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Protein Page:
GABARAPL2 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitylation
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
GABARAPL2 is a ubiquitin-like protein that is a constituent of the ATG8-conjugation system, one of two evolutionarily conserved phosphatidylethanolamine conjugation systems necessary for the formation of the autophagosome. The human ATG8 system includes seven ubiquitin-like light chain proteins (LCPs) that are homologs of yeast LC3: MAP1LC3A, -B, -C, GABARAP, GABARAPL1, -2, and -3. Pro-LCPs are cleaved by ATG4B to expose a C-terminal glycine residue, the cytosolic LCP-I form. The exposed C-terminus is conjugated to the head group amine of phosphatidylethanolamine (PE) through an amide bond by a sequence of ubiquitination-like reactions that involves an E1 (ATG7), an E2 (ATG3), and an E3 (a complex including ATG5, ATG12, and ATG16L). The PE-congugated form (LCP-II) is tightly associated with the autophagosomal membrane. The LCP-II forms can also be delipidated by the ATG4 proteases: most of the LCPs are delipidated and liberated from the membrane before autophagosomes fuse with lysosomes. Implicated in intra-Golgi transport and post-mitotic Golgi re-assembly. Modulates the activity of SNAREs in the Golgi apparatus and is therefore an essential component of intra-Golgi transport and post-mitotic Golgi re-assembly. It first stimulates the ATPase activity of NSF, which in turn stimulates the association with GOSR1. Interacts with GABRG2, NSF, GOSR1 and beta-tubulin. Interacts with ULK1. Phosphorylated upon DNA damage, probably by ATM or ATR. Note: This description may include information from UniProtKB.
Protein type: Ubiquitin-like modifier; Adaptor/scaffold; Autophagy; Microtubule-binding; Vesicle
Chromosomal Location of Human Ortholog: 16q22.1
Cellular Component: autophagic vacuole; cytoplasm; cytosol; Golgi apparatus; Golgi membrane; intracellular
Molecular Function: ATPase binding; beta-tubulin binding; GABA receptor binding; protein binding; SNARE binding; ubiquitin protein ligase binding
Biological Process: autophagic vacuole formation; cellular response to nitrogen starvation; intra-Golgi vesicle-mediated transport; macroautophagy; mitochondrion degradation; positive regulation of ATPase activity
Reference #:  P60520 (UniProtKB)
Alt. Names/Synonyms: ATG8; FLC3A; GABA(A) receptor-associated protein-like 2; GABARAPL2; Gamma-aminobutyric acid receptor-associated protein-like 2; Ganglioside expression factor 2; GATE-16; GATE16; GBRL2; GEF-2; GEF2; General protein transport factor p16; Golgi-associated ATPase enhancer of 16 kDa; MAP1 light chain 3 related protein; MAP1 light chain 3-related protein
Gene Symbols: GABARAPL2
Molecular weight: 13,667 Da
Basal Isoelectric point: 7.81  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

GABARAPL2

Protein Structure Not Found.
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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment



 LTP 

LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 HTP 

HTP: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 4 S10‑p WMFKEDHsLEHRCVE
0 2 K24‑ac ESAKIRAkYPDRVPV
0 3 K24‑ub ESAKIRAkYPDRVPV
0 4 K35‑ac RVPVIVEkVSGsQIV
0 1 S39‑p IVEkVSGsQIVDIDk
0 1 K46‑ac sQIVDIDkRKYLVPS
0 2 K74‑ac RIQLPSEkAIFLFVD
0 2 K74 RIQLPSEKAIFLFVD
  mouse

 
S10‑p WMFKEDHsLEHRCVE
K24 ESAKIRAKYPDRVPV
K24‑ub ESAKIRAkYPDRVPV
K35‑ac RVPVIVEkVSGSQIV
S39 IVEkVSGSQIVDIDK
K46 SQIVDIDKRKYLVPS
K74 RIQLPSEKAIFLFVD
K74‑ub RIQLPSEkAIFLFVD
  rat

 
S10 WMFKEDHSLEHRCVE
K24 ESAKIRAKYPDRVPV
K24 ESAKIRAKYPDRVPV
K35 RVPVIVEKVSGSQIV
S39 IVEKVSGSQIVDIDK
K46 SQIVDIDKRKYLVPS
K74 RIQLPSEKAIFLFVD
K74 RIQLPSEKAIFLFVD
  cow

 
S10 WMFKEDHSLEHRCVE
K24 ESAKIRAKYPDRVPV
K24 ESAKIRAKYPDRVPV
K35 RVPVIVEKVSGSQIV
S39 IVEKVSGSQIVDIDK
K46 SQIVDIDKRKYLVPS
K74 RIQLPSEKAIFLFVD
K74 RIQLPSEKAIFLFVD
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