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Protein Page:
eNOS (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
eNOS endothelial constitutive nitric oxide synthase. Synthesizes nitric oxide (NO) from arginine and oxygen, which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Induced in humans by caloric restriction. Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN. Note: This description may include information from UniProtKB.
Protein type: Amino Acid Metabolism - arginine and proline; Oxidoreductase; EC 1.14.13.39
Cellular Component: Golgi membrane; cytoskeleton; cytoplasm; plasma membrane; caveola; cytosol; nucleus
Molecular Function: calmodulin binding; actin monomer binding; protein binding; FAD binding; FMN binding; nitric-oxide synthase activity; NADPH-hemoprotein reductase activity; iron ion binding; heme binding; NADP binding; cadmium ion binding
Biological Process: regulation of systemic arterial blood pressure by endothelin; negative regulation of potassium ion transport; negative regulation of muscle hyperplasia; positive regulation of vasodilation; nitric oxide metabolic process; negative regulation of cell proliferation; regulation of blood pressure; negative regulation of hydrolase activity; regulation of the force of heart contraction by chemical signal; lipopolysaccharide-mediated signaling pathway; negative regulation of blood pressure; angiogenesis; regulation of blood vessel size; nitric oxide biosynthetic process; interaction with host; mitochondrion organization and biogenesis; negative regulation of calcium ion transport; in utero embryonic development; positive regulation of guanylate cyclase activity; regulation of sodium ion transport; ovulation from ovarian follicle; positive regulation of angiogenesis; response to heat; endothelial cell migration; arginine catabolic process; blood vessel remodeling; regulation of nitric-oxide synthase activity; blood coagulation; nitric oxide mediated signal transduction; lung development; smooth muscle hyperplasia
Reference #:  P29474 (UniProtKB)
Alt. Names/Synonyms: cNOS; Constitutive NOS; EC-NOS; ECNOS; Endothelial NOS; eNOS; nitric oxide synthase 3 (endothelial cell); Nitric oxide synthase, endothelial; NOS type III; NOS3; NOSIII
Gene Symbols: NOS3
Molecular weight: 133,289 Da
Basal Isoelectric point: 6.94  Predict pI for various phosphorylation states
CST Pathways:  AMPK Signaling  |  Angiogenesis  |  PI3K/Akt Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

eNOS

Protein Structure Not Found.


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Sites Implicated In
apoptosis, inhibited: S1179‑p
cell differentiation, altered: S1177‑p
cell motility, altered: S1177‑p
cytoskeletal reorganization: S1177‑p
enzymatic activity, induced: S114‑p, S1177‑p, S1179‑p
enzymatic activity, inhibited: T495‑p, Y657‑p
intracellular localization: S114‑p, S1177‑p
molecular association, regulation: T495‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 K5-m1 ___MGNLkSVAQEPG
0 1 T33-p CGKQGPAtPAPEPSR
1 0 L46 SRAPASLLPPAPEHs
0 1 S53-p LPPAPEHsPPSSPLT
1 0 S56 APEHsPPSSPLTQPP
4 1 Y81-p WEVGSITyDTLSAQA
0 1 S102-p TPRRCLGsLVFPRKL
17 2 S114-p RKLQGRPsPGPPAPE
1 0 P118 GRPsPGPPAPEQLLS
0 1 Y210-p SAQEMFTyICNHIKY
1 0 K494 GTGITRKKtFKEVAN
56 1 T495-p TGITRKKtFKEVANA
1 0 K504 KEVANAVKISAsLMG
0 2 S508-p NAVKISAsLMGtVMA
0 2 T512-p ISAsLMGtVMAKRVK
1 0 S600 MSGPYNSSPRPEQHK
10 2 S615-p SYKIRFNsISCSDPL
21 5 S633-p WRRKRKEsSNTDsAG
0 1 S638-p KEsSNTDsAGALGTL
2 1 Y657-p FGLGSRAyPHFCAFA
0 1 S738-p KRQRYRLsAQAEGLQ
0 1 K773-ub VENLQSSkSTRATIL
0 1 K834-ub VAVEQLEkGsPGGPP
0 1 S836-p VEQLEkGsPGGPPPG
0 1 T854-p DPRLPPCtLRQALTF
0 1 K1085-ub SREPDNPkTYVQDIL
0 1 T1094-p YVQDILRtELAAEVH
2 12 T1175-p EVTSRIRtQsFsLQE
141 14 S1177-p TSRIRtQsFsLQERQ
2 0 S1177-gl TSRIRtQsFsLQERQ
7 3 S1179-p RIRtQsFsLQERQLR
9575 : Phospho-eNOS (Ser113) Antibody
9574 : Phospho-eNOS (Thr495) Antibody
9570 : Phospho-eNOS (Ser1177) (C9C3) Rabbit mAb
9571 : Phospho-eNOS (Ser1177) Antibody
  mouse

 
K5 ___MGNLKSVGQEPG
S33 CGKQGPASPAPEPSQ
T48 APAPPSPTRPAPDHS
S55 TRPAPDHSPPLTRPP
- gap
Y80 WEVGSITYDTLSAQA
S101 TSRRCLGSLVFPRKL
T113 RKLQSRPTQGPSPTE
S117 SRPTQGPSPTEQLLG
Y209 TAQEMFTYICNHIKY
K493 GAGITRKKtFKEVAN
T494-p AGITRKKtFKEVANA
K503 KEVANAVKISASLMG
S507 NAVKISASLMGTVMA
T511 ISASLMGTVMAKRVK
S599 MSGPYNSSPRPEQHK
S614-p SYKIRFNsVSCSDPL
S632-p WRRKRKEsSNTDSAG
S637 KEsSNTDSAGALGTL
Y656-p FGLGSRAyPHFCAFA
S737 KRQRYRLSTQAESLQ
K772 VENLQSSKSTRATIL
K833 VAVEQLEKGSPGGPP
S835 VEQLEKGSPGGPPPG
T853 DPRLPPCTLRQALTY
K1084 SRDPGSPKTYVQDLL
T1093 YVQDLLRTELAAEVH
T1174-p EVTSRIRtQsFsLQE
S1176-p TSRIRtQsFsLQERQ
S1176 TSRIRtQSFsLQERQ
S1178-p RIRtQsFsLQERQLR
9574 : Phospho-eNOS (Thr495) Antibody
9570 : Phospho-eNOS (Ser1177) (C9C3) Rabbit mAb
9571 : Phospho-eNOS (Ser1177) Antibody
  rat

 
K5 ___MGNLKSVGQEPG
S33 CGKQGPASPAPEPSQ
T48 APVPPSPTRPAPDHS
S55 TRPAPDHSPPLTRPP
- gap
Y80-p WEVGSITyDTLSAQA
S101 TPRRCLGSLVFPRKL
T113 RKLQSRPTQGPsPTE
S117-p SRPTQGPsPTEQLLG
Y209 TAQEMFTYICNHIKY
K493 GTGITRKKtFKEVAN
T494-p TGITRKKtFKEVANA
K503 KEVANAVKISASLMG
S507 NAVKISASLMGTVMA
T511 ISASLMGTVMAKRVK
S599 MSGPYNSSPRPEQHK
S614-p SYKIRFNsVSCSDPL
S632-p WRRKRKEsSNTDSAG
S637 KEsSNTDSAGALGTL
Y656 FGLGSRAYPHFCAFA
S737 KRQRYRLSTQAESLQ
K772 VENLQSSKSTRATIL
K833 VAVEQLEKGSPGGPP
S835 VEQLEKGSPGGPPPG
T853 DPRLPPCTLRQALTY
K1084 SRDPGSPKTYVQDLL
T1093 YVQDLLRTELAAEVH
T1174-p EVTSRIRtQsFsLQE
S1176-p TSRIRtQsFsLQERQ
S1176-gl TSRIRtQsFsLQERQ
S1178-p RIRtQsFsLQERQLR
9570 : Phospho-eNOS (Ser1177) (C9C3) Rabbit mAb
  rabbit

 
K5 ___MGNLKSVGQEPG
S33 CGKQGPASPAPEPSR
T48 APASPPPTPPAPDHS
S55 TPPAPDHSPKPSSNS
S62 SPKPSSNSPPLTRPP
Y87 WEVGSITYDTLSAQA
S108 TQRRCLGSLVFPRKL
T120 RKLQSRPTQGAPPPE
P124 SRPTQGAPPPEQLLV
Y216 SAQEMFTYICNHIKY
K500 GTGITRKKTFKEVAN
T501 TGITRKKTFKEVANA
K510 KEVANAVKISASLMG
S514 NAVKISASLMGTVMA
T518 ISASLMGTVMAKRVK
S606 MSGPYNSSPRPEQHK
S621 SYKIRFNSVSCSDPL
S639 WRRKRKESSNTDSAG
S644 KESSNTDSAGALGTL
Y663 FGLGSRAYPHFCAFA
S744 KRQRYRLSTQTEGLQ
K779 VENLQSSKSTRATIL
K840 VAVEQLEKGSPGGPP
S842 VEQLEKGSPGGPPPS
S860 DPRLPPCSLRQALTF
K1091 SREPDSPKTYVQDIL
T1100 YVQDILRTELASEVH
T1181 EVTSRIRTQsFSLQE
S1183-p TSRIRTQsFSLQERQ
S1183 TSRIRTQSFSLQERQ
S1185 RIRTQsFSLQERQLR
  pig

 
K5 ___MGNLKSVAQEPG
S33 CGKQGPASPAPEASQ
P46 SQAPAPAPAPAPDHS
S53 PAPAPDHSPAPNSPP
S58 DHSPAPNSPPLTRPP
Y83 WEVGSITYDTLCAQS
S104 TPRRCLGSLVFPRKL
S116 RKLQSRPSQGPPPTE
P120 SRPSQGPPPTEQLLS
Y212 SAQEMFTYICNHIKY
K496 GTGIARKKtFKEVAN
T497-p TGIARKKtFKEVANA
K506 KEVANAVKISASLMG
S510 NAVKISASLMGTVMA
T514 ISASLMGTVMAKRVK
S602 MSGPYNGSPRPEQHR
S617 SYKIRFNSVSCSDPL
S635 WRRKRKESSNTDSAG
S640 KESSNTDSAGALGTL
Y659-p FGLGSRAyPHFCAFA
S740 KRQRYRLSAQAEGLQ
K775 VENLQSSKSTRATIL
K836 VAVEQLEKGSPGGPP
S838 VEQLEKGSPGGPPPS
T856 DPRLPPCTLRQALTF
K1087 SREPDSPKTYVQDIL
T1096 YVQDILRTELAAEVH
T1177-p EVTSRIRtQsFSLQE
S1179-p TSRIRtQsFSLQERH
S1179 TSRIRtQSFSLQERH
S1181 RIRtQsFSLQERHLR
  cow

 
K5 ___MGNLKSVGQEPG
S33 CGKQGPASPAPEPSR
T46-p SRAPAPAtPHAPDHS
S53 tPHAPDHSPAPNsPT
S58-p DHSPAPNsPTLTRPP
Y83-p WELGSITyDTLCAQS
S104 TPRCCLGSLVLPRKL
S116-p RKLQTRPsPGPPPAE
P120 TRPsPGPPPAEQLLS
Y212 SAQEMFTYICNHIKY
K496-ac GAGITRKktFKEVAN
T497-p AGITRKktFKEVANA
K506-ac KEVANAVkISASLMG
S510 NAVkISASLMGTLMA
T514 ISASLMGTLMAKRVK
S602-p MSGPYNSsPRPEQHK
S617-p SYKIRFNsVSCSDPL
S635-p WRRKRKEsSNTDSAG
S640 KEsSNTDSAGALGTL
Y659 FGLGSRAYPHFCAFA
S740 KRQRYRLSTQAEGLQ
K775 VENLQSSKSTRATIL
K836 VAVEQLEKGSPGGPP
S838 VEQLEKGSPGGPPPS
T856 DPRLPPCTLRQALTF
K1087 SREPDSPKTYVQDIL
T1096 YVQDILRTELAAEVH
T1177-p EVTSRIRtQsFsLQE
S1179-p TSRIRtQsFsLQERH
S1179 TSRIRtQSFsLQERH
S1181-p RIRtQsFsLQERHLR
9575 : Phospho-eNOS (Ser113) Antibody
9570 : Phospho-eNOS (Ser1177) (C9C3) Rabbit mAb
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