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Protein Page:
Cofilin-1 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
Cofilin-1 a cytoskeletal protein that controls actin depolymerization. Has a 5-10-fold higher affinity for ATP-actin monomers than cofilin-1. May promote filament assembly rather than disassembly. Two alternatively spliced variants are described. Isoform b is expressed predominantly in skeletal muscle and heart, while isoform a is expressed in various tissues. Note: This description may include information from UniProtKB.
Protein type: Nuclear receptor co-regulator; Cytoskeletal protein; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 11q13
Cellular Component: extracellular space; cortical actin cytoskeleton; nuclear matrix; membrane; cytoplasm; nucleus
Molecular Function: protein binding; actin binding
Biological Process: platelet activation; actin filament depolymerization; axon guidance; positive regulation of actin filament depolymerization; response to virus; negative regulation of cell size; cytoskeleton organization and biogenesis; response to amino acid stimulus; protein amino acid phosphorylation; Rho protein signal transduction; neural fold formation; platelet degranulation; establishment of cell polarity; protein import into nucleus; regulation of cell morphogenesis; innate immune response; blood coagulation; actin cytoskeleton organization and biogenesis; neural crest cell migration; negative regulation of apoptosis
Reference #:  P23528 (UniProtKB)
Alt. Names/Synonyms: CFL; CFL1; COF1; cofilin 1 (non-muscle); Cofilin, non-muscle isoform; Cofilin-1; p18
Gene Symbols: CFL1
Molecular weight: 18,502 Da
Basal Isoelectric point: 8.22  Predict pI for various phosphorylation states
CST Pathways:  Actin Dynamics  |  Microtubule Dynamics  |  TGF-├č Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

Cofilin-1

Protein Structure Not Found.


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Sites Implicated In
apoptosis, inhibited: S3‑p
cell adhesion, altered: S3‑p
cell cycle regulation: S3‑p
cell motility, altered: S3‑p, Y68‑p
cytoskeletal reorganization: S3‑p, S23‑p, S24‑p, Y68‑p
activity, induced: S3‑p
activity, inhibited: S3‑p
intracellular localization: S3‑p
molecular association, regulation: S3‑p, S23‑p, S24‑p
phosphorylation: S3‑p
protein degradation: Y68‑p
ubiquitination: Y68‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
58 40 S3-p _____MAsGVAVsDG
0 3 S8-p MAsGVAVsDGVIkVF
0 4 K13-ac AVsDGVIkVFNDMkV
0 2 K13-ub AVsDGVIkVFNDMkV
0 5 K19-ac IkVFNDMkVRksstP
0 12 K19-ub IkVFNDMkVRksstP
0 5 K22-ub FNDMkVRksstPEEV
1 2 S23-p NDMkVRksstPEEVk
1 12 S24-p DMkVRksstPEEVkk
0 19 T25-p MkVRksstPEEVkkR
0 3 K30-ac sstPEEVkkRkKAVL
0 1 K30-ub sstPEEVkkRkKAVL
0 1 K31-ac stPEEVkkRkKAVLF
0 1 K31 stPEEVkKRkKAVLF
0 3 K33-ac PEEVkkRkKAVLFCL
0 9 S41-p KAVLFCLsEDkkNII
0 45 K44-ac LFCLsEDkkNIILEE
0 11 K44-ub LFCLsEDkkNIILEE
0 4 K45-ac FCLsEDkkNIILEEG
0 7 K45-ub FCLsEDkkNIILEEG
0 6 K53-ub NIILEEGkEILVGDV
0 1 T63-p LVGDVGQtVDDPyAt
1 470 Y68-p GQtVDDPyAtFVkML
0 1 A69 QtVDDPyAtFVkMLP
0 16 T70-p tVDDPyAtFVkMLPD
0 10 K73-ub DPyAtFVkMLPDkDC
0 1 K73-sc DPyAtFVkMLPDkDC
0 2 K73-ac DPyAtFVkMLPDkDC
0 2 K78-ub FVkMLPDkDCRyALy
0 2 K78-ac FVkMLPDkDCRyALy
0 54 Y82-p LPDkDCRyALyDAty
0 265 Y85-p kDCRyALyDAtyEtk
0 64 T88-p RyALyDAtyEtkESk
0 518 Y89-p yALyDAtyEtkESkk
0 8 T91-p LyDAtyEtkESkkED
0 99 K92-ub yDAtyEtkESkkEDL
0 2 K92-ac yDAtyEtkESkkEDL
0 8 K95-ub tyEtkESkkEDLVFI
0 8 K96-ac yEtkESkkEDLVFIF
0 1 K96-m3 yEtkESkkEDLVFIF
0 73 K112-ub APESAPLkSkMIyAS
0 57 K114-ub ESAPLkSkMIyASSk
0 1 K114-ac ESAPLkSkMIyASSk
0 193 Y117-p PLkSkMIyASSkDAI
0 4 K121-ac kMIyASSkDAIkkkL
0 5 K121-ub kMIyASSkDAIkkkL
0 1 K125-ac ASSkDAIkkkLTGIk
0 8 K126-ac SSkDAIkkkLTGIkH
0 8 K127-ac SkDAIkkkLTGIkHE
0 18 K132-ac kkkLTGIkHELQANC
0 9 K132-ub kkkLTGIkHELQANC
0 1919 Y140-p HELQANCyEEVkDRC
0 16 K144-ac ANCyEEVkDRCTLAE
0 100 K144-ub ANCyEEVkDRCTLAE
0 1 K144-sc ANCyEEVkDRCTLAE
0 1 T148 EEVkDRCTLAEKLGG
0 16 S156-p LAEKLGGsAVISLEG
0 26 K164-ub AVISLEGkPL_____
3311 : Phospho-Cofilin (Ser3) Antibody
3313 : Phospho-Cofilin (Ser3) (77G2) Rabbit mAb
  mouse

 
S3-p _____MAsGVAVsDG
S8-p MAsGVAVsDGVIKVF
K13 AVsDGVIKVFNDMkV
K13 AVsDGVIKVFNDMkV
K19-ac IKVFNDMkVRKSstP
K19-ub IKVFNDMkVRKSstP
K22 FNDMkVRKSstPEEV
S23 NDMkVRKSstPEEVK
S24-p DMkVRKSstPEEVKk
T25-p MkVRKSstPEEVKkR
K30 SstPEEVKkRkKAVL
K30 SstPEEVKkRkKAVL
K31 stPEEVKKRkKAVLF
K31-ub stPEEVKkRkKAVLF
K33-ac PEEVKkRkKAVLFCL
S41-p KAVLFCLsEDkkNII
K44-ac LFCLsEDkkNIILEE
K44-ub LFCLsEDkkNIILEE
K45 FCLsEDkKNIILEEG
K45-ub FCLsEDkkNIILEEG
K53 NIILEEGKEILVGDV
T63 LVGDVGQTVDDPytT
Y68-p GQTVDDPytTFVkML
T69-p QTVDDPytTFVkMLP
T70 TVDDPytTFVkMLPD
K73-ub DPytTFVkMLPDkDC
K73 DPytTFVKMLPDkDC
K73-ac DPytTFVkMLPDkDC
K78-ub FVkMLPDkDCRYALy
K78 FVkMLPDKDCRYALy
Y82 LPDkDCRYALyDAty
Y85-p kDCRYALyDAtyEtk
T88-p RYALyDAtyEtkESk
Y89-p YALyDAtyEtkESkk
T91-p LyDAtyEtkESkkED
K92-ub yDAtyEtkESkkEDL
K92-ac yDAtyEtkESkkEDL
K95-ub tyEtkESkkEDLVFI
K96-ac yEtkESkkEDLVFIF
K96 yEtkESkKEDLVFIF
K112-ub APENAPLkSkMIyAS
K114-ub ENAPLkSkMIyASSk
K114 ENAPLkSKMIyASSk
Y117-p PLkSkMIyASSkDAI
K121-ac kMIyASSkDAIKKKL
K121-ub kMIyASSkDAIKKKL
K125 ASSkDAIKKKLTGIK
K126 SSkDAIKKKLTGIKH
K127 SkDAIKKKLTGIKHE
K132 KKKLTGIKHELQANC
K132 KKKLTGIKHELQANC
Y140-p HELQANCyEEVkDRC
K144-ac ANCyEEVkDRCtLAE
K144-ub ANCyEEVkDRCtLAE
K144 ANCyEEVKDRCtLAE
T148-p EEVkDRCtLAEKLGG
S156-p LAEKLGGsAVISLEG
K164-ub AVISLEGkPL_____
3311 : Phospho-Cofilin (Ser3) Antibody
3313 : Phospho-Cofilin (Ser3) (77G2) Rabbit mAb
  rat

 
S3-p _____MAsGVAVsDG
S8-p MAsGVAVsDGVIkVF
K13-ac AVsDGVIkVFNDMkV
K13 AVsDGVIKVFNDMkV
K19-ac IkVFNDMkVRKSStP
K19 IkVFNDMKVRKSStP
K22 FNDMkVRKSStPEEV
S23 NDMkVRKSStPEEVk
S24 DMkVRKSStPEEVkK
T25-p MkVRKSStPEEVkKR
K30-ac SStPEEVkKRKKAVL
K30 SStPEEVKKRKKAVL
K31 StPEEVkKRKKAVLF
K31 StPEEVkKRKKAVLF
K33 PEEVkKRKKAVLFCL
S41 KAVLFCLSEDKKNII
K44 LFCLSEDKKNIILEE
K44 LFCLSEDKKNIILEE
K45 FCLSEDKKNIILEEG
K45 FCLSEDKKNIILEEG
K53-ub NIILEEGkEILVGDV
T63 LVGDVGQTVDDPyTt
Y68-p GQTVDDPyTtFVkML
T69 QTVDDPyTtFVkMLP
T70-p TVDDPyTtFVkMLPD
K73-ub DPyTtFVkMLPDKDC
K73 DPyTtFVKMLPDKDC
K73 DPyTtFVKMLPDKDC
K78 FVkMLPDKDCRYALY
K78 FVkMLPDKDCRYALY
Y82 LPDKDCRYALYDATy
Y85 KDCRYALYDATyETk
T88 RYALYDATyETkESK
Y89-p YALYDATyETkESKK
T91 LYDATyETkESKKED
K92 YDATyETKESKKEDL
K92-ac YDATyETkESKKEDL
K95 TyETkESKKEDLVFI
K96 yETkESKKEDLVFIF
K96 yETkESKKEDLVFIF
K112-ub APESAPLkSkMIYAS
K114-ub ESAPLkSkMIYASSk
K114-ac ESAPLkSkMIYASSk
Y117 PLkSkMIYASSkDAI
K121-ac kMIYASSkDAIKKKL
K121 kMIYASSKDAIKKKL
K125 ASSkDAIKKKLTGIK
K126 SSkDAIKKKLTGIKH
K127 SkDAIKKKLTGIKHE
K132 KKKLTGIKHELQANC
K132 KKKLTGIKHELQANC
Y140-p HELQANCyEEVKDRC
K144 ANCyEEVKDRCTLAE
K144 ANCyEEVKDRCTLAE
K144 ANCyEEVKDRCTLAE
T148 EEVKDRCTLAEKLGG
S156-p LAEKLGGsAVISLEG
K164 AVISLEGKPL_____
3311 : Phospho-Cofilin (Ser3) Antibody
3313 : Phospho-Cofilin (Ser3) (77G2) Rabbit mAb
  pig

 
S3-p _____MAsGVAVSDG
S8 MAsGVAVSDGVIKVF
K13 AVSDGVIKVFNDMKV
K13 AVSDGVIKVFNDMKV
K19 IKVFNDMKVRKSSTP
K19 IKVFNDMKVRKSSTP
K22 FNDMKVRKSSTPEEV
S23 NDMKVRKSSTPEEVK
S24 DMKVRKSSTPEEVKK
T25 MKVRKSSTPEEVKKR
K30 SSTPEEVKKRKKAVL
K30 SSTPEEVKKRKKAVL
K31 STPEEVKKRKKAVLF
K31 STPEEVKKRKKAVLF
K33 PEEVKKRKKAVLFCL
S41 KAVLFCLSEDKKNII
K44 LFCLSEDKKNIILEE
K44 LFCLSEDKKNIILEE
K45 FCLSEDKKNIILEEG
K45 FCLSEDKKNIILEEG
K53 NIILEEGKEILVGDV
T63 LVGDVGQTVDDPYAT
Y68 GQTVDDPYATFVKML
A69 QTVDDPYATFVKMLP
T70 TVDDPYATFVKMLPD
K73 DPYATFVKMLPDKDC
K73 DPYATFVKMLPDKDC
K73 DPYATFVKMLPDKDC
K78 FVKMLPDKDCRYALY
K78 FVKMLPDKDCRYALY
Y82 LPDKDCRYALYDATY
Y85 KDCRYALYDATYETK
T88 RYALYDATYETKESK
Y89 YALYDATYETKESKK
T91 LYDATYETKESKKED
K92 YDATYETKESKKEDL
K92 YDATYETKESKKEDL
K95 TYETKESKKEDLVFI
K96 YETKESKKEDLVFIF
K96 YETKESKKEDLVFIF
K112 APECAPLKSKMIYAS
K114 ECAPLKSKMIYASSK
K114 ECAPLKSKMIYASSK
Y117 PLKSKMIYASSKDAI
K121 KMIYASSKDAIKKKL
K121 KMIYASSKDAIKKKL
K125 ASSKDAIKKKLTGIK
K126 SSKDAIKKKLTGIKH
K127 SKDAIKKKLTGIKHE
K132 KKKLTGIKHELQANC
K132 KKKLTGIKHELQANC
Y140 HELQANCYEEVKDRC
K144 ANCYEEVKDRCTLAE
K144 ANCYEEVKDRCTLAE
K144 ANCYEEVKDRCTLAE
T148 EEVKDRCTLAEKLGG
S156 LAEKLGGSAVISLEG
K164 AVISLEGKPL_____
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