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Protein Page:
nNOS (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitylation
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
nNOS nitric oxide synthase 1, neuronal form. Produces nitric oxide which is a messenger molecule with diverse functions and displays many properties of a neurotransmitter in the brain and peripheral nervous system. May be an effector enzyme for the dystrophin complex. Four alternatively spliced isoforms have been described. Note: This description may include information from UniProtKB.
Protein type: EC 1.14.13.39; Amino Acid Metabolism - arginine and proline; Oxidoreductase
Chromosomal Location of Human Ortholog: 12q24.22
Cellular Component: cytoplasm; cytoskeleton; cytosol; lipid raft; mitochondrion; perinuclear region of cytoplasm; photoreceptor inner segment; protein complex; sarcolemma; sarcoplasmic reticulum; synapse
Molecular Function: cadmium ion binding; FAD binding; FMN binding; heme binding; NADP binding; nitric-oxide synthase activity; protein binding; sodium channel regulator activity
Biological Process: arginine catabolic process; cell redox homeostasis; exogenous drug catabolic process; multicellular organismal response to stress; myoblast fusion; negative regulation of blood pressure; negative regulation of calcium ion transport; negative regulation of hydrolase activity; negative regulation of potassium ion transport; negative regulation of serotonin uptake; neurotransmitter biosynthetic process; nitric oxide biosynthetic process; nitric oxide mediated signal transduction; peptidyl-cysteine S-nitrosylation; positive regulation of guanylate cyclase activity; positive regulation of histone acetylation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; positive regulation of vasodilation; regulation of sodium ion transport; response to heat; response to hypoxia; striated muscle contraction
Disease: Pyloric Stenosis, Infantile Hypertrophic, 1
Reference #:  P29475 (UniProtKB)
Alt. Names/Synonyms: bNOS; Constitutive NOS; IHPS1; N-NOS; NC-NOS; Neuronal NOS; nitric oxide synthase 1 (neuronal); Nitric oxide synthase, brain; nNOS; NOS; NOS type I; NOS1
Gene Symbols: NOS1
Molecular weight: 160,970 Da
Basal Isoelectric point: 7.1  Predict pI for various phosphorylation states
CST Pathways:  Angiogenesis  |  Growth And Differentiation Control by MAPKs
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

nNOS

Protein Structure Not Found.
Download PyMol Script
Download ChimeraX Script


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Sites Implicated In
enzymatic activity, inhibited: Y609‑p
molecular association, regulation: Y609‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment



 LTP 

LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 HTP 

HTP: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
1 0 K24 SVRLFKRKVGGLGFL
1 1 K24 SVRLFKRKVGGLGFL
2 0 K33 GGLGFLVKERVSKPP
1 0 K38 LVKERVSKPPVIISD
0 1 Y77 RPLVDLSYDSALEVL
0 1 P128 RVTQPLGPPTKAVDL
0 1 T130 TQPLGPPTKAVDLSH
1 0 K131 QPLGPPTKAVDLSHQ
0 1 K131 QPLGPPTKAVDLSHQ
0 1 P140 VDLSHQPPAGKEQPL
1 0 K143 SHQPPAGKEQPLAVD
1 0 R193 DPAKKATRVSLQGRG
0 1 R221 SLLTSGSRGVKGGAP
1 0 K230 VKGGAPAKAEMKDMG
1 0 K234 APAKAEMKDMGIQVD
1 0 K234 APAKAEMKDMGIQVD
1 0 K250 DLDGKSHKPLPLGVE
1 0 K250 DLDGKSHKPLPLGVE
0 1 P285 PYSEKEQPPTSGKQs
0 2 S292‑p PPTSGKQsPTKNGSP
1 0 K375 DQYYSSIKRFGSKAH
1 0 K391 ERLEEVNKEIDttsT
0 1 T395‑p EVNKEIDttsTYQLK
0 1 T396‑p VNKEIDttsTYQLKD
0 1 S397‑p NKEIDttsTYQLKDT
0 1 Y453‑p YICNHVKyATNKGNL
1 0 Y609‑p DYCDNSRyNILEEVA
0 1 T724‑p NTHVWKGtNGTPTKR
1 1 K744 KKLAEAVKFSAKLMG
1 0 S746 LAEAVKFSAKLMGQA
1 0 K748 EAVKFSAKLMGQAMA
1 0 K759 QAMAKRVKATILYAt
0 1 Y764 RVKATILYAtEtGKS
0 2 T766‑p KATILYAtEtGKSQA
0 2 T768‑p TILYAtEtGKSQAYA
11 6 S852‑p SYKVRFNsVSSYsDs
0 1 S854 KVRFNsVSSYsDsQK
0 1 S857‑p FNsVSSYsDsQKSSG
0 2 S859‑p sVSSYsDsQKSSGDG
1 0 K861 SSYsDsQKSSGDGPD
0 1 S862 SYsDsQKSSGDGPDL
0 3 S863 YsDsQKSSGDGPDLR
0 1 S885‑p PLANVRFsVFGLGSR
1 0 K994 QGLSNVHKKRVSAAR
1 0 T1301 DHIYREETLQAKNKG
1 0 K1305 REETLQAKNKGVFRE
1 0 K1326 REPDKPKKYVQDILQ
0 1 T1358‑p IYVCGDVtMAADVLK
1 0 K1376 RIMTQQGKLsAEDAG
1 0 K1376 RIMTQQGKLsAEDAG
0 1 S1378‑p MTQQGKLsAEDAGVF
0 1 S1387‑p EDAGVFIsRMRDDNR
7 0 S1417‑p TNRLRSEsIAFIEES
  nNOS iso2  
K24 SVRLFKRKVGGLGFL
K24 SVRLFKRKVGGLGFL
K33 GGLGFLVKERVSKPP
K38 LVKERVSKPPVIISD
Y77 RPLVDLSYDSALEVL
P128 RVTQPLGPPTKAVDL
T130 TQPLGPPTKAVDLSH
K131 QPLGPPTKAVDLSHQ
K131 QPLGPPTKAVDLSHQ
P140 VDLSHQPPAGKEQPL
K143 SHQPPAGKEQPLAVD
R193 DPAKKATRVSLQGRG
R221 SLLTSGSRGVKGGAP
K230 VKGGAPAKAEMKDMG
K234 APAKAEMKDMGIQVD
K234 APAKAEMKDMGIQVD
K250 DLDGKSHKPLPLGVE
K250 DLDGKSHKPLPLGVE
P285 PYSEKEQPPTSGKQS
S292 PPTSGKQSPTKNGSP
K375 DQYYSSIKRFGSKAH
K391 ERLEEVNKEIDTTST
T395 EVNKEIDTTSTYQLK
T396 VNKEIDTTSTYQLKD
S397 NKEIDTTSTYQLKDT
Y453 YICNHVKYATNKGNL
- gap
T619 NTHVWKGTNGTPTKR
K639 KKLAEAVKFSAKLMG
S641 LAEAVKFSAKLMGQA
K643 EAVKFSAKLMGQAMA
K654 QAMAKRVKATILYAT
Y659 RVKATILYATETGKS
T661 KATILYATETGKSQA
T663 TILYATETGKSQAYA
S747 SYKVRFNSVSSYSDS
S749 KVRFNSVSSYSDSQK
S752 FNSVSSYSDSQKSSG
S754 SVSSYSDSQKSSGDG
K756 SSYSDSQKSSGDGPD
S757 SYSDSQKSSGDGPDL
S758 YSDSQKSSGDGPDLR
S780 PLANVRFSVFGLGSR
K889 QGLSNVHKKRVSAAR
T1196 DHIYREETLQAKNKG
K1200 REETLQAKNKGVFRE
K1221 REPDKPKKYVQDILQ
T1253 IYVCGDVTMAADVLK
K1271 RIMTQQGKLSAEDAG
K1271 RIMTQQGKLSAEDAG
S1273 MTQQGKLSAEDAGVF
S1282 EDAGVFISRMRDDNR
S1312 TNRLRSESIAFIEES
  mouse

► Hide Isoforms
 
K24 SVRLFKRKVGGLGFL
K24‑ub SVRLFKRkVGGLGFL
K33 GGLGFLVKERVSKPP
K38 LVKERVSKPPVIISD
Y77 RPLVDLSYDSALEVL
T128‑p RVTQPLGtPtkAVDL
T130‑p TQPLGtPtkAVDLSR
K131 QPLGtPtKAVDLSRQ
K131‑ub QPLGtPtkAVDLSRQ
S140‑p VDLSRQPsASKDQPL
K143 SRQPsASKDQPLAVD
K188 DPTMKNTKANLQDSG
K216‑ub SILTGGGkAVNRGGP
K225 VNRGGPAKAEMKDTG
K229 GPAKAEMKDTGIQVD
K229 GPAKAEMKDTGIQVD
K245 DLDGKLHKAPPLGGE
K245 DLDGKLHKAPPLGGE
S280‑p PYSENEQsPASGKQs
S287‑p sPASGKQsPTKNGSP
K370 DQYYSSIKRFGSKAH
K386 DRLEEVNKEIESTST
S390 EVNKEIESTSTYQLK
T391 VNKEIESTSTYQLKD
S392 NKEIESTSTYQLKDT
Y448 YICNHVKYATNKGNL
Y604 DYCDNSRYNILEEVA
T719 NTHVWKGTNGTPTKR
K739‑ub KKLAEAVkFSAKLMG
S741 LAEAVkFSAKLMGQA
K743 EAVkFSAKLMGQAMA
K754 QAMAKRVKATILyAt
Y759‑p RVKATILyAtEtGKS
T761‑p KATILyAtEtGKSQA
T763‑p TILyAtEtGKSQAYA
S847‑p SYKVRFNsVsSYSDs
S849‑p KVRFNsVsSYSDsRK
S852 FNsVsSYSDsRKssG
S854‑p sVsSYSDsRKssGDG
K856 sSYSDsRKssGDGPD
S857‑p SYSDsRKssGDGPDL
S858‑p YSDsRKssGDGPDLR
S880 PLANVRFSVFGLGSR
K989 QGLSNVHKKRVSAAR
T1296 DHIYREETLQAKNKG
K1300 REETLQAKNKGVFRE
K1321 REPDRPKKYVQDVLQ
T1353 IYVCGDVTMAADVLK
K1371 RIMTQQGKLSEEDAG
K1371 RIMTQQGKLSEEDAG
S1373 MTQQGKLSEEDAGVF
S1382 EDAGVFISRLRDDNR
S1412‑p TNRLRSEsIAFIEES
  nNOS iso5  
K24 SVRLFKRKVGGLGFL
K24 SVRLFKRKVGGLGFL
K33 GGLGFLVKERVSKPP
K38 LVKERVSKPPVIISD
Y77 RPLVDLSYDSALEVL
T128 RVTQPLGTPTKAVDL
T130 TQPLGTPTKAVDLSR
K131 QPLGTPTKAVDLSRQ
K131 QPLGTPTKAVDLSRQ
S140 VDLSRQPSASKDQPL
K143 SRQPSASKDQPLAVD
K188 DPTMKNTKANLQDSG
K216 SILTGGGKAVNRGGP
K225 VNRGGPAKAEMKDTG
K229 GPAKAEMKDTGIQVD
K229 GPAKAEMKDTGIQVD
K245 DLDGKLHKAPPLGGE
K245 DLDGKLHKAPPLGGE
S280 PYSENEQSPASGKQS
S287 SPASGKQSPTKNGSP
K370 DQYYSSIKRFGSKAH
K386 DRLEEVNKEIESTST
S390 EVNKEIESTSTYQLK
T391 VNKEIESTSTYQLKD
S392 NKEIESTSTYQLKDT
Y448 YICNHVKYATNKGNL
Y604 DYCDNSRYNILEEVA
T719 NTHVWKGTNGTPTKR
K739 KKLAEAVKFSAKLMG
S741 LAEAVKFSAKLMGQA
K743 EAVKFSAKLMGQAMA
K754 QAMAKRVKATILYAT
Y759 RVKATILYATETGKS
T761 KATILYATETGKSQA
T763 TILYATETGKSQAYA
S881 SYKVRFNSVSSYSDS
S883 KVRFNSVSSYSDSRK
S886 FNSVSSYSDSRKSSG
S888 SVSSYSDSRKSSGDG
K890 SSYSDSRKSSGDGPD
S891 SYSDSRKSSGDGPDL
S892 YSDSRKSSGDGPDLR
S914 PLANVRFSVFGLGSR
K1023 QGLSNVHKKRVSAAR
T1330 DHIYREETLQAKNKG
K1334 REETLQAKNKGVFRE
K1355 REPDRPKKYVQDVLQ
T1387 IYVCGDVTMAADVLK
K1405 RIMTQQGKLSEEDAG
K1405 RIMTQQGKLSEEDAG
S1407 MTQQGKLSEEDAGVF
S1416 EDAGVFISRLRDDNR
S1446‑p TNRLRSEsIAFIEES
  rat

 
K24‑ac SVRLFKRkVGGLGFL
K24‑ub SVRLFKRkVGGLGFL
K33‑ac GGLGFLVkERVSkPP
K38‑ac LVkERVSkPPVIISD
Y77‑p RPLVDLSyDSALEVL
P128 RVTQPLGPPTkAVDL
T130