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Protein Page:
IKKB (mouse)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitylation
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
IKKB a kinase of the IKK family. Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Preferentially found as a heterodimer with IKK-alpha but also as an homodimer. Note: This description may include information from UniProtKB.
Protein type: Kinase, protein; Protein kinase, Other; EC 2.7.11.10; Protein kinase, Ser/Thr (non-receptor); Other group; IKK family
Cellular Component: cytoplasm; cytosol; IkappaB kinase complex; internal side of plasma membrane; intracellular membrane-bound organelle
Molecular Function: ATP binding; IkappaB kinase activity; protein binding; protein heterodimerization activity; protein homodimerization activity; protein kinase activity; protein kinase binding; protein serine/threonine kinase activity; receptor signaling protein serine/threonine kinase activity
Biological Process: activation of NF-kappaB transcription factor; B cell homeostasis; cortical actin cytoskeleton organization and biogenesis; I-kappaB kinase/NF-kappaB cascade; I-kappaB phosphorylation; neurite development; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; regulation of phosphorylation; serine phosphorylation of STAT protein; tumor necrosis factor-mediated signaling pathway
Reference #:  O88351 (UniProtKB)
Alt. Names/Synonyms: AI132552; I-kappa-B kinase 2; I-kappa-B-kinase beta; IkappaB kinase; IkBKB; IKK-2; IKK-B; IKK-beta; IKK2; IKK[b]; Ikkb; IKKbeta; inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta; inhibitor of kappaB kinase beta; Inhibitor of nuclear factor kappa-B kinase subunit beta; NFKBIKB; Nuclear factor NF-kappa-B inhibitor kinase beta; OTTMUSP00000022456
Gene Symbols: Ikbkb
Molecular weight: 86,690 Da
Basal Isoelectric point: 6.03  Predict pI for various phosphorylation states
CST Pathways:  Apoptosis Regulation  |  B Cell Receptor Signaling  |  Death Receptor Signaling  |  Inhibition of Apoptosis  |  Insulin Receptor Signaling  |  NF-kB Signaling  |  T Cell Receptor Signaling  |  Toll-Like Receptor Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

IKKB

Protein Structure Not Found.
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Substrate Sequence Logo
Sequence Logo

STRING  |  Reactome  |  BioGPS  |  Scansite  |  KinBase  |  Pfam  |  ENZYME  |  Phospho.ELM  |  NetworKIN  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene


Sites Implicated In
apoptosis, altered: S181‑p
cell differentiation, induced: S177‑p, S181‑p, Y188‑p, Y199‑p
enzymatic activity, induced: S177‑p, S181‑p, Y188‑p, Y199‑p
molecular association, regulation: S181‑p, S679‑p, S682‑p, S689‑p, S692‑p, S697‑p, S701‑p, S705‑p
phosphorylation: S181‑p, S679‑p, S682‑p, S689‑p, S692‑p, S697‑p, S701‑p, S705‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment



 LTP 

LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 HTP 

HTP: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       mouse

 
0 2 S4 ____MSWSPSLPTQT
0 1 S6 __MSWSPSLPTQTCG
0 1 T23 EMKERLGTGGFGNVI
0 1 R106 CQGGDLRRYLNQFEN
1 0 K147 RIIHRDLKPENIVLQ
1 0 K163 GEKRLIHKIIDLGYA
1 0 K171 IIDLGYAKELDQGsL
30 0 S177‑p AKELDQGsLCTsFVG
0 1 T180 LDQGsLCTsFVGTLQ
1 0 T180 LDQGsLCTsFVGTLQ
59 2 S181‑p DQGsLCTsFVGTLQy
5 0 Y188‑p sFVGTLQyLAPELLE
5 1 Y199‑p ELLEQQKyTVTVDYW
0 1 S239 HSKVRQKSEVDIVVS
0 1 S246 SEVDIVVSEDLNGAV
0 1 S256 LNGAVKFSSSLPFPN
0 1 S257 NGAVKFSSSLPFPNN
0 1 S258 GAVKFSSSLPFPNNL
0 1 F261 KFSSSLPFPNNLNSV
0 1 S267 PFPNNLNSVLAERLE
0 1 T324 MVTGTVHTYPVTEDE
0 1 Y325 VTGTVHTYPVTEDES
0 1 S332 YPVTEDESLQSLKTR
0 1 S335 TEDESLQSLKTRIQE
0 1 T344 KTRIQENTGILETDQ
0 1 T368 LLPDKPATQCISDSK
0 1 S393 LVFLLDNSKINYETQ
0 1 N396 LLDNSKINYETQITP
0 1 Y397 LDNSKINYETQITPR
0 1 T399 NSKINYETQITPRPP
0 1 T402 INYETQITPRPPPES
0 2 S409 TPRPPPESVSCILQE
0 2 S411 RPPPESVSCILQEPK
1 0 K418 SCILQEPKRNLSFFQ
0 1 S466‑p LRNNSCLsKMKNAMA
0 1 A471 CLsKMKNAMASTAQQ
0 1 S474 KMKNAMASTAQQLKA
0 1 A476 KNAMASTAQQLKAKL
0 1 T488 AKLDFFKTSIQIDLE
0 1 S489 KLDFFKTSIQIDLEK
0 1 K498‑ub QIDLEKYkEQTEFGI
0 1 S507 QTEFGITSDKLLLAW
0 4 S550 DIVDLQRSPMGRkQG
1 4 K555‑ub QRSPMGRkQGGTLDD
0 1 T559 MGRkQGGTLDDLEEQ
0 1 T583 EKPRDQRTEGDSQEM
0 1 S587 DQRTEGDSQEMVRLL
0 2 S600 LLLQAIQSFEKKVRV
0 1 Y609 EKKVRVIYTQLSKTV
0 1 T610 KKVRVIYTQLSKTVV
0 1 S634 PKVEEVVSLMNEDER
0 1 K659 KELWNLLKIACSKVR
0 11 S670 SKVRGPVSGsPDsMN
0 75 S672‑p VRGPVSGsPDsMNVs
0 8 S675‑p PVSGsPDsMNVsRLs
1 5 S679‑p sPDsMNVsRLsHPGQ
1 1 S682‑p sMNVsRLsHPGQLMs
1 0 S689‑p sHPGQLMsQPsSACD
1 0 S692‑p GQLMsQPsSACDsLP
0 1 S693 QLMsQPsSACDsLPE
0 1 C695 MsQPsSACDsLPEsD
1 3 S697‑p QPsSACDsLPEsDKK
1 0 S701‑p ACDsLPEsDKKsEEL
1 0 K703 DsLPEsDKKsEELVA
1 0 S705‑p LPEsDKKsEELVAEA
1 1 S733 TVKEQDRSFTTLDWS
1 0 S733 TVKEQDRSFTTLDWS
0 1 T735 KEQDRSFTTLDWSWL
1 1 S740 SFTTLDWSWLQMEDE
2 0 C750 QMEDEERCSLEQACD
2078 : Phospho-IKKalpha (Ser176)/IKKbeta (Ser177) (C84E11) Rabbit mAb
2694 : Phospho-IKKalpha/beta (Ser176/180) Antibody II
2697 : Phospho-IKK-alpha/beta (Ser176/180) (16A6) Rabbit mAb
14938 : Phospho-IKK alpha/beta (Ser176/180) (16A6) Rabbit mAb (PE Conjugate)
2694 : Phospho-IKKalpha/beta (Ser176/180) Antibody II
2697 : Phospho-IKK-alpha/beta (Ser176/180) (16A6) Rabbit mAb
14938 : Phospho-IKK alpha/beta (Ser176/180) (16A6) Rabbit mAb (PE Conjugate)
  human

 
S4‑p ____MSWsPsLTTQT
S6‑p __MSWsPsLTTQTCG
T23‑p EMKERLGtGGFGNVI
K106‑ub CQGGDLRkYLNQFEN
K147‑ub RIIHRDLkPENIVLQ
K163‑ub GEQRLIHkIIDLGYA
K171‑ub IIDLGYAkELDQGsL
S177‑p AkELDQGsLCtsFVG
T180‑p LDQGsLCtsFVGTLQ
T180‑ac LDQGsLCtsFVGTLQ
S181‑p DQGsLCtsFVGTLQy
Y188‑p sFVGTLQyLAPELLE
Y199‑p ELLEQQKyTVTVDYW
S239‑p HSKVRQKsEVDIVVs
S246‑p sEVDIVVsEDLNGTV
S256‑p LNGTVKFsssLPyPN
S257‑p NGTVKFsssLPyPNN
S258‑p GTVKFsssLPyPNNL
Y261‑p KFsssLPyPNNLNsV
S267‑p PyPNNLNsVLAERLE
T324‑p MVTGTIHtyPVTEDE
Y325‑p VTGTIHtyPVTEDEs
S332‑p yPVTEDEsLQsLKAR
S335‑p TEDEsLQsLKARIQQ
T344‑p KARIQQDtGIPEEDQ
T368‑p LIPDKPAtQCISDGK
S393‑p LVFLFDNsKItyEtQ
T396‑p LFDNsKItyEtQIsP
Y397‑p FDNsKItyEtQIsPR
T399‑p NsKItyEtQIsPRPQ
S402‑p ItyEtQIsPRPQPEs
S409‑p sPRPQPEsVsCILQE
S411‑p RPQPEsVsCILQEPk
K418‑ub sCILQEPkRNLAFFQ
S466 LRNNSCLSKMKNsMA
S471‑p CLSKMKNsMAsMsQQ
S474‑p KMKNsMAsMsQQLKA
S476‑p KNsMAsMsQQLKAKL
T488‑p AKLDFFKtsIQIDLE
S489‑p KLDFFKtsIQIDLEK
S498 QIDLEKYSEQTEFGI
S507‑p QTEFGITsDKLLLAW
S550‑p DIVDLQRsPMGRkQG
K555‑ub QRsPMGRkQGGtLDD
T559‑p MGRkQGGtLDDLEEQ
T583‑p EKPRDQRtEGDsQEM
S587‑p DQRtEGDsQEMVRLL
S600‑p LLLQAIQsFEKKVRV
Y609‑p EKKVRVIytQLSKTV
T610‑p KKVRVIytQLSKTVV
S634‑p PKVEEVVsLMNEDEK
K659‑ac KELWNLLkIACSKVR
S670‑p SKVRGPVsGsPDsMN
S672‑p VRGPVsGsPDsMNAs
S675‑p PVsGsPDsMNAsRLs
S679‑p sPDsMNAsRLsQPGQ
S682‑p sMNAsRLsQPGQLMS
S689 sQPGQLMSQPStAsN
S692 GQLMSQPStAsNsLP
T693‑p QLMSQPStAsNsLPE
S695‑p MSQPStAsNsLPEPA
S697‑p QPStAsNsLPEPAkK
P701 AsNsLPEPAkKSEEL
K703‑ub NsLPEPAkKSEELVA
S705 LPEPAkKSEELVAEA
S733‑p TVREQDQsFtALDWs
S733‑gl TVREQDQsFtALDWs
T735‑p REQDQsFtALDWsWL
S740‑p sFtALDWsWLQTEEE
S750‑p QTEEEEHsCLEQAS_
2078 : Phospho-IKKalpha (Ser176)/IKKbeta (Ser177) (C84E11) Rabbit mAb
2694 : Phospho-IKKalpha/beta (Ser176/180) Antibody II
2697 : Phospho-IKK-alpha/beta (Ser176/180) (16A6) Rabbit mAb
14938 : Phospho-IKK alpha/beta (Ser176/180) (16A6) Rabbit mAb (PE Conjugate)
2694 : Phospho-IKKalpha/beta (Ser176/180) Antibody II
2697 : Phospho-IKK-alpha/beta (Ser176/180) (16A6) Rabbit mAb
14938 : Phospho-IKK alpha/beta (Ser176/180) (16A6) Rabbit mAb (PE Conjugate)
  rat

 
S4 ____MSWSPSLPTQT
S6 __MSWSPSLPTQTCG
T23 EMKERLGTGGFGNVI
R106 CQGGDLRRYLNQFEN
K147 RIIHRDLKPENIVLQ
K163 GEKRLIHKIIDLGYA
K171 IIDLGYAKELDQGsL
S177‑p AKELDQGsLCTsFVG
T180 LDQGsLCTsFVGTLQ
T180 LDQGsLCTsFVGTLQ
S181‑p DQGsLCTsFVGTLQY
Y188 sFVGTLQYLAPELLE
Y199 ELLEQQKYTVTVDYW
S239 HSKVRQKSEVDIVVS
S246 SEVDIVVSEDLNGTV
S256 LNGTVKFSSSSPFPN
S257 NGTVKFSSSSPFPNN
S258 GTVKFSSSSPFPNNL
F261 KFSSSSPFPNNLNSV
S267 PFPNNLNSVLAERLE
T324 MVTGTIHTYPVMEDE
Y325 VTGTIHTYPVMEDES
S332 YPVMEDESLQSLKTR
S335 MEDESLQSLKTRIRE
T344 KTRIREDTGILETDQ
T368 LLPDKPATQCISDSK
S393 LVFLFDNSKMSYETQ
S396 LFDNSKMSYETQITP
Y397 FDNSKMSYETQITPR
T399 NSKMSYETQITPRPQ
T402 MSYETQITPRPQPES
S409 TPRPQPESVSCVLQE
S411 RPQPESVSCVLQEPK
K418 SCVLQEPKRNLSFFQ
S466 LRNNSCLSKMKNAMA
A471 CLSKMKNAMASTAQQ
S474 KMKNAMASTAQQLKA
A476 KNAMASTAQQLKAKL
T488 AKLDFFKTSIQIDLE
S489 KLDFFKTSIQIDLEK
R498 QIDLEKYREQTEFGI
S507 QTEFGITSDKLLLAW
S550 DIVDLQRSPMGRKQG
K555 QRSPMGRKQGGTLDD
T559 MGRKQGGTLDDLEEQ
T583 EKPRDQRTEGDSQDM
S587 DQRTEGDSQDMVRLL
S600 LLLQAIQSFEKKVRV
Y609 EKKVRVIYSQLSKTV
S610 KKVRVIYSQLSKTVV
R634 PKVEEVVRLMNEDEK
K659 KELWNLLKIACSKVR
S670 SKVRGPVSGsPDSMN
S672‑p VRGPVSGsPDSMNVS
S675 PVSGsPDSMNVSRLS
S679 sPDSMNVSRLSHPGH
S682 SMNVSRLSHPGHLMS
S689 SHPGHLMSQPSSACD
S692 GHLMSQPSSACDSLP
S693 HLMSQPSSACDSLPD
C695 MSQPSSACDSLPDSD
S697 QPSSACDSLPDSDKK
S701 ACDSLPDSDKKSEEL
K703 DSLPDSDKKSEELVA
S705 LPDSDKKSEELVAEA
S733 TVKQQDRSFTTLDWS
S733 TVKQQDRSFTTLDWS
T735 KQQDRSFTTLDWSWL
S740 SFTTLDWSWLQMEDE
C750 QMEDEERCGLEQACD
2078 : Phospho-IKKalpha (Ser176)/IKKbeta (Ser177) (C84E11) Rabbit mAb
2694 : Phospho-IKKalpha/beta (Ser176/180) Antibody II
2697 : Phospho-IKK-alpha/beta (Ser176/180) (16A6) Rabbit mAb
14938 : Phospho-IKK alpha/beta (Ser176/180) (16A6) Rabbit mAb (PE Conjugate)
2694 : Phospho-IKKalpha/beta (Ser176/180) Antibody II
2697 : Phospho-IKK-alpha/beta (Ser176/180) (16A6) Rabbit mAb
14938 : Phospho-IKK alpha/beta (Ser176/180) (16A6) Rabbit mAb (PE Conjugate)
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