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Protein Page:
GSK3B (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitylation
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
GSK3B a proline-directed protein kinase of the GSK family. Phosphorylates and inactivates glycogen synthase. Participates in the Wnt signaling pathway. Involved in energy metabolism, neuronal cell development, and body pattern formation Note: This description may include information from UniProtKB.
Protein type: Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.26; Kinase, protein; EC 2.7.11.1; CMGC group; GSK family; GSK subfamily
Chromosomal Location of Human Ortholog: 3q13.3
Cellular Component: beta-catenin destruction complex; centrosome; cytoplasm; cytosol; nucleus; plasma membrane
Molecular Function: beta-catenin binding; kinase activity; NF-kappaB binding; p53 binding; protein binding; protein kinase activity; protein kinase binding; protein serine/threonine kinase activity; tau-protein kinase activity; ubiquitin protein ligase binding
Biological Process: circadian rhythm; epithelial to mesenchymal transition; ER overload response; glycogen metabolic process; hippocampus development; negative regulation of apoptosis; negative regulation of glycogen biosynthetic process; negative regulation of NFAT protein import into nucleus; negative regulation of protein binding; negative regulation of protein complex assembly; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; positive regulation of cell-matrix adhesion; positive regulation of GTPase activity; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein binding; positive regulation of protein catabolic process; positive regulation of protein complex assembly; positive regulation of protein export from nucleus; proteasomal ubiquitin-dependent protein catabolic process; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of microtubule-based process; Wnt receptor signaling pathway; Wnt receptor signaling pathway through beta-catenin
Reference #:  P49841 (UniProtKB)
Alt. Names/Synonyms: glycogen synthase kinase 3 beta; Glycogen synthase kinase-3 beta; GSK-3 beta; GSK3B; GSK3beta isoform
Gene Symbols: GSK3B
Molecular weight: 46,744 Da
Basal Isoelectric point: 8.98  Predict pI for various phosphorylation states
CST Pathways:  Adherens Junction Dynamics  |  Alzheimer's Disease  |  B Cell Receptor Signaling  |  ErbB/HER Signaling  |  ESC Pluripotency and Differentiation  |  G1/S Checkpoint  |  Growth And Differentiation Control by MAPKs  |  Hedgehog Signaling  |  Inhibition of Apoptosis  |  Insulin Receptor Signaling  |  Microtubule Dynamics  |  mTOR Signaling  |  NF-kB Signaling  |  PI3K/Akt Signaling  |  Translation: eIF2  |  Translation: eIF4E and p70S6K  |  Wnt/ß-Catenin Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

GSK3B

Protein Structure Not Found.
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Substrate Sequence Logo
Sequence Logo

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Sites Implicated In
apoptosis, altered: S9‑p, Y216‑p
apoptosis, induced: Y216‑p
apoptosis, inhibited: S9‑p
cell adhesion, altered: S9‑p
cell differentiation, induced: S9‑p
cell growth, altered: S9‑p, Y216‑p
cell motility, altered: S9‑p
cytoskeletal reorganization: S9‑p
transcription, altered: S9‑p, Y216‑p
transcription, induced: Y216‑p
activity, inhibited: S9‑p
enzymatic activity, induced: S147‑p, Y216‑p
enzymatic activity, inhibited: S9‑p
intracellular localization: S9‑p, Y216‑p
molecular association, regulation: Y216‑p
phosphorylation: S9‑p
protein conformation: Y216‑p
protein degradation: S9‑p
protein stabilization: Y216‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment



 LTP 

LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 HTP 

HTP: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 22 T7‑p _MSGRPRttsFAEsC
0 22 T8‑p MSGRPRttsFAEsCK
389 254 S9‑p SGRPRttsFAEsCKP
0 5 S13‑p RttsFAEsCKPVQQP
0 10 S21‑p CKPVQQPsAFGsMKV
0 75 S25‑p QQPsAFGsMKVSRDK
0 1 S35‑p VSRDKDGsKVTTVVA
2 1 T43‑p KVTTVVAtPGQGPDR
0 1 S66‑p TKVIGNGsFGVVyQA
0 9 Y71‑p NGsFGVVyQAKLCDS
1 0 S147‑p YRVARHYsRAKQTLP
2 0 K205‑ac LCDFGSAkQLVRGEP
0 8 K205‑ub LCDFGSAkQLVRGEP
0 1265 S215‑p VRGEPNVsyICsRyy
71 4263 Y216‑p RGEPNVsyICsRyyR
1 1127 S219‑p PNVsyICsRyyRAPE
0 109 Y221‑p VsyICsRyyRAPELI
0 6 Y222‑p syICsRyyRAPELIF
0 3 K292‑ub NPNYTEFkFPQIKAH
1 0 K292‑sm NPNYTEFkFPQIKAH
1 0 T356 KLPNGRDTPALFNFT
4 9 S389‑p ARIQAAAstPtNAtA
5 25 T390‑p RIQAAAstPtNAtAA
0 1 T392‑p QAAAstPtNAtAASD
0 6 T395‑p AstPtNAtAASDANt
0 2 T402‑p tAASDANtGDRGQTN
0 1 S417‑p NAASASAsNSt____
0 1 T420‑p SASAsNSt_______
5558 : Phospho-GSK-3beta (Ser9) (D85E12) XP(TM) Rabbit mAb
8466 : Phospho-GSK-3beta (Ser9) (D85E12) XP(R) Rabbit mAb (PE Conjugate)
8566 : Phospho-GSK-3-alpha/beta (Ser21/9) (D17D2) Rabbit mAb
9322 : Phospho-GSK-3-beta (Ser9) (D3A4) Rabbit mAb
9323 : Phospho-GSK-3beta (Ser9) (5B3) Rabbit mAb
9325 : GSK-3 beta-Tag Antibody
9327 : Phospho-GSK-3alpha/beta (Ser21/9) (37F11) Rabbit mAb (GSK-3alpha Preferred)
9331 : Phospho-GSK-3alpha/beta (Ser21/9) Antibody
9336 : Phospho-GSK-3beta (Ser9) Antibody
14026 : Phospho-GSK-3beta (Ser9) (D85E12) XP(R) Rabbit mAb (Alexa Fluor(R) 488 Conjugate)
14310 : Phospho-GSK-3beta (Ser9) (D85E12) XP(R) Rabbit mAb (Pacific Blue(TM) Conjugate)
14332 : Phospho-GSK-3beta (Ser9) (D85E12) XP(R) Rabbit mAb (Alexa Fluor(R) 647 Conjugate)
14630 : Phospho-GSK-3beta (Ser9) (D2Y9Y) Mouse mAb
3548 : Phospho GSK-3beta(Thr390) Antibody
  GSK3B iso2  
T7 _MSGRPRTTsFAESC
T8 MSGRPRTTsFAESCK
S9‑p SGRPRTTsFAESCKP
S13 RTTsFAESCKPVQQP
S21 CKPVQQPSAFGSMKV
S25 QQPSAFGSMKVSRDK
S35 VSRDKDGSKVTTVVA
T43 KVTTVVATPGQGPDR
S66 TKVIGNGSFGVVYQA
Y71 NGSFGVVYQAKLCDS
S147 YRVARHYSRAKQTLP
K205 LCDFGSAKQLVRGEP
K205 LCDFGSAKQLVRGEP
S215 VRGEPNVSyICSRYY
Y216‑p RGEPNVSyICSRYYR
S219 PNVSyICSRYYRAPE
Y221 VSyICSRYYRAPELI
Y222 SyICSRYYRAPELIF
K292 NPNYTEFKFPQIKAH
K292 NPNYTEFKFPQIKAH
T369 KLPNGRDTPALFNFT
S402 ARIQAAASTPTNATA
T403 RIQAAASTPTNATAA
T405 QAAASTPTNATAASD
T408 ASTPTNATAASDANT
T415 TAASDANTGDRGQTN
S430 NAASASASNST____
T433 SASASNST_______
8466 : Phospho-GSK-3beta (Ser9) (D85E12) XP(R) Rabbit mAb (PE Conjugate)
8566 : Phospho-GSK-3-alpha/beta (Ser21/9) (D17D2) Rabbit mAb
9325 : GSK-3 beta-Tag Antibody
14026 : Phospho-GSK-3beta (Ser9) (D85E12) XP(R) Rabbit mAb (Alexa Fluor(R) 488 Conjugate)
14310 : Phospho-GSK-3beta (Ser9) (D85E12) XP(R) Rabbit mAb (Pacific Blue(TM) Conjugate)
14332 : Phospho-GSK-3beta (Ser9) (D85E12) XP(R) Rabbit mAb (Alexa Fluor(R) 647 Conjugate)
  mouse

 
T7‑p _MSGRPRttsFAEsC
T8‑p MSGRPRttsFAEsCK
S9‑p SGRPRttsFAEsCKP
S13‑p RttsFAEsCKPVQQP
S21‑p CKPVQQPsAFGsMKV
S25‑p QQPsAFGsMKVSRDK
S35 VSRDKDGSKVTTVVA
T43 KVTTVVATPGQGPDR
S66 TKVIGNGSFGVVYQA
Y71 NGSFGVVYQAKLCDS
S147 YRVARHYSRAKQTLP
K205 LCDFGSAKQLVRGEP
K205‑ub LCDFGSAkQLVRGEP
S215‑p VRGEPNVsyICsRYY
Y216‑p RGEPNVsyICsRYYR
S219‑p PNVsyICsRYYRAPE
Y221 VsyICsRYYRAPELI
Y222 syICsRYYRAPELIF
K292‑ub NPNYTEFkFPQIKAH
K292 NPNYTEFKFPQIKAH
T356‑p KLPNGRDtPALFNFT
S389‑p ARIQAAAsPPANAtA
P390 RIQAAAsPPANAtAA
A392 QAAAsPPANAtAASD
T395‑p AsPPANAtAASDTNA
A402 tAASDTNAGDRGQTN
S417 NAASASASNST____
T420 SASASNST_______
5558 : Phospho-GSK-3beta (Ser9) (D85E12) XP(TM) Rabbit mAb
8466 : Phospho-GSK-3beta (Ser9) (D85E12) XP(R) Rabbit mAb (PE Conjugate)
8566 : Phospho-GSK-3-alpha/beta (Ser21/9) (D17D2) Rabbit mAb
9322 : Phospho-GSK-3-beta (Ser9) (D3A4) Rabbit mAb
9323 : Phospho-GSK-3beta (Ser9) (5B3) Rabbit mAb
9325 : GSK-3 beta-Tag Antibody
9327 : Phospho-GSK-3alpha/beta (Ser21/9) (37F11) Rabbit mAb (GSK-3alpha Preferred)
9331 : Phospho-GSK-3alpha/beta (Ser21/9) Antibody
9336 : Phospho-GSK-3beta (Ser9) Antibody
14026 : Phospho-GSK-3beta (Ser9) (D85E12) XP(R) Rabbit mAb (Alexa Fluor(R) 488 Conjugate)
14310 : Phospho-GSK-3beta (Ser9) (D85E12) XP(R) Rabbit mAb (Pacific Blue(TM) Conjugate)
14332 : Phospho-GSK-3beta (Ser9) (D85E12) XP(R) Rabbit mAb (Alexa Fluor(R) 647 Conjugate)
14630 : Phospho-GSK-3beta (Ser9) (D2Y9Y) Mouse mAb
  rat

 
T7 _MSGRPRTTsFAESC
T8 MSGRPRTTsFAESCK
S9‑p SGRPRTTsFAESCKP
S13 RTTsFAESCKPVQQP
S21 CKPVQQPSAFGSMKV
S25 QQPSAFGSMKVSRDK
S35 VSRDKDGSKVTTVVA
T43 KVTTVVATPGQGPDR
S66 TKVIGNGSFGVVyQA
Y71‑p NGSFGVVyQAKLCDS
S147 YRVARHYSRAKQTLP
K205‑ac LCDFGSAkQLVRGEP
K205 LCDFGSAKQLVRGEP
S215‑p VRGEPNVsyICsRyY
Y216‑p RGEPNVsyICsRyYR
S219‑p PNVsyICsRyYRAPE
Y221‑p VsyICsRyYRAPELI
Y222 syICsRyYRAPELIF
K292 NPNYTEFKFPQIKAH
K292 NPNYTEFKFPQIKAH
T356 KLPNGRDTPALFNFT
S389‑p ARIQAAAsPPANATA
P390 RIQAAAsPPANATAA
A392 QAAAsPPANATAASD
T395 AsPPANATAASDTNA
A402 TAASDTNAGDRGQTN
S417 NAASASASNST____
T420 SASASNST_______
5558 : Phospho-GSK-3beta (Ser9) (D85E12) XP(TM) Rabbit mAb
8466 : Phospho-GSK-3beta (Ser9) (D85E12) XP(R) Rabbit mAb (PE Conjugate)
8566 : Phospho-GSK-3-alpha/beta (Ser21/9) (D17D2) Rabbit mAb
9322 : Phospho-GSK-3-beta (Ser9) (D3A4) Rabbit mAb
9323 : Phospho-GSK-3beta (Ser9) (5B3) Rabbit mAb
9325 : GSK-3 beta-Tag Antibody
9327 : Phospho-GSK-3alpha/beta (Ser21/9) (37F11) Rabbit mAb (GSK-3alpha Preferred)
9331 : Phospho-GSK-3alpha/beta (Ser21/9) Antibody
9336 : Phospho-GSK-3beta (Ser9) Antibody
14026 : Phospho-GSK-3beta (Ser9) (D85E12) XP(R) Rabbit mAb (Alexa Fluor(R) 488 Conjugate)
14310 : Phospho-GSK-3beta (Ser9) (D85E12) XP(R) Rabbit mAb (Pacific Blue(TM) Conjugate)
14332 : Phospho-GSK-3beta (Ser9) (D85E12) XP(R) Rabbit mAb (Alexa Fluor(R) 647 Conjugate)
14630 : Phospho-GSK-3beta (Ser9) (D2Y9Y) Mouse mAb
  rabbit

 
- gap
T2 ______XTSFAESXK
S3 _____XTSFAESXKP
S7 _XTSFAESXKPVQQP
S15 XKPVQQPSAFGS___
S19 QQPSAFGS_______
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
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