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IkB-alpha (human)

Overview IkB-alpha a regulatory protein that inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. May be involved in regulation of transcriptional responses to NF-kappa-B, including cell adhesion, immune and proinflammatory responses, apoptosis, differentiation and growth. Controlled by sequential serine-phosphorylation, ubiquitination and degradation. Note: This description may include information from UniProtKB. Protein type: DNA binding protein; Inhibitor protein Cellular Component: cytoplasm; plasma membrane; cytosol; nucleus Molecular Function: identical protein binding; NF-kappaB binding; protein binding; enzyme binding; heat shock protein binding; ubiquitin protein ligase binding; nuclear localization sequence binding; transcription factor binding Biological Process: nerve growth factor receptor signaling pathway; apoptosis; positive regulation of cellular protein metabolic process; toll-like receptor 3 signaling pathway; T cell receptor signaling pathway; activation of NF-kappaB transcription factor; response to exogenous dsRNA; cytoplasmic sequestering of NF-kappaB; positive regulation of interferon type I production; lipopolysaccharide-mediated signaling pathway; toll-like receptor 4 signaling pathway; cytoplasmic sequestering of transcription factor; negative regulation of myeloid cell differentiation; MyD88-independent toll-like receptor signaling pathway; negative regulation of DNA binding; Toll signaling pathway; regulation of NF-kappaB import into nucleus; toll-like receptor 1 signaling pathway; regulation of cell proliferation; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; response to muramyl dipeptide; inhibition of NF-kappaB transcription factor; protein import into nucleus, translocation; virus-host interaction; toll-like receptor signaling pathway; innate immune response; negative regulation of Notch signaling pathway; positive regulation of transcription from RNA polymerase II promoter Reference #:  P25963 (UniProtKB) Alt. Names/Synonyms: I-kappa-B-alpha; IkappaBalpha; IkB-alpha; IKBA; MAD-3; MAD3; Major histocompatibility complex enhancer-binding protein MAD3; NF-kappa-B inhibitor alpha; NFKBI; NFKBIA; nuclear factor of kappa light chain gene enhancer in B-cells; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha Gene Symbols: NFKBIA Molecular weight: 35,609 Da Basal Isoelectric point: 4.57  Predict pI for various phosphorylation states CST Pathways:  Apoptosis  |  B Cell Receptor Signaling  |  Death Receptor Signaling  |  Inhibition of Apoptosis  |  MAPK/Erk in Growth and Differentiation  |  NF-kB Signaling  |  T Cell Receptor Signaling  |  Toll-like Receptors Pathway Protein-Specific Antibodies or siRNAs from Cell Signaling Technology®

Select Structure to View Below IkB-alpha 1IKN - D=67-302 (human) 1NFI - E/F=70-282 (human)

STRING  |  Scansite  |  Phospho.ELM  |  NetworKIN  |  Pfam  |  RCSB PDB 1IKN 1NFI  |  Phospho3D  |  DISEASE 164008 612132  |  Source  |  InnateDB  |  UCSD-Nature  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene

	Sites Implicated In
apoptosis, altered: S32‑p, S36‑p apoptosis, induced: S32‑p, S36‑p apoptosis, inhibited: S32‑p, S36‑p, Y42‑p transcription, altered: S32‑p, S36‑p, Y42‑p activation: S32‑p, S36‑p, Y42‑p intracellular localization: S32‑p, S36‑p molecular association, regulation: Y42‑p phosphorylation: S32‑p, S36‑p protein degradation: S32‑p, S36‑p, Y42‑p, S283‑p, T291‑p, S293‑p, T299‑p, Y305‑p

	Modification Sites and Domains	Show Modification Legend
Click here to view phosphorylation modifications only

	Modification Sites in Parent Protein, Orthologs, and Isoforms	Show Modification Legend

SS  SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.	MS  MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.			human
4	0		K21-u	EGPRDGLkkERLLDD
4	0		K21-s	EGPRDGLkkERLLDD
4	0		K22-u	GPRDGLkkERLLDDR
1	0		K22-s	GPRDGLkkERLLDDR
119	8		S32-p	LLDDRHDsGLDsMkD
81	6		S36-p	RHDsGLDsMkDEEyE
1	0		K38-u	DsGLDsMkDEEyEQM
14	26		Y42-p	DsMkDEEyEQMVkEL
1	0		K47-u	EEyEQMVkELQEIRL
0	4		K67-u	PRGSEPWkQQLTEDG
0	1		K87-u	LAIIHEEkALtMEVI
0	1		T90-p	IHEEkALtMEVIRQV
0	2		K98-u	MEVIRQVkGDLAFLN
0	8		K238-u	DLVSLLLkCGADVNR
0	1		Y248	ADVNRVTYQGYSPYQ
0	1		Y251	NRVTYQGYSPYQLTW
0	1		S252	RVTYQGYSPYQLTWG
3	0		S283-p	NLQMLPEsEDEEsYD
1	0		S288-p	PEsEDEEsYDtEsEF
4	0		T291-p	EDEEsYDtEsEFTEF
3	0		S293-p	EEsYDtEsEFTEFtE
2	0		T299-p	EsEFTEFtEDELPyD
2	3		Y305-p	FtEDELPyDDCVFGG

	mouse
K21-u	EGPRDGLkkERLVDD
K21-s	EGPRDGLkkERLVDD
K22-u	GPRDGLkkERLVDDR
K22	GPRDGLkKERLVDDR
S32-p	LVDDRHDsGLDsMKD
S36-p	RHDsGLDsMKDEEYE
K38	DsGLDsMKDEEYEQM
Y42	DsMKDEEYEQMVKEL
K47	EEYEQMVKELREIRL
K67	PLAAEPWKQQLTEDG
K87	LAIIHEEKPLTMEVI
T90	IHEEKPLTMEVIGQV
K98	MEVIGQVKGDLAFLN
K238	DLVSLLLKCGADVNR
Y248-p	ADVNRVTyQGysPYQ
Y251-p	NRVTyQGysPYQLTW
S252-p	RVTyQGysPYQLTWG
S283	NLQMLPESEDEESYD
S288	PESEDEESYDTEsEF
T291	EDEESYDTEsEFTED
S293-p	EESYDTEsEFTEDEL
T296	YDTEsEFTEDELPYD
Y302	FTEDELPYDDCVFGG

	rat
K21	EGPRDGLKKERLVDD
K21	EGPRDGLKKERLVDD
K22	GPRDGLKKERLVDDR
K22	GPRDGLKKERLVDDR
S32-p	LVDDRHDsGLDsMKD
S36-p	RHDsGLDsMKDEDyE
K38	DsGLDsMKDEDyEQM
Y42-p	DsMKDEDyEQMVKEL
K47	EDyEQMVKELREIRL
K67	PLAAEPWKQQLTEDG
K87	LAIIHEEKTLTMEVI
T90	IHEEKTLTMEVIGQV
K98	MEVIGQVKGDLAFLN
K238	DLVSLLLKCGADVNR
Y248	ADVNRVTYQGYSPYQ
Y251	NRVTYQGYSPYQLTW
S252	RVTYQGYSPYQLTWG
S283	NLQTLPESEDEESYD
S288	PESEDEESYDTESEF
T291	EDEESYDTESEFTED
S293	EESYDTESEFTEDEL
T296	YDTESEFTEDELPYD
Y302	FTEDELPYDDCVFGG

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