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Protein Page:
SLX4 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
SLX4 regulatory subunit of different structure-specific endonucleases. Required for DNA recombination and repair. Together with SLX1 forms a Holliday junction (HJ) resolvase. Has several distinct roles in protecting genome stability by resolving diverse forms of deleterious DNA structures originating from replication and recombination intermediates and from DNA damage. Component of the SLX1-SLX4 structure-specific endonuclease that resolves DNA secondary structures generated during DNA repair and recombination. Has endonuclease activity towards branched DNA substrates, introducing single-strand cuts in duplex DNA close to junctions with ss-DNA. Has a preference for 5'-flap structures, and promotes symmetrical cleavage of static and migrating HJs. Resolves HJs by generating two pairs of ligatable, nicked duplex products. Interacts with the structure-specific ERCC4-ERCC1 endonuclease and promotes the cleavage of bubble structures. Interacts with the structure-specific MUS81-EME1 endonuclease and promotes the cleavage of 3'-flap and replication fork-like structures. Required for recovery from alkylation-induced DNA damage and is involved in the resolution of DNA double-strand breaks. Involved in Rad1/Rad10pdependent removal of 3'-nonhomologous tails during DSBR via single-strand annealing. Interacts with MSH2; component of the MSH2-MSH3 mismatch repair complex. Interacts with TERF2-TERF2IP. Interacts with PLK1 and C20ORF94. Localizes to sites of DNA damage. Two alternatively spliced human isoforms have been described. Note: This description may include information from UniProtKB.
Protein type: Deoxyribonuclease; DNA repair, damage
Cellular Component: nuclear chromosome, telomeric region; cytoplasm; nuclear chromatin; nucleolus; cell junction; nucleus
Molecular Function: protein binding; 5'-flap endonuclease activity; 3'-flap endonuclease activity; enzyme activator activity; crossover junction endodeoxyribonuclease activity
Biological Process: positive regulation of catalytic activity; nucleotide-excision repair; DNA repair; DNA replication; DNA catabolic process, endonucleolytic; double-strand break repair via homologous recombination
Reference #:  Q8IY92 (UniProtKB)
Alt. Names/Synonyms: BTB (POZ) domain containing 12; BTB/POZ domain-containing protein 12; BTBD12; KIAA1784; KIAA1987; MUS312; SLX4; Structure-specific endonuclease subunit SLX4
Gene Symbols: SLX4
Molecular weight: 200,012 Da
Basal Isoelectric point: 5.74  Predict pI for various phosphorylation states
Select Structure to View Below

SLX4

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 S57-p DFKELCAsFFQRVKK
0 1 S71-p KHGIKEVsGERKTQK
0 2 S169-p TGNQQEPsPNLsREK
0 1 S173-p QEPsPNLsREKTREN
0 1 T194 QPPPSCLTTAVPSPS
0 1 T195 PPPSCLTTAVPSPSK
0 1 S199 CLTTAVPSPSKPRTA
0 1 S201 TTAVPSPSKPRTAQL
0 15 S228-p PERLRHAsEECSLEA
0 4 S287-p GASAHDDsLEEKGLF
0 1 K341 PECPICGKPFLTLKS
0 2 S383-p AQPEGSSsPPMFSFS
0 1 K404-m2 KRRGPTSkkEPRKRR
0 1 K405-m2 RRGPTSkkEPRKRRK
0 3 S467-p RKKKPPVsPPLLLVQ
0 1 T478-p LLVQDSEtTGRQIED
0 2 S499-p SEEVELSstPPLPAs
0 2 T500-p EEVELSstPPLPAsR
0 1 S506-p stPPLPAsRILKEGW
0 2 T590-p RSPALHGtPTAGCGS
0 1 S601-p GCGSRGPsPSASQRE
0 1 S626 AREGLSASPWPGSGG
0 1 K835-ub EEAETLLkSKDHEED
0 2 S884-p ADWLEGGsPVSGQLL
0 1 S956-p PEEALGHsSCSSPSR
0 1 I994 FSSTQGEISEPSQIT
0 8 S998 QGEISEPSQITSEPE
0 2 S1028-p RLAPWQAsPPHPCRF
0 10 S1044-p LGPPQGGsPrGSHHT
0 1 R1046-m1 PPQGGsPrGSHHTSG
0 1 T1065-p TPRSRGGtsQVGsPt
0 1 S1066-p PRSRGGtsQVGsPtL
0 7 S1070-p GGtsQVGsPtLLsPA
0 1 T1072-p tsQVGsPtLLsPAVP
0 1 L1073 sQVGsPtLLsPAVPS
0 6 S1075-p VGsPtLLsPAVPSKQ
0 3 S1087-p SKQKRDRsILTLsKE
0 1 S1092-p DRsILTLsKEPGHQK
0 1 R1104 HQKGKERRSVLECRN
0 4 F1117 RNKGVLMFPEKSPsI
0 8 S1121 VLMFPEKSPsIDLTQ
0 1 S1123-p MFPEKSPsIDLTQSN
0 2 T1127 KSPsIDLTQSNPDHs
0 1 S1134-p TQSNPDHsSSRsQKS
0 13 S1135 QSNPDHsSSRsQKSS
1 1 S1138-p PDHsSSRsQKSSSKL
0 2 S1157-p EVILLLDsDEELELE
0 1 K1169-ub ELEQTKMksISsDPL
0 1 S1170-p LEQTKMksISsDPLE
0 2 S1173-p TKMksISsDPLEEkK
0 1 K1179-ub SsDPLEEkKALEIsP
0 9 S1185-p EkKALEIsPRSCELF
0 2 S1206 ADQEPSQSPPRSEAV
0 1 T1260-p TSWLVPAtPLAsRSR
0 1 S1264-p VPAtPLAsRSRDCSs
1 4 S1271-p sRSRDCSsQtQISSL
0 1 T1273-p SRDCSsQtQISSLRS
0 1 T1288-p GLAVQAVtQHtPRAS
0 2 T1291-p VQAVtQHtPRASVGN
0 6 S1309-p NEVAQKFsVIRPQtP
0 47 T1315-p FsVIRPQtPPPQtPs
0 4 T1320-p PQtPPPQtPssCLtP
0 2 S1322-p tPPPQtPssCLtPVs
0 5 S1323-p PPPQtPssCLtPVsP
0 1 T1326-p QtPssCLtPVsPGts
0 41 S1329-p ssCLtPVsPGtsDGR
0 7 T1332-p LtPVsPGtsDGRRQG
0 5 S1333-p tPVsPGtsDGRRQGH
0 2 S1342 GRRQGHRSPSRPHPG
0 2 S1344 RQGHRSPSRPHPGGH
0 1 T1446-p DHWNLERtGPLSTss
0 2 S1452-p RtGPLSTssPsRRMN
0 2 S1453-p tGPLSTssPsRRMNE
0 2 S1455-p PLSTssPsRRMNEAA
0 2 S1464-p RMNEAADsRDCRsPG
0 9 S1469-p ADsRDCRsPGLLDTT
0 1 T1475 RsPGLLDTTPIRGSC
0 1 T1476 sPGLLDTTPIRGSCT
0 1 K1551 TPKGANRKKNLPPKV
0 1 K1552 PKGANRKKNLPPKVP
0 1 S1608 YTHQTLDSDSEDESQ
0 1 S1610 HQTLDSDSEDESQSS
1 1 S1631-p PHCQTLAsQTYKPSR
0 1 K1657-ub GPGAHRPkGPAKTKG
0 3 T1676-p RKHHESItPPSRsPt
0 1 S1681-p SItPPSRsPtKEAPP
0 1 T1683-p tPPSRsPtKEAPPGL
1 1 S1716-p GSDSSLSsQSSSSCE
  mouse

 
- under review  
- gap
- gap
- gap
T20-p PLPSCFTttGVPsPs
T21-p LPSCFTttGVPsPsK
S25-p FTttGVPsPsKPRVS
S27-p ttGVPsPsKPRVSEL
S54-p PERLRHAsEESPQKT
S111 EASSHHDSLEEKGLF
K165-ub PDCPICGkPFLTTKS
S207 AQPEVDGSPQVPSFS
K228 KRKGVTTKREPRRRK
R229 RKGVTTKREPRRRKV
C292 RKKRPPVCPPQLVTQ
T303 LVTQDSETTGRQIED
C324 SEEAELSCTPPLLAS
T325 EEAELSCTPPLLASK
S331 CTPPLLASKISKEEL
S411 RPPASQSSLPVSHSP
L422 SHSPKIRLLSSSQRE
S447-p AVEGLSSsPQPGSRG
K649 EEVETLLKPELCEEE
G698 TNPHGEDGAVSEPSL
P767 LGEATDDPSFCSRHR
S801 LPSNPRVSSELsQIT
S805-p PRVSSELsQITVDHE
T830 ADMAQAPTPHSCSLV
S845 SQSSVDGSPSQSWLH
S847 SSVDGSPSQSWLHLY
I870 VSQSHSSISRVAsPR
S871 SQSHSSISRVAsPRs
S875-p SSISRVAsPRsLsPT
R877 ISRVAsPRsLsPTTP
S878-p SRVAsPRsLsPTTPT
S880-p VAsPRsLsPTTPTKQ
S891-p PTKQRRGsNIVTLRK
R897 GsNIVTLRKDAGHHR
S909-p HHRGQQSsPIAGHRN
S922-p RNRGILIsPAKsPPI
S926-p ILIsPAKsPPIDLtQ
P928 IsPAKsPPIDLtQSV
T932-p KsPPIDLtQSVPEPL
L939 tQSVPEPLsPRAQDP
S940-p QSVPEPLsPRAQDPL
A943 PEPLsPRAQDPLHFV
S962-p EVILLLDsDEELEHT
E972 ELEHTKTESVSKDSP
S973 LEHTKTESVSKDSPE
S978 TESVSKDSPEGRKVP
K983 KDSPEGRKVPEFsPR
S988-p GRKVPEFsPRSSELF
S1009-p EDHEHFQsPLKREAG
T1062 TSWLVPATPGVSRSR
S1066 VPATPGVSRSRDCSS
S1073 SRSRDCSSQTQIKSL
T1075 SRDCSSQTQIKSLKT
T1089 TRIPSDETAQQTPRP
T1093 SDETAQQTPRPNLER
S1111 LETAQQFSVIMPHTQ
T1117 FSVIMPHTQPITLGA
- gap
- gap
- gap
- gap
T1121 MPHTQPITLGAFDSG
A1124 TQPITLGAFDSGRQA
F1125 QPITLGAFDSGRQAY
S1134-p SGRQAYRsPsHPYPR
S1136-p RQAYRsPsHPYPRHH
T1231 DHLNLERTGPLTTSs
S1237 RTGPLTTSsPsSQVL
S1238-p TGPLTTSsPsSQVLE
S1240-p PLTTSsPsSQVLEAL
S1249-p QVLEALHsDDCHsPG
S1254-p LHsDDCHsPGLGttP
T1259-p CHsPGLGttPIRGSC
T1260-p HsPGLGttPIRGSCG
K1325-ac TPKGANQkkNLPPKV
K1326-ac PKGANQkkNLPPKVP
S1382-p YTHQTLEsDsEDEVQ
S1384-p HQTLEsDsEDEVQSP
T1405 PCRQASTTETCNPSR
- gap
- gap
- gap
- gap
S1447 GSDNSFSSKSSSAEF
  rat

 
- under review  
- gap
- gap
- gap
T28 APNGDSTTTGMPSPS
T29 PNGDSTTTGMPSPSK
S33 STTTGMPSPSKPRAS
S35 TTGMPSPSKPRASEL
S62 PERLRHTSEESPLKT
S121 EASSRPDSLEEKGLF
K175 PDCPICGKQFLTAKS
S217 AQPEAGSSPQVPSFS
K238 KRKGTTNKREPQRRW
R239 RKGTTNKREPQRRWK
C303 RRKRPPVCPPQLVTQ
T314 LVTQDSETTSRQIED
C335 SEETELACTPPLPAS
T336 EETELACTPPLPASK
S342 CTPPLPASKISKEER
S422 RPPASQSSLPVSHSP
L433 SHSPKSQLLSSSQRE
S458-p AVEGLSSsPCSRSAG
K659 EEAETLLKPEVCEED
S708-p TNQHGEDsPVSEPSL
P771 LEEAVDHPGFCSPSD
S809-p LPSNQRAsLELsQVT
S813-p QRAsLELsQVTVGHE
S838 ANMAQAPSPHPCCLP
S853 SQSSVGDSPSQSWLH
S855 SSVGDSPSQSWLHLY
I876 PSVSQSHISKVVSPS
S877 SVSQSHISKVVSPSS
S881 SHISKVVSPSSPSPV
S883 ISKVVSPSSPSPVTP
S884 SKVVSPSSPSPVTPA
S886 VVSPSSPSPVTPAKQ
S897 PAKQRRDSNILTLRK
R903 DSNILTLRKEAGHHK
S915 HHKGRQNSSKLGRRS
S928 RSRSILISPAKsPPI
S932-p ILISPAKsPPIDLTQ
P934 ISPAKsPPIDLTQSV
T938 KsPPIDLTQSVPEPL
L945 TQSVPEPLsPRAQDP
S946-p QSVPEPLsPRAQDPL
A949 PEPLsPRAQDPLRRV
S968 EVILLLDSDEEWELE
E980 ELEHIKTESVSKDSP
S981 LEHIKTESVSKDSPG
S986 TESVSKDSPGGKIPE
K990 SKDSPGGKIPEVSPR
S995 GGKIPEVSPRPSDLL
S1016-p GDQEHFPsPPKREAG
T1069 SSWLVPATPGASKSR
S1073 VPATPGASKSRDRSS
S1080 SKSRDRSSQTQIRSL
T1082 SRDRSSQTQIRSLKI
T1096 IRTPSGETAQQTPRP
T1100 SGETAQQTPRPPVLE
S1113 LEAAQKFSVIMPHTQ
T1119 FSVIMPHTQPVTVGA
- gap
- gap
- gap
- gap
T1123 MPHTQPVTVGASDTR
A1126 TQPVTVGASDTRRQA
S1127 QPVTVGASDTRRQAY
S1136 TRRQAYRSPSRPYLR
S1138 RQAYRSPSRPYLRRH
T1235 DHLNLERTGPLITSS
S1241 RTGPLITSSPGSQVQ
S1242 TGPLITSSPGSQVQE
G1244 PLITSSPGSQVQEAL
S1253 QVQEALQSDGWHSPG
S1258 LQSDGWHSPGLGTTP
T1263 WHSPGLGTTPIRGSC
T1264 HSPGLGTTPIRGSCG
R1338 TPKGANQRKNLPPKV
K1339 PKGANQRKNLPPKVP
S1395 YTHQTLESDSEDEIQ
S1397 HQTLESDSEDEIQSS
T1418 ARSQASNTETCNPSR
- gap
- gap
- gap
- gap
S1460 GSDNSFSSQSSSGEF
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