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Protein Page:
SLX4 (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
SLX4 regulatory subunit of different structure-specific endonucleases. Required for DNA recombination and repair. Together with SLX1 forms a Holliday junction (HJ) resolvase. Has several distinct roles in protecting genome stability by resolving diverse forms of deleterious DNA structures originating from replication and recombination intermediates and from DNA damage. Component of the SLX1-SLX4 structure-specific endonuclease that resolves DNA secondary structures generated during DNA repair and recombination. Has endonuclease activity towards branched DNA substrates, introducing single-strand cuts in duplex DNA close to junctions with ss-DNA. Has a preference for 5'-flap structures, and promotes symmetrical cleavage of static and migrating HJs. Resolves HJs by generating two pairs of ligatable, nicked duplex products. Interacts with the structure-specific ERCC4-ERCC1 endonuclease and promotes the cleavage of bubble structures. Interacts with the structure-specific MUS81-EME1 endonuclease and promotes the cleavage of 3'-flap and replication fork-like structures. Required for recovery from alkylation-induced DNA damage and is involved in the resolution of DNA double-strand breaks. Involved in Rad1/Rad10pdependent removal of 3'-nonhomologous tails during DSBR via single-strand annealing. Interacts with MSH2; component of the MSH2-MSH3 mismatch repair complex. Interacts with TERF2-TERF2IP. Interacts with PLK1 and C20ORF94. Localizes to sites of DNA damage. Two alternatively spliced human isoforms have been described. Note: This description may include information from UniProtKB.
Protein type: DNA repair, damage; Deoxyribonuclease
Cellular Component: nuclear chromosome, telomeric region; nuclear chromatin
Molecular Function: protein binding; 5'-flap endonuclease activity; 3'-flap endonuclease activity; enzyme activator activity; crossover junction endodeoxyribonuclease activity
Biological Process: positive regulation of catalytic activity; nucleotide-excision repair; DNA replication; DNA repair; DNA catabolic process, endonucleolytic; double-strand break repair via homologous recombination
Reference #:  Q8IY92 (UniProtKB)
Alt. Names/Synonyms: BTB (POZ) domain containing 12; BTB/POZ domain-containing protein 12; BTBD12; KIAA1784; KIAA1987; MUS312; SLX4; Structure-specific endonuclease subunit SLX4
Gene Symbols: SLX4
Molecular weight: 200,012 Da
Basal Isoelectric point: 5.74  Predict pI for various phosphorylation states
Select Structure to View Below

SLX4

Protein Structure Not Found.


STRING  |  Scansite  |  Phospho.ELM  |  NetworKIN  |  Pfam  |  RCSB PDB  |  InnateDB  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene


Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 S169-p TGNQQEPsPNLSREK
0 1 T194 QPPPSCLTTAVPSPS
0 1 T195 PPPSCLTTAVPSPSK
0 1 S199 CLTTAVPSPSKPRTA
0 1 S201 TTAVPSPSKPRTAQL
0 13 S228-p PERLRHAsEECSLEA
0 1 S287-p GASAHDDsLEEKGLF
0 1 S383-p AQPEGSSsPPMFSFS
0 1 K404-m2 KRRGPTSkkEPRKRR
0 1 K405-m2 RRGPTSkkEPRKRRK
0 2 S467-p RKKKPPVsPPLLLVQ
0 2 S499-p SEEVELSsTPPLPAS
0 1 S601-p GCGSRGPsPSASQRE
0 1 S626 AREGLSASPWPGSGG
0 1 K835-u EEAETLLkSKDHEED
0 2 S884-p ADWLEGGsPVSGQLL
0 1 S956-p PEEALGHsSCSSPSR
0 1 - gap
0 8 S998 QGEISEPSQITSEPE
0 1 S1028-p RLAPWQAsPPHPCRF
0 8 S1044-p LGPPQGGsPrGSHHT
0 1 R1046-m1 PPQGGsPrGSHHTSG
0 1 S1066-p PRSRGGTsQVGsPTL
0 5 S1070-p GGTsQVGsPTLLsPA
0 1 L1073 sQVGsPTLLsPAVPS
0 3 S1075-p VGsPTLLsPAVPSKQ
0 2 - gap
0 1 - gap
0 4 - gap
0 8 S1121 VLMFPEKSPsIDLTQ
0 1 S1123-p MFPEKSPsIDLTQSN
0 2 T1127 KSPsIDLTQSNPDHS
0 13 S1135 QSNPDHSSSRsQKSS
1 1 S1138-p PDHSSSRsQKSSSKL
0 2 S1157-p EVILLLDsDEELELE
0 1 K1169-u ELEQTKMkSISSDPL
0 1 K1179-u SSDPLEEkKALEIsP
0 7 S1185-p EkKALEIsPRSCELF
0 2 S1206 ADQEPSQSPPRSEAV
1 3 S1271-p SRSRDCSsQTQISSL
0 6 S1309-p NEVAQKFsVIRPQtP
0 47 T1315-p FsVIRPQtPPPQtPs
0 4 T1320-p PQtPPPQtPssCLTP
0 2 S1322-p tPPPQtPssCLTPVs
0 5 S1323-p PPPQtPssCLTPVsP
0 35 S1329-p ssCLTPVsPGtsDGR
0 7 T1332-p LTPVsPGtsDGRRQG
0 5 S1333-p TPVsPGtsDGRRQGH
0 2 S1342 GRRQGHRSPSRPHPG
0 2 S1344 RQGHRSPSRPHPGGH
0 2 S1452-p RTGPLSTssPsRRMN
0 2 S1453-p TGPLSTssPsRRMNE
0 2 S1455-p PLSTssPsRRMNEAA
0 1 S1464 RMNEAADSRDCRsPG
0 7 S1469-p ADSRDCRsPGLLDTT
0 1 T1475 RsPGLLDTTPIRGSC
0 1 T1476 sPGLLDTTPIRGSCT
0 1 S1608 YTHQTLDSDSEDESQ
0 1 S1610 HQTLDSDSEDESQSS
1 1 S1631-p PHCQTLAsQTYKPSR
0 1 K1657-u GPGAHRPkGPAKTKG
0 1 T1676-p RKHHESItPPSRsPT
0 1 S1681-p SItPPSRsPTKEAPP
1 1 S1716-p GSDSSLSsQSSSSCE
  mouse

 
- gap
T20-p PLPSCFTttGVPsPs
T21-p LPSCFTttGVPsPsK
S25-p FTttGVPsPsKPRVS
S27-p ttGVPsPsKPRVSEL
S54-p PERLRHAsEESPQKT
S111 EASSHHDSLEEKGLF
S207 AQPEVDGSPQVPSFS
K228 KRKGVTTKREPRRRK
R229 RKGVTTKREPRRRKV
- gap
- gap
- gap
S447-p AVEGLSSsPQPGSRG
K649 EEVETLLKPELCEEE
- gap
- gap
S801 LPSNPRVSSELsQIT
S805-p PRVSSELsQITVDHE
T830 ADMAQAPTPHSCSLV
S845 SQSSVDGSPSQSWLH
S847 SSVDGSPSQSWLHLY
S871 SQSHSSISRVAsPRs
S875-p SSISRVAsPRsLsPT
S878-p SRVAsPRsLsPTTPT
S880-p VAsPRsLsPTTPTKQ
S891-p PTKQRRGsNIVTLRK
S909-p HHRGQQSsPIAGHRN
S922-p RNRGILIsPAKsPPI
S926-p ILIsPAKsPPIDLtQ
- gap
T932-p KsPPIDLtQSVPEPL
S940-p QSVPEPLsPRAQDPL
- gap
S962-p EVILLLDsDEELEHT
- gap
K983 KDSPEGRKVPEFsPR
S988-p GRKVPEFsPRSSELF
S1009-p EDHEHFQsPLKREAG
S1073 SRSRDCSSQTQIKSL
S1111 LETAQQFSVIMPHTQ
T1117 FSVIMPHTQPITLGA
- gap
- gap
- gap
T1121 MPHTQPITLGAFDSG
- gap
- gap
S1134-p SGRQAYRsPsHPYPR
S1136-p RQAYRsPsHPYPRHH
S1237 RTGPLTTSsPsSQVL
S1238-p TGPLTTSsPsSQVLE
S1240-p PLTTSsPsSQVLEAL
S1249-p QVLEALHsDDCHsPG
S1254-p LHsDDCHsPGLGttP
T1259-p CHsPGLGttPIRGSC
T1260-p HsPGLGttPIRGSCG
S1382-p YTHQTLEsDsEDEVQ
S1384-p HQTLEsDsEDEVQSP
T1405 PCRQASTTETCNPSR
- gap
- gap
- gap
S1447 GSDNSFSSKSSSAEF
  rat

 
- gap
T28 APNGDSTTTGMPSPS
T29 PNGDSTTTGMPSPSK
S33 STTTGMPSPSKPRAS
S35 TTGMPSPSKPRASEL
S62 PERLRHTSEESPLKT
S121 EASSRPDSLEEKGLF
S217 AQPEAGSSPQVPSFS
K238 KRKGTTNKREPQRRW
R239 RKGTTNKREPQRRWK
- gap
- gap
- gap
S458 AVEGLSSSPCSRSAG
K659 EEAETLLKPEVCEED
S708-p TNQHGEDsPVSEPSL
- gap
S809-p LPSNQRAsLELsQVT
S813-p QRAsLELsQVTVGHE
S838 ANMAQAPSPHPCCLP
S853 SQSSVGDSPSQSWLH
S855 SSVGDSPSQSWLHLY
S877 SVSQSHISKVVSPSS
S881 SHISKVVSPSSPSPV
S884 SKVVSPSSPSPVTPA
S886 VVSPSSPSPVTPAKQ
S897 PAKQRRDSNILTLRK
S915 HHKGRQNSSKLGRRS
S928 RSRSILISPAKSPPI
S932 ILISPAKSPPIDLTQ
- gap
T938 KSPPIDLTQSVPEPL
S946-p QSVPEPLsPRAQDPL
- gap
S968 EVILLLDSDEEWELE
- gap
K990 SKDSPGGKIPEVSPR
S995 GGKIPEVSPRPSDLL
S1016-p GDQEHFPsPPKREAG
S1080 SKSRDRSSQTQIRSL
S1113 LEAAQKFSVIMPHTQ
T1119 FSVIMPHTQPVTVGA
- gap
- gap
- gap
T1123 MPHTQPVTVGASDTR
- gap
S1127 QPVTVGASDTRRQAY
S1136 TRRQAYRSPSRPYLR
S1138 RQAYRSPSRPYLRRH
S1241 RTGPLITSSPGSQVQ
S1242 TGPLITSSPGSQVQE
- gap
S1253 QVQEALQSDGWHSPG
S1258 LQSDGWHSPGLGTTP
T1263 WHSPGLGTTPIRGSC
T1264 HSPGLGTTPIRGSCG
S1395 YTHQTLESDSEDEIQ
S1397 HQTLESDSEDEIQSS
T1418 ARSQASNTETCNPSR
- gap
- gap
- gap
S1460 GSDNSFSSQSSSGEF
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