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Protein Page:
HSP27 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
HSP27 a small heat shock protein that is regulated both transcriptionally and posttranslationally. Modulates actin polymerization and reorganization. Its expression level increases several-fold in response to stress and is phosphorylated by MAPKAP kinase 2. Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock. Note: This description may include information from UniProtKB.
Protein type: Motility/polarity/chemotaxis; Heat shock protein; Chaperone
Chromosomal Location of Human Ortholog: 7q11.23
Cellular Component: proteasome complex; extracellular space; cytoskeleton; cytoplasm; plasma membrane; spindle; cytosol; Z disc; nucleus
Molecular Function: protein kinase C inhibitor activity; identical protein binding; ubiquitin binding; protein binding; protein kinase C binding; protein kinase binding
Biological Process: cell death; positive regulation of tumor necrosis factor biosynthetic process; response to virus; positive regulation of blood vessel endothelial cell migration; response to unfolded protein; mRNA metabolic process; positive regulation of interleukin-1 beta production; retinal homeostasis; positive regulation of angiogenesis; RNA metabolic process; negative regulation of protein kinase activity; gene expression; cell motility; regulation of I-kappaB kinase/NF-kappaB cascade; regulation of translational initiation; negative regulation of apoptosis
Reference #:  P04792 (UniProtKB)
Alt. Names/Synonyms: 28 kDa heat shock protein; CMT2F; DKFZp586P1322; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; heat shock 27kD protein 1; heat shock 27kDa protein 1; Heat shock protein beta-1; HMN2B; HS.76067; HSP 27; Hsp25; HSP27; HSP28; HSPB1; SRP27; Stress-responsive protein 27
Gene Symbols: HSPB1
Molecular weight: 22,783 Da
Basal Isoelectric point: 5.98  Predict pI for various phosphorylation states
CST Pathways:  Inhibition of Apoptosis  |  Regulation of P38 MAPKs
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

HSP27

Protein Structure Not Found.


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Sites Implicated In
apoptosis, altered: S15‑p, S78‑p, S82‑p
apoptosis, inhibited: S15‑p, S78‑p, S82‑p
cell cycle regulation: S78‑p
cell growth, altered: S15‑p, S78‑p, S82‑p
cytoskeletal reorganization: S15‑p, S78‑p, S82‑p
transcription, altered: S15‑p, S78‑p, S82‑p
activity, induced: S78‑p, S82‑p
intracellular localization: S78‑p, S82‑p
molecular association, regulation: S15‑p, S78‑p, S82‑p
phosphorylation: S15‑p, S78‑p, S82‑p
protein conformation: S15‑p, S78‑p, S82‑p
protein degradation: S15‑p, S78‑p, S82‑p
sumoylation: S15‑p, S78‑p, S82‑p
ubiquitination: S78‑p, S82‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 R5-m1 ___MTERrVPFsLLr
1 5 S9-p TERrVPFsLLrGPsW
0 4 R12-m1 rVPFsLLrGPsWDPF
0 4 - gap
35 76 S15-p FsLLrGPsWDPFRDW
0 8 Y23-p WDPFRDWyPHsRLFD
0 2 S26-p FRDWyPHsRLFDQAF
0 1 R37 DQAFGLPRLPEEWSQ
0 4 Y54-p GGSSWPGyVrPLPPA
0 1 R56-m1 SSWPGyVrPLPPAAI
0 16 S65-p LPPAAIEsPAVAAPA
0 1 - gap
0 13 Y73-p PAVAAPAysRALsRQ
1 5 S74-p AVAAPAysRALsRQL
24 41 S78-p PAysRALsRQLssGV
50 350 S82-p RALsRQLssGVsEIR
1 28 S83-p ALsRQLssGVsEIRH
1 8 S86-p RQLssGVsEIRHtAD
0 1 T91-p GVsEIRHtADRWRVs
1 5 S98-p tADRWRVsLDVNHFA
0 35 K112-ub APDELTVktkDGVVE
1 2 T113-p PDELTVktkDGVVEI
0 45 K114-ub DELTVktkDGVVEIt
0 2 T121-p kDGVVEItGkHEERQ
0 8 K123-ac GVVEItGkHEERQDE
0 14 K123-ub GVVEItGkHEERQDE
0 25 Y133-p ERQDEHGyIsRCFtR
0 6 S135-p QDEHGyIsRCFtRKy
0 5 T139-p GyIsRCFtRKytLPP
0 1 K141 IsRCFtRKytLPPGV
0 3 Y142-p sRCFtRKytLPPGVD
2 13 T143-p RCFtRKytLPPGVDP
0 1 T162-p SSLSPEGtLtVEAPM
0 1 T164-p LSPEGtLtVEAPMPK
0 8 T174-p APMPKLAtQsNEItI
1 2 S176-p MPKLAtQsNEItIPV
0 1 T180-p AtQsNEItIPVtFEs
0 2 T184-p NEItIPVtFEsRAQL
0 2 S187-p tIPVtFEsRAQLGGP
0 51 K198-ub LGGPEAAksDEtAAk
0 11 S199-p GGPEAAksDEtAAk_
0 1 T202-p EAAksDEtAAk____
0 53 K205-ac ksDEtAAk_______
0 3 K205-ub ksDEtAAk_______
2404 : Phospho-HSP27 (Ser15) Antibody
2405 : Phospho-HSP27 (Ser78) Antibody
2401 : Phospho-HSP27 (Ser82) Antibody
2406 : Phospho-HSP27 (Ser82) Antibody II
9709 : Phospho-HSP27 (Ser82) (D1H2) XP(R) Rabbit mAb
11892 : Phospho-HSP27 (Ser82) (D1H2) XP(R) Rabbit mAb (Alexa Fluor(R) 647 Conjugate)
12172 : Phospho-HSP27 (Ser82) (D1H2) XP(R) Rabbit mAb (Alexa Fluor(R) 488 Conjugate)
  mouse

 
R5 ___MTERRVPFSLLr
S9 TERRVPFSLLrsPsW
R12-m1 RVPFSLLrsPsWEPF
S13-p VPFSLLrsPsWEPFR
S15-p FSLLrsPsWEPFRDW
Y23 WEPFRDWYPAHSRLF
S27 RDWYPAHSRLFDQAF
R38 DQAFGVPRLPDEWSQ
Y55 SAAGWPGYVRPLPAA
R57 AGWPGYVRPLPAATA
T63 VRPLPAATAEGPAAV
T71-p AEGPAAVtLAAPAFs
- gap
S78-p tLAAPAFsRALNRQL
N82 PAFsRALNRQLssGV
S86-p RALNRQLssGVsEIR
S87-p ALNRQLssGVsEIRQ
S90-p RQLssGVsEIRQTAD
T95 GVsEIRQTADRWRVS
S102 TADRWRVSLDVNHFA
K116-ub APEELTVkTkEGVVE
T117 PEELTVkTkEGVVEI
K118-ub EELTVkTkEGVVEIT
T125 kEGVVEITGKHEERQ
K127 GVVEITGKHEERQDE
K127 GVVEITGKHEERQDE
Y137 ERQDEHGYIsRCFTR
S139-p QDEHGYIsRCFTRkY
T143 GYIsRCFTRkYtLPP
K145-ub IsRCFTRkYtLPPGV
Y146 sRCFTRkYtLPPGVD
T147-p RCFTRkYtLPPGVDP
T166 SSLSPEGTLTVEAPL
T168 LSPEGTLTVEAPLPK
T178 APLPKAVTQSAEITI
S180 LPKAVTQSAEITIPV
T184 VTQSAEITIPVTFEA
T188 AEITIPVTFEARAQI
- gap
K202-ub IGGPEAGkSEQSGAK
S203 GGPEAGkSEQSGAK_
S206 EAGkSEQSGAK____
K209 kSEQSGAK_______
K209 kSEQSGAK_______
2401 : Phospho-HSP27 (Ser82) Antibody
2406 : Phospho-HSP27 (Ser82) Antibody II
9709 : Phospho-HSP27 (Ser82) (D1H2) XP(R) Rabbit mAb
11892 : Phospho-HSP27 (Ser82) (D1H2) XP(R) Rabbit mAb (Alexa Fluor(R) 647 Conjugate)
12172 : Phospho-HSP27 (Ser82) (D1H2) XP(R) Rabbit mAb (Alexa Fluor(R) 488 Conjugate)
  rat

 
R5 ___MTERRVPFsLLR
S9-p TERRVPFsLLRsPsW
R12 RVPFsLLRsPsWEPF
S13-p VPFsLLRsPsWEPFR
S15-p FsLLRsPsWEPFRDW
Y23 WEPFRDWYPAHsRLF
S27-p RDWYPAHsRLFDQAF
R38-m1 DQAFGVPrFPDEWSQ
Y55 SSAGWPGYVRPLPAA
R57 AGWPGYVRPLPAATA
T63 VRPLPAATAEGPAAV
- gap
- gap
S78 TLARPAFSRALNRQL
N82 PAFSRALNRQLssGV
S86-p RALNRQLssGVsEIR
S87-p ALNRQLssGVsEIRQ
S90-p RQLssGVsEIRQTAD
T95 GVsEIRQTADRWRVs
S102-p TADRWRVsLDVNHFA
K116 APEELTVKtKEGVVE
T117-p PEELTVKtKEGVVEI
K118 EELTVKtKEGVVEIT
T125 KEGVVEITGkHEERQ
K127-ac GVVEITGkHEERQDE
K127 GVVEITGKHEERQDE
Y137 ERQDEHGYISRCFTR
S139 QDEHGYISRCFTRKY
T143 GYISRCFTRKYtLPP
K145 ISRCFTRKYtLPPGV
Y146 SRCFTRKYtLPPGVD
T147-p RCFTRKYtLPPGVDP
T166 SSLSPEGTLTVEAPL
T168 LSPEGTLTVEAPLPK
T178 APLPKAVTQSAEITI
S180 LPKAVTQSAEITIPV
T184 VTQSAEITIPVTFEA
T188 AEITIPVTFEARAQI
- gap
- gap
S200 AQIGGPESEQSGAK_
S203 GGPESEQSGAK____
K206 ESEQSGAK_______
K206 ESEQSGAK_______
2401 : Phospho-HSP27 (Ser82) Antibody
2406 : Phospho-HSP27 (Ser82) Antibody II
9709 : Phospho-HSP27 (Ser82) (D1H2) XP(R) Rabbit mAb
11892 : Phospho-HSP27 (Ser82) (D1H2) XP(R) Rabbit mAb (Alexa Fluor(R) 647 Conjugate)
12172 : Phospho-HSP27 (Ser82) (D1H2) XP(R) Rabbit mAb (Alexa Fluor(R) 488 Conjugate)
  pig

 
R5 ___MTERRVPFSLLR
S9 TERRVPFSLLRSPSW
R12 RVPFSLLRSPSWDPF
S13 VPFSLLRSPSWDPFR
S15 FSLLRSPSWDPFRDW
Y23 WDPFRDWYPAHSRLF
S27 RDWYPAHSRLFDQAF
R38 DQAFGLPRLPEEWSQ
Y55 SHSGWPGYVRPLPPP
R57 SGWPGYVRPLPPPAI
- gap
- gap
Y75 AAVAAPAYSRLLSRQ
S76 AVAAPAYSRLLSRQL
S80 PAYSRLLSRQLsSGV
S84-p RLLSRQLsSGVSEIQ
S85 LLSRQLsSGVSEIQQ
S88 RQLsSGVSEIQQTAD
T93 GVSEIQQTADRWRVS
S100 TADRWRVSLDVNHFA
K114 APEELTVKTKDGVVE
T115 PEELTVKTKDGVVEI
K116 EELTVKTKDGVVEIT
T123 KDGVVEITGKHEERQ
K125 GVVEITGKHEERQDE
K125 GVVEITGKHEERQDE
F135 ERQDEHGFISRCFTR
S137 QDEHGFISRCFTRKY
T141 GFISRCFTRKYTLPP
K143 ISRCFTRKYTLPPGV
Y144 SRCFTRKYTLPPGVD
T145 RCFTRKYTLPPGVDP
T164 SSLSPEGTLSVEAPL
S166 LSPEGTLSVEAPLPK
T176 APLPKPATQSAEITI
S178 LPKPATQSAEITIPV
T182 ATQSAEITIPVTFEA
T186 AEITIPVTFEARAQL
- gap
K200 LGGTEAGKSEKPGTK
S201 GGTEAGKSEKPGTK_
- gap
K207 KSEKPGTK_______
K207 KSEKPGTK_______
  hamster

 
R5 ___MTERRVPFSLLR
S9 TERRVPFSLLRSPsW
R12 RVPFSLLRSPsWEPF
S13 VPFSLLRSPsWEPFR
S15-p FSLLRSPsWEPFRDW
Y23 WEPFRDWYPAHSRLF
S27 RDWYPAHSRLFDQAF
R38 DQAFGVPRLPDEWSQ
Y55 SAAGWPGYVRPLPAA
R57 AGWPGYVRPLPAATA
T63 VRPLPAATAEGPAAV
- gap
- gap
H82 PLAAPAFHRALNRQL
N86 PAFHRALNRQLsSGV
S90-p RALNRQLsSGVSEIR
S91 ALNRQLsSGVSEIRQ
S94 RQLsSGVSEIRQTAD
T99 GVSEIRQTADRWRVS
S106 TADRWRVSLDVNHFA
K120 APEELTVKTKEGVVE
T121 PEELTVKTKEGVVEI
K122 EELTVKTKEGVVEIT
T129 KEGVVEITGKHEERQ
K131 GVVEITGKHEERQDE
K131 GVVEITGKHEERQDE
Y141 ERQDEHGYISRCFTR
S143 QDEHGYISRCFTRKY
T147 GYISRCFTRKYTLPP
K149 ISRCFTRKYTLPPGV
Y150 SRCFTRKYTLPPGVD
T151 RCFTRKYTLPPGVDP
T170 SSLSPEGTLTVEAPL
T172 LSPEGTLTVEAPLPK
T182 APLPKTATQSAEITI
S184 LPKTATQSAEITIPV
T188 ATQSAEITIPVTFEA
T192 AEITIPVTFEARAQI
- gap
K206 IGGQEAGKSEQSGAK
S207 GGQEAGKSEQSGAK_
S210 EAGKSEQSGAK____
K213 KSEQSGAK_______
K213 KSEQSGAK_______
9709 : Phospho-HSP27 (Ser82) (D1H2) XP(R) Rabbit mAb
11892 : Phospho-HSP27 (Ser82) (D1H2) XP(R) Rabbit mAb (Alexa Fluor(R) 647 Conjugate)
12172 : Phospho-HSP27 (Ser82) (D1H2) XP(R) Rabbit mAb (Alexa Fluor(R) 488 Conjugate)
  chicken

 
R5 ___MAERRVPFTFLT
T9 AERRVPFTFLTSPsW
T12 RVPFTFLTSPsWEPF
S13 VPFTFLTSPsWEPFR
S15-p FTFLTSPsWEPFRDW
Y23 WEPFRDWYHGSRLFD
S26 FRDWYHGSRLFDQSF
H37 DQSFGMPHIPEDWYK
Y54 SGSAWPGYFRLLPSE
R56 SAWPGYFRLLPSESA
S62 FRLLPSESALLPAPG
- gap
Y72 LPAPGSPYGRALsEL
G73 PAPGSPYGRALsELS
S77-p SPYGRALsELSsGIS
S80 GRALsELSsGISEIR
S81-p RALsELSsGISEIRQ
S84 sELSsGISEIRQSAD
S89 GISEIRQSADSWKVT
T96 SADSWKVTLDVNHFA
K110 APEELVVKTKDNIVE
T111 PEELVVKTKDNIVEI
K112 EELVVKTKDNIVEIT
T119 KDNIVEITGKHEEKQ
K121 NIVEITGKHEEKQDE
K121 NIVEITGKHEEKQDE
F131 EKQDEHGFISRCFTR
S133 QDEHGFISRCFTRKY
T137 GFISRCFTRKYTLPP
K139 ISRCFTRKYTLPPGV
Y140 SRCFTRKYTLPPGVE
T141 RCFTRKYTLPPGVEA
M160 SSLSPDGMLTVEAPL
T162 LSPDGMLTVEAPLPK
I172 APLPKPAIQSSEITI
S174 LPKPAIQSSEITIPV
T178 AIQSSEITIPVTVEA
T182 SEITIPVTVEAKKEE
- gap
K186 IPVTVEAKKEEPAKK
K187 PVTVEAKKEEPAKK_
- gap
K193 KKEEPAKK_______
K193 KKEEPAKK_______
  dog

 
R5 ___MTERRVPFSLLR
S9 TERRVPFSLLRSPsW
R12 RVPFSLLRSPsWDPF
S13 VPFSLLRSPsWDPFR
S15-p FSLLRSPsWDPFRDW
Y23 WDPFRDWYPAHSRLF
S27 RDWYPAHSRLFDQAF
R38 DQAFGLPRLPEEWAQ
Y55 GHSGWPGYVRPIPPA
R57 SGWPGYVRPIPPAVE
- gap
- gap
Y77 AAAAAPAYsRALSRQ
S78-p AAAAPAYsRALSRQL
S82 PAYsRALSRQLsSGV
S86-p RALSRQLsSGVSEIR
S87 ALSRQLsSGVSEIRQ
S90 RQLsSGVSEIRQTAD
T95 GVSEIRQTADRWRVS
S102 TADRWRVSLDVNHFA
K116 APEELTVKTKDGVVE
T117 PEELTVKTKDGVVEI
K118 EELTVKTKDGVVEIT
T125 KDGVVEITGKHEERQ
K127 GVVEITGKHEERQDE
K127 GVVEITGKHEERQDE
Y137 ERQDEHGYISRRLTP
S139 QDEHGYISRRLTPKY
T143 GYISRRLTPKYTLPP
K145 ISRRLTPKYTLPPGV
Y146 SRRLTPKYTLPPGVD
T147 RRLTPKYTLPPGVDP
T166 SSLSPEGTLTVEAPM
T168 LSPEGTLTVEAPMPK
T178 APMPKPATQSAEITI
S180 MPKPATQSAEITIPV
T184 ATQSAEITIPVTFEA
T188 AEITIPVTFEARAQI
- gap
K202 IGGPEAGKSEQSGAK
S203 GGPEAGKSEQSGAK_
S206 EAGKSEQSGAK____
K209 KSEQSGAK_______
K209 KSEQSGAK_______
9709 : Phospho-HSP27 (Ser82) (D1H2) XP(R) Rabbit mAb
11892 : Phospho-HSP27 (Ser82) (D1H2) XP(R) Rabbit mAb (Alexa Fluor(R) 647 Conjugate)
12172 : Phospho-HSP27 (Ser82) (D1H2) XP(R) Rabbit mAb (Alexa Fluor(R) 488 Conjugate)
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