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Protein Page:
ULK1 (mouse)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitylation
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
ULK1 Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3- kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. Interacts with GABARAP and GABARAPL2. Interacts (via C- terminus) with ATG13/KIAA0652. Part of a complex consisting of ATG13/KIAA0652, ULK1 and RB1CC1. Associates with the mammalian target of rapamycin complex 1 (mTORC1) through an interaction with RPTOR; the association depends on nutrient conditions and is reduced during starvation. Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily. Note: This description may include information from UniProtKB.
Protein type: EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; Kinase, protein; Autophagy; Other group; ULK family
Cellular Component: autophagic vacuole; cell soma; cytoplasm; cytoplasmic vesicle membrane; cytosol; membrane; neuron projection; pre-autophagosomal structure membrane
Molecular Function: Hsp90 protein binding; protein binding; protein complex binding; protein kinase activity; protein kinase binding; protein serine/threonine kinase activity; Rab GTPase binding
Biological Process: autophagic vacuole formation; axon extension; axonogenesis; cellular response to amino acid starvation; cellular response to nutrient levels; cerebellar granule cell differentiation; macroautophagy; negative regulation of collateral sprouting; negative regulation of protein complex assembly; neurite development; neurite regeneration; peptidyl-serine phosphorylation; positive regulation of autophagy; positive regulation of macroautophagy; protein amino acid autophosphorylation; protein localization; radial glia guided migration of granule cell; Ras protein signal transduction; receptor internalization; regulation of autophagy; regulation of gene expression; regulation of nerve growth factor receptor signaling pathway; response to starvation
Reference #:  O70405 (UniProtKB)
Alt. Names/Synonyms: ATG1; AU041434; mKIAA0722; Serine/threonine-protein kinase ULK1; Serine/threonine-protein kinase Unc51.1; Ulk1; Unc-51 like kinase 1 (C. elegans); Unc-51-like kinase 1; Unc51.1
Gene Symbols: Ulk1
Molecular weight: 112,463 Da
Basal Isoelectric point: 8.93  Predict pI for various phosphorylation states
CST Pathways:  Autophagy Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
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ULK1

Protein Structure Not Found.
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Substrate Sequence Logo
Sequence Logo

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Sites Implicated In
autophagy, altered: S467‑p, S555‑p, T574‑p, S637‑p
autophagy, induced: S317‑p, S777‑p
molecular association, regulation: S757‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment



 LTP 

LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 HTP 

HTP: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       mouse

 
0 1 S87‑p DFQEMANsVYLVMEY
0 1 S111 LHTMRTLSEDTVRLF
0 1 S131 GAMRLLHSKGIIHRD
0 1 S147‑p KPQNILLsNPGGRRA
0 1 N158 GRRANPSNIRVkIAD
1 0 K162‑ac NPSNIRVkIADFGFA
1 0 T180‑p QSNMMAAtLCGSPMY
0 1 S195‑p MAPEVIMsQHYDGKA
0 1 S224‑p GKAPFQAsSPQDLRL
0 2 S225 KAPFQAsSPQDLRLF
0 2 S281‑p HHPFLDAstPIKKSP
0 1 T282‑p HPFLDAstPIKKSPP
7 1 S317‑p SHLASPPsLGEMPQL
0 1 S330 QLQKTLTSPADAAGF
0 1 S341‑p AAGFLQGsRDSGGSS
0 4 S403‑p ESHGRTPsPSPTCSS
0 1 S405 HGRTPsPSPTCSSSP
0 1 S409 PsPSPTCSSSPSPSG
0 3 S415 CSSSPSPSGRPGPFS
0 1 S429 SSNRYGASVPIPVPT
0 53 S450‑p RIEQNLQsPTQQQTA
0 19 T456 QsPTQQQTARssAIR
0 2 S459‑p TQQQTARssAIRRsG
0 2 S460‑p QQQTARssAIRRsGs
0 11 S465‑p RssAIRRsGsttPLG
3 23 S467‑p sAIRRsGsttPLGFG
0 5 T468‑p AIRRsGsttPLGFGR
0 35 T469‑p IRRsGsttPLGFGRA
0 15 S477‑p PLGFGRAsPsPPsHT
0 16 S479‑p GFGRAsPsPPsHTDG
0 1 S482‑p RAsPsPPsHTDGAML
0 4 S494‑p AMLARKLsLGGGRPY
0 5 S521‑p PSWSRVPsPQGADVR
0 3 S532‑p ADVRVGRsPRPGsSV
0 2 S537‑p GRsPRPGsSVPEHsP
0 1 S538 RsPRPGsSVPEHsPR
0 5 S543‑p GsSVPEHsPRTTGLG
14 52 S555‑p GLGCRLHsAPNLSDF
0 1 S560 LHsAPNLSDFHVVRP
0 9 T574‑p PKLPKPPtDPLGATF
0 2 S582 DPLGATFSPPQTSAP
0 9 S587 TFSPPQTSAPQPCPG
0 1 C592 QTSAPQPCPGLQsCR
0 2 S597‑p QPCPGLQsCRPLRGS
0 5 S604 sCRPLRGSPkLPDFL
1 0 K606‑ac RPLRGSPkLPDFLQR
0 5 S614‑p LPDFLQRsPLPPILG
0 26 S622‑p PLPPILGsPtKAGPS
0 6 T624‑p PPILGsPtKAGPSFD
0 7 T635‑p PSFDFPKtPssQNLL
1 67 S637‑p FDFPKtPssQNLLTL
0 12 S638‑p DFPKtPssQNLLTLL
0 3 T653 ARQGVVMTPPRNRtL
1 17 T659‑p MTPPRNRtLPDLSEA
0 1 S664 NRtLPDLSEASPFHG
0 5 S693‑p GPFGRSFsTSRITDL
0 5 T694 PFGRSFsTSRITDLL
0 3 T698 SFsTSRITDLLLKAA
0 1 T708 LLKAAFGTQASDsGs
0 1 S713‑p FGTQASDsGstDsLQ
0 3 S715‑p TQASDsGstDsLQEK
0 3 T716‑p QASDsGstDsLQEKP
0 6 S718‑p SDsGstDsLQEKPME
0 1 S746‑p GARGGGAssPAPVVF
0 2 S747‑p ARGGGAssPAPVVFT
25 16 S757‑p PVVFTVGsPPsGAtP
0 2 S760‑p FTVGsPPsGAtPPQS
0 3 T763‑p GsPPsGAtPPQSTRT
1 5 S774‑p STRTRMFsVGsSSsL
1 2 S777‑p TRMFsVGsSSsLGST
0 2 S780‑p FsVGsSSsLGSTGSS
0 1 S867‑p EIAALKGsASEAAGG
0 1 S913‑p KVAELLSsGLQTAID
0 5 S950‑p LYKASVVsCQGLSLR
1 2 S1043‑p LCIERRLsALLsGVY
2 1 S1047‑p RRLsALLsGVYA___
12753 : Phospho-ULK1 (Ser317) (D2B6Y) Rabbit mAb
4634 : Phospho-ULK1 (Ser467) Antibody
5869 : Phospho-ULK1 (Ser555) (D1H4) Rabbit mAb
12097 : Phospho-ULK1 (Ser638) Antibody
14205 : Phospho-ULK1 (Ser638) (D8K9O) Rabbit mAb
6888 : Phospho-ULK1 (Ser757) Antibody
14202 : Phospho-ULK1 (Ser757) (D7O6U) Rabbit mAb
  human

 
S87 DFQEMANSVYLVMEY
S111‑p LHAMRTLsEDTIRLF
S131‑p GAMRLLHsKGIIHRD
S147 KPQNILLSNPAGRRA
S158‑p GRRANPNsIRVKIAD
K162 NPNsIRVKIADFGFA
T180 QSNMMAATLCGSPMY
S195 MAPEVIMSQHYDGKA
S224 GKAPFQASsPQDLRL
S225‑p KAPFQASsPQDLRLF
S281‑p HHPFLDAsPSVRKSP
P282 HPFLDAsPSVRKSPP
S317‑p SHLASPPsLGEMQQL
S330‑p QLQKTLAsPADTAGF
S341 TAGFLHSSRDSGGSK
S403‑p ESHGRTPsPsPPCsS
S405‑p HGRTPsPsPPCsSSP
S409‑p PsPsPPCsSSPSPsG
S415‑p CsSSPSPsGRAGPFS
S429‑p SSSRCGAsVPIPVPT
S450‑p RIERNLQsPTQFQtP
T456‑p QsPTQFQtPRssAIR
S459‑p TQFQtPRssAIRRsG
S460‑p QFQtPRssAIRRsGs
S465‑p RssAIRRsGstsPLG
S467‑p sAIRRsGstsPLGFA
T468‑p AIRRsGstsPLGFAR
S469‑p IRRsGstsPLGFARA
S477‑p PLGFARAsPsPPAHA
S479‑p GFARAsPsPPAHAEH
A482 RAsPsPPAHAEHGGV
S495‑p GVLARKMsLGGGRPY
S522 PGWSGTPSPQGAEMR
S533‑p AEMRGGRsPRPGssA
S538‑p GRsPRPGssAPEHsP
S539‑p RsPRPGssAPEHsPR
S544‑p GssAPEHsPRTSGLG
S556‑p GLGCRLHsAPNLsDL
S561‑p LHsAPNLsDLHVVRP
T575‑p PKLPKPPtDPLGAVF
S583‑p DPLGAVFsPPQAsPP
S588‑p VFsPPQAsPPQPsHG
S593‑p QAsPPQPsHGLQsCR
S598‑p QPsHGLQsCRNLRGs
S605‑p sCRNLRGsPKLPDFL
K607 RNLRGsPKLPDFLQR
N615 LPDFLQRNPLPPILG
S623‑p PLPPILGsPtKAVPS
T625‑p PPILGsPtKAVPSFD
T636‑p PSFDFPKtPssQNLL
S638‑p FDFPKtPssQNLLAL
S639‑p DFPKtPssQNLLALL
T654‑p ARQGVVMtPPRNRtL
T660‑p MtPPRNRtLPDLsEV
S665‑p NRtLPDLsEVGPFHG
S694‑p GPFGRSFstSRLtDL
T695‑p PFGRSFstSRLtDLL
T699‑p SFstSRLtDLLLKAA
T709‑p LLKAAFGtQAPDPGs
P714 FGtQAPDPGstEsLQ
S716‑p tQAPDPGstEsLQEK
T717‑p QAPDPGstEsLQEKP
S719‑p PDPGstEsLQEKPME
S747 GARAGGTSsPSPVVF
S748‑p ARAGGTSsPSPVVFT
S758‑p PVVFTVGsPPsGStP
S761‑p FTVGsPPsGStPPQG
T764‑p GsPPsGStPPQGPRT
S775‑p GPRTRMFsAGPTGsA
P778 TRMFsAGPTGsASSS
S781‑p FsAGPTGsASSSARH
S866 EIAALKGSASEAAGG
S912 KVAELLSSGLQSAID
S949‑p LYKASVVsCQGLSLR
S1042‑p LCIERRLsALLtGIC
T1046‑p RRLsALLtGICA___
12753 : Phospho-ULK1 (Ser317) (D2B6Y) Rabbit mAb
4634 : Phospho-ULK1 (Ser467) Antibody
5869 : Phospho-ULK1 (Ser555) (D1H4) Rabbit mAb
12097 : Phospho-ULK1 (Ser638) Antibody
14205 : Phospho-ULK1 (Ser638) (D8K9O) Rabbit mAb
6888 : Phospho-ULK1 (Ser757) Antibody
14202 : Phospho-ULK1 (Ser757) (D7O6U) Rabbit mAb
  rat

 
S87 DFQEMANSVYLVMEY
S111 LHTMRTLSEDTVRLF
S131 GAMQLLHSKGIIHRD
S147 KPQNILLSNPGGRRA
N158 GRRANPSNIRVKIAD
K162 NPSNIRVKIADFGFA
T180 QSNMMAATLCGSPMY
S195 MAPEVIMSQHYDGKA
S224 GKAPFQASSPQDLRL
S225 KAPFQASSPQDLRLF
S281 HHPFLDASTPIKKSP
T282 HPFLDASTPIKKSPP
S317 SHLASPPSLGEMPQL
S330 QLQKTLTSPADAAGF
S341 AAGFLQGSRDSGGSS
S403 ESHGRTPSPSPTCSS
S405 HGRTPSPSPTCSSSP
S409 PSPSPTCSSSPSPSG
S415 CSSSPSPSGRPGPFS
S429 SSNRYGASVPIPVPT
S450‑p RIEQNLQsPTQQQTA
T456 QsPTQQQTARSSAIR
S459 TQQQTARSSAIRRSG
S460 QQQTARSSAIRRSGs
S465 RSSAIRRSGsTsPLG
S467‑p SAIRRSGsTsPLGFG
T468 AIRRSGsTsPLGFGR
S469‑p IRRSGsTsPLGFGRA
S477 PLGFGRASPSPPSHT
S479 GFGRASPSPPSHTDG
S482 RASPSPPSHTDGAML
S494 AMLARKLSLGGGRPY
S521‑p PSWSRVPsPQGADVR
S532 ADVRAGRSPRPGSSV
S537 GRSPRPGSSVPEHSP
S538 RSPRPGSSVPEHSPR
S543 GSSVPEHSPRTTGLG
S555 GLGCRLHSAPNLSDF
S560 LHSAPNLSDFHVVRP
T574 PKLPKPPTDPLGATF
S582 DPLGATFSPPQTSAP
S587 TFSPPQTSAPQPCPG
C592 QTSAPQPCPGLQSCR
S597 QPCPGLQSCRPLRGS
S604 SCRPLRGSPKLPDFL
K606 RPLRGSPKLPDFLQR
S614 LPDFLQRSPLPPILG
S622‑p PLPPILGsPTKAGPS
T624 PPILGsPTKAGPSFD
A635 PSFDFPKAPsSQSLL
S637‑p FDFPKAPsSQSLLTL
S638 DFPKAPsSQSLLTLL
T653‑p ARQGVVMtPPRNRTL
T659 MtPPRNRTLPDLSEA
S664 NRTLPDLSEAGPFQG
S693 GPFGRSFSTSRLTDL
T694 PFGRSFSTSRLTDLL
T698 SFSTSRLTDLLLKAA
T708 LLKAAFGTQASDSGS
S713 FGTQASDSGSTDsLQ
S715 TQASDSGSTDsLQEK
T716 QASDSGSTDsLQEKP
S718‑p SDSGSTDsLQEKPME
S746 GARAGGASSPAPVVF
S747 ARAGGASSPAPVVFT
S757 PVVFTVGSPPSGTTP
S760 FTVGSPPSGTTPPQS
T763 GSPPSGTTPPQSTRT
S774 STRTRMFSVGSSSSL
S777 TRMFSVGSSSSLGST
S780 FSVGSSSSLGSTGSS
S867 EIAALKGSASEAAGG
S913 KVAELLSSGLQTAID
S950 LYKASVVSCQGLSLR
S1043 LCIERRLSALLSGVY
S1047 RRLSALLSGVYA___
4634 : Phospho-ULK1 (Ser467) Antibody
5869 : Phospho-ULK1 (Ser555) (D1H4) Rabbit mAb
14202 : Phospho-ULK1 (Ser757) (D7O6U) Rabbit mAb
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