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Protein Page:
IkB-alpha (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
IkB-alpha a regulatory protein that inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. May be involved in regulation of transcriptional responses to NF-kappa-B, including cell adhesion, immune and proinflammatory responses, apoptosis, differentiation and growth. Controlled by sequential serine-phosphorylation, ubiquitination and degradation. Note: This description may include information from UniProtKB.
Protein type: Inhibitor protein; DNA binding protein
Chromosomal Location of Human Ortholog: 14q13
Cellular Component: cytoplasm; plasma membrane; cytosol; nucleus
Molecular Function: identical protein binding; NF-kappaB binding; protein binding; enzyme binding; ubiquitin protein ligase binding; heat shock protein binding; nuclear localization sequence binding; protein complex binding; transcription factor binding
Biological Process: viral reproduction; nerve growth factor receptor signaling pathway; apoptosis; positive regulation of cellular protein metabolic process; toll-like receptor 3 signaling pathway; T cell receptor signaling pathway; toll-like receptor 10 signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 5 signaling pathway; response to exogenous dsRNA; cytoplasmic sequestering of NF-kappaB; positive regulation of interferon type I production; lipopolysaccharide-mediated signaling pathway; toll-like receptor 4 signaling pathway; cytoplasmic sequestering of transcription factor; negative regulation of myeloid cell differentiation; MyD88-independent toll-like receptor signaling pathway; regulation of NF-kappaB import into nucleus; negative regulation of DNA binding; regulation of cell proliferation; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; protein import into nucleus, translocation; response to muramyl dipeptide; inhibition of NF-kappaB transcription factor; toll-like receptor signaling pathway; negative regulation of Notch signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; negative regulation of apoptosis
Reference #:  P25963 (UniProtKB)
Alt. Names/Synonyms: I-kappa-B-alpha; IkappaBalpha; IkB-alpha; IKBA; MAD-3; MAD3; Major histocompatibility complex enhancer-binding protein MAD3; NF-kappa-B inhibitor alpha; NFKBI; NFKBIA; nuclear factor of kappa light chain gene enhancer in B-cells; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
Gene Symbols: NFKBIA
Molecular weight: 35,609 Da
Basal Isoelectric point: 4.57  Predict pI for various phosphorylation states
CST Pathways:  Apoptosis Regulation  |  B Cell Receptor Signaling  |  Death Receptor Signaling  |  Growth And Differentiation Control by MAPKs  |  Inhibition of Apoptosis  |  NF-kB Signaling  |  T Cell Receptor Signaling  |  Toll-Like Receptor Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

IkB-alpha

Protein Structure Not Found.


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Sites Implicated In
apoptosis, altered: S32‑p, S36‑p
apoptosis, induced: S32‑p, S36‑p
apoptosis, inhibited: S32‑p, S36‑p, Y42‑p
transcription, altered: S32‑p, S36‑p, Y42‑p
transcription, induced: S32‑p, S36‑p, Y42‑p
activity, induced: S32‑p, S36‑p, Y42‑p
intracellular localization: S32‑p, S36‑p
molecular association, regulation: Y42‑p
phosphorylation: S32‑p, S36‑p
protein degradation: S32‑p, S36‑p, Y42‑p, S283‑p, T291‑p, S293‑p, T299‑p, Y305‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
4 0 K21-ub EGPRDGLkkERLLDD
4 0 K21-sm EGPRDGLkkERLLDD
4 0 K22-ub GPRDGLkkERLLDDR
1 0 K22-sm GPRDGLkkERLLDDR
120 18 S32-p LLDDRHDsGLDsMkD
81 7 S36-p RHDsGLDsMkDEEyE
1 0 K38-ub DsGLDsMkDEEyEQM
14 27 Y42-p DsMkDEEyEQMVkEL
1 0 K47-ub EEyEQMVkELQEIRL
0 4 K67-ub PRGSEPWkQQLTEDG
0 1 K87-ub LAIIHEEkALtMEVI
0 1 T90-p IHEEkALtMEVIRQV
0 2 K98-ub MEVIRQVkGDLAFLN
0 1 S166-p SVGVLTQsCTTPHLH
0 8 K238-ub DLVSLLLkCGADVNR
0 1 Y248 ADVNRVTYQGYSPYQ
0 1 Y251 NRVTYQGYSPYQLTW
0 1 S252 RVTYQGYSPYQLTWG
3 1 S283-p NLQMLPEsEDEEsYD
1 0 S288-p PEsEDEEsYDtEsEF
4 0 T291-p EDEEsYDtEsEFTEF
3 0 S293-p EEsYDtEsEFTEFtE
2 0 T299-p EsEFTEFtEDELPyD
2 5 Y305-p FtEDELPyDDCVFGG
2859 : Phospho-IkappaB-alpha (Ser32) (14D4) Rabbit mAb
4088 : Phospho-IkB alpha (Ser32/36) (5A5) Mouse mAb (Sepharose Bead Conjugate)
5209 : Phospho-IkB alpha (Ser32) (14D4) Rabbit mAb (Biotinylated)
5210 : Phospho-IkappaB-alpha (Ser32/36) (12C2) Mouse mAb
9241 : Phospho-IkappaB-alpha (Ser32) Antibody
9246 : Phospho-IkappaB-alpha (Ser32/36) (5A5) Mouse mAb
4088 : Phospho-IkB alpha (Ser32/36) (5A5) Mouse mAb (Sepharose Bead Conjugate)
5210 : Phospho-IkappaB-alpha (Ser32/36) (12C2) Mouse mAb
9246 : Phospho-IkappaB-alpha (Ser32/36) (5A5) Mouse mAb
  mouse

 
K21-ub EGPRDGLkkERLVDD
K21-sm EGPRDGLkkERLVDD
K22-ub GPRDGLkkERLVDDR
K22 GPRDGLkKERLVDDR
S32-p LVDDRHDsGLDsMKD
S36-p RHDsGLDsMKDEEYE
K38 DsGLDsMKDEEYEQM
Y42 DsMKDEEYEQMVKEL
K47 EEYEQMVKELREIRL
K67 PLAAEPWKQQLTEDG
K87 LAIIHEEKPLTMEVI
T90 IHEEKPLTMEVIGQV
K98 MEVIGQVKGDLAFLN
T166 SVAVLTQTCTPQHLH
K238 DLVSLLLKCGADVNR
Y248-p ADVNRVTyQGysPYQ
Y251-p NRVTyQGysPYQLTW
S252-p RVTyQGysPYQLTWG
S283 NLQMLPESEDEESYD
S288 PESEDEESYDTEsEF
T291 EDEESYDTEsEFTED
S293-p EESYDTEsEFTEDEL
T296 YDTEsEFTEDELPYD
Y302 FTEDELPYDDCVFGG
2859 : Phospho-IkappaB-alpha (Ser32) (14D4) Rabbit mAb
4088 : Phospho-IkB alpha (Ser32/36) (5A5) Mouse mAb (Sepharose Bead Conjugate)
5209 : Phospho-IkB alpha (Ser32) (14D4) Rabbit mAb (Biotinylated)
5210 : Phospho-IkappaB-alpha (Ser32/36) (12C2) Mouse mAb
9241 : Phospho-IkappaB-alpha (Ser32) Antibody
9246 : Phospho-IkappaB-alpha (Ser32/36) (5A5) Mouse mAb
4088 : Phospho-IkB alpha (Ser32/36) (5A5) Mouse mAb (Sepharose Bead Conjugate)
5210 : Phospho-IkappaB-alpha (Ser32/36) (12C2) Mouse mAb
9246 : Phospho-IkappaB-alpha (Ser32/36) (5A5) Mouse mAb
  rat

 
K21 EGPRDGLKKERLVDD
K21 EGPRDGLKKERLVDD
K22 GPRDGLKKERLVDDR
K22 GPRDGLKKERLVDDR
S32-p LVDDRHDsGLDsMKD
S36-p RHDsGLDsMKDEDyE
K38 DsGLDsMKDEDyEQM
Y42-p DsMKDEDyEQMVKEL
K47 EDyEQMVKELREIRL
K67 PLAAEPWKQQLTEDG
K87 LAIIHEEKTLTMEVI
T90 IHEEKTLTMEVIGQV
K98 MEVIGQVKGDLAFLN
T166 SVAVLTQTCTPQHLH
K238 DLVSLLLKCGADVNR
Y248 ADVNRVTYQGYSPYQ
Y251 NRVTYQGYSPYQLTW
S252 RVTYQGYSPYQLTWG
S283 NLQTLPESEDEESYD
S288 PESEDEESYDTESEF
T291 EDEESYDTESEFTED
S293 EESYDTESEFTEDEL
T296 YDTESEFTEDELPYD
Y302 FTEDELPYDDCVFGG
2859 : Phospho-IkappaB-alpha (Ser32) (14D4) Rabbit mAb
4088 : Phospho-IkB alpha (Ser32/36) (5A5) Mouse mAb (Sepharose Bead Conjugate)
5209 : Phospho-IkB alpha (Ser32) (14D4) Rabbit mAb (Biotinylated)
5210 : Phospho-IkappaB-alpha (Ser32/36) (12C2) Mouse mAb
9241 : Phospho-IkappaB-alpha (Ser32) Antibody
9246 : Phospho-IkappaB-alpha (Ser32/36) (5A5) Mouse mAb
4088 : Phospho-IkB alpha (Ser32/36) (5A5) Mouse mAb (Sepharose Bead Conjugate)
5210 : Phospho-IkappaB-alpha (Ser32/36) (12C2) Mouse mAb
9246 : Phospho-IkappaB-alpha (Ser32/36) (5A5) Mouse mAb
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