNIEQYIH
K444 EKRLLLEKQAGGNPW
Y488 LTEGPHLYYVDPVNK
Y489 TEGPHLYYVDPVNKV
S504 LKGEIPWSQELRPEA
T516 PEAKNFKTFFVHTPN
S532 TYYLMDPSGNAHKWC
K537 DPSGNAHKWCRKIQE
3061 : Phospho-PDK1 (Ser241) Antibody
3438 : Phospho-PDK1 (Ser241) (C49H2) Rabbit mAb
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