Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage Cell Signaling Technology
PhosphoSitePlus
HomeAbout PhosphoSiteUsing PhosphoSiteprivacy & cookiesCuration ProcessContact
logos LINCs Logo Mt Sinai Logo NIH Logo NCI Logo
Protein Page:
CTNNB1 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitylation
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
CTNNB1 a regulator of cell adhesion and a key downstream effector in the Wnt signaling pathway. Implicated early embryonic development and tumorigenesis. Phosphorylated and destabilized by CK1 and GSK-3beta. Stabilized cytoplasmic beta-catenin is a hallmark of a variety of cancers. Stabilized beta-catenin translocates to the nucleus, where it acts as a transcriptional activator of T-cell factor (TCF)-regulated genes. Interacts with the PDZ domain of TAX1BP3, inhibiting its transcriptional activity. Two alternatively spliced human isoforms have been described. Note: This description may include information from UniProtKB.
Protein type: Nuclear receptor co-regulator; Motility/polarity/chemotaxis; Actin-binding; Cell adhesion; Transcription factor; Oncoprotein
Chromosomal Location of Human Ortholog: 3p21
Cellular Component: adherens junction; basolateral plasma membrane; beta-catenin destruction complex; catenin complex; cell cortex; cell junction; cell-cell adherens junction; centrosome; cytoplasm; cytosol; focal adhesion; intercellular junction; lateral plasma membrane; membrane; nucleoplasm; nucleus; perinuclear region of cytoplasm; plasma membrane; protein complex; transcription factor complex
Molecular Function: alpha-catenin binding; cadherin binding; enzyme binding; estrogen receptor binding; kinase binding; nuclear hormone receptor binding; protein binding; protein C-terminus binding; protein phosphatase binding; SMAD binding; transcription coactivator activity; transcription factor binding
Biological Process: cell adhesion; cell-cell adhesion; epithelial to mesenchymal transition; hair cell differentiation; negative regulation of cell proliferation; negative regulation of mitotic cell cycle, embryonic; negative regulation of protein sumoylation; negative regulation of transcription, DNA-dependent; patterning of blood vessels; positive regulation of apoptosis; positive regulation of histone H3-K4 methylation; positive regulation of interferon type I production; positive regulation of muscle cell differentiation; positive regulation of neuroblast proliferation; positive regulation of neuron apoptosis; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; proteasomal ubiquitin-dependent protein catabolic process; regulation of angiogenesis; regulation of centriole-centriole cohesion; regulation of fibroblast proliferation; regulation of neurogenesis; regulation of smooth muscle cell proliferation; response to drug; response to estradiol stimulus; stem cell maintenance; Wnt receptor signaling pathway; Wnt receptor signaling pathway through beta-catenin; Wnt receptor signaling pathway, calcium modulating pathway
Disease: Colorectal Cancer; Hepatocellular Carcinoma; Mental Retardation, Autosomal Dominant 19; Ovarian Cancer; Pilomatrixoma
Reference #:  P35222 (UniProtKB)
Alt. Names/Synonyms: Beta-catenin; catenin (cadherin-associated protein), beta 1, 88kDa; Catenin beta-1; CTNB1; CTNNB; CTNNB1; DKFZp686D02253; FLJ25606; FLJ37923
Gene Symbols: CTNNB1
Molecular weight: 85,497 Da
Basal Isoelectric point: 5.53  Predict pI for various phosphorylation states
CST Pathways:  Adherens Junction Dynamics  |  ErbB/HER Signaling  |  ESC Pluripotency and Differentiation  |  Wnt/ß-Catenin Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

CTNNB1

Protein Structure Not Found.
Download PyMol Script
Download ChimeraX Script


STRING  |  cBioPortal  |  Wikipedia  |  Reactome  |  neXtProt  |  Protein Atlas  |  BioGPS  |  Scansite  |  Pfam  |  RCSB PDB  |  Phospho3D  |  Phospho.ELM  |  NetworKIN  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene  |  NURSA  |  Ensembl Protein


Sites Implicated In
apoptosis, induced: T332‑p, T472‑p, S552‑p
carcinogenesis, induced: Y333‑p
cell adhesion, altered: S33‑p, S37‑p, T41‑p, S45‑p
cell cycle regulation: Y333‑p
cell growth, altered: S33‑p, S37‑p, T41‑p, Y86‑p, Y654‑p, Y670‑p
cell growth, induced: Y333‑p, S605‑p, Y654‑p, Y670‑p
cell growth, inhibited: S45‑p, T112‑p, T120‑p
cell motility, altered: S29‑p, S45‑p, T102‑p, T112‑p, S552‑p
cell motility, inhibited: T112‑p, T120‑p
cytoskeletal reorganization: S33‑p, S37‑p, T41‑p, Y654‑p
transcription, altered: S33‑p, S37‑p, T41‑p, Y86‑p, T393‑p, Y654‑p
transcription, induced: Y86‑p, T112‑p, T120‑p, S191‑p, S246‑p, Y333‑p, T393‑p, Y489‑p, S552‑p, S605‑p, Y654‑p, Y670‑p, S675‑p
transcription, inhibited: S33‑p, S37‑p, S45‑p, T120‑p, T332‑p, T472‑p, S552‑p
activity, induced: T393‑p, Y654‑p, Y670‑p, S675‑p
activity, inhibited: S33‑p
intracellular localization: S33‑p, S37‑p, T41‑p, S45‑p, T112‑p, T120‑p, Y142‑p, S191‑p, S246‑p, Y333‑p, Y489‑p, S552‑p, S605‑p, S646‑p, Y654‑p, Y670‑p, S675‑p
molecular association, regulation: S33‑p, S37‑p, T41‑p, S45‑p, Y86‑p, Y142‑p, S191‑p, S246‑p, Y333‑p, T393‑p, Y489‑p, S605‑p, Y654‑p, Y670‑p, S675‑p, S715‑p
phosphorylation: S45‑p, T393‑p
protein conformation: S191‑p, S246‑p, S605‑p
protein degradation: S29‑p, S33‑p, S37‑p, T41‑p, S45‑p, T102‑p, T112‑p, S715‑p
protein stabilization: Y86‑p, S191‑p, S246‑p, T393‑p, S605‑p, Y654‑p, S675‑p
ubiquitination: S33‑p, S37‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment



 LTP 

LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 HTP 

HTP: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
1 0 K19‑ac MAMEPDRkAAVsHWQ
1 3 K19‑ub MAMEPDRkAAVsHWQ
2 0 S23‑p PDRkAAVsHWQQQsy
0 1 S23 PDRkAAVSHWQQQsy
3 4 S29‑p VsHWQQQsyLDsGIH
0 38 Y30‑p sHWQQQsyLDsGIHs
64 5 S33‑p QQQsyLDsGIHsGAT
66 14 S37‑p yLDsGIHsGATtTAP
56 2 T41‑p GIHsGATtTAPsLsG
27 5 S45‑p GATtTAPsLsGkGNP
0 1 S47‑p TtTAPsLsGkGNPEE
6 0 K49‑ac TAPsLsGkGNPEEED
1 0 K49‑ub TAPsLsGkGNPEEED
1 0 Y64‑p VDTSQVLyEWEQGFS
1 0 S73‑p WEQGFSQsFTQEQVA
6 23 Y86‑p VADIDGQyAMTRAQR
2 0 T102‑p RAAMFPEtLDEGMQI
2 0 T112‑p EGMQIPStQFdAAHP
0 1 D115‑ca QIPStQFdAAHPtNV
3 0 T120‑p QFdAAHPtNVQRLAE
0 7 K133‑ub AEPSQMLkHAVVNLI
11 1 Y142‑p AVVNLINyQDDAELA
0 3 K158‑ub RAIPELTkLLNDEDQ
0 9 K170‑ub EDQVVVNkAAVMVHQ
0 3 S179‑p AVMVHQLskKEASRH
0 6 K180‑ub VMVHQLskKEASRHA
4 47 S191‑p SRHAIMRsPQMVsAI
0 2 S196‑p MRsPQMVsAIVRTMQ
0 2 S222‑p AGTLHNLsHHREGLL
0 13 K233‑ub EGLLAIFkSGGIPAL
3 0 S246‑p ALVKMLGsPVDSVLF
1 1 Y331‑p LVNIMRTytyEkLLW
1 0 T332‑p VNIMRTytyEkLLWT
2 1 Y333‑p NIMRTytyEkLLWTT
0 3 K335‑ub MRTytyEkLLWTTSR
2 0 K345‑ac WTTSRVLkVLSVCSS
0 1 K345‑ub WTTSRVLkVLSVCSS
1 0 K354‑ac LSVCSSNkPAIVEAG
0 6 K354‑ub LSVCSSNkPAIVEAG
2 0 T393‑p RNLSDAAtkQEGMEG
1 0 K394‑ub NLSDAAtkQEGMEGL
0 1 K435‑ac TCNNYKNkMMVCQVG
0 6 K435‑ub TCNNYKNkMMVCQVG
0 1 T461‑p AGDREDItEPAICAL
1 1 T472‑p ICALRHLtsRHQEAE
0 1 S473‑p CALRHLtsRHQEAEM
1 90 Y489‑p QNAVRLHyGLPVVVK
0 31 K508‑ub PSHWPLIkAtVGLIR
0 1 T510‑p HWPLIkAtVGLIRNL
0 1 T547‑p LVRAHQDtQRRtsMG
0 16 T551‑p HQDtQRRtsMGGtQQ
15 55 S552‑p QDtQRRtsMGGtQQQ
0 22 T556‑p RRtsMGGtQQQFVEG
0 2 T574‑p EEIVEGCtGALHILA
3 0 S605‑p LFVQLLYsPIENIQR
0 6 K625‑ub LCELAQDkEAAEAIE
1 0 S646‑p PLTELLHsRNEGVAt
0 1 T653‑p sRNEGVAtyAAAVLF
16 21 Y654‑p RNEGVAtyAAAVLFR
2 15 Y670‑p SEDKPQDykKRLsVE
0 4 K671‑ub EDKPQDykKRLsVEL
16 52 S675‑p QDykKRLsVELtsSL
0 3 T679‑p KRLsVELtsSLFRTE
0 1 S680‑p RLsVELtsSLFRTEP
0 1 S681 LsVELtsSLFRTEPM
0 1 T685 LtsSLFRTEPMAWNE
0 1 T693 EPMAWNETADLGLDI
1 1 S715‑p GYRQDDPsyRsFHsG
0 36 Y716‑p YRQDDPsyRsFHsGG
1 4 S718‑p QDDPsyRsFHsGGyG
0 2 S721‑p PsyRsFHsGGyGQDA
0 1 Y724‑p RsFHsGGyGQDALGM
0 1 Y748‑p GHHPGADyPVDGLPD
2009 : Phospho-beta-Catenin (Ser33/37) Antibody
4270 : Non-phospho-beta-Catenin (Ser33/37/Thr41) Antibody
9561 : Phospho-beta-Catenin (Ser33/37/Thr41) Antibody
13537 : Non-phospho (Active) beta-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb (PE Conjugate)
2009 : Phospho-beta-Catenin (Ser33/37) Antibody
4270 : Non-phospho-beta-Catenin (Ser33/37/Thr41) Antibody
9561 : Phospho-beta-Catenin (Ser33/37/Thr41) Antibody
13537 : Non-phospho (Active) beta-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb (PE Conjugate)
4270 : Non-phospho-beta-Catenin (Ser33/37/Thr41) Antibody
9561 : Phospho-beta-Catenin (Ser33/37/Thr41) Antibody
9565 : Phospho-beta-Catenin (Thr41/Ser45) Antibody
13537 : Non-phospho (Active) beta-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb (PE Conjugate)
9564 : Phospho-beta-Catenin (Ser45) Antibody
9565 : Phospho-beta-Catenin (Thr41/Ser45) Antibody
19807 : Non-phospho (Active) beta-Catenin (Ser45) (D2U8Y) XP(R) Rabbit mAb
9030 : Acetyl-beta-Catenin (Lys49) (D7C2) Rabbit mAb
9534 : Acetyl-beta-Catenin (Lys49) Antibody
5651 : Phospho-beta-Catenin (Ser552) (D8E11) Rabbit mAb
9566 : Phospho-beta-Catenin (Ser552) Antibody
4176 : Phospho-beta-Catenin (Ser675) (D2F1) XP(TM) Rabbit mAb
9567 : Phospho-beta-Catenin (Ser675) Antibody
  mouse

 
K19 MAMEPDRKAAVsHWQ
K19‑ub MAMEPDRkAAVsHWQ
S23‑p PDRkAAVsHWQQQsY
S23‑gl PDRkAAVsHWQQQsY
S29‑p VsHWQQQsYLDsGIH
Y30 sHWQQQsYLDsGIHs
S33‑p QQQsYLDsGIHsGAT
S37‑p YLDsGIHsGATtTAP
T41‑p GIHsGATtTAPsLSG
S45‑p GATtTAPsLSGkGNP
S47 TtTAPsLSGkGNPEE
K49‑ac TAPsLSGkGNPEEED
K49 TAPsLSGKGNPEEED
Y64 VDTSQVLYEWEQGFS
S73 WEQGFSQSFTQEQVA
Y86‑p VADIDGQyAMTRAQR
T102 RAAMFPETLDEGMQI
T112 EGMQIPSTQFDAAHP
D115 QIPSTQFDAAHPTNV
T120 QFDAAHPTNVQRLAE
K133‑ub AEPSQMLkHAVVNLI
Y142‑p AVVNLINyQDDAELA
K158‑ub RAIPELTkLLNDEDQ
K170‑ub EDQVVVNkAAVMVHQ
S179‑p AVMVHQLsKKEASRH
K180 VMVHQLsKKEASRHA
S191‑p SRHAIMRsPQMVSAI
S196 MRsPQMVSAIVRTMQ
S222‑p AGTLHNLsHHREGLL
K233‑ub EGLLAIFkSGGIPAL
S246 ALVKMLGSPVDSVLF
Y331 LVNIMRTYTYEKLLW
T332 VNIMRTYTYEKLLWT
Y333 NIMRTYTYEKLLWTT
K335 MRTYTYEKLLWTTSR
K345 WTTSRVLKVLSVCSS
K345 WTTSRVLKVLSVCSS
K354 LSVCSSNKPAIVEAG
K354 LSVCSSNKPAIVEAG
T393‑p RNLSDAAtKQEGMEG
K394 NLSDAAtKQEGMEGL
K435 TCNNYKNKMMVCQVG
K435 TCNNYKNKMMVCQVG
T461‑p AGDREDItEPAICAL
T472 ICALRHLTSRHQEAE
S473 CALRHLTSRHQEAEM
Y489‑p QNAVRLHyGLPVVVK
K508 PSHWPLIKATVGLIR
T510 HWPLIKATVGLIRNL
T547 LVRAHQDTQRRtsMG
T551‑p HQDTQRRtsMGGtQQ
S552‑p QDTQRRtsMGGtQQQ
T556‑p RRtsMGGtQQQFVEG
T574 EEIVEGCTGALHILA
S605‑p LFVQLLYsPIENIQR
K625‑ub LCELAQDkEAAEAIE
S646 PLTELLHSRNEGVAT
T653 SRNEGVATyAAAVLF
Y654‑p RNEGVATyAAAVLFR
Y670 SEDKPQDYkKRLsVE
K671‑ub EDKPQDYkKRLsVEL
S675‑p QDYkKRLsVELtSsL
T679‑p KRLsVELtSsLFRtE
S680 RLsVELtSsLFRtEP
S681‑p LsVELtSsLFRtEPM
T685‑p LtSsLFRtEPMAWNE
T693‑p EPMAWNEtADLGLDI
S715 GYRQDDPSYRSFHSG
Y716 YRQDDPSYRSFHSGG
S718 QDDPSYRSFHSGGYG
S721 PSYRSFHSGGYGQDA
Y724 RSFHSGGYGQDALGM
Y748 GHHPGADYPVDGLPD
2009 : Phospho-beta-Catenin (Ser33/37) Antibody
4270 : Non-phospho-beta-Catenin (Ser33/37/Thr41) Antibody
9561 : Phospho-beta-Catenin (Ser33/37/Thr41) Antibody
13537 : Non-phospho (Active) beta-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb (PE Conjugate)
2009 : Phospho-beta-Catenin (Ser33/37) Antibody
4270 : Non-phospho-beta-Catenin (Ser33/37/Thr41) Antibody
9561 : Phospho-beta-Catenin (Ser33/37/Thr41) Antibody
13537 : Non-phospho (Active) beta-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb (PE Conjugate)
4270 : Non-phospho-beta-Catenin (Ser33/37/Thr41) Antibody
9561 : Phospho-beta-Catenin (Ser33/37/Thr41) Antibody
9565 : Phospho-beta-Catenin (Thr41/Ser45) Antibody
13537 : Non-phospho (Active) beta-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb (PE Conjugate)
9564 : Phospho-beta-Catenin (Ser45) Antibody
9565 : Phospho-beta-Catenin (Thr41/Ser45) Antibody
19807 : Non-phospho (Active) beta-Catenin (Ser45) (D2U8Y) XP(R) Rabbit mAb
9030 : Acetyl-beta-Catenin (Lys49) (D7C2) Rabbit mAb
9534 : Acetyl-beta-Catenin (Lys49) Antibody
5651 : Phospho-beta-Catenin (Ser552) (D8E11) Rabbit mAb
9566 : Phospho-beta-Catenin (Ser552) Antibody
4176 : Phospho-beta-Catenin (Ser675) (D2F1) XP(TM) Rabbit mAb
9567 : Phospho-beta-Catenin (Ser675) Antibody
  rat

 
K19 MAMEPDRKAAVSHWQ
K19 MAMEPDRKAAVSHWQ
S23 PDRKAAVSHWQQQSY
S23 PDRKAAVSHWQQQSY
S29 VSHWQQQSYLDsGIH
Y30 SHWQQQSYLDsGIHs
S33‑p QQQSYLDsGIHsGAT
S37‑p YLDsGIHsGATtTAP
T41‑p GIHsGATtTAPsLSG
S45‑p GATtTAPsLSGKGNP
S47 TtTAPsLSGKGNPEE
K49 TAPsLSGKGNPEEED
K49 TAPsLSGKGNPEEED
Y64 VDTSQVLYEWEQGFS
S73 WEQGFSQSFTQEQVA
Y86 VADIDGQYAMTRAQR
T102 RAAMFPETLDEGMQI
T112 EGMQIPSTQFDAAHP
D115 QIPSTQFDAAHPTNV
T120 QFDAAHPTNVQRLAE
K133 AEPSQMLKHAVVNLI
Y142‑p AVVNLINyQDDAELA
K158 RAIPELTKLLNDEDQ
K170 EDQVVVNKAAVMVHQ
S179 AVMVHQLSKKEASRH
K180 VMVHQLSKKEASRHA
S191‑p SRHAIMRsPQMVSAI
S196 MRsPQMVSAIVRTMQ
S222 AGTLHNLSHHREGLL
K233 EGLLAIFKSGGIPAL
S246 ALVKMLGSPVDSVLF
Y331 LVNIMRTYTYEKLLW
T332 VNIMRTYTYEKLLWT
Y333 NIMRTYTYEKLLWTT
K335 MRTYTYEKLLWTTSR
K345 WTTSRVLKVLSVCSS
K345 WTTSRVLKVLSVCSS
K354 LSVCSSNKPAIVEAG
K354 LSVCSSNKPAIVEAG
T393 RNLSDAATKQEGMEG
K394 NLSDAATKQEGMEGL
K435 TCNNYKNKMMVCQVG
K435 TCNNYKNKMMVCQVG
T461 AGDREDITEPAICAL
T472 ICALRHLTSRHQEAE
S473 CALRHLTSRHQEAEM
Y489‑p QNAVRLHyGLPVVVK
K508 PSHWPLIKATVGLIR
T510 HWPLIKATVGLIRNL
T547 LVRAHQDTQRRtsMG
T551‑p HQDTQRRtsMGGTQQ
S552‑p QDTQRRtsMGGTQQQ
T556 RRtsMGGTQQQFVEG
T574 EEIVEGCTGALHILA
S605 LFVQLLYSPIENIQR
K625 LCELAQDKEAAEAIE
S646 PLTELLHSRNEGVAT
T653 SRNEGVATyAAAVLF
Y654‑p RNEGVATyAAAVLFR
Y670 SEDKPQDYKKRLsVE
K671 EDKPQDYKKRLsVEL
S675‑p QDYKKRLsVELTSSL
T679 KRLsVELTSSLFRTE
S680 RLsVELTSSLFRTEP
S681 LsVELTSSLFRTEPM
T685 LTSSLFRTEPMAWNE
T693 EPMAWNETADLGLDI
S715 GYRQDDPSYRSFHSG
Y716 YRQDDPSYRSFHSGG
S718 QDDPSYRSFHSGGYG
S721 PSYRSFHSGGYGQDA
Y724 RSFHSGGYGQDALGM
Y748 GHHPGADYPVDGLPD
2009 : Phospho-beta-Catenin (Ser33/37) Antibody
4270 : Non-phospho-beta-Catenin (Ser33/37/Thr41) Antibody
9561 : Phospho-beta-Catenin (Ser33/37/Thr41) Antibody
13537 : Non-phospho (Active) beta-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb (PE Conjugate)
2009 : Phospho-beta-Catenin (Ser33/37) Antibody
4270 : Non-phospho-beta-Catenin (Ser33/37/Thr41) Antibody
9561 : Phospho-beta-Catenin (Ser33/37/Thr41) Antibody
13537 : Non-phospho (Active) beta-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb (PE Conjugate)
4270 : Non-phospho-beta-Catenin (Ser33/37/Thr41) Antibody
9561 : Phospho-beta-Catenin (Ser33/37/Thr41) Antibody
9565 : Phospho-beta-Catenin (Thr41/Ser45) Antibody
13537 : Non-phospho (Active) beta-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb (PE Conjugate)
9565 : Phospho-beta-Catenin (Thr41/Ser45) Antibody
19807 : Non-phospho (Active) beta-Catenin (Ser45) (D2U8Y) XP(R) Rabbit mAb
9030 : Acetyl-beta-Catenin (Lys49) (D7C2) Rabbit mAb
9534 : Acetyl-beta-Catenin (Lys49) Antibody
5651 : Phospho-beta-Catenin (Ser552) (D8E11) Rabbit mAb
9566 : Phospho-beta-Catenin (Ser552) Antibody
4176 : Phospho-beta-Catenin (Ser675) (D2F1) XP(TM) Rabbit mAb
9567 : Phospho-beta-Catenin (Ser675) Antibody
Home  |  Curator Login With enhanced literature mining using Linguamatics I2E I2E Logo Produced by 3rd Millennium  |  Design by Digizyme
©2003-2013 Cell Signaling Technology, Inc.