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Protein Page:
DFFA (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
g O-GlcNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
DFFA Inhibitor of the caspase-activated DNase (DFF40). Heterodimer of DFFA and DFFB. 2 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Hydrolase
Cellular Component: nucleoplasm; cytoplasm; nuclear chromatin; cytosol; nucleus
Molecular Function: deoxyribonuclease activity
Biological Process: apoptosis; induction of apoptosis; DNA fragmentation during apoptosis; cell structure disassembly during apoptosis; negative regulation of apoptosis
Reference #:  O00273 (UniProtKB)
Alt. Names/Synonyms: DFF-45; DFF1; DFF45; DFFA; DNA fragmentation factor 45 kDa subunit; DNA fragmentation factor subunit alpha; DNA fragmentation factor, 45kDa, alpha polypeptide; ICAD; Inhibitor of CAD
Gene Symbols: DFFA
Molecular weight: 36,522 Da
Basal Isoelectric point: 4.68  Predict pI for various phosphorylation states
CST Pathways:  Apoptosis  |  Death Receptor Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

DFFA
Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


  MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


        human

► Hide Isoforms 		 
0 9 S28-p CLLRRNYsREQHGVA
0 31 Y75-p TIVDDDDyFLCLPSN
0 10 S107-p DGGTAWIsQEsFDVD
0 3 S110-p TAWIsQEsFDVDETD
0 1 K189-u REEVRQSkQLLQLYL
0 5 K201-u LYLQALEkEGSLLSK
0 3 S232-p TGISRETsSDVALAS
0 1 K248 ILTALREKQAPELSL
0 1 S256 QAPELSLSsQDLELV
0 6 S257-p APELSLSsQDLELVT
0 1 S310-p LHSLRSIsASKAsPP
0 8 A314 RSIsASKAsPPGDLQ
0 8 S315-p SIsASKAsPPGDLQN
0 13 K324-a PGDLQNPkRARQDPT
  DFFA iso2  
S28-p CLLRRNYsREQHGVA
Y75-p TIVDDDDyFLCLPSN
S107 DGGTAWISQESFDVD
S110 TAWISQESFDVDETD
K189 REEVRQSKQLLQLYL
K201 LYLQALEKEGSLLSK
S232 TGISRETSSDVALAS
K248-u ILTALREkQAPELSL
S256-p QAPELSLssQDLEVG
S257-p APELSLssQDLEVGG
- gap
- gap
- gap
- gap
  mouse

► Hide Isoforms 		 
S28-p CLLRRNHsRDQHGVA
Y75 TIVDDDDYFLCLPSN
S107 DGGTAWVSQEsFEAD
S110-p TAWVSQEsFEADEPD
K189 REEARQSKQLLELYL
K201 LYLQALEKEGNILSN
A232 TGVGREMASEVLLRS
K248 ILTTLKEKPAPELSL
S256 PAPELSLSSQDLESV
S257 APELSLSSQDLESVS
S310 LHSLRNLSARRsPLP
S314-p RNLSARRsPLPGEPQ
P315 NLSARRsPLPGEPQR
K324 PGEPQRPKRAKRDSS
  DFFA iso2  
S28 CLLRRNHSRDQHGVA
Y75 TIVDDDDYFLCLPSN
S107 DGGTAWVSQESFEAD
S110 TAWVSQESFEADEPD
K189 REEARQSKQLLELYL
K201 LYLQALEKEGNILSN
A232 TGVGREMASEVLLRS
K248 ILTTLKEKPAPELSL
S256 PAPELSLSSQDLEVG
S257 APELSLSSQDLEVGK
- gap
- gap
- gap
- gap
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