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Protein Page:
PYCARD (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
PYCARD Promotes caspase-mediated apoptosis. This proapoptotic activity is mediated predominantly through the activation of caspase-9. May be a component of the inflammasome, a protein complex which also includes NALP2, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases. Forms complexes with other DAPIN domain-containing proteins. Interacts with CIAS1/PYPAF1 and PYDC1. Widely expressed at low levels. Detected in peripheral blood leukocytes, lung, small intestine, spleen, thymus, colon and at lower levels in placenta, liver and kidney. Very low expression in skeletal muscle, heart and brain. Detected in the leukemia cell lines HL-60 and U-937, but not in Jurkat T- cell lymphoma and Daudi Burkitt's lymphoma. Detected in the melanoma cell line WM35, but not in WM793. Not detected in HeLa cervical carcinoma cells and MOLT-4 lymphocytic leukemia cells. 3 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 16p11.2
Cellular Component: cell soma; mitochondrion; endoplasmic reticulum; cytoplasm; nucleolus; extracellular region; IkappaB kinase complex; nucleus; cytosol
Molecular Function: protein binding; protein homodimerization activity; protease binding; caspase activator activity; Pyrin domain binding
Biological Process: myeloid dendritic cell activation during immune response; apoptosis; positive regulation of apoptosis; positive regulation of caspase activity; positive regulation of JNK cascade; regulation of protein stability; positive regulation of interleukin-1 beta secretion; defense response to Gram-negative bacterium; positive regulation of activated T cell proliferation; signal transduction; activation of NF-kappaB transcription factor; tumor necrosis factor-mediated signaling pathway; positive regulation of phagocytosis; inflammatory response; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; defense response to virus; regulation of Rac GTPase activity; positive regulation of adaptive immune response; caspase activation; interleukin-1 beta production; positive regulation of interleukin-6 production; negative regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of tumor necrosis factor production; negative regulation of interferon-beta production; myeloid dendritic cell activation; positive regulation of interferon-gamma production; positive regulation of actin filament polymerization; inhibition of NF-kappaB transcription factor; regulation of inflammatory response; innate immune response; positive regulation of transcription factor activity; positive regulation of T cell activation; positive regulation of defense response to virus by host; activation of innate immune response; positive regulation of antigen processing and presentation of peptide antigen via MHC class II
Reference #:  Q9ULZ3 (UniProtKB)
Alt. Names/Synonyms: Apoptosis-associated speck-like protein containing a CARD; ASC; CARD5; caspase recruitment domain protein 5; Caspase recruitment domain-containing protein 5; hASC; MGC10332; PYCARD; PYD and CARD domain containing; PYD and CARD domain-containing protein; Target of methylation-induced silencing 1; target of methylation-induced silencing-1; TMS; TMS-1; TMS1
Gene Symbols: PYCARD
Molecular weight: 21,627 Da
Basal Isoelectric point: 5.95  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
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PYCARD

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 6 K21-ac NLTAEELkkFKLKLL
0 2 K21-ub NLTAEELkkFKLKLL
0 2 K22-ub LTAEELkkFKLKLLS
0 1 S46-p IPRGALLsMDALDLT
0 1 K109-ub APPQSAAkPGLHFID
0 1 K139-ub LLDALYGkVLTDEQy
1 3 Y146-p kVLTDEQyQAVRAEP
0 1 R182 DLLLQALRESQSYLV
0 8 S195 LVEDLERS_______
  mouse

 
K21-ac NLSGDELkKFKMKLL
K21 NLSGDELKKFKMKLL
K22 LSGDELkKFKMKLLT
Q46 IPRGALLQMDAIDLT
R108 VPAQSTARTGHFVDQ
S137 VLDALHGSVLTEGQy
Y144-p SVLTEGQyQAVRAET
K180-ub DSLLQALkEIHPYLV
S193-p LVMDLEQs_______
  rat

 
K21 NLTADEFKKFKMKLL
K21 NLTADEFKKFKMKLL
K22 LTADEFKKFKMKLLT
Q46 IPRGALLQMDPIDLT
- under review  
N137 LLDALYGNVLTEGQY
Y144 NVLTEGQYQAVRAET
R180 NLFLEALRQTQPYLV
S193-p LVTDLEQs_______
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