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Protein Page:
TNIK (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitylation
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
TNIK Serine/threonine kinase that acts as an essential activator of the Wnt signaling pathway. Recruited to promoters of Wnt target genes and required to activate their expression. May act by phosphorylating TCF4/TCF7L2. Appears to act upstream of the JUN N-terminal pathway. May play a role in the response to environmental stress. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. More generally, it may play a role in cytoskeletal rearrangements and regulate cell spreading. Phosphorylates SMAD1 on Thr-322. Interacts (via the CNH domain) with RAP2A (GTP-bound form preferentially); the interaction is direct and required for the activation of TNIK by RAP2A. Interacts with NEDD4; recruits RAP2A to NEDD4. Interacts with TRAF2 and NCK. Interacts with TCF7L2/TCF4 and CTNNB1; the interaction is direct. Interacts with TANC1. Expressed ubiquitously. Highest levels observed in heart, brain and skeletal muscle. Expressed in normal colonic epithelia and colorectal cancer tissues. Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. 8 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Protein kinase, STE; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; STE group; STE20 family; MSN subfamily
Chromosomal Location of Human Ortholog: 3q26.31
Cellular Component: apical plasma membrane; cytoplasm; cytoskeleton; nucleoplasm; nucleus
Molecular Function: protein binding; protein kinase activity; protein serine/threonine kinase activity; receptor signaling protein serine/threonine kinase activity
Biological Process: actin cytoskeleton reorganization; activation of JNKK activity; cytoskeleton organization and biogenesis; microvillus biogenesis; positive regulation of defense response to virus by host; positive regulation of protein amino acid phosphorylation; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of dendrite morphogenesis
Reference #:  Q9UKE5 (UniProtKB)
Alt. Names/Synonyms: KIAA0551; TNIK; TRAF2 and NCK interacting kinase; TRAF2 and NCK-interacting protein kinase; ZC2
Gene Symbols: TNIK
Molecular weight: 154,943 Da
Basal Isoelectric point: 6.7  Predict pI for various phosphorylation states
Select Structure to View Below

TNIK

Protein Structure Not Found.
Download PyMol Script
Download ChimeraX Script


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Sites Implicated In
intracellular localization: S764‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment



 LTP 

LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 HTP 

HTP: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 1 S9 ASDSPARSLDEIDLs
0 1 S16‑p SLDEIDLsALRDPAG
0 3 Y36‑p ELVGNGTyGQVyKGR
0 1 Y40‑p NGTyGQVyKGRHVKT
0 1 K46 VyKGRHVKTGQLAAI
0 8 Y86‑p HRNIATYyGAFIKKN
0 1 K121‑ub TDLIKNTkGNTLKEE
0 5 S175‑p KLVDFGVsAQLDRtV
0 21 T181‑p VsAQLDRtVGRRNtF
1 82 T187‑p RtVGRRNtFIGTPYW
0 1 S264 KWSKKFQSFIESCLV
0 1 T319‑p KRGEKDEtEyEysGs
0 1 Y321‑p GEKDEtEyEysGsEE
0 1 Y323‑p KDEtEyEysGsEEEE
0 4 S324‑p DEtEyEysGsEEEEE
0 5 S326‑p tEyEysGsEEEEEEN
0 1 S335‑p EEEEENDsGEPSSIL
0 3 Y427‑p EREQRRHyEEQMRRE
0 1 Y475‑p EQALLLEyKRKQLEE
0 21 Y499‑p QLKQERDyLVSLQHQ
0 1 S502 QERDyLVSLQHQRQE
0 2 Y519‑p PVEKKPLyHyKEGMs
0 1 Y521‑p EKKPLyHyKEGMsPS
0 3 S526‑p yHyKEGMsPSEKPAW
0 6 S548‑p SRLNRQSsPAMPHKV
0 2 S560‑p HKVANRIsDPNLPPR
0 1 S568 DPNLPPRSEsFSIsG
0 1 S568‑p DPNLPPRsEsFSIsG
0 4 S570‑p NLPPRsEsFSIsGVQ
0 2 S574‑p RsEsFSIsGVQPARt
0 7 T581‑p sGVQPARtPPMLRPV
0 2 S600‑p PHLVAVKsQGPALTA
0 1 T606 KsQGPALTAsQsVHE
0 1 S608‑p QGPALTAsQsVHEQP
0 1 S610‑p PALTAsQsVHEQPTK
0 57 S640‑p VEMPRQNsDPtSENP
0 1 T643‑p PRQNsDPtSENPPLP
0 1 S659 RIEKFDRSSWLRQEE
0 1 T677‑p PKVPQRTtsIsPALA
0 24 S678‑p KVPQRTtsIsPALAR
0 43 S680‑p PQRTtsIsPALARKN
0 14 S688‑p PALARKNsPGNGSAL
0 5 S701‑p ALGPRLGsQPIRAsN
0 37 S707‑p GsQPIRAsNPDLRRT
0 6 S720‑p RTEPILEsPLQRTsS
0 1 T725 LEsPLQRTsSGsSSS
0 1 S726‑p EsPLQRTsSGsSSSS
0 1 S729‑p LQRTsSGsSSSSSTP
0 1 S730 QRTsSGsSSSSSTPS
0 1 S731 RTsSGsSSSSSTPSS
0 1 S732 TsSGsSSSSSTPSSQ
0 1 S755‑p PGSQAGSsERTRVRA
1 42 S764‑p RTRVRANsKsEGsPV
0 9 S766‑p RVRANsKsEGsPVLP
0 50 S769‑p ANsKsEGsPVLPHEP
0 1 S784 AKVKPEESRDITRPS
0 5 S795‑p TRPSRPAsYKKAIDE
0 1 K824‑ac EETNRPMkKVTDYSS
0 1 T902‑p GSISREGtLMIRETS
0 1 S938‑p DLVQQSHsPAGTPTE
0 1 T942 QSHsPAGTPTEGLGR
0 1 S959‑p THSQEMDsGTEyGMG
0 40 Y963‑p EMDsGTEyGMGSSTK
0 1 S972‑p MGSSTKAsFTPFVDP
0 4 Y982‑p PFVDPRVyQTSPtDE
0 1 S985 DPRVyQTSPtDEDEE
0 5 T987‑p RVyQTSPtDEDEEDE
0 1 S996 EDEEDEESSAAALFT
0 1 S997 DEEDEESSAAALFTS
0 1 T1003 SSAAALFTSELLRQE
0 1 S1021‑p LNEARKIsVVNVNPT
0 1 T1036‑p NIRPHSDtPEIRKYK
0 9 S1116‑p KLRVYYLsWLRNRIL
0 1 T1284 LQWGEMPTSVAYIHS
0 1 S1285 QWGEMPTSVAYIHSN
0 2 T1351 SSQVFFMTLNRNSMM
  TNIK iso2  
S9 ASDSPARSLDEIDLS
S16 SLDEIDLSALRDPAG
Y36 ELVGNGTYGQVYKGR
Y40 NGTYGQVYKGRHVKT
K46 VYKGRHVKTGQLAAI
Y86 HRNIATYYGAFIKKN
K121 TDLIKNTKGNTLKEE
S175 KLVDFGVSAQLDRTV
T181 VSAQLDRTVGRRNTF
T187 RTVGRRNTFIGTPYW
S264 KWSKKFQSFIESCLV
T319 KRGEKDETEYEYSGS
Y321 GEKDETEYEYSGSEE
Y323 KDETEYEYSGSEEEE
S324 DETEYEYSGSEEEEE
S326 TEYEYSGSEEEEEEN
S335 EEEEENDSGEPSSIL
Y427 EREQRRHYEEQMRRE
- gap
Y470 QLKQERDYLVSLQHQ
S473 QERDYLVSLQHQRQE
Y490 PVEKKPLYHYKEGMS
Y492 EKKPLYHYKEGMSPS
S497 YHYKEGMSPSEKPAW
S519 SRLNRQSSPAMPHKV
S531 HKVANRISDPNLPPR
S539 DPNLPPRSESFSISG
S539 DPNLPPRSESFSISG
S541 NLPPRSESFSISGVQ
S545 RSESFSISGVQPART
T552 SGVQPARTPPMLRPV
S571 PHLVAVKSQGPALTA
T577 KSQGPALTASQSVHE
S579 QGPALTASQSVHEQP
S581 PALTASQSVHEQPTK
S611 VEMPRQNSDPTSENP
T614 PRQNSDPTSENPPLP
S630 RIEKFDRSSWLRQEE
T648 PKVPQRTTSISPALA
S649 KVPQRTTSISPALAR
S651 PQRTTSISPALARKN
S659 PALARKNSPGNGSAL
S672 ALGPRLGSQPIRASN
S678 GSQPIRASNPDLRRT
S691 RTEPILESPLQRTSS
T696 LESPLQRTSSGSSSS
S697 ESPLQRTSSGSSSSS
S700 LQRTSSGSSSSSSTP
S701 QRTSSGSSSSSSTPS
S702 RTSSGSSSSSSTPSS
S703 TSSGSSSSSSTPSSQ
S726 PGSQAGSSERTRVRA
S735 RTRVRANSKSEGSPV
S737 RVRANSKSEGSPVLP
S740 ANSKSEGSPVLPHEP
S755 AKVKPEESRDITRPS
S766 TRPSRPASYKKAIDE
K795 EETNRPMKKVTDYSS
T873 GSISREGTLMIRETS
S909 DLVQQSHSPAGTPTE
T913 QSHSPAGTPTEGLGR
S930 THSQEMDSGTEYGMG
Y934 EMDSGTEYGMGSSTK
S943 MGSSTKASFTPFVDP
Y953 PFVDPRVYQTSPTDE
S956 DPRVYQTSPTDEDEE
T958 RVYQTSPTDEDEEDE
S967 EDEEDEESSAAALFT
S968 DEEDEESSAAALFTS
T974 SSAAALFTSELLRQE
S992 LNEARKISVVNVNPT
T1007 NIRPHSDTPEIRKYK
S1087 KLRVYYLSWLRNRIL
T1255 LQWGEMPTSVAYIHS
S1256 QWGEMPTSVAYIHSN
T1322 SSQVFFMTLNRNSMM
  TNIK iso3  
S9 ASDSPARSLDEIDLS
S16 SLDEIDLSALRDPAG
Y36 ELVGNGTYGQVYKGR
Y40 NGTYGQVYKGRHVKT
K46 VYKGRHVKTGQLAAI
Y86 HRNIATYYGAFIKKN
K121 TDLIKNTKGNTLKEE
S175 KLVDFGVSAQLDRTV
T181 VSAQLDRTVGRRNTF
T187 RTVGRRNTFIGTPYW
S264 KWSKKFQSFIESCLV
T319 KRGEKDETEYEYSGS
Y321 GEKDETEYEYSGSEE
Y323 KDETEYEYSGSEEEE
S324 DETEYEYSGSEEEEE
S326 TEYEYSGSEEEEEEN
S335 EEEEENDSGEPSSIL
Y427 EREQRRHYEEQMRRE
Y475 EQALLLEYKRKQLEE
Y499 QLKQERDYLVSLQHQ
S502 QERDYLVSLQHQRQE
Y519 PVEKKPLYHYKEGMS
Y521 EKKPLYHYKEGMSPS
S526 YHYKEGMSPSEKPAW
- gap
- gap
- gap
- gap
- gap
- gap
- gap
S545 PHLVAVKSQGPALTA
T551 KSQGPALTASQSVHE
S553 QGPALTASQSVHEQP
S555 PALTASQSVHEQPTK
S585 VEMPRQNSDPTSENP
T588 PRQNSDPTSENPPLP
S604 RIEKFDRSSWLRQEE
T622 PKVPQRTTSISPALA
S623 KVPQRTTSISPALAR
S625 PQRTTSISPALARKN
S633 PALARKNSPGNGSAL
S646 ALGPRLGSQPIRASN
S652 GSQPIRASNPDLRRT
S665 RTEPILESPLQRTSS
T670 LESPLQRTSSGSSSS
S671 ESPLQRTSSGSSSSS
S674 LQRTSSGSSSSSSTP
S675 QRTSSGSSSSSSTPS
S676 RTSSGSSSSSSTPSS
S677 TSSGSSSSSSTPSSQ
S700 PGSQAGSSERTRVRA
S709 RTRVRANSKSEGSPV
S711 RVRANSKSEGSPVLP
S714 ANSKSEGSPVLPHEP
S729 AKVKPEESRDITRPS
S740 TRPSRPASYKKAIDE
K769 EETNRPMKKVTDYSS
T847 GSISREGTLMIRETS
S883 DLVQQSHSPAGTPTE
T887 QSHSPAGTPTEGLGR
S904 THSQEMDSGTEYGMG
Y908 EMDSGTEYGMGSSTK
S917 MGSSTKASFTPFVDP
Y927 PFVDPRVYQTSPTDE
S930 DPRVYQTSPTDEDEE
T932 RVYQTSPTDEDEEDE
S941 EDEEDEESSAAALFT
S942 DEEDEESSAAALFTS
T948 SSAAALFTSELLRQE
S966 LNEARKISVVNVNPT
T981 NIRPHSDTPEIRKYK
S1061 KLRVYYLSWLRNRIL
T1229 LQWGEMPTSVAYIHS
S1230 QWGEMPTSVAYIHSN
T1296 SSQVFFMTLNRNSMM
  mouse

 
S9‑p ASDSPARsLDEIDLS
S16 sLDEIDLSALRDPAG
Y36 ELVGNGTYGQVYKGR
Y40 NGTYGQVYKGRHVKT
K46 VYKGRHVKTGQLAAI
Y86 HRNIATYYGAFIKKN
K121 TDLIKNTKGNTLKEE
S175‑p KLVDFGVsAQLDRtV
T181‑p VsAQLDRtVGRRNtF
T187‑p RtVGRRNtFIGTPYW
S264‑p KWSKKFQsFIESCLV
T319 KRGEKDETEYEYsGs
Y321 GEKDETEYEYsGsEE
Y323 KDETEYEYsGsEEEE
S324‑p DETEYEYsGsEEEEE
S326‑p TEYEYsGsEEEEEEN
S335 EEEEENDSGEPSSIL
Y427 EREQRRHYEEQMRRE
- gap
Y470 QLKQERDYLVsLQHQ
S473‑p QERDYLVsLQHQRQE
Y490 PLEKKPLYHYKEGMs
Y492 EKKPLYHYKEGMsPS
S497‑p YHYKEGMsPSEKPAW
S519‑p SRLNRQSsPAMPHKV
S531 HKVANRISDPNLPPR
S539‑gl DPNLPPRsEsFSIsG
S539 DPNLPPRSEsFSIsG
S541‑p NLPPRsEsFSIsGVQ
S545‑p RsEsFSIsGVQPARt
T552‑p sGVQPARtPPMLRPV
S571 PQLVAVKSQGPALtA
T577‑gl KSQGPALtASQSVHE
S579 QGPALtASQSVHEQP
S581 PALtASQSVHEQPTK
S611‑p VEMPRQNsDPTSENP
T614 PRQNsDPTSENPPLP
S630‑p RIEKFDRsSWLRQEE
T648 PKVPQRTTsIsPALA
S649‑p KVPQRTTsIsPALAR
S651‑p PQRTTsIsPALARKN
S659‑p PALARKNsPGNGSAL
S672‑p ALGPRLGsQPIRAsN
S678‑p GsQPIRAsNPDLRRT
S691 RTEPVLESSLQRTSS
T696 LESSLQRTSSGSSSS
S697 ESSLQRTSSGSSSSS
S700 LQRTSSGSSSSSSTP
S701 QRTSSGSSSSSSTPS
S702 RTSSGSSSSSSTPSS
S703 TSSGSSSSSSTPSSQ
S726 PGSQAGSSERSRVRA
S735‑p RSRVRANsKsEGsPV
S737‑p RVRANsKsEGsPVLP
S740‑p ANsKsEGsPVLPHEP
S755 SKVKPEESRDITRPS
- gap
K787 EETNRPLKKVTDYSS
T865 GSISREGTLMIRETA
S901 DLVQQSHSPAGtPTE
T905‑p QSHSPAGtPTEGLGR
S922 THSQEMDSGAEyGIG
Y926‑p EMDSGAEyGIGSSTK
S935 IGSSTKASFTPFVDP
Y945 PFVDPRVYQTSPtDE
S948 DPRVYQTSPtDEDEE
T950‑p RVYQTSPtDEDEEDD
S959 EDEEDDESSAAALFT
S960 DEEDDESSAAALFTS
T966 SSAAALFTSELLRQE
S984‑p LNEARKIsVVNVNPT
T999 NIRPHSDTPEIRKYK
S1079‑p KLRVYYLsWLRNRIL
T1247‑p LQWGEMPtsVAYIHS
S1248‑p QWGEMPtsVAYIHSN
T1314‑p SSQVFFMtLNRNSMM
  rat

 
S9 ASDSPARSLDEIDLS
S16 SLDEIDLSALRDPAG
Y36 ELVGNGTYGQVYKGR
Y40 NGTYGQVYKGRHVkT
K46‑ub VYKGRHVkTGQLAAI
Y86 HRNIATYYGAFIKKN
K121 TDLIKNTKGNTLKEE
S175 KLVDFGVSAQLDRTV
T181 VSAQLDRTVGRRNtF
T187‑p RTVGRRNtFIGTPYW
S264 KWSKKFQSFIESCLV
T319 KRGEKDETEYEYSGS
Y321 GEKDETEYEYSGSEE
Y323 KDETEYEYSGSEEEE
S324 DETEYEYSGSEEEEE
S326 TEYEYSGSEEEEEEN
S335 EEEEENDSGEPSSIL
Y427 EREQRRHYEEQMRRE
Y475 EQALLLEYKRKQLEE
Y499 QLKQERDYLVSLQHQ
S502 QERDYLVSLQHQRQE
Y519 PLEKKPLYHYKEGMS
Y521 EKKPLYHYKEGMSPS
S526 YHYKEGMSPSEKPAW
S548 SRLNRQSSPAMPHKV
S560 HKVANRISDPNLPPR
S568 DPNLPPRSESFSISG
S568 DPNLPPRSESFSISG
S570 NLPPRSESFSISGVQ
S574 RSESFSISGVQPART
T581 SGVQPARTPPMLRPV
S600 PQLVAVKSQGPALTA
T606 KSQGPALTASQSVHE
S608 QGPALTASQSVHEQP
S610 PALTASQSVHEQPTK
S640‑p VEMPRQNsDPTSENP
T643 PRQNsDPTSENPPLP
S659 RIEKFDRSSWLRQEE
T677 PKVPQRTTsIsPALA
S678‑p KVPQRTTsIsPALAR
S680‑p PQRTTsIsPALARKN
S688 PALARKNSPGNGSAL
S701 ALGPRLGSQPIRAsN
S707‑p GSQPIRAsNPDLRRT
S720 RTEPVLESSLQRtSS
T725‑p LESSLQRtSSGSsss
S726 ESSLQRtSSGSsssS
S729 LQRtSSGSsssSSTP
S730‑p QRtSSGSsssSSTPS
S731‑p RtSSGSsssSSTPSS
S732‑p tSSGSsssSSTPSSQ
S755 PGSQAGSSERTRVRA
S764 RTRVRANSKSEGsPV
S766 RVRANSKSEGsPVLP
S769‑p ANSKSEGsPVLPHEP
S784‑p SKVKPEEsRDIPRPS
- gap
K816 EETNRPLKKVTDYSS
T894 GSISREGTLMIRETA
S930 DLVQQSHSPAGTPTE
T934 QSHSPAGTPTEGLGR
T951 THSQEMDTGAEyGMG
Y955‑p EMDTGAEyGMGSSTK
S964 MGSSTKASFTPFVDP
Y974‑p PFVDPRVyQTsPtDE
S977‑p DPRVyQTsPtDEDEE
T979‑p RVyQTsPtDEDEEDD
S988‑p EDEEDDEssAAALFt
S989‑p DEEDDEssAAALFtS
T995‑p ssAAALFtSELLRQE
S1013 LNEARKISVVNVNPT
T1028 NIRPHSDTPEIRKYK
S1108 KLRVYYLSWLRNRIL
T1276 LQWGEMPTSVAYIHS
S1277 QWGEMPTSVAYIHSN
V1343 CILRDHQVPVKPSFS
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