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Protein Page:
PHF2 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
PHF2 Lysine demethylase that demethylates both histones and non-histone proteins. Enzymatically inactive by itself, and becomes active following phosphorylation by PKA: forms a complex with ARID5B and mediates demethylation of methylated ARID5B. Demethylation of ARID5B leads to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. PHF2 is recruited to trimethylated 'Lys-4' of histone H3 (H3K4me3) at rDNA promoters and promotes expression of rDNA. Component of the PHF2-ARID5B complex, at least composed of PHF2 and ARID5B. Interacts with HNF4A and NR1H4. Widely expressed, including in liver. Enzymatically inactive by itself, and become active following phosphorylation by PKA. Belongs to the JHDM1 histone demethylase family. JHDM1D subfamily. Note: This description may include information from UniProtKB.
Protein type: EC 1.14.11.-
Cellular Component: cytoplasm; nucleolus; nucleus
Molecular Function: protein binding; histone demethylase activity; dioxygenase activity; zinc ion binding; iron ion binding; transcription coactivator activity; histone demethylase activity (H3-K9 specific); methylated histone residue binding
Biological Process: histone H3-K9 demethylation; transcription, DNA-dependent; liver development; protein amino acid demethylation
Reference #:  O75151 (UniProtKB)
Alt. Names/Synonyms: GRC5; JHDM1E; jumonji C domain-containing histone demethylase 1E; KIAA0662; MGC176680; PHD finger protein 2; PHF2
Gene Symbols: PHF2
Molecular weight: 120,775 Da
Basal Isoelectric point: 9.25  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

PHF2

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 S33 CKDWFHGSCVGVEEE
0 1 Y48 EAPDIDIYHCPNCEK
0 1 T56 HCPNCEKTHGKSTLK
0 1 S86-p VKPVQNGsQLFIKEL
0 1 S147 PAPTFYVSDVENYVG
0 1 K175-ub KDCKMKLkEFVDYYY
0 2 K412-ub AFRSWTKkQALAEHE
0 1 K427-ub DELPEHFkPSQLIKD
0 3 S458-p PEVNTVAsSDEVCDG
0 2 S459 EVNTVAsSDEVCDGD
0 12 S474-p REKEEPPsPIEAtPP
0 1 A478 EPPsPIEAtPPQSLL
0 6 T479-p PPsPIEAtPPQSLLE
0 3 P511 KIPKPPKPPKPPRPP
0 1 S534-p GKKKGKKsREsAsPt
0 6 S537-p KGKKsREsAsPtIPN
0 11 S539-p KKsREsAsPtIPNLD
0 4 T541-p sREsAsPtIPNLDLL
0 1 S570 KKGKATKSVLSVPNK
0 1 S573 KATKSVLSVPNKDVV
0 11 S625-p EEQKLEKsPLAGNkD
0 1 K631-m2 KsPLAGNkDNKFSFS
0 1 S640-p NKFSFSFsNkKLLGS
0 1 K642-m2 FSFSFsNkKLLGSKA
0 5 T654-p SKALRPPtsPGVFGA
1 6 S655-p KALRPPtsPGVFGAL
0 15 S681-p RDEYEYVsDDGELKI
0 9 S705-p NAPKRDLsFLLDKKA
0 1 K720-ac VLPTPVTkPKLDSAA
0 1 Y728 PKLDSAAYKsDDssD
0 5 S730-p LDSAAYKsDDssDEG
0 5 S733-p AAYKsDDssDEGsLH
0 4 S734-p AYKsDDssDEGsLHI
0 3 S738-p DDssDEGsLHIDtDt
0 2 T743-p EGsLHIDtDtKPGRN
0 2 T745-p sLHIDtDtKPGRNAR
1 1 S757-p NARVKKEsGSSAAGI
0 1 S771-p ILDLLQAsEEVGALE
0 4 S853-p LKRAAKNsVDLDDYE
0 7 S879-p DSDYVYPsLEsDEDN
0 21 S882-p YVYPsLEsDEDNPIF
1 13 S899-p RSKKRKGsDDAPYsP
0 6 S905-p GsDDAPYsPTARVGP
0 1 T907 DDAPYsPTARVGPSV
0 1 S913 PTARVGPSVPRQDRP
0 1 T925-p DRPVREGtRVASIET
0 2 S942-p AAAAAKLsQQEEQKS
1 0 S954-p QKSKKKKsAKRKLTP
0 1 S1041 GAQAGRTSQPMAPGV
0 1 T1051 MAPGVFLTQRRPsAs
1 11 S1056-p FLTQRRPsAssPNNN
0 3 S1058-p TQRRPsAssPNNNtA
0 4 S1059-p QRRPsAssPNNNtAA
0 2 T1064-p AssPNNNtAAKGKRT
0 1 K1072-ac AAKGKRTkKGMATAk
0 1 K1079-ac kKGMATAkQRLGKIL
  mouse

 
S33-p CKDWFHGsCVGVEEE
Y48-p EAPDIDIyHCPNCEK
T56-p HCPNCEKtHGKSTLK
S86 VKPVQNGSQLFIKEL
S147 PAPTFYVSDVENYVG
K175 KDCKMKLKEFVDYYY
K412-ub AFRSWTKkQALAEHE
R427 DELPEHFRPSQLIKD
S458-p PEVNAAAssDEVCDG
S459-p EVNAAAssDEVCDGD
S474-p REKEEPPsPVEttPP
T478-p EPPsPVEttPPRSLL
T479-p PPsPVEttPPRSLLE
S511-p KIPKPPKsPKPPKTL
C531 SKKKGKKCKEsAsPt
S534-p KGKKCKEsAsPtIPN
S536-p KKCKEsAsPtIPNLD
T538-p CKEsAsPtIPNLDLL
S567-p KKGKTPKsVLsVPNK
S570-p KTPKsVLsVPNKDTV
S622-p EEQRLEKsPLAGNKD
K628 KsPLAGNKDKFSFSF
S636 DKFSFSFSNRKLLGS
R638 FSFSFSNRKLLGSKA
S650-p SKALRPPssPGVFGA
S651-p KALRPPssPGVFGAL
S677-p RDEYEYVsDDGELKI
S701-p SAPKRDLsFLLDKKE
K716 ALLMPTSKPKLDSAV
Y724-p PKLDSAVyKsDDssD
S726-p LDSAVyKsDDssDEG
S729-p AVyKsDDssDEGsLH
S730-p VyKsDDssDEGsLHI
S734-p DDssDEGsLHIDTDT
T739 EGsLHIDTDTKPGRN
T741 sLHIDTDTKPGRNAK
S753 NAKVKKESGSSAAGI
S767 ILDLLQASEEVGALE
S848-p LKRTAKNsVDLEDYE
S873-p DSDYVYPsLEsDEDN
S876-p YVYPsLEsDEDNPVF
S893-p RSKKRKGsDDAPYsP
S899-p GsDDAPYsPtARVGP
T901-p DDAPYsPtARVGPsV
S907-p PtARVGPsVPRQDRP
T919 DRPVREGTRVASIET
S936 AAAAAKLSQQEEQKN
N948 QKNRKKKNTKRKPAP
S1042-p GSQAGRAsQPMAPGV
T1052-p MAPGVFLtQRRPsAs
S1057-p FLtQRRPsAssPNNt
S1059-p tQRRPsAssPNNtAA
S1060-p QRRPsAssPNNtAAK
T1064-p sAssPNNtAAKGKRT
K1072 AAKGKRTKKGMATAK
K1079 KKGMATAKQRLGKIL
  rat

 
- gap
- gap
- gap
- gap
S30-p PAPTFYVsDVENYVG
K58 KDCKMKLKEFVDYYY
K295 AFRSWTKKQALAEHE
R310 DELPEHFRPSQLIKD
S341 PEVNAVASSDEVCDG
S342 EVNAVASSDEVCDGD
S357-p REKEEPPsPIESTPP
S361 EPPsPIESTPPRSLL
T362 PPsPIESTPPRSLLE
P394 KTPKPPKPPKPPKTL
C414 SKKKGKKCKESAsPT
S417 KGKKCKESAsPTIPN
S419-p KKCKESAsPTIPNLD
T421 CKESAsPTIPNLDLL
S450 KKGKTPKSVLSVPNK
S453 KTPKSVLSVPNKDTV
S505-p EEQRLEKsPLAGNKD
K511 KsPLAGNKDKFSFSF
S519 DKFSFSFSNRKLLGS
R521 FSFSFSNRKLLGSKP
S533 SKPLRPPSSPGVFGT
S534 KPLRPPSSPGVFGTL
S560 RDEYEYVSDDGELKI
S584 NPPKRDLSFLLDKKE
K599 TLLIPTSKPKLDSTA
Y607 PKLDSTAYKSDDSSD
S609 LDSTAYKSDDSSDEG
S612 TAYKSDDSSDEGSLH
S613 AYKSDDSSDEGSLHI
S617 DDSSDEGSLHIDTDT
T622 EGSLHIDTDTKPSRN
T624 SLHIDTDTKPSRNAK
S636 NAKVKKESGSSAAGI
S650 ILDLLQASEEVGALE
S731 LKRTAKNSVDLEDYE
S756 DSDYVYPSLEsDEDN
S759-p YVYPSLEsDEDNPVF
S776 RSKKRKGSDDAPYSP
S782 GSDDAPYSPTARVGP
T784 DDAPYSPTARVGPSV
S790 PTARVGPSVPRQDRP
T802 DRPVREGTRVASIET
S819 AAAAAKLSQQEEQKN
N831 QKNRKKKNTKRKPTP
S925 GAQAGRASQPMAPGV
T935 MAPGVFLTQRRPsAS
S940-p FLTQRRPsASSPNNT
S942 TQRRPsASSPNNTAA
S943 QRRPsASSPNNTAAK
T947 sASSPNNTAAKGKRT
K955 AAKGKRTKKGMATAK
K962 KKGMATAKQRLGKIL
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