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Protein Page:
H2B (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
H2B histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The gene encoding H2B is intronless. Note: This description may include information from UniProtKB.
Protein type: DNA binding protein
Cellular Component: nucleoplasm; nucleosome; nucleus
Molecular Function: protein binding; DNA binding; protein heterodimerization activity
Biological Process: nucleosome assembly; establishment and/or maintenance of chromatin architecture
Reference #:  P33778 (UniProtKB)
Alt. Names/Synonyms: H2B histone family, member F; H2B.1; H2B/f; H2B1B; H2BFF; HIST1H2BB; histone 1, H2bb; histone cluster 1, H2bb; Histone H2B type 1-B; Histone H2B.1; Histone H2B.f; MGC119804
Gene Symbols: HIST1H2BB
Molecular weight: 13,950 Da
Basal Isoelectric point: 10.31  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

H2B

Protein Structure Not Found.


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Sites Implicated In
cell growth, induced: S33‑p
translation, induced: S33‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
5 118 K6-ac __MPEPSkSAPAPkk
0 9 K6-ub __MPEPSkSAPAPkk
1 423 K12-ac SkSAPAPkkGskkAI
5 456 K13-ac kSAPAPkkGskkAIT
9 0 S15-p APAPkkGskkAITkA
6 457 K16-ac PAPkkGskkAITkAQ
2 322 K17-ac APkkGskkAITkAQk
3 234 K21-ac GskkAITkAQkkDGK
0 71 K24-ac kAITkAQkkDGKKRK
1 8 K25-ac AITkAQkkDGKKRKR
7 0 S33-p DGKKRKRsRkEsysI
0 4 K35-ub KKRKRsRkEsysIyV
6 42 S37-p RKRsRkEsysIyVyk
0 9 Y38-p KRsRkEsysIyVykV
1 9 S39-p RsRkEsysIyVykVL
0 95 Y41-p RkEsysIyVykVLkQ
0 131 Y43-p EsysIyVykVLkQVH
0 2 K44-ac sysIyVykVLkQVHP
0 4 K44-ub sysIyVykVLkQVHP
1 3 K47-ac IyVykVLkQVHPDTG
1 0 K47-me IyVykVLkQVHPDTG
0 1 K47-m2 IyVykVLkQVHPDTG
0 129 K47-ub IyVykVLkQVHPDTG
0 2 S57-p HPDTGISskAMGIMN
0 4 K58-ac PDTGISskAMGIMNs
1 0 K58-m2 PDTGISskAMGIMNs
0 22 K58-ub PDTGISskAMGIMNs
0 40 S65-p kAMGIMNsFVNDIFE
0 2 G76 DIFERIAGEASRLAH
0 68 Y84-p EASRLAHyNkRstIt
0 17 K86-ac SRLAHyNkRstItsR
0 43 K86-ub SRLAHyNkRstItsR
0 7 S88-p LAHyNkRstItsREI
0 3 T89-p AHyNkRstItsREIQ
0 4 T91-p yNkRstItsREIQTA
0 2 S92-p NkRstItsREIQTAV
0 19 K109-ac LLPGELAkHAVsEGt
1 0 K109-me LLPGELAkHAVsEGt
0 1 K109-m2 LLPGELAkHAVsEGt
0 130 K109-ub LLPGELAkHAVsEGt
0 5 S113-p ELAkHAVsEGtkAVt
1 0 S113-gl ELAkHAVsEGtkAVt
0 2 T116-p kHAVsEGtkAVtkYT
1 6 K117-ac HAVsEGtkAVtkYTS
0 134 K117-ub HAVsEGtkAVtkYTS
1 1 T120-p sEGtkAVtkYTSSk_
1 15 K121-ac EGtkAVtkYTSSk__
8 145 K121-ub EGtkAVtkYTSSk__
0 13 K126-ac VtkYTSSk_______
1 1 K126-ub VtkYTSSk_______
2574 : Acetyl-Histone H2B (Lys5) Antibody
12799 : Acetyl-Histone H2B (Lys5) (D5H1S) XP(R) Rabbit mAb
5410 : Acetyl-Histone H2B (Lys12) Antibody
9072 : Acetyl-Histone H2B (Lys12) (D7H4) Rabbit mAb (ChIP Formulated)
9861 : Acetyl-Histone H2B (Lys12) (D7H4) Rabbit mAb
6959 : Phospho-Histone H2B (Ser14) (D67H2) Rabbit mAb
5435 : Acetyl-Histone H2B (Lys15) Antibody
9083 : Acetyl-Histone H2B (Lys15) (D8H1) XP(R) Rabbit mAb
2571 : Acetyl-Histone H2B (Lys20) Antibody
5546 : Ubiquityl-Histone H2B (Lys120) (D11) XP(R) Rabbit mAb
  mouse

 
K6-ac __MPEPAkSAPAPkk
K6-ub __MPEPAkSAPAPkk
K12-ac AkSAPAPkkGskkAV
K13-ac kSAPAPkkGskkAVT
S15-p APAPkkGskkAVTkA
K16-ac PAPkkGskkAVTkAQ
K17-ac APkkGskkAVTkAQk
K21-ac GskkAVTkAQkkDGK
K24-ac kAVTkAQkkDGKKRK
K25-ac AVTkAQkkDGKKRKR
S33-p DGKKRKRsRkEsysV
K35-ub KKRKRsRkEsysVyV
S37-p RKRsRkEsysVyVyk
Y38-p KRsRkEsysVyVykV
S39-p RsRkEsysVyVykVL
Y41-p RkEsysVyVykVLkQ
Y43-p EsysVyVykVLkQVH
K44-ac sysVyVykVLkQVHP
K44-ub sysVyVykVLkQVHP
K47-ac VyVykVLkQVHPDTG
K47 VyVykVLKQVHPDTG
K47 VyVykVLKQVHPDTG
K47-ub VyVykVLkQVHPDTG
S57-p HPDTGISskAMGIMN
K58 PDTGISsKAMGIMNs
K58 PDTGISsKAMGIMNs
K58-ub PDTGISskAMGIMNs
S65-p kAMGIMNsFVNDIFE
S76-p DIFERIAsEASRLAH
Y84-p EASRLAHyNkRstIT
K86-ac SRLAHyNkRstITsR
K86-ub SRLAHyNkRstITsR
S88-p LAHyNkRstITsREI
T89-p AHyNkRstITsREIQ
T91 yNkRstITsREIQTA
S92-p NkRstITsREIQTAV
K109-ac LLPGELAkHAVsEGt
K109 LLPGELAKHAVsEGt
K109 LLPGELAKHAVsEGt
K109-ub LLPGELAkHAVsEGt
S113-p ELAkHAVsEGtkAVt
S113 ELAkHAVSEGtkAVt
T116-p kHAVsEGtkAVtkYT
K117 HAVsEGtKAVtkYTS
K117-ub HAVsEGtkAVtkYTS
T120-p sEGtkAVtkYTSSk_
K121-ac EGtkAVtkYTSSk__
K121-ub EGtkAVtkYTSSk__
K126-ac VtkYTSSk_______
K126 VtkYTSSK_______
2574 : Acetyl-Histone H2B (Lys5) Antibody
12799 : Acetyl-Histone H2B (Lys5) (D5H1S) XP(R) Rabbit mAb
5410 : Acetyl-Histone H2B (Lys12) Antibody
9072 : Acetyl-Histone H2B (Lys12) (D7H4) Rabbit mAb (ChIP Formulated)
9861 : Acetyl-Histone H2B (Lys12) (D7H4) Rabbit mAb
6959 : Phospho-Histone H2B (Ser14) (D67H2) Rabbit mAb
5435 : Acetyl-Histone H2B (Lys15) Antibody
9083 : Acetyl-Histone H2B (Lys15) (D8H1) XP(R) Rabbit mAb
2571 : Acetyl-Histone H2B (Lys20) Antibody
5546 : Ubiquityl-Histone H2B (Lys120) (D11) XP(R) Rabbit mAb
  rat

 
R6 __MPEPSRSTPAPkk
R6 __MPEPSRSTPAPkk
K12-ac SRSTPAPkkGskkAI
K13-ac RSTPAPkkGskkAIT
S15-p TPAPkkGskkAITkA
K16-ac PAPkkGskkAITkAQ
K17-ac APkkGskkAITkAQK
K21-ac GskkAITkAQKKDGK
K24 kAITkAQKKDGKKRK
K25 AITkAQKKDGKKRKR
G33 DGKKRKRGRKESYSI
K35 KKRKRGRKESYSIYV
S37 RKRGRKESYSIYVYK
Y38 KRGRKESYSIYVYKV
S39 RGRKESYSIYVYKVL
Y41 RKESYSIYVYKVLkQ
Y43 ESYSIYVYKVLkQVH
K44 SYSIYVYKVLkQVHP
K44 SYSIYVYKVLkQVHP
K47 IYVYKVLKQVHPDTG
K47 IYVYKVLKQVHPDTG
K47 IYVYKVLKQVHPDTG
K47-ub IYVYKVLkQVHPDTG
S57 HPDTGISSKAMGIMN
K58 PDTGISSKAMGIMNS
K58 PDTGISSKAMGIMNS
K58 PDTGISSKAMGIMNS
S65 KAMGIMNSFVNDIFE
S76 DIFERIASEASRLAH
Y84 EASRLAHYNKRSTIT
K86 SRLAHYNKRSTITSR
K86 SRLAHYNKRSTITSR
S88 LAHYNKRSTITSREV
T89 AHYNKRSTITSREVQ
T91 YNKRSTITSREVQTA
S92 NKRSTITSREVQTAV
K109 LLPGELAKHAVsEGT
K109 LLPGELAKHAVsEGT
K109 LLPGELAKHAVsEGT
K109-ub LLPGELAkHAVsEGT
S113-p ELAkHAVsEGTkAVT
S113 ELAkHAVSEGTkAVT
T116 kHAVsEGTkAVTkYT
K117 HAVsEGTKAVTkYTS
K117-ub HAVsEGTkAVTkYTS
T120 sEGTkAVTkYTSSK_
K121 EGTkAVTKYTSSK__
K121-ub EGTkAVTkYTSSK__
K126 VTkYTSSK_______
K126 VTkYTSSK_______
2574 : Acetyl-Histone H2B (Lys5) Antibody
12799 : Acetyl-Histone H2B (Lys5) (D5H1S) XP(R) Rabbit mAb
5410 : Acetyl-Histone H2B (Lys12) Antibody
9072 : Acetyl-Histone H2B (Lys12) (D7H4) Rabbit mAb (ChIP Formulated)
9861 : Acetyl-Histone H2B (Lys12) (D7H4) Rabbit mAb
6959 : Phospho-Histone H2B (Ser14) (D67H2) Rabbit mAb
5435 : Acetyl-Histone H2B (Lys15) Antibody
9083 : Acetyl-Histone H2B (Lys15) (D8H1) XP(R) Rabbit mAb
5546 : Ubiquityl-Histone H2B (Lys120) (D11) XP(R) Rabbit mAb
  cow

 
K5 ___PEPAKSAPAPKK
K5 ___PEPAKSAPAPKK
K11 AKSAPAPKKGSKKAV
K12 KSAPAPKKGSKKAVT
S14 APAPKKGSKKAVTKA
K15 PAPKKGSKKAVTKAQ
K16 APKKGSKKAVTKAQK
K20 GSKKAVTKAQKKDGK
K23 KAVTKAQKKDGKKRK
K24 AVTKAQKKDGKKRKR
S32-p DGKKRKRsRKEsYSV
K34 KKRKRsRKEsYSVYV
S36-p RKRsRKEsYSVYVYK
Y37 KRsRKEsYSVYVYKV
S38 RsRKEsYSVYVYKVL
Y40 RKEsYSVYVYKVLKQ
Y42 EsYSVYVYKVLKQVH
K43 sYSVYVYKVLKQVHP
K43 sYSVYVYKVLKQVHP
K46 VYVYKVLKQVHPDTG
K46 VYVYKVLKQVHPDTG
K46 VYVYKVLKQVHPDTG
K46 VYVYKVLKQVHPDTG
S56 HPDTGISSKAMGIMN
K57 PDTGISSKAMGIMNS
K57 PDTGISSKAMGIMNS
K57 PDTGISSKAMGIMNS
S64 KAMGIMNSFVNDIFE
- gap
Y83 EASRLAHYNKRSTIT
K85 SRLAHYNKRSTITSR
K85 SRLAHYNKRSTITSR
S87 LAHYNKRSTITSREI
T88 AHYNKRSTITSREIQ
T90 YNKRSTITSREIQTA
S91 NKRSTITSREIQTAV
K108 LLPGELAKHAVSEGT
K108 LLPGELAKHAVSEGT
K108 LLPGELAKHAVSEGT
K108 LLPGELAKHAVSEGT
S112 ELAKHAVSEGTKAVT
S112 ELAKHAVSEGTKAVT
T115 KHAVSEGTKAVTKYT
K116 HAVSEGTKAVTKYTS
K116 HAVSEGTKAVTKYTS
T119 SEGTKAVTKYTSSK_
K120 EGTKAVTKYTSSK__
K120 EGTKAVTKYTSSK__
K125 VTKYTSSK_______
K125 VTKYTSSK_______
5410 : Acetyl-Histone H2B (Lys12) Antibody
9072 : Acetyl-Histone H2B (Lys12) (D7H4) Rabbit mAb (ChIP Formulated)
9861 : Acetyl-Histone H2B (Lys12) (D7H4) Rabbit mAb
6959 : Phospho-Histone H2B (Ser14) (D67H2) Rabbit mAb
5435 : Acetyl-Histone H2B (Lys15) Antibody
9083 : Acetyl-Histone H2B (Lys15) (D8H1) XP(R) Rabbit mAb
5546 : Ubiquityl-Histone H2B (Lys120) (D11) XP(R) Rabbit mAb
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