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Protein Page:
SMAD3 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitylation
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
SMAD3 transcription factor phosphorylated and activated by TGF-beta-type receptors. A receptor-regulated Smad (R-smad). Binds directly to consensus DNA-binding elements in the promoters of target genes. In mouse required for establishemnt of the mucosal immune response and proper development of skeleton. Note: This description may include information from UniProtKB.
Protein type: DNA-binding; Transcription factor; Nuclear receptor co-regulator
Chromosomal Location of Human Ortholog: 15q22.33
Cellular Component: cytoplasm; cytosol; nuclear chromatin; nuclear inner membrane; nucleoplasm; nucleus; plasma membrane; receptor complex; transcription factor complex
Molecular Function: beta-catenin binding; bHLH transcription factor binding; chromatin DNA binding; collagen binding; identical protein binding; phosphatase binding; protein binding; protein heterodimerization activity; protein homodimerization activity; protein kinase binding; sequence-specific DNA binding; transcription factor activity; transcription factor binding; transforming growth factor beta receptor binding; transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity; ubiquitin binding; ubiquitin protein ligase binding; zinc ion binding
Biological Process: activin receptor signaling pathway; caspase activation; cell cycle arrest; developmental growth; embryonic cranial skeleton morphogenesis; embryonic foregut morphogenesis; embryonic pattern specification; endoderm development; evasion of host defenses by virus; heart looping; immune response; immune system development; in utero embryonic development; intercellular junction assembly and maintenance; liver development; mesoderm formation; negative regulation of apoptosis; negative regulation of cell growth; negative regulation of fat cell differentiation; negative regulation of inflammatory response; negative regulation of mitotic cell cycle; negative regulation of osteoblast differentiation; negative regulation of osteoblast proliferation; negative regulation of protein amino acid phosphorylation; negative regulation of protein catabolic process; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transforming growth factor beta receptor signaling pathway; osteoblast development; paraxial mesoderm morphogenesis; pericardium development; positive regulation of bone mineralization; positive regulation of cell migration; positive regulation of chondrocyte differentiation; positive regulation of focal adhesion formation; positive regulation of interleukin-1 beta production; positive regulation of positive chemotaxis; positive regulation of stress fiber formation; positive regulation of transcription factor import into nucleus; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; positive regulation of transforming growth factor-beta3 production; primary microRNA processing; protein stabilization; regulation of binding; regulation of epithelial cell proliferation; regulation of immune response; regulation of striated muscle development; regulation of transcription from RNA polymerase II promoter; regulation of transforming growth factor beta receptor signaling pathway; regulation of transforming growth factor-beta2 production; response to hypoxia; SMAD protein complex assembly; somitogenesis; T cell activation; thyroid gland development; transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; transport; ureteric bud development; wound healing
Disease: Loeys-dietz Syndrome 3
Reference #:  P84022 (UniProtKB)
Alt. Names/Synonyms: DKFZp586N0721; DKFZp686J10186; hMAD-3; hSMAD3; HSPC193; HsT17436; JV15-2; MAD homolog 3; mad homolog JV15-2; mad protein homolog; MAD, mothers against decapentaplegic homolog 3; Mad3; MADH3; MGC60396; Mothers against decapentaplegic homolog 3; Mothers against DPP homolog 3; SMA- and MAD-related protein 3; SMAD 3; SMAD family member 3; SMAD, mothers against DPP homolog 3; SMAD3
Gene Symbols: SMAD3
Molecular weight: 48,081 Da
Basal Isoelectric point: 6.73  Predict pI for various phosphorylation states
CST Pathways:  Angiogenesis  |  ESC Pluripotency and Differentiation  |  G1/S Checkpoint  |  TGF-ß Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

SMAD3

Protein Structure Not Found.
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Sites Implicated In
cell cycle regulation: T8‑p, T179‑p, S213‑p
cell growth, altered: T179‑p, S204‑p, S208‑p, S213‑p
cell motility, altered: S423‑p, S425‑p
cell motility, induced: T179‑p
signaling pathway regulation: S204‑p
transcription, altered: T8‑p, T179‑p, S204‑p, S208‑p, S213‑p, S422‑p, S423‑p, S425‑p
transcription, induced: T388‑p
transcription, inhibited: T8‑p, T179‑p, S204‑p, S208‑p, S213‑p, T388‑p
activity, induced: S423‑p, S425‑p
activity, inhibited: T179‑p, S204‑p, S208‑p
intracellular localization: T179‑p, S204‑p, S208‑p, S213‑p, S416‑p, S418‑p
molecular association, regulation: T179‑p, S204‑p, T388‑p
protein conformation: S423‑p, S425‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 LTP 

LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 HTP 

HTP: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
5 10 T8‑p MSSILPFtPPIVkRL
0 1 K13‑ub PFtPPIVkRLLGWkK
0 5 K19‑ac VkRLLGWkKGEQNGQ
0 1 K29‑ac EQNGQEEkWCEKAVK
0 6 T132‑p YHYQRVEtPVLPPVL
12 0 T179‑p PQSNIPEtPPPGYLS
13 0 S204‑p NHSMDAGsPNLsPNP
13 0 S208‑p DAGsPNLsPNPMsPA
10 0 S213‑p NLsPNPMsPAHNNLD
2 0 S309‑p EVFAECLsDSAIFVQ
1 3 K378‑ac TIRMSFVkGWGAEYR
3 0 T388‑p GAEYRRQtVTSTPCW
1 3 S416‑p KVLTQMGsPsIRCss
2 0 S418‑p LTQMGsPsIRCssVs
2 0 S422‑p GsPsIRCssVs____
20 5 S423‑p sPsIRCssVs_____
21 5 S425‑p sIRCssVs_______
8769 : Phospho-Smad3 (Ser423/425) (D12E11) Rabbit mAb
8828 : Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb
9510 : Phospho-Smad2 (Ser465/467)/ Smad3 (Ser423/425) (D6G10) XP(R) Rabbit mAb
9520 : Phospho-Smad3 (Ser423/425) (C25A9) Rabbit mAb
8769 : Phospho-Smad3 (Ser423/425) (D12E11) Rabbit mAb
8828 : Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb
9510 : Phospho-Smad2 (Ser465/467)/ Smad3 (Ser423/425) (D6G10) XP(R) Rabbit mAb
9520 : Phospho-Smad3 (Ser423/425) (C25A9) Rabbit mAb
  mouse

 
T8‑p MSSILPFtPPIVKRL
K13 PFtPPIVKRLLGWKK
K19 VKRLLGWKKGEQNGQ
K29 EQNGQEEKWCEKAVK
T132‑p YHYQRVEtPVLPPVL
T179 PQSNIPETPPPGYLS
S204 NHSMDAGSPNLSPNP
S208 DAGSPNLSPNPMSPA
S213 NLSPNPMSPAHNNLD
S309‑p EVFAECLsDSAIFVQ
K378 TIRMSFVKGWGAEYR
T388‑p GAEYRRQtVTSTPCW
S416 KVLTQMGSPSIRCSs
S418 LTQMGSPSIRCSsVs
S422 GSPSIRCSsVs____
S423‑p SPSIRCSsVs_____
S425‑p SIRCSsVs_______
8769 : Phospho-Smad3 (Ser423/425) (D12E11) Rabbit mAb
8828 : Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb
9510 : Phospho-Smad2 (Ser465/467)/ Smad3 (Ser423/425) (D6G10) XP(R) Rabbit mAb
9520 : Phospho-Smad3 (Ser423/425) (C25A9) Rabbit mAb
8769 : Phospho-Smad3 (Ser423/425) (D12E11) Rabbit mAb
8828 : Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb
9510 : Phospho-Smad2 (Ser465/467)/ Smad3 (Ser423/425) (D6G10) XP(R) Rabbit mAb
9520 : Phospho-Smad3 (Ser423/425) (C25A9) Rabbit mAb
  rat

 
T8‑p MSSILPFtPPIVKRL
K13 PFtPPIVKRLLGWKK
K19 VKRLLGWKKGEQNGQ
K29 EQNGQEEKWCEKAVK
T132 YHYQRVETPVLPPVL
T179 PQSNIPETPPPGYLS
S204 NHSMDAGSPNLSPNP
S208 DAGSPNLSPNPMSPA
S213 NLSPNPMSPAHNNLD
S309 EVFAECLSDSAIFVQ
K378 TIRMSFVKGWGAEYR
T388 GAEYRRQTVTSTPCW
S416 KVLTQMGSPSIRCSs
S418 LTQMGSPSIRCSsVs
S422 GSPSIRCSsVs____
S423‑p SPSIRCSsVs_____
S425‑p SIRCSsVs_______
8769 : Phospho-Smad3 (Ser423/425) (D12E11) Rabbit mAb
8828 : Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb
9510 : Phospho-Smad2 (Ser465/467)/ Smad3 (Ser423/425) (D6G10) XP(R) Rabbit mAb
9520 : Phospho-Smad3 (Ser423/425) (C25A9) Rabbit mAb
8769 : Phospho-Smad3 (Ser423/425) (D12E11) Rabbit mAb
8828 : Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb
9510 : Phospho-Smad2 (Ser465/467)/ Smad3 (Ser423/425) (D6G10) XP(R) Rabbit mAb
9520 : Phospho-Smad3 (Ser423/425) (C25A9) Rabbit mAb
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