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Protein Page:
Btk (mouse)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitylation
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
Btk a tyrosine kinase of the Tec family. Plays a crucial role in B-cell ontogeny. Defects cause X-linked agammaglobulinemia, an immunodeficiency characterized by failure to produce mature B lymphocyte cells and associated with a failure of Ig heavy chain rearrangement. Truncated splice forms found in childhood leukemias may underlie radiation resistance of tumors through inhibition of apoptosis. Note: This description may include information from UniProtKB.
Protein type: EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); Kinase, protein; Protein kinase, TK; TK group; Tec family
Cellular Component: cytoplasm; cytoplasmic vesicle; cytosol; extrinsic to internal side of plasma membrane; intracellular membrane-bound organelle; lipid raft; mast cell granule; membrane; nucleus; perinuclear region of cytoplasm; plasma membrane
Molecular Function: ATP binding; identical protein binding; kinase activity; lipid binding; metal ion binding; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; phosphatidylinositol-3,4,5-triphosphate binding; protein binding; protein kinase activity; protein-tyrosine kinase activity; transferase activity
Biological Process: adaptive immune response; apoptosis; cell maturation; histamine secretion by mast cell; I-kappaB kinase/NF-kappaB cascade; immune system process; innate immune response; negative regulation of cytokine production; peptidyl-tyrosine phosphorylation; phosphorylation; positive regulation of type I hypersensitivity; positive regulation of type III hypersensitivity; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of cell proliferation; regulation of transcription, DNA-dependent; response to organic substance; transcription, DNA-dependent; transmembrane receptor protein tyrosine kinase signaling pathway
Reference #:  P35991 (UniProtKB)
Gene Symbols: Btk
Molecular weight: 76,437 Da
Basal Isoelectric point: 8.05  Predict pI for various phosphorylation states
CST Pathways:  B Cell Receptor Signaling  |  Tyrosine Kinases & Substrates
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
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Btk

Protein Structure Not Found.
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Substrate Sequence Logo
Sequence Logo

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Sites Implicated In
cell growth, altered: Y223‑p, Y551‑p
activity, inhibited: S180‑p
enzymatic activity, induced: Y223‑p, Y551‑p
intracellular localization: S180‑p
molecular association, regulation: S21‑p, S115‑p, Y223‑p
not reported: Y375‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 LTP 

LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 HTP 

HTP: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       mouse

 
1 3 S21‑p SQQKKKTsPLNFKKR
0 26 Y40‑p TVHKLSYyEYDFERG
1 0 S51 FERGRRGSKKGSIDV
0 2 S55 RRGSKKGSIDVEKIT
0 1 S93 SSEMEQISIIERFPY
1 0 S115‑p EGPLYVFsPTEELRK
0 1 S174‑p ILENRNGsLKPGssH
0 2 S179‑p NGsLKPGssHRKTKK
1 3 S180‑p GsLKPGssHRKTKKP
0 1 K183 KPGssHRKTKKPLPP
0 1 T184 PGssHRKTKKPLPPt
0 3 T191‑p TKKPLPPtPEEDQIL
0 1 T207‑p KPLPPEPtAAPISTt
0 1 T214‑p tAAPISTtELKKVVA
18 259 Y223‑p LKKVVALyDyMPMNA
0 90 Y225‑p KVVALyDyMPMNAND
0 1 Y263 DKNGQEGYIPSNYIT
0 1 Y279 AEDSIEMYEWYSKHM
0 1 K300 QLLKQEGKEGGFIVR
0 1 S318 KAGKYTVSVFAKSTG
0 6 K322 YTVSVFAKSTGEPQG
0 7 Y334‑p PQGVIRHyVVCSTPQ
0 1 S342 VVCSTPQSQyyLAEK
0 187 Y344‑p CSTPQSQyyLAEKHL
0 7 Y345‑p STPQSQyyLAEKHLF
0 109 Y361‑p TIPELINyHQHNSAG
0 3 S366 INyHQHNSAGLISRL
0 1 S371 HNSAGLISRLKyPVS
1 25 Y375‑p GLISRLKyPVSKQNK
0 2 S378 SRLKyPVSKQNKNAP
0 13 K406 PKDLTFLKELGTGQF
0 1 Y425‑p YGKWRGQyDVAIKMI
0 1 S436‑p IKMIREGsMSEDEFI
0 15 Y461‑p HEKLVQLyGVCTKQR
0 3 K466 QLyGVCTKQRPIFII
1 0 T495 EMRHRFQTQQLLEMC
0 5 K536 VNDQGVVKVSDFGLs
0 2 S543‑p KVSDFGLsRYVLDDE
23 147 Y551‑p RYVLDDEyTsSVGSK
0 9 T552 YVLDDEyTsSVGSKF
0 4 S553‑p VLDDEyTsSVGSKFP
0 12 K558 yTsSVGSKFPVRWSP
0 1 S564 SKFPVRWSPPEVLMY
0 1 T602 KMPYERFTNSETAEH
0 2 S604 PYERFTNSETAEHIA
1 1 Y617‑p IAQGLRLyRPHLAsE
0 1 S623‑p LyRPHLAsERVYTIM
0 1 S659 LDVMDEES_______
5082 : Phospho-Btk (Tyr223) Antibody
87141 : Phospho-Btk (Tyr223) (D9T6H) Rabbit mAb
  human

 
S21‑p SQQKKKTsPLNFKKR
Y40‑p TVHKLSYyEYDFERG
S51‑p FERGRRGsKKGsIDV
S55‑p RRGsKKGsIDVEKIT
S93‑p SSEMEQIsIIERFPY
S115 EGPLYVFSPTEELRK
S174 ILENRNGSLKPGssH
S179‑p NGSLKPGssHRktKK
S180‑p GSLKPGssHRktKKP
K183‑ac KPGssHRktKKPLPP
T184‑p PGssHRktKKPLPPt
T191‑p tKKPLPPtPEEDQIL
A207 KPLPPEPAAAPVSTS
S214 AAAPVSTSELKKVVA
Y223‑p LKKVVALyDyMPMNA
Y225‑p KVVALyDyMPMNAND
Y263‑p DKNGQEGyIPSNYVT
Y279‑p AEDSIEMyEWYSKHM
K300‑ub QLLKQEGkEGGFIVR
S318‑p KAGKYTVsVFAkSTG
K322‑ub YTVsVFAkSTGDPQG
Y334‑p PQGVIRHyVVCSTPQ
S342‑p VVCSTPQsQyyLAEK
Y344‑p CSTPQsQyyLAEKHL
Y345‑p STPQsQyyLAEKHLF
Y361‑p TIPELINyHQHNsAG
S366‑p INyHQHNsAGLIsRL
S371‑p HNsAGLIsRLKyPVs
Y375‑p GLIsRLKyPVsQQNK
S378‑p sRLKyPVsQQNKNAP
K406‑ub PKDLTFLkELGTGQF
Y425 YGKWRGQYDVAIKMI
S436 IKMIKEGSMSEDEFI
Y461‑p HEKLVQLyGVCTkQR
K466‑ub QLyGVCTkQRPIFII
T495‑p EMRHRFQtQQLLEMC
K536‑ub VNDQGVVkVSDFGLs
S543‑p kVSDFGLsRYVLDDE
Y551‑p RYVLDDEytsSVGSk
T552‑p YVLDDEytsSVGSkF
S553‑p VLDDEytsSVGSkFP
K558‑ub ytsSVGSkFPVRWsP
S564‑p SkFPVRWsPPEVLMY
T602‑p KMPYERFtNsETAEH
S604‑p PYERFtNsETAEHIA
Y617 IAQGLRLYRPHLASE
S623 LYRPHLASEKVYTIM
S659‑p LDVMDEEs_______
3537 : Phospho-Btk (Ser180) (3D3) Mouse mAb
5082 : Phospho-Btk (Tyr223) Antibody
87141 : Phospho-Btk (Tyr223) (D9T6H) Rabbit mAb
  rat

 
S21 SQQKKKTSPLNFKKR
Y40 TVHKLSYYEYDFERG
S51 FERGRRGSKKGSIDV
S55 RRGSKKGSIDVEKIT
S94 SSEMEQISIIERFPY
S116 EGPLYVFSPTEELRK
S175 ILENRNGSLKPGSSH
S180 NGSLKPGSSHRKTKK
S181 GSLKPGSSHRKTKKP
K184 KPGSSHRKTKKPLPP
T185 PGSSHRKTKKPLPPT
T192 TKKPLPPTPEEDQIL
T208 KPLPPEPTAAPISTS
S215 TAAPISTSELKKVVA
Y224 LKKVVALYDYMPMNA
Y226 KVVALYDYMPMNAND
Y264 DKNGQEGYIPSNYVT
Y280 AEDSIEMYEWYSKHM
K301 QLLKQEGKEGGFIVR
S319 KAGKYTVSVFAKSTG
K323 YTVSVFAKSTGEPQG
Y335 PQGVIRHYVVCSTPQ
S343 VVCSTPQSQYYLAEK
Y345 CSTPQSQYYLAEKHL
Y346 STPQSQYYLAEKHLF
Y362 TIPELINYHQHNSAG
S367 INYHQHNSAGLISRL
S372 HNSAGLISRLKYPVS
Y376 GLISRLKYPVSKQNK
S379 SRLKYPVSKQNKNAP
K407 PKDLTFLKELGTGQF
Y426 YGKWRGQYDVAIKMI
S437 IKMIREGSMSEDEFI
Y462‑p HEKLVQLyGVCTKQR
K467 QLyGVCTKQRPIFII
T496 EMRHRFQTQQLLEMC
K537 VNDQGVVKVSDFGLS
S544 KVSDFGLSRYVLDDE
Y552 RYVLDDEYTSSVGSK
T553 YVLDDEYTSSVGSKF
S554 VLDDEYTSSVGSKFP
K559 YTSSVGSKFPVRWSP
S565 SKFPVRWSPPEVLMY
T603 KIPYERFTNSETAEH
S605 PYERFTNSETAEHIA
Y618 IAQGLRLYRPHLASD
S624 LYRPHLASDRVYTIM
S660 LDVMDEES_______
5082 : Phospho-Btk (Tyr223) Antibody
87141 : Phospho-Btk (Tyr223) (D9T6H) Rabbit mAb
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