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Protein Page:
H4 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
H4 histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The gene encoding H4 is intronless. Note: This description may include information from UniProtKB.
Protein type: DNA binding protein
Reference #:  P62805 (UniProtKB)
Alt. Names/Synonyms: H4; H4 histone family, member A; H4/A; H4/B; H4/C; H4/D; H4/E; H4/G; H4/H; H4/I; H4/J; H4/K; H4/M; H4/N; H4/O; H4F2; H4FA; H4FB; H4FC; H4FD; H4FE; H4FG; H4FH; H4FI; H4FJ; H4FK; H4FM; H4FN; H4FO; HIST1H4A; HIST1H4B; HIST1H4C; HIST1H4D; HIST1H4E; HIST1H4F; HIST1H4H; HIST1H4I; HIST1H4J; HIST1H4K; HIST1H4L; HIST2H4; HIST2H4A; HIST2H4B; HIST4H4; histone 1, H4a; histone cluster 1, H4a; Histone H4
Gene Symbols: HIST1H4A
Molecular weight: 11,367 Da
Basal Isoelectric point: 11.36  Predict pI for various phosphorylation states
CST Pathways:  Crosstalk between PTMs  |  Histone Methylation
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

H4

Protein Structure Not Found.


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Sites Implicated In
cell cycle regulation: K21‑m1
molecular association, regulation: K21‑m1

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
1 1 S2-p ______MsGrGkGGk
5 0 R4-me ____MsGrGkGGkGL
3 0 R4-m1 ____MsGrGkGGkGL
6 0 R4-m2 ____MsGrGkGGkGL
49 503 K6-ac __MsGrGkGGkGLGk
59 516 K9-ac sGrGkGGkGLGkGGA
61 522 K13-ac kGGkGLGkGGAkRHR
0 2 K13-m3 kGGkGLGkGGAkRHR
0 2 K13-ub kGGkGLGkGGAkRHR
1 0 K13-sm kGGkGLGkGGAkRHR
0 1 K13-sc kGGkGLGkGGAkRHR
71 523 K17-ac GLGkGGAkRHRkVLR
0 3 K17-m3 GLGkGGAkRHRkVLR
1 0 H19 GkGGAkRHRkVLRDN
0 1 K21 GGAkRHRKVLRDNIQ
3 0 K21-me GGAkRHRkVLRDNIQ
25 8 K21-m1 GGAkRHRkVLRDNIQ
23 6 K21-m2 GGAkRHRkVLRDNIQ
32 2 K21-m3 GGAkRHRkVLRDNIQ
0 1 R24 kRHRkVLRDNIQGIT
0 1 R24 kRHRkVLRDNIQGIT
0 7 K32-ac DNIQGITkPAIRRLA
0 1 K32-m1 DNIQGITkPAIRRLA
1 1 K32-m2 DNIQGITkPAIRRLA
0 129 K32-ub DNIQGITkPAIRRLA
0 2 K32-sc DNIQGITkPAIRRLA
0 1 R36 GITkPAIRRLARRGG
3 36 S48-p RGGVKRIsGLIyEET
0 688 Y52-p KRIsGLIyEETrGVL
1 0 R56-me GLIyEETrGVLkVFL
1 0 R56-m2 GLIyEETrGVLkVFL
0 34 K60-ac EETrGVLkVFLENVI
0 1 K60-m1 EETrGVLkVFLENVI
0 1 K60-m2 EETrGVLkVFLENVI
0 1 K60-m3 EETrGVLkVFLENVI
0 63 K60-ub EETrGVLkVFLENVI
1 145 Y73-p VIRDAVTyTEHAkRk
1 0 H76 DAVTyTEHAkRktVt
1 93 K78-ac VTyTEHAkRktVtAM
1 0 K78-me VTyTEHAkRktVtAM
0 36 K78-ub VTyTEHAkRktVtAM
0 2 K78-sc VTyTEHAkRktVtAM
0 3 K80-ub yTEHAkRktVtAMDV
0 1 K80 yTEHAkRKtVtAMDV
1 8 T81-p TEHAkRktVtAMDVV
0 4 T83-p HAkRktVtAMDVVyA
1 1033 Y89-p VtAMDVVyALkRQGR
2 239 K92-ac MDVVyALkRQGRTLY
1 16 K92-ub MDVVyALkRQGRTLY
0 2 K92-sc MDVVyALkRQGRTLY
8647 : Acetyl-Histone H4 (Lys5) (D12B3) Rabbit mAb
9672 : Acetyl-Histone H4 (Lys5) Antibody
2594 : Acetyl-Histone H4 (Lys8) Antibody
2591 : Acetyl-Histone H4 (Lys12) Antibody
13944 : Acetyl-Histone H4 (Lys12) (D2W6O) Rabbit mAb
8804 : Acetyl-Histone H4 (Lys16) Antibody
13534 : Acetyl-Histone H4 (Lys16) (E2B8W) Rabbit mAb
9724 : Mono-Methyl-Histone H4 (Lys20) Antibody
9759
5737 : Tri-Methyl-Histone H4 (Lys20) (D84D2) Rabbit mAb
  mouse

 
S2 ______MSGrGkGGk
R4 ____MSGRGkGGkGL
R4 ____MSGRGkGGkGL
R4-m2 ____MSGrGkGGkGL
K6-ac __MSGrGkGGkGLGk
K9-ac SGrGkGGkGLGkGGA
K13-ac kGGkGLGkGGAkRHR
K13 kGGkGLGKGGAkRHR
K13 kGGkGLGKGGAkRHR
K13 kGGkGLGKGGAkRHR
K13 kGGkGLGKGGAkRHR
K17-ac GLGkGGAkRHRkVLr
K17-m3 GLGkGGAkRHRkVLr
H19 GkGGAkRHRkVLrDN
K21-ac GGAkRHRkVLrDNIQ
K21 GGAkRHRKVLrDNIQ
K21-m1 GGAkRHRkVLrDNIQ
K21-m2 GGAkRHRkVLrDNIQ
K21-m3 GGAkRHRkVLrDNIQ
R24-m1 kRHRkVLrDNIQGIT
R24-m2 kRHRkVLrDNIQGIT
K32-ac DNIQGITkPAIrRLA
K32 DNIQGITKPAIrRLA
K32 DNIQGITKPAIrRLA
K32-ub DNIQGITkPAIrRLA
K32-sc DNIQGITkPAIrRLA
R36-m1 GITkPAIrRLARRGG
S48-p RGGVKRIsGLIyEET
Y52-p KRIsGLIyEETRGVL
R56 GLIyEETRGVLkVFL
R56 GLIyEETRGVLkVFL
K60 EETRGVLKVFLENVI
K60 EETRGVLKVFLENVI
K60 EETRGVLKVFLENVI
K60 EETRGVLKVFLENVI
K60-ub EETRGVLkVFLENVI
Y73-p VIRDAVTyTEHAkRk
H76 DAVTyTEHAkRktVT
K78-ac VTyTEHAkRktVTAM
K78 VTyTEHAKRktVTAM
K78-ub VTyTEHAkRktVTAM
K78-sc VTyTEHAkRktVTAM
K80 yTEHAkRKtVTAMDV
K80-sc yTEHAkRktVTAMDV
T81-p TEHAkRktVTAMDVV
T83 HAkRktVTAMDVVyA
Y89-p VTAMDVVyALkRQGR
K92-ac MDVVyALkRQGRTLY
K92-ub MDVVyALkRQGRTLY
K92-sc MDVVyALkRQGRTLY
8647 : Acetyl-Histone H4 (Lys5) (D12B3) Rabbit mAb
9672 : Acetyl-Histone H4 (Lys5) Antibody
2594 : Acetyl-Histone H4 (Lys8) Antibody
2591 : Acetyl-Histone H4 (Lys12) Antibody
13944 : Acetyl-Histone H4 (Lys12) (D2W6O) Rabbit mAb
8804 : Acetyl-Histone H4 (Lys16) Antibody
13534 : Acetyl-Histone H4 (Lys16) (E2B8W) Rabbit mAb
9724 : Mono-Methyl-Histone H4 (Lys20) Antibody
9759
5737 : Tri-Methyl-Histone H4 (Lys20) (D84D2) Rabbit mAb
  rat

 
S2 ______MSGRGkGGk
R4 ____MSGRGkGGkGL
R4 ____MSGRGkGGkGL
R4 ____MSGRGkGGkGL
K6-ac __MSGRGkGGkGLGk
K9-ac SGRGkGGkGLGkGGA
K13-ac kGGkGLGkGGAkRHR
K13 kGGkGLGKGGAkRHR
K13 kGGkGLGKGGAkRHR
K13 kGGkGLGKGGAkRHR
K13 kGGkGLGKGGAkRHR
K17-ac GLGkGGAkRHRKVLR
K17 GLGkGGAKRHRKVLR
H19 GkGGAkRHRKVLRDN
K21 GGAkRHRKVLRDNIQ
K21 GGAkRHRKVLRDNIQ
K21 GGAkRHRKVLRDNIQ
K21 GGAkRHRKVLRDNIQ
K21 GGAkRHRKVLRDNIQ
R24 kRHRKVLRDNIQGIT
R24 kRHRKVLRDNIQGIT
K32 DNIQGITKPAIRRLA
K32 DNIQGITKPAIRRLA
K32 DNIQGITKPAIRRLA
K32 DNIQGITKPAIRRLA
K32 DNIQGITKPAIRRLA
R36 GITKPAIRRLARRGG
S48-p RGGVKRIsGLIyEET
Y52-p KRIsGLIyEETRGVL
R56 GLIyEETRGVLKVFL
R56 GLIyEETRGVLKVFL
K60 EETRGVLKVFLENVI
K60 EETRGVLKVFLENVI
K60 EETRGVLKVFLENVI
K60 EETRGVLKVFLENVI
K60 EETRGVLKVFLENVI
Y73 VIRDAVTYTEHAKRK
H76 DAVTYTEHAKRKTVT
K78 VTYTEHAKRKTVTAM
K78 VTYTEHAKRKTVTAM
K78 VTYTEHAKRKTVTAM
K78 VTYTEHAKRKTVTAM
K80 YTEHAKRKTVTAMDV
K80 YTEHAKRKTVTAMDV
T81 TEHAKRKTVTAMDVV
T83 HAKRKTVTAMDVVyA
Y89-p VTAMDVVyALKRQGR
K92 MDVVyALKRQGRTLY
K92 MDVVyALKRQGRTLY
K92 MDVVyALKRQGRTLY
8647 : Acetyl-Histone H4 (Lys5) (D12B3) Rabbit mAb
9672 : Acetyl-Histone H4 (Lys5) Antibody
2591 : Acetyl-Histone H4 (Lys12) Antibody
13944 : Acetyl-Histone H4 (Lys12) (D2W6O) Rabbit mAb
8804 : Acetyl-Histone H4 (Lys16) Antibody
13534 : Acetyl-Histone H4 (Lys16) (E2B8W) Rabbit mAb
5737 : Tri-Methyl-Histone H4 (Lys20) (D84D2) Rabbit mAb
  pig

 
S2 ______MSGRGKGGk
R4 ____MSGRGKGGkGL
R4 ____MSGRGKGGkGL
R4 ____MSGRGKGGkGL
K6 __MSGRGKGGkGLGK
K9-ac SGRGKGGkGLGKGGA
K13 KGGkGLGKGGAKRHR
K13 KGGkGLGKGGAKRHR
K13 KGGkGLGKGGAKRHR
K13 KGGkGLGKGGAKRHR
K13 KGGkGLGKGGAKRHR
K17 GLGKGGAKRHRKVLR
K17 GLGKGGAKRHRKVLR
H19 GKGGAKRHRKVLRDN
K21 GGAKRHRKVLRDNIQ
K21 GGAKRHRKVLRDNIQ
K21 GGAKRHRKVLRDNIQ
K21 GGAKRHRKVLRDNIQ
K21 GGAKRHRKVLRDNIQ
R24 KRHRKVLRDNIQGIT
R24 KRHRKVLRDNIQGIT
K32 DNIQGITKPAIRRLA
K32 DNIQGITKPAIRRLA
K32 DNIQGITKPAIRRLA
K32 DNIQGITKPAIRRLA
K32 DNIQGITKPAIRRLA
R36 GITKPAIRRLARRGG
S48 RGGVKRISGLIYEET
Y52 KRISGLIYEETRGVL
R56 GLIYEETRGVLKVFL
R56 GLIYEETRGVLKVFL
K60 EETRGVLKVFLENVI
K60 EETRGVLKVFLENVI
K60 EETRGVLKVFLENVI
K60 EETRGVLKVFLENVI
K60 EETRGVLKVFLENVI
Y73 VIRDAVTYTEHAKRK
H76 DAVTYTEHAKRKTVT
K78 VTYTEHAKRKTVTAM
K78 VTYTEHAKRKTVTAM
K78 VTYTEHAKRKTVTAM
K78 VTYTEHAKRKTVTAM
K80 YTEHAKRKTVTAMDV
K80 YTEHAKRKTVTAMDV
T81 TEHAKRKTVTAMDVV
T83 HAKRKTVTAMDVVYA
Y89 VTAMDVVYALKRQGR
K92 MDVVYALKRQGRTLY
K92 MDVVYALKRQGRTLY
K92 MDVVYALKRQGRTLY
8647 : Acetyl-Histone H4 (Lys5) (D12B3) Rabbit mAb
9672 : Acetyl-Histone H4 (Lys5) Antibody
2591 : Acetyl-Histone H4 (Lys12) Antibody
13944 : Acetyl-Histone H4 (Lys12) (D2W6O) Rabbit mAb
8804 : Acetyl-Histone H4 (Lys16) Antibody
13534 : Acetyl-Histone H4 (Lys16) (E2B8W) Rabbit mAb
5737 : Tri-Methyl-Histone H4 (Lys20) (D84D2) Rabbit mAb
  cow

 
S2-p ______MsGRGkGGk
R4 ____MsGRGkGGkGL
R4 ____MsGRGkGGkGL
R4 ____MsGRGkGGkGL
K6-ac __MsGRGkGGkGLGk
K9-ac sGRGkGGkGLGkGGA
K13-ac kGGkGLGkGGAkRhR
K13 kGGkGLGKGGAkRhR
K13 kGGkGLGKGGAkRhR
K13 kGGkGLGKGGAkRhR
K13 kGGkGLGKGGAkRhR
K17-ac GLGkGGAkRhRKVLR
K17 GLGkGGAKRhRKVLR
H19-p GkGGAkRhRKVLRDN
K21 GGAkRhRKVLRDNIQ
K21 GGAkRhRKVLRDNIQ
K21 GGAkRhRKVLRDNIQ
K21 GGAkRhRKVLRDNIQ
K21 GGAkRhRKVLRDNIQ
R24 kRhRKVLRDNIQGIT
R24 kRhRKVLRDNIQGIT
K32 DNIQGITKPAIRRLA
K32 DNIQGITKPAIRRLA
K32 DNIQGITKPAIRRLA
K32 DNIQGITKPAIRRLA
K32 DNIQGITKPAIRRLA
R36 GITKPAIRRLARRGG
S48-p RGGVKRIsGLIYEET
Y52 KRIsGLIYEETRGVL
R56 GLIYEETRGVLKVFL
R56 GLIYEETRGVLKVFL
K60 EETRGVLKVFLENVI
K60 EETRGVLKVFLENVI
K60 EETRGVLKVFLENVI
K60 EETRGVLKVFLENVI
K60 EETRGVLKVFLENVI
Y73 VIRDAVTYTEhAKRK
H76-p DAVTYTEhAKRKTVT
K78 VTYTEhAKRKTVTAM
K78 VTYTEhAKRKTVTAM
K78 VTYTEhAKRKTVTAM
K78 VTYTEhAKRKTVTAM
K80 YTEhAKRKTVTAMDV
K80 YTEhAKRKTVTAMDV
T81 TEhAKRKTVTAMDVV
T83 hAKRKTVTAMDVVYA
Y89 VTAMDVVYALKRQGR
K92 MDVVYALKRQGRTLY
K92 MDVVYALKRQGRTLY
K92 MDVVYALKRQGRTLY
8647 : Acetyl-Histone H4 (Lys5) (D12B3) Rabbit mAb
9672 : Acetyl-Histone H4 (Lys5) Antibody
2591 : Acetyl-Histone H4 (Lys12) Antibody
13944 : Acetyl-Histone H4 (Lys12) (D2W6O) Rabbit mAb
8804 : Acetyl-Histone H4 (Lys16) Antibody
13534 : Acetyl-Histone H4 (Lys16) (E2B8W) Rabbit mAb
9724 : Mono-Methyl-Histone H4 (Lys20) Antibody
9759
5737 : Tri-Methyl-Histone H4 (Lys20) (D84D2) Rabbit mAb
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