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Protein Page:
GJA1 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
GJA1 an integral membrane protein of the connexin family, alpha-type (group II) subfamily. Hexamers of connexin-43 form connexons, which aggregate together to form gap junctions, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. Note: This description may include information from UniProtKB.
Protein type: Membrane protein, multi-pass; Membrane protein, integral; Channel, misc.; Motility/polarity/chemotaxis
Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; integral to plasma membrane; lysosome; early endosome; fascia adherens; lipid raft; Golgi membrane; multivesicular body; connexon complex; mitochondrial outer membrane; plasma membrane; gap junction
Molecular Function: signal transducer activity; protein binding; ion transmembrane transporter activity; SH3 domain binding; gap junction channel activity; PDZ domain binding
Biological Process: ATP transport; response to peptide hormone stimulus; chronic inflammatory response; positive regulation of I-kappaB kinase/NF-kappaB cascade; gap junction assembly; apoptosis; heart development; regulation of calcium ion transport; signal transduction; positive regulation of vasodilation; vascular transport; protein oligomerization; elevation of cytosolic calcium ion concentration; muscle contraction; cell-cell signaling; transport; negative regulation of cardiac muscle cell proliferation; positive regulation of glomerular filtration; positive regulation of vasoconstriction; positive regulation of protein catabolic process; transmembrane transport; neurite morphogenesis; response to pH
Reference #:  P17302 (UniProtKB)
Alt. Names/Synonyms: connexin 43; Connexin-43; Cx43; CXA1; DFNB38; Gap junction 43 kDa heart protein; Gap junction alpha-1 protein; gap junction protein, alpha 1, 43kDa; gap junction protein, alpha-like; GJA1; GJAL; HSS; ODDD
Gene Symbols: GJA1
Molecular weight: 43,008 Da
Basal Isoelectric point: 8.96  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

GJA1

Protein Structure Not Found.


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Sites Implicated In
cell growth, altered: S262‑p
cell motility, altered: S368‑p
cytoskeletal reorganization: S279‑p, S282‑p
activity, inhibited: S368‑p
intracellular localization: S255‑p, S262‑p, S279‑p, S282‑p
molecular association, regulation: S262‑p, Y265‑p, S373‑p
protein degradation: S279‑p, S282‑p, Y286‑p
protein processing: Y265‑p
ubiquitination: S279‑p, S282‑p, Y286‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 S5 ___MGDWSALGkLLD
0 2 K9-ub GDWSALGkLLDKVQA
0 1 K108 RKEEKLNKKEEELKV
0 16 K109 KEEKLNKKEEELKVA
0 6 K128-ub VNVDMHLkQIEIKKF
0 1 K133 HLkQIEIKKFKyGIE
0 1 K134 LkQIEIKKFKyGIEE
0 2 K136 QIEIKKFKyGIEEHG
0 18 Y137-p IEIKKFKyGIEEHGK
0 1 K144 yGIEEHGKVKMRGGL
0 2 K243-ub VKDRVKGksDPyHAt
1 7 S244-p KDRVKGksDPyHAts
8 253 Y247-p VKGksDPyHAtsGAL
0 5 T250-p ksDPyHAtsGALsPA
0 4 S251-p sDPyHAtsGALsPAk
15 12 S255-p HAtsGALsPAkDCGs
2 5 A257 tsGALsPAkDCGsQk
0 35 K258-ub sGALsPAkDCGsQky
17 9 S262-p sPAkDCGsQkyAyFN
0 15 K264-ub AkDCGsQkyAyFNGC
12 11 Y265-p kDCGsQkyAyFNGCS
0 10 Y267-p CGsQkyAyFNGCSsP
0 2 S273-p AyFNGCSsPtAPLsP
0 1 T275-p FNGCSsPtAPLsPMs
22 1 S279-p SsPtAPLsPMsPPGy
21 2 S282-p tAPLsPMsPPGykLV
1 12 Y286-p sPMsPPGykLVtGDR
0 7 K287-ub PMsPPGykLVtGDRN
0 2 T290-p PPGykLVtGDRNNss
2 8 S296-p VtGDRNNssCRNyNk
3 7 S297-p tGDRNNssCRNyNkQ
0 6 Y301-p NNssCRNyNkQAsEQ
0 27 K303-ub ssCRNyNkQAsEQNW
2 14 S306-p RNyNkQAsEQNWANy
0 308 Y313-p sEQNWANysAEQNRM
1 6 S314-p EQNWANysAEQNRMG
7 7 S325-p NRMGQAGstIsNsHA
0 7 T326-p RMGQAGstIsNsHAQ
7 7 S328-p GQAGstIsNsHAQPF
7 9 S330-p AGstIsNsHAQPFDF
0 1 N341 PFDFPDDNQNskkLA
0 1 S344-p FPDDNQNskkLAAGH
0 12 K345-ub PDDNQNskkLAAGHE
0 7 K346-ub DDNQNskkLAAGHEL
5 2 S364-p AIVDQRPssRAssRA
9 9 S365-p IVDQRPssRAssRAs
22 6 S368-p QRPssRAssRAssRP
12 6 S369-p RPssRAssRAssRPR
2 3 S372-p sRAssRAssRPRPDD
9 2 S373-p RAssRAssRPRPDDL
3511 : Phospho-Connexin 43 (Ser368) Antibody
  mouse

 
S5-p ___MGDWsALGkLLD
K9-ub GDWsALGkLLDKVQA
K108-ub RKEEKLNkkEEELKV
K109-ub KEEKLNkkEEELKVA
K128-ub VNVEMHLkQIEIkKF
K133-ub HLkQIEIkKFkyGIE
K134 LkQIEIkKFkyGIEE
K136-ub QIEIkKFkyGIEEHG
Y137-p IEIkKFkyGIEEHGk
K144-ub yGIEEHGkVKMRGGL
R243 VKDRVKGRSDPyHAt
S244 KDRVKGRSDPyHAtT
Y247-p VKGRSDPyHAtTGPL
T250-p RSDPyHAtTGPLsPs
T251 SDPyHAtTGPLsPsk
S255-p HAtTGPLsPskDCGs
S257-p tTGPLsPskDCGsPk
K258-ub TGPLsPskDCGsPky
S262-p sPskDCGsPkyAyFN
K264-ub skDCGsPkyAyFNGC
Y265-p kDCGsPkyAyFNGCS
Y267-p CGsPkyAyFNGCSsP
S273-p AyFNGCSsPtAPLsP
T275-p FNGCSsPtAPLsPMs
S279-p SsPtAPLsPMsPPGy
S282-p tAPLsPMsPPGykLV
Y286-p sPMsPPGykLVtGDR
K287-ub PMsPPGykLVtGDRN
T290-p PPGykLVtGDRNNss
S296-p VtGDRNNssCRNYNk
S297-p tGDRNNssCRNYNkQ
Y301 NNssCRNYNkQAsEQ
K303-ub ssCRNYNkQAsEQNW
S306-p RNYNkQAsEQNWANy
Y313-p sEQNWANysAEQNRM
S314-p EQNWANysAEQNRMG
S325-p NRMGQAGstIsNsHA
T326-p RMGQAGstIsNsHAQ
S328-p GQAGstIsNsHAQPF
S330-p AGstIsNsHAQPFDF
S341-p PFDFPDDsQNAkkVA
A344 FPDDsQNAkkVAAGH
K345-ub PDDsQNAkkVAAGHE
K346-ub DDsQNAkkVAAGHEL
S364-p AIVDQRPssRAssRA
S365-p IVDQRPssRAssRAs
S368-p QRPssRAssRAssRP
S369-p RPssRAssRAssRPR
S372-p sRAssRAssRPRPDD
S373-p RAssRAssRPRPDDL
3511 : Phospho-Connexin 43 (Ser368) Antibody
  rat

 
S5 ___MGDWSALGKLLD
K9 GDWSALGKLLDKVQA
K108 RKEEKLNKkEEELKV
K109-ub KEEKLNKkEEELKVA
K128-ub VNVEMHLkQIEIKkF
K133 HLkQIEIKkFkyGIE
K134-ub LkQIEIKkFkyGIEE
K136-ub QIEIKkFkyGIEEHG
Y137-p IEIKkFkyGIEEHGK
K144 yGIEEHGKVKMRGGL
R243 VKDRVKGRsDPyHAT
S244-p KDRVKGRsDPyHATT
Y247-p VKGRsDPyHATTGPL
T250 RsDPyHATTGPLsPs
T251 sDPyHATTGPLsPsk
S255-p HATTGPLsPskDCGs
S257-p TTGPLsPskDCGsPk
K258-ub TGPLsPskDCGsPky
S262-p sPskDCGsPkyAyFN
K264-ub skDCGsPkyAyFNGC
Y265-p kDCGsPkyAyFNGCS
Y267-p CGsPkyAyFNGCSSP
S273 AyFNGCSSPTAPLsP
T275 FNGCSSPTAPLsPMs
S279-p SSPTAPLsPMsPPGY
S282-p TAPLsPMsPPGYkLV
Y286 sPMsPPGYkLVTGDR
K287-ub PMsPPGYkLVTGDRN
T290 PPGYkLVTGDRNNss
S296-p VTGDRNNssCRNYNk
S297-p TGDRNNssCRNYNkQ
Y301 NNssCRNYNkQAsEQ
K303-ub ssCRNYNkQAsEQNW
S306-p RNYNkQAsEQNWANy
Y313-p sEQNWANysAEQNRM
S314-p EQNWANysAEQNRMG
S325-p NRMGQAGstIsNsHA
T326-p RMGQAGstIsNsHAQ
S328-p GQAGstIsNsHAQPF
S330-p AGstIsNsHAQPFDF
N341 PFDFPDDNQNAKKVA
A344 FPDDNQNAKKVAAGH
K345 PDDNQNAKKVAAGHE
K346 DDNQNAKKVAAGHEL
S364-p AIVDQRPssRAssRA
S365-p IVDQRPssRAssRAs
S368-p QRPssRAssRAssRP
S369-p RPssRAssRAssRPR
S372-p sRAssRAssRPRPDD
S373-p RAssRAssRPRPDDL
3511 : Phospho-Connexin 43 (Ser368) Antibody
  rabbit

 
S5 ___MGDWSALGKLLD
K9 GDWSALGKLLDKVQA
K108 RKEEKLNKKEEELKV
K109 KEEKLNKKEEELKVA
K128 VNVEMHLKQIEIKKF
K133 HLKQIEIKKFKYGIE
K134 LKQIEIKKFKYGIEE
K136 QIEIKKFKYGIEEHG
Y137 IEIKKFKYGIEEHGK
K144 YGIEEHGKVKMRGGL
K243 VKDRVKGKSDPYHAT
S244 KDRVKGKSDPYHATT
Y247 VKGKSDPYHATTGPL
T250 KSDPYHATTGPLSPS
T251 SDPYHATTGPLSPSK
S255 HATTGPLSPSKDCGS
S257 TTGPLSPSKDCGSPK
K258 TGPLSPSKDCGSPKY
S262 SPSKDCGSPKYAYFN
K264 SKDCGSPKYAYFNGC
Y265 KDCGSPKYAYFNGCS
Y267 CGSPKYAYFNGCSSP
S273 AYFNGCSSPTAPLSP
T275 FNGCSSPTAPLSPMS
S279 SSPTAPLSPMSPPGY
S282 TAPLSPMSPPGYKLV
Y286 SPMSPPGYKLVTGDR
K287 PMSPPGYKLVTGDRN
T290 PPGYKLVTGDRNNSS
S296 VTGDRNNSSCRNYNK
S297 TGDRNNSSCRNYNKQ
Y301 NNSSCRNYNKQASEQ
K303 SSCRNYNKQASEQNW
S306 RNYNKQASEQNWANY
Y313 SEQNWANYSAEQNRM
S314 EQNWANYSAEQNRMG
S325 NRMGQAGSTISNSHA
T326 RMGQAGSTISNSHAQ
S328 GQAGSTISNSHAQPF
S330 AGSTISNSHAQPFDF
N341 PFDFPDDNQNSKKLA
S344 FPDDNQNSKKLAAGH
K345 PDDNQNSKKLAAGHE
K346 DDNQNSKKLAAGHEL
S364 AIVDQRPSSRASsRA
S365 IVDQRPSSRASsRAS
S368 QRPSSRASsRASSRP
S369-p RPSSRASsRASSRPR
S372 SRASsRASSRPRPDD
S373 RASsRASSRPRPDDL
  hamster

 
S5 ___MGDWSALGKLLD
K9 GDWSALGKLLDKVQA
K108 RKEEKLNKKEEELKV
K109 KEEKLNKKEEELKVA
K128 ANVDMHLKQIEIKKF
K133 HLKQIEIKKFKYGIE
K134 LKQIEIKKFKYGIEE
K136 QIEIKKFKYGIEEHG
Y137 IEIKKFKYGIEEHGK
K144 YGIEEHGKVKMRGGL
R243 VKDRVKGRSDPYHAT
S244 KDRVKGRSDPYHATT
Y247 VKGRSDPYHATTGPL
T250 RSDPYHATTGPLNPS
T251 SDPYHATTGPLNPSK
N255 HATTGPLNPSKDCGS
S257 TTGPLNPSKDCGSPK
K258 TGPLNPSKDCGSPKY
S262 NPSKDCGSPKYAYFN
K264 SKDCGSPKYAYFNGC
Y265 KDCGSPKYAYFNGCS
Y267 CGSPKYAYFNGCSSP
S273 AYFNGCSSPTAPLSP
T275 FNGCSSPTAPLSPMS
S279 SSPTAPLSPMSPPGY
S282 TAPLSPMSPPGYKLV
Y286 SPMSPPGYKLVTGDR
K287 PMSPPGYKLVTGDRN
T290 PPGYKLVTGDRNNSS
S296 VTGDRNNSSCRNYNK
S297 TGDRNNSSCRNYNKQ
Y301 NNSSCRNYNKQASEQ
K303 SSCRNYNKQASEQNW
S306 RNYNKQASEQNWANY
Y313 SEQNWANYSAEQNRM
S314 EQNWANYSAEQNRMG
S325 NRMGQAGSTISNSHA
T326 RMGQAGSTISNSHAQ
S328 GQAGSTISNSHAQPF
S330 AGSTISNSHAQPFDF
N341 PFDFPDDNQNAKKIA
A344 FPDDNQNAKKIAAGH
K345 PDDNQNAKKIAAGHE
K346 DDNQNAKKIAAGHEL
S364 AIVDQRPSSRASSRA
S365 IVDQRPSSRASSRAS
S368 QRPSSRASSRASSRP
S369 RPSSRASSRASSRPR
S372 SRASSRASSRPRPDD
S373 RASSRASSRPRPDDL
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