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PHF2 (human)

Overview PHF2 Lysine demethylase that demethylates both histones and non-histone proteins. Enzymatically inactive by itself, and becomes active following phosphorylation by PKA: forms a complex with ARID5B and mediates demethylation of methylated ARID5B. Demethylation of ARID5B leads to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. PHF2 is recruited to trimethylated 'Lys-4' of histone H3 (H3K4me3) at rDNA promoters and promotes expression of rDNA. Component of the PHF2-ARID5B complex, at least composed of PHF2 and ARID5B. Interacts with HNF4A and NR1H4. Widely expressed, including in liver. Enzymatically inactive by itself, and become active following phosphorylation by PKA. Belongs to the JHDM1 histone demethylase family. JHDM1D subfamily. Note: This description may include information from UniProtKB. Protein type: EC 1.14.11.- Cellular Component: nucleolus; nucleus Molecular Function: oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; protein binding; histone demethylase activity; zinc ion binding; iron ion binding; transcription coactivator activity; histone demethylase activity (H3-K9 specific); methylated histone residue binding Biological Process: histone H3-K9 demethylation; transcription, DNA-dependent; liver development; protein amino acid demethylation Reference #:  O75151 (UniProtKB) Alt. Names/Synonyms: GRC5; JHDM1E; jumonji C domain-containing histone demethylase 1E; KIAA0662; MGC176680; PHD finger protein 2; PHF2 Gene Symbols: PHF2 Molecular weight: 120,775 Da Basal Isoelectric point: 9.25  Predict pI for various phosphorylation states Protein-Specific Antibodies or siRNAs from Cell Signaling Technology®

Select Structure to View Below PHF2 3KQI - A=1-70 (human) 3PTR - B=60-451 (human) 3PU3 - A/B=60-451 (human) 3PU8 - A/B=60-451 (human) 3PUA - A=60-451 (human) 3PUS - A/B=60-451 (human)

STRING  |  Scansite  |  Phospho.ELM  |  NetworKIN  |  Pfam  |  RCSB PDB 3KQI 3PTR 3PU3 3PU8 3PUA 3PUS  |  Phospho3D  |  DISEASE  |  Source  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene

	Modification Sites and Domains	Show Modification Legend
Click here to view phosphorylation modifications only

	Modification Sites in Parent Protein, Orthologs, and Isoforms	Show Modification Legend

SS  SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.	MS  MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.			human
0	1		S33	CKDWFHGSCVGVEEE
0	1		Y48	EAPDIDIYHCPNCEK
0	1		T56	HCPNCEKTHGKSTLK
0	1		S86-p	VKPVQNGsQLFIKEL
0	1		S147	PAPTFYVSDVENYVG
0	1		K175-u	KDCKMKLkEFVDYYY
0	2		K412-u	AFRSWTKkQALAEHE
0	1		K427-u	DELPEHFkPSQLIKD
0	3		S458-p	PEVNTVAsSDEVCDG
0	2		S459	EVNTVAsSDEVCDGD
0	7		S474-p	REKEEPPsPIEAtPP
0	1		A478	EPPsPIEAtPPQSLL
0	5		T479-p	PPsPIEAtPPQSLLE
0	3		P511	KIPKPPKPPKPPRPP
0	3		S537-p	KGKKSREsAsPtIPN
0	8		S539-p	KKSREsAsPtIPNLD
0	2		T541-p	SREsAsPtIPNLDLL
0	1		S570	KKGKATKSVLSVPNK
0	1		S573	KATKSVLSVPNKDVV
0	9		S625-p	EEQKLEKsPLAGNkD
0	1		K631-m2	KsPLAGNkDNKFSFS
0	1		S640-p	NKFSFSFsNkKLLGS
0	1		K642-m2	FSFSFsNkKLLGSKA
0	4		T654-p	SKALRPPtsPGVFGA
1	4		S655-p	KALRPPtsPGVFGAL
0	14		S681-p	RDEYEYVsDDGELKI
0	6		S705-p	NAPKRDLsFLLDKKA
0	1		Y728	PKLDSAAYKsDDssD
0	4		S730-p	LDSAAYKsDDssDEG
0	4		S733-p	AAYKsDDssDEGsLH
0	4		S734-p	AYKsDDssDEGsLHI
0	3		S738-p	DDssDEGsLHIDtDt
0	1		T743-p	EGsLHIDtDtKPGRN
0	1		T745-p	sLHIDtDtKPGRNAR
1	1		S757-p	NARVKKEsGSSAAGI
0	1		S771-p	ILDLLQAsEEVGALE
0	3		S853-p	LKRAAKNsVDLDDYE
0	5		S879-p	DSDYVYPsLEsDEDN
0	14		S882-p	YVYPsLEsDEDNPIF
1	7		S899-p	RSKKRKGsDDAPYsP
0	3		S905-p	GsDDAPYsPTARVGP
0	1		S913	PTARVGPSVPRQDRP
0	1		T925-p	DRPVREGtRVASIET
0	2		S942-p	AAAAAKLsQQEEQKS
1	0		S954-p	QKSKKKKsAKRKLTP
1	8		S1056-p	FLTQRRPsAssPNNN
0	2		S1058-p	TQRRPsAssPNNNtA
0	2		S1059-p	QRRPsAssPNNNtAA
0	1		T1064-p	AssPNNNtAAKGKRT
0	1		K1072-a	AAKGKRTkKGMATAk
0	1		K1079-a	kKGMATAkQRLGKIL

	mouse
S33-p	CKDWFHGsCVGVEEE
Y48-p	EAPDIDIyHCPNCEK
T56-p	HCPNCEKtHGKSTLK
S86	VKPVQNGSQLFIKEL
S147	PAPTFYVSDVENYVG
K175	KDCKMKLKEFVDYYY
K412-u	AFRSWTKkQALAEHE
R427	DELPEHFRPSQLIKD
S458-p	PEVNAAAssDEVCDG
S459-p	EVNAAAssDEVCDGD
S474-p	REKEEPPsPVEttPP
T478-p	EPPsPVEttPPRSLL
T479-p	PPsPVEttPPRSLLE
S511-p	KIPKPPKsPKPPKTL
S534-p	KGKKCKEsAsPtIPN
S536-p	KKCKEsAsPtIPNLD
T538-p	CKEsAsPtIPNLDLL
S567-p	KKGKTPKsVLsVPNK
S570-p	KTPKsVLsVPNKDTV
S622-p	EEQRLEKsPLAGNKD
K628	KsPLAGNKDKFSFSF
S636	DKFSFSFSNRKLLGS
R638	FSFSFSNRKLLGSKA
S650-p	SKALRPPssPGVFGA
S651-p	KALRPPssPGVFGAL
S677-p	RDEYEYVsDDGELKI
S701-p	SAPKRDLsFLLDKKE
Y724-p	PKLDSAVyKsDDssD
S726-p	LDSAVyKsDDssDEG
S729-p	AVyKsDDssDEGsLH
S730-p	VyKsDDssDEGsLHI
S734-p	DDssDEGsLHIDTDT
T739	EGsLHIDTDTKPGRN
T741	sLHIDTDTKPGRNAK
S753	NAKVKKESGSSAAGI
S767	ILDLLQASEEVGALE
S848-p	LKRTAKNsVDLEDYE
S873-p	DSDYVYPsLEsDEDN
S876-p	YVYPsLEsDEDNPVF
S893-p	RSKKRKGsDDAPYsP
S899-p	GsDDAPYsPTARVGP
S907-p	PTARVGPsVPRQDRP
T919	DRPVREGTRVASIET
S936	AAAAAKLSQQEEQKN
N948	QKNRKKKNTKRKPAP
S1057-p	FLTQRRPsAssPNNT
S1059-p	TQRRPsAssPNNTAA
S1060-p	QRRPsAssPNNTAAK
T1064	sAssPNNTAAKGKRT
K1072	AAKGKRTKKGMATAK
K1079	KKGMATAKQRLGKIL

	rat
-	gap
-	gap
-	gap
-	gap
S30-p	PAPTFYVsDVENYVG
K58	KDCKMKLKEFVDYYY
K295	AFRSWTKKQALAEHE
R310	DELPEHFRPSQLIKD
S341	PEVNAVASSDEVCDG
S342	EVNAVASSDEVCDGD
S357	REKEEPPSPIESTPP
S361	EPPSPIESTPPRSLL
T362	PPSPIESTPPRSLLE
P394	KTPKPPKPPKPPKTL
S417	KGKKCKESASPTIPN
S419	KKCKESASPTIPNLD
T421	CKESASPTIPNLDLL
S450	KKGKTPKSVLSVPNK
S453	KTPKSVLSVPNKDTV
S505	EEQRLEKSPLAGNKD
K511	KSPLAGNKDKFSFSF
S519	DKFSFSFSNRKLLGS
R521	FSFSFSNRKLLGSKP
S533	SKPLRPPSSPGVFGT
S534	KPLRPPSSPGVFGTL
S560	RDEYEYVSDDGELKI
S584	NPPKRDLSFLLDKKE
Y607	PKLDSTAYKSDDSSD
S609	LDSTAYKSDDSSDEG
S612	TAYKSDDSSDEGSLH
S613	AYKSDDSSDEGSLHI
S617	DDSSDEGSLHIDTDT
T622	EGSLHIDTDTKPSRN
T624	SLHIDTDTKPSRNAK
S636	NAKVKKESGSSAAGI
S650	ILDLLQASEEVGALE
S731	LKRTAKNSVDLEDYE
S756	DSDYVYPSLESDEDN
S759	YVYPSLESDEDNPVF
S776	RSKKRKGSDDAPYSP
S782	GSDDAPYSPTARVGP
S790	PTARVGPSVPRQDRP
T802	DRPVREGTRVASIET
S819	AAAAAKLSQQEEQKN
N831	QKNRKKKNTKRKPTP
S940	FLTQRRPSASSPNNT
S942	TQRRPSASSPNNTAA
S943	QRRPSASSPNNTAAK
T947	SASSPNNTAAKGKRT
K955	AAKGKRTKKGMATAK
K962	KKGMATAKQRLGKIL

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