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eNOS (human)

Overview eNOS endothelial constitutive nitric oxide synthase. Synthesizes nitric oxide (NO) from arginine and oxygen, which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Induced in humans by caloric restriction. Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN. Note: This description may include information from UniProtKB. Protein type: Amino Acid Metabolism - arginine and proline; Oxidoreductase; EC 1.14.13.39 Cellular Component: Golgi membrane; cytoskeleton; apical part of cell; cytoplasm; plasma membrane; nucleolus; caveola; nucleus; cytosol Molecular Function: actin monomer binding; calmodulin binding; protein binding; FAD binding; nitric-oxide synthase activity; FMN binding; iron ion binding; heme binding; NADP binding; cadmium ion binding Biological Process: response to peptide hormone stimulus; regulation of systemic arterial blood pressure by endothelin; negative regulation of smooth muscle cell proliferation; positive regulation of apoptosis; negative regulation of potassium ion transport; negative regulation of muscle hyperplasia; positive regulation of vasodilation; nitric oxide metabolic process; response to estradiol stimulus; negative regulation of cell proliferation; regulation of blood pressure; negative regulation of hydrolase activity; lipopolysaccharide-mediated signaling pathway; response to lipoprotein stimulus; angiogenesis; nitric oxide biosynthetic process; regulation of blood vessel size; aging; response to drug; mitochondrion organization and biogenesis; interaction with host; negative regulation of calcium ion transport; in utero embryonic development; positive regulation of guanylate cyclase activity; regulation of sodium ion transport; response to amino acid stimulus; ovulation from ovarian follicle; positive regulation of angiogenesis; response to hyperoxia; response to ethanol; response to mechanical stimulus; response to heat; response to cytokine stimulus; response to lead ion; response to hypoxia; arginine catabolic process; endothelial cell migration; blood vessel remodeling; regulation of nitric-oxide synthase activity; blood coagulation; smooth muscle hyperplasia; negative regulation of apoptosis; lung development Reference #:  P29474 (UniProtKB) Alt. Names/Synonyms: cNOS; Constitutive NOS; EC-NOS; ECNOS; Endothelial NOS; eNOS; nitric oxide synthase 3 (endothelial cell); Nitric oxide synthase, endothelial; NOS type III; NOS3; NOSIII Gene Symbols: NOS3 Molecular weight: 133,289 Da Basal Isoelectric point: 6.94  Predict pI for various phosphorylation states CST Pathways:  AMPK Signaling  |  Angiogenesis  |  PI3K/Akt Signaling Protein-Specific Antibodies or siRNAs from Cell Signaling Technology®

Select Structure to View Below eNOS 1M9J - A/B=67-481 (human) 1M9K - A/B=67-481 (human) 1M9M - A/B=67-481 (human) 1M9Q - A/B=67-481 (human) 1M9R - A/B=67-481 (human) 2LL7 - B=493-509 (human) 3EAH - A/B=66-492 (human) 3NOS - A/B=66-492 (human) 1D0C - A/B=39-482 (cow) 1D0O - A/B=39-482 (cow) 1D1V - A/B=39-482 (cow) 1D1W - A/B=39-482 (cow) 1D1X - A/B=39-482 (cow) 1D1Y - A/B=39-482 (cow) 1DM6 - A/B=39-482 (cow) 1DM7 - A/B=39-482 (cow) 1DM8 - A/B=39-482 (cow) 1DMI - A/B=39-482 (cow) 1DMJ - A/B=39-482 (cow) 1DMK - A/B=39-482 (cow) 1ED4 - A/B=39-482 (cow) 1ED5 - A/B=39-482 (cow) 1ED6 - A/B=39-482 (cow) 1FOI - A/B=39-482 (cow) 1FOJ - A/B=39-482 (cow) 1FOL - A/B=39-482 (cow) 1FOO - A/B=39-482 (cow) 1FOP - A/B=39-482 (cow) 1I83 - A/B=39-482 (cow) 1NSE - A/B=39-482 (cow) 1P6L - A/B=67-483 (cow) 1P6M - A/B=67-483 (cow) 1P6N - A/B=67-483 (cow) 1Q2O - A/B=67-482 (cow) 1RS8 - A/B=67-482 (cow) 1RS9 - A/B=67-482 (cow) 1ZZS - A/B=67-482 (cow) 1ZZT - A/B=67-482 (cow) 2G6O - A/B=67-482 (cow) 2HX2 - A/B=67-482 (cow) 2NSE - A/B=39-482 (cow) 3DQS - A/B=67-482 (cow) 3DQT - A/B=67-482 (cow) 3E7S - A/B=56-486 (cow) 3JWW - A/B=39-482 (cow) 3JWX - A/B=39-482 (cow) 3JWY - A/B=39-482 (cow) 3JWZ - A/B=39-482 (cow) 3N5P - A/B=39-482 (cow) 3N5Q - A/B=39-482 (cow) 3N5R - A/B=39-482 (cow) 3N5S - A/B=39-482 (cow) 3N5T - A/B=39-482 (cow) 3NLD - A/B=39-482 (cow) 3NLE - A/B=39-482 (cow) 3NLF - A/B=39-482 (cow) 3NLG - A/B=39-482 (cow) 3NLH - A/B=39-482 (cow) 3NLI - A/B=39-482 (cow) 3NLT - A/B=39-482 (cow) 3NLU - A/B=39-482 (cow) 3NSE - A/B=39-482 (cow) 3PNH - A/B=67-482 (cow) 3RQO - A/B=40-482 (cow) 3RQP - A/B=40-482 (cow) 4NSE - A/B=39-482 (cow) 5NSE - A/B=39-482 (cow) 6NSE - A/B=39-482 (cow) 7NSE - A/B=39-482 (cow) 8NSE - A/B=39-482 (cow) 9NSE - A/B=39-482 (cow)

STRING  |  Scansite  |  Phospho.ELM  |  NetworKIN  |  Pfam  |  RCSB PDB 1M9J 1M9K 1M9M 1M9Q 1M9R 2LL7 3EAH 3NOS  |  ENZYME  |  Phospho3D  |  DISEASE 104300 145500 163729 189800 601367  |  Source  |  UCSD-Nature  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene

	Sites Implicated In
apoptosis, inhibited: S1179‑p cell differentiation, altered: S1177‑p cell motility, altered: S1177‑p cytoskeletal reorganization: S1177‑p enzymatic activity, induced: S114‑p, S1177‑p, S1179‑p enzymatic activity, inhibited: T495‑p, Y657‑p intracellular localization: S114‑p, S1177‑p molecular association, regulation: T495‑p

	Modification Sites and Domains	Show Modification Legend
Click here to view phosphorylation modifications only

	Modification Sites in Parent Protein, Orthologs, and Isoforms	Show Modification Legend

SS  SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.	MS  MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.			human
0	1		K5-m1	___MGNLkSVAQEPG
3	1		Y81-p	WEVGSITyDTLSAQA
0	1		S102-p	TPRRCLGsLVFPRKL
16	1		S114-p	RKLQGRPsPGPPAPE
1	0		P118	GRPsPGPPAPEQLLS
0	1		Y210-p	SAQEMFTyICNHIKY
56	1		T495-p	TGITRKKtFKEVANA
0	2		S508-p	NAVKISAsLMGtVMA
0	2		T512-p	ISAsLMGtVMAKRVK
10	2		S615-p	SYKIRFNsISCSDPL
20	4		S633-p	WRRKRKEsSNTDSAG
2	1		Y657-p	FGLGSRAyPHFCAFA
0	1		S738-p	KRQRYRLsAQAEGLQ
0	1		K773-u	VENLQSSkSTRATIL
0	1		K834-u	VAVEQLEkGSPGGPP
0	1		T854-p	DPRLPPCtLRQALTF
0	1		K1085-u	SREPDNPkTYVQDIL
2	11		T1175-p	EVTSRIRtQsFsLQE
138	12		S1177-p	TSRIRtQsFsLQERQ
2	0		S1177-g	TSRIRtQsFsLQERQ
7	3		S1179-p	RIRtQsFsLQERQLR

	mouse
K5	___MGNLKSVGQEPG
Y80	WEVGSITYDTLSAQA
S101	TSRRCLGSLVFPRKL
T113	RKLQSRPTQGPSPTE
S117	SRPTQGPSPTEQLLG
Y209	TAQEMFTYICNHIKY
T494-p	AGITRKKtFKEVANA
S507	NAVKISASLMGTVMA
T511	ISASLMGTVMAKRVK
S614-p	SYKIRFNsVSCSDPL
S632-p	WRRKRKEsSNTDSAG
Y656-p	FGLGSRAyPHFCAFA
S737	KRQRYRLSTQAESLQ
K772	VENLQSSKSTRATIL
K833	VAVEQLEKGSPGGPP
T853	DPRLPPCTLRQALTY
K1084	SRDPGSPKTYVQDLL
T1174-p	EVTSRIRtQsFsLQE
S1176-p	TSRIRtQsFsLQERQ
S1176	TSRIRtQSFsLQERQ
S1178-p	RIRtQsFsLQERQLR

	rat
K5	___MGNLKSVGQEPG
Y80-p	WEVGSITyDTLSAQA
S101	TPRRCLGSLVFPRKL
T113	RKLQSRPTQGPsPTE
S117-p	SRPTQGPsPTEQLLG
Y209	TAQEMFTYICNHIKY
T494-p	TGITRKKtFKEVANA
S507	NAVKISASLMGTVMA
T511	ISASLMGTVMAKRVK
S614-p	SYKIRFNsVSCSDPL
S632-p	WRRKRKEsSNTDSAG
Y656	FGLGSRAYPHFCAFA
S737	KRQRYRLSTQAESLQ
K772	VENLQSSKSTRATIL
K833	VAVEQLEKGSPGGPP
T853	DPRLPPCTLRQALTY
K1084	SRDPGSPKTYVQDLL
T1174	EVTSRIRTQsFsLQE
S1176-p	TSRIRTQsFsLQERQ
S1176-g	TSRIRTQsFsLQERQ
S1178-p	RIRTQsFsLQERQLR

	rabbit
K5	___MGNLKSVGQEPG
Y87	WEVGSITYDTLSAQA
S108	TQRRCLGSLVFPRKL
T120	RKLQSRPTQGAPPPE
P124	SRPTQGAPPPEQLLV
Y216	SAQEMFTYICNHIKY
T501	TGITRKKTFKEVANA
S514	NAVKISASLMGTVMA
T518	ISASLMGTVMAKRVK
S621	SYKIRFNSVSCSDPL
S639	WRRKRKESSNTDSAG
Y663	FGLGSRAYPHFCAFA
S744	KRQRYRLSTQTEGLQ
K779	VENLQSSKSTRATIL
K840	VAVEQLEKGSPGGPP
S860	DPRLPPCSLRQALTF
K1091	SREPDSPKTYVQDIL
T1181	EVTSRIRTQsFSLQE
S1183-p	TSRIRTQsFSLQERQ
S1183	TSRIRTQSFSLQERQ
S1185	RIRTQsFSLQERQLR

	pig
K5	___MGNLKSVAQEPG
Y83	WEVGSITYDTLCAQS
S104	TPRRCLGSLVFPRKL
S116	RKLQSRPSQGPPPTE
P120	SRPSQGPPPTEQLLS
Y212	SAQEMFTYICNHIKY
T497-p	TGIARKKtFKEVANA
S510	NAVKISASLMGTVMA
T514	ISASLMGTVMAKRVK
S617	SYKIRFNSVSCSDPL
S635	WRRKRKESSNTDSAG
Y659-p	FGLGSRAyPHFCAFA
S740	KRQRYRLSAQAEGLQ
K775	VENLQSSKSTRATIL
K836	VAVEQLEKGSPGGPP
T856	DPRLPPCTLRQALTF
K1087	SREPDSPKTYVQDIL
T1177-p	EVTSRIRtQsFSLQE
S1179-p	TSRIRtQsFSLQERH
S1179	TSRIRtQSFSLQERH
S1181	RIRtQsFSLQERHLR

	cow
K5	___MGNLKSVGQEPG
Y83-p	WELGSITyDTLCAQS
S104	TPRCCLGSLVLPRKL
S116-p	RKLQTRPsPGPPPAE
P120	TRPsPGPPPAEQLLS
Y212	SAQEMFTYICNHIKY
T497-p	AGITRKKtFKEVANA
S510	NAVKISASLMGTLMA
T514	ISASLMGTLMAKRVK
S617-p	SYKIRFNsVSCSDPL
S635-p	WRRKRKEsSNTDSAG
Y659	FGLGSRAYPHFCAFA
S740	KRQRYRLSTQAEGLQ
K775	VENLQSSKSTRATIL
K836	VAVEQLEKGSPGGPP
T856	DPRLPPCTLRQALTF
K1087	SREPDSPKTYVQDIL
T1177-p	EVTSRIRtQsFsLQE
S1179-p	TSRIRtQsFsLQERH
S1179	TSRIRtQSFsLQERH
S1181-p	RIRtQsFsLQERHLR

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