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Protein Page:
RPA1 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
RPA1 Plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing. Heterotrimer composed of RPA1, RPA2 and RPA3 (canonical replication protein A complex). Component of the alternative replication protein A complex (aRPA) composed of RPA1, RPA3 and RPA4. The DNA-binding activity may reside exclusively on the RPA1 subunit. Interacts with RIPK1 and XPA. Interacts with RPA4. Interacts with the polymerase alpha subunit POLA1/p180; this interaction stabilizes the replicative complex and reduces the misincorporation rate of DNA polymerase alpha by acting as a fidelity clamp. Interact with RAD51 and SENP6 to regulate DNA repair. Interacts with HELB; this interaction promotes HELB recruitement to chromatin following DNA damage. Belongs to the replication factor A protein 1 family. Note: This description may include information from UniProtKB.
Protein type: DNA binding protein
Cellular Component: nucleoplasm; male germ cell nucleus; lateral element; PML body; DNA replication factor A complex; cytoplasm; nucleolus; nucleus; actin cytoskeleton
Molecular Function: protein binding; metal ion binding; damaged DNA binding; chromatin binding; single-stranded DNA binding
Biological Process: meiosis; mismatch repair; in utero embryonic development; DNA strand elongation during DNA replication; DNA repair; double-strand break repair via homologous recombination; DNA recombination; telomere maintenance via semi-conservative replication; DNA recombinase assembly; base-excision repair; homeostasis of number of cells within a tissue; nucleotide-excision repair; double-strand break repair; transcription-coupled nucleotide-excision repair; positive regulation of cell proliferation; telomere maintenance via recombination; hemopoiesis; nucleotide-excision repair, DNA damage removal; mitotic cell cycle; nucleotide-excision repair, DNA gap filling; DNA-dependent DNA replication; DNA replication; telomere maintenance; G1/S transition of mitotic cell cycle
Reference #:  P27694 (UniProtKB)
Alt. Names/Synonyms: HSSB; MST075; MSTP075; REPA1; Replication factor A protein 1; Replication protein A 70 kDa DNA-binding subunit; replication protein A1; replication protein A1, 70kDa; RF-A; RF-A protein 1; RFA1; RP-A; RP-A p70; RPA1; RPA70; Single-stranded DNA-binding protein
Gene Symbols: RPA1
Molecular weight: 68,138 Da
Basal Isoelectric point: 6.92  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

RPA1

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 S6-p __MVGQLsEGAIAAI
0 1 K22-ub QKGDTNIkPILQVIN
0 1 T35-p INIRPITtGNsPPRY
0 2 S38-p RPITtGNsPPRYRLL
0 6 K88-ub RFIVNTLkDGRRVVI
0 1 K157-ub GMGSTVSkAYGASkT
0 22 K163-ac SkAYGASkTFGkAAG
0 6 K163-m1 SkAYGASkTFGkAAG
0 1 K163-ub SkAYGASkTFGkAAG
0 7 K167-ac GASkTFGkAAGPSLs
0 3 K167-ub GASkTFGkAAGPSLs
0 2 S174-p kAAGPSLsHTsGGtQ
0 2 S177-p GPSLsHTsGGtQskV
0 21 T180-p LsHTsGGtQskVVPI
0 1 S182-p HTsGGtQskVVPIAS
0 1 K183-ub TsGGtQskVVPIASL
0 6 T191-p VVPIASLtPYQSkWT
0 1 K196-ac SLtPYQSkWTICARV
0 1 K196-ub SLtPYQSkWTICARV
0 1 S207-p CARVTNKsQIRTWSN
0 2 K244-ub AFNEQVDkFFPLIEV
0 2 K259-ac NKVYYFSkGTLKIAN
0 1 K267-ac GTLKIANkQFTAVKN
0 2 S315-p LENKSKDsLVDIIGI
0 1 K367-ub LWGEDADkFDGSRQP
0 1 S371 DADkFDGSRQPVLAI
0 11 S384-p AIKGARVsDFGGRSL
0 32 K410-ub PDIPEAYkLRGWFDA
0 3 K431-ub GVSISDLkSGGVGGS
0 2 K443-ub GGSNTNWkTLYEVKS
0 2 K458-ub ENLGQGDkPDyFSSV
0 6 Y461-p GQGDkPDyFSSVATV
0 2 Y478-p LRKENCMyQACPtQD
0 1 T483-p CMyQACPtQDCNKkV
0 1 K489-ac PtQDCNKkVIDQQNG
0 5 K489-ub PtQDCNKkVIDQQNG
0 1 K502-ac NGLYRCEkCDTEFPN
0 7 K553-ub YLGELKDkNEQAFEE
0 1 K577-ub FIFRVRVkVETyNDE
0 6 Y581-p VRVkVETyNDESRIk
0 2 K588-ub yNDESRIkAtVMDVk
0 1 T590-p DESRIkAtVMDVkPV
0 3 K595-ub kAtVMDVkPVDyREY
0 1 Y599-p MDVkPVDyREYGRRL
  mouse

 
S6 __MVGHLSEGAIEVM
K22 QQENTSIKPILQVIN
T35 INIRPISTGNRSPRY
R38 RPISTGNRSPRYRLL
K88-ub KFIVNTLkDGRKVVV
K166 GMGSTAAKAYGASKP
K172 AKAYGASKPFGKPAG
K172 AKAYGASKPFGKPAG
K172 AKAYGASKPFGKPAG
K176 GASKPFGKPAGTGLL
K176 GASKPFGKPAGTGLL
L183 KPAGTGLLQPSGGTQ
S186 GTGLLQPSGGTQSKV
T189 LLQPSGGTQSKVVPI
S191 QPSGGTQSKVVPIAS
K192 PSGGTQSKVVPIASL
T200 VVPIASLTPYQSKWT
K205 SLTPYQSKWTICARV
K205 SLTPYQSKWTICARV
S216 CARVTNKSQIRTWSN
K253 AFNEQVDKFFPLIEV
K268 NKVYYFSKGALKIAN
K276 GALKIANKQFSAVKN
A324 LESKAKDALVDIIGI
K376 LWGEDADKFDGsRQP
S380-p DADKFDGsRQPVMAI
S393-p AIKGARVsDFGGRSL
K419 PDIPEAYKLRGWFDS
R440 GVSISDHRSGGAGGG
K452 GGGNTNWKTLHEAKS
K467-ub ENLGQGDkADyFSTV
Y470-p GQGDkADyFSTVAAV
Y487 LRKENCMYQACPTQD
T492 CMYQACPTQDCNKkV
K498 PTQDCNKKVIDQQNG
K498-ub PTQDCNKkVIDQQNG
K511 NGLYRCEKCDREFPN
K562-ub YLGELKEkNEQAFEE
K586 FTFRIRVKLETYNDE
Y590 IRVKLETYNDESRIK
K597 YNDESRIKATVMDVk
T599 DESRIKATVMDVkPV
K604-ub KATVMDVkPVDFRDY
F608 MDVkPVDFRDYGRRL
  rat

 
- gap
K9 QQGDTSIKPVLQVIN
T22 INIRPITTGNSPPRY
S25 RPITTGNSPPRYRLL
K75 RFIVNTLKDGRRVVI
K144 GVGSTVAKAYGASKP
K150 AKAYGASKPFGKPAG
K150 AKAYGASKPFGKPAG
K150 AKAYGASKPFGKPAG
K154 GASKPFGKPAGTGLL
K154 GASKPFGKPAGTGLL
L161 KPAGTGLLQPTSGTQ
T164 GTGLLQPTSGTQSKV
T167 LLQPTSGTQSKVVPI
S169 QPTSGTQSKVVPIAS
K170 PTSGTQSKVVPIASL
T178 VVPIASLTPYQSKWT
K183 SLTPYQSKWTICARV
K183 SLTPYQSKWTICARV
S194 CARVTNKSQIRTWSN
K231 AFNEQVDKFFPLIEV
K246 NKVYYFSKGTLKIAN
K254 GTLKIANKQFSAVKN
S302 LESKSKDSLVDIIGI
K354 LWGEDADKFDGSRQP
S358 DADKFDGSRQPVMAI
S371 AIKGARVSDFGGRSL
K397 PDIPEAYKLRGWHYV
R489 GVSISDLRSGGTGGS
K501 GGSNTNWKTLYEAKS
K516 ENLGQGDKADYFSTV
Y519 GQGDKADYFSTVATV
Y536-p LRKENCMyQACPTQD
T541 CMyQACPTQDCNKKV
K547 PTQDCNKKVIDQQNG
K547 PTQDCNKKVIDQQNG
K560 NGLYRCEKCDREFPN
K611 YLGELKEKVGCVFVS
K643 FTFRIRVKLETYNDE
Y647 IRVKLETYNDESRIK
K654 YNDESRIKATVIDVK
T656 DESRIKATVIDVKPV
K661 KATVIDVKPVDYRDY
Y665 IDVKPVDYRDYSKRL
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